NL8103680A - Eukaryotisch autonoom replicerend segment. - Google Patents
Eukaryotisch autonoom replicerend segment. Download PDFInfo
- Publication number
- NL8103680A NL8103680A NL8103680A NL8103680A NL8103680A NL 8103680 A NL8103680 A NL 8103680A NL 8103680 A NL8103680 A NL 8103680A NL 8103680 A NL8103680 A NL 8103680A NL 8103680 A NL8103680 A NL 8103680A
- Authority
- NL
- Netherlands
- Prior art keywords
- ars
- hybrid
- gene
- eukaryotic
- host
- Prior art date
Links
- 230000003362 replicative effect Effects 0.000 title claims description 18
- 108090000623 proteins and genes Proteins 0.000 claims description 83
- 108020004414 DNA Proteins 0.000 claims description 64
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 47
- 238000000034 method Methods 0.000 claims description 31
- 235000020669 docosahexaenoic acid Nutrition 0.000 claims description 30
- 210000004027 cell Anatomy 0.000 claims description 28
- 210000000349 chromosome Anatomy 0.000 claims description 24
- 241000206602 Eukaryota Species 0.000 claims description 23
- 239000012634 fragment Substances 0.000 claims description 23
- 230000010354 integration Effects 0.000 claims description 21
- 230000014509 gene expression Effects 0.000 claims description 17
- 230000010076 replication Effects 0.000 claims description 15
- 210000003527 eukaryotic cell Anatomy 0.000 claims description 12
- 230000001131 transforming effect Effects 0.000 claims description 10
- 230000015572 biosynthetic process Effects 0.000 claims description 9
- 238000003780 insertion Methods 0.000 claims description 9
- 230000037431 insertion Effects 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 7
- 230000000295 complement effect Effects 0.000 claims description 6
- 230000002068 genetic effect Effects 0.000 claims description 6
- 210000005253 yeast cell Anatomy 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 5
- 241000251539 Vertebrata <Metazoa> Species 0.000 claims description 3
- 230000006696 biosynthetic metabolic pathway Effects 0.000 claims description 3
- 230000002255 enzymatic effect Effects 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 2
- 230000008878 coupling Effects 0.000 claims description 2
- 238000010168 coupling process Methods 0.000 claims description 2
- 238000005859 coupling reaction Methods 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims 3
- 238000001727 in vivo Methods 0.000 claims 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims 1
- 210000001520 comb Anatomy 0.000 claims 1
- 235000013305 food Nutrition 0.000 claims 1
- 238000013467 fragmentation Methods 0.000 claims 1
- 238000006062 fragmentation reaction Methods 0.000 claims 1
- 235000009973 maize Nutrition 0.000 claims 1
- 239000002207 metabolite Substances 0.000 claims 1
- 230000009466 transformation Effects 0.000 description 17
- 101150009006 HIS3 gene Proteins 0.000 description 16
- 101100394989 Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) hisI gene Proteins 0.000 description 14
- 239000013598 vector Substances 0.000 description 14
- 238000002360 preparation method Methods 0.000 description 11
- 238000012217 deletion Methods 0.000 description 10
- 230000037430 deletion Effects 0.000 description 10
- 239000003550 marker Substances 0.000 description 10
- 239000013612 plasmid Substances 0.000 description 9
- 108091008146 restriction endonucleases Proteins 0.000 description 8
- 238000009396 hybridization Methods 0.000 description 7
- 230000035772 mutation Effects 0.000 description 7
- 238000003776 cleavage reaction Methods 0.000 description 6
- 230000007017 scission Effects 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 230000006798 recombination Effects 0.000 description 5
- 238000005215 recombination Methods 0.000 description 5
- 101100285000 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) his-3 gene Proteins 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 4
- 238000007792 addition Methods 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 238000001962 electrophoresis Methods 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 108010042407 Endonucleases Proteins 0.000 description 3
- 102000004533 Endonucleases Human genes 0.000 description 3
- 108060002716 Exonuclease Proteins 0.000 description 3
- 102000003960 Ligases Human genes 0.000 description 3
- 108090000364 Ligases Proteins 0.000 description 3
- 241001599018 Melanogaster Species 0.000 description 3
- 239000000020 Nitrocellulose Substances 0.000 description 3
- 229960000723 ampicillin Drugs 0.000 description 3
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 229940088710 antibiotic agent Drugs 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 210000000941 bile Anatomy 0.000 description 3
- 102000013165 exonuclease Human genes 0.000 description 3
- 229920001220 nitrocellulos Polymers 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 2
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 101100479039 Caenorhabditis elegans aars-1 gene Proteins 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- 241000221961 Neurospora crassa Species 0.000 description 2
- 239000004098 Tetracycline Substances 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 230000002567 autonomic effect Effects 0.000 description 2
- 238000000376 autoradiography Methods 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000013611 chromosomal DNA Substances 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 108010056929 lyticase Proteins 0.000 description 2
- 239000006152 selective media Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 229960002180 tetracycline Drugs 0.000 description 2
- 229930101283 tetracycline Natural products 0.000 description 2
- 235000019364 tetracycline Nutrition 0.000 description 2
- 150000003522 tetracyclines Chemical class 0.000 description 2
- 238000000844 transformation Methods 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 229940035893 uracil Drugs 0.000 description 2
- 210000002845 virion Anatomy 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- NFGXHKASABOEEW-UHFFFAOYSA-N 1-methylethyl 11-methoxy-3,7,11-trimethyl-2,4-dodecadienoate Chemical compound COC(C)(C)CCCC(C)CC=CC(C)=CC(=O)OC(C)C NFGXHKASABOEEW-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 101150029129 AR gene Proteins 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 101100148606 Caenorhabditis elegans pst-1 gene Proteins 0.000 description 1
- 235000007575 Calluna vulgaris Nutrition 0.000 description 1
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 description 1
- 241000579120 Coliiformes Species 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 241001362614 Crassa Species 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- 241000168726 Dictyostelium discoideum Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241001302584 Escherichia coli str. K-12 substr. W3110 Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 108010001496 Galectin 2 Proteins 0.000 description 1
- 102100024637 Galectin-10 Human genes 0.000 description 1
- 102100021735 Galectin-2 Human genes 0.000 description 1
- 101001011019 Gallus gallus Gallinacin-10 Proteins 0.000 description 1
- 101001011021 Gallus gallus Gallinacin-12 Proteins 0.000 description 1
- 229920011425 Gentro Polymers 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 102000011782 Keratins Human genes 0.000 description 1
- 108010076876 Keratins Proteins 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 101100281686 Mus musculus Fstl1 gene Proteins 0.000 description 1
- 108010047956 Nucleosomes Proteins 0.000 description 1
- 108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
- 102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 1
- 108091036333 Rapid DNA Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 101100097319 Schizosaccharomyces pombe (strain 972 / ATCC 24843) ala1 gene Proteins 0.000 description 1
- 101150073336 Sycp1 gene Proteins 0.000 description 1
- 101150006914 TRP1 gene Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 1
- 229950006790 adenosine phosphate Drugs 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- -1 anti-cellin Natural products 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- GYOZYWVXFNDGLU-XLPZGREQSA-N dTMP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)C1 GYOZYWVXFNDGLU-XLPZGREQSA-N 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 150000002009 diols Chemical class 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- ZDPUTNZENXVHJC-UUOKFMHZSA-N guanosine 3'-monophosphate Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](OP(O)(O)=O)[C@H]1O ZDPUTNZENXVHJC-UUOKFMHZSA-N 0.000 description 1
- 235000013928 guanylic acid Nutrition 0.000 description 1
- 239000004226 guanylic acid Substances 0.000 description 1
- 230000026030 halogenation Effects 0.000 description 1
- 238000005658 halogenation reaction Methods 0.000 description 1
- 101150107671 hisB gene Proteins 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 150000004678 hydrides Chemical class 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000000394 mitotic effect Effects 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 210000001623 nucleosome Anatomy 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 101150116440 pyrF gene Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000006722 reduction reaction Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000032537 response to toxin Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 239000007320 rich medium Substances 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000013638 trimer Substances 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/68—Stabilisation of the vector
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17554780A | 1980-08-05 | 1980-08-05 | |
| US17554780 | 1980-08-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NL8103680A true NL8103680A (nl) | 1982-03-01 |
Family
ID=22640660
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NL8103680A NL8103680A (nl) | 1980-08-05 | 1981-08-04 | Eukaryotisch autonoom replicerend segment. |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0045573A3 (fr) |
| JP (1) | JPS57163395A (fr) |
| CA (1) | CA1202918A (fr) |
| DE (1) | DE3131034A1 (fr) |
| IL (1) | IL63270A0 (fr) |
| NL (1) | NL8103680A (fr) |
| SE (1) | SE8104700L (fr) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4508823A (en) * | 1980-05-08 | 1985-04-02 | Microlife Technics, Inc. | Gene splicing method and products produced therefrom |
| JPS57129691A (en) * | 1980-09-22 | 1982-08-11 | Yunibaashiteii Obu Sazan Karif | Rearranged dna active in nuclear cell and preparation thereof |
| DE3381090D1 (de) * | 1982-05-19 | 1990-02-15 | Gist Brocades Nv | Klonierungssystem fuer kluyveromyce spezies. |
| GB2125047B (en) * | 1982-08-09 | 1986-02-19 | Ciba Geigy Ag | Yeast hybrid vectors and their use for the production of polypeptides |
| NO172595C (no) * | 1983-02-07 | 1993-08-11 | Battelle Memorial Institute | Fremgangsmaate og ekspresjonsplasmid for fremstilling av hemagglutinin under kontroll av drosophila hsp-promoter |
| NZ207765A (en) * | 1983-04-15 | 1987-03-06 | Lubrizol Genetics Inc | Plant expression of transferred dna(t-dna)from plasmids associated with agrobacterium sp |
| US5639639A (en) * | 1983-11-02 | 1997-06-17 | Genzyme Corporation | Recombinant heterodimeric human fertility hormones, and methods, cells, vectors and DNA for the production thereof |
| ATE136057T1 (de) * | 1986-07-24 | 1996-04-15 | Daiichi Seiyaku Co | Autonom replizierende dna fragmente aus säugerzellen mit affinität zu dna bindenden proteinen |
| US5364761A (en) * | 1986-07-24 | 1994-11-15 | Daiichi Pharmaceutical Co., Ltd. | Method for isolating a DNA encoding an autonomously replicating sequence |
| CA1340772C (fr) | 1987-12-30 | 1999-09-28 | Patricia Tekamp-Olson | Expression et secretion de proteines heterologues dans une levure au moyen de sequences leader tronquees du facteur alpha |
| FR2626584B1 (fr) * | 1988-01-28 | 1990-07-13 | Agronomique Inst Nat Rech | Sequence ars efficace chez yarrowia lipolytica et procede pour sa preparation |
| EP2042196B1 (fr) | 2001-10-10 | 2016-07-13 | ratiopharm GmbH | Remodelage et glycoconjugation de facteur stimulant les colonies de granulocytes (G-CSF) |
| NZ542586A (en) | 2003-04-09 | 2009-11-27 | Biogenerix Ag | Method of forming a covalent conjugate between a G-CSF peptide and PEG |
| EP2380905A1 (fr) | 2010-04-19 | 2011-10-26 | Thrombotargets Europe, S.L. | Vésicules enrichies de phospholipides portant un facteur tissulaire doté d'activités hémostatiques et utilisations correspondantes |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL63035A (en) * | 1980-09-09 | 1985-05-31 | Univ California | Dnas capable of replication and stable mitotic maintenance in a host eukaryote,their preparation and eukaryotic cells comprising them |
| JPS57129691A (en) * | 1980-09-22 | 1982-08-11 | Yunibaashiteii Obu Sazan Karif | Rearranged dna active in nuclear cell and preparation thereof |
-
1981
- 1981-07-09 IL IL63270A patent/IL63270A0/xx unknown
- 1981-07-10 EP EP81303155A patent/EP0045573A3/fr not_active Withdrawn
- 1981-07-23 CA CA000382368A patent/CA1202918A/fr not_active Expired
- 1981-08-04 NL NL8103680A patent/NL8103680A/nl not_active Application Discontinuation
- 1981-08-05 SE SE8104700A patent/SE8104700L/ not_active Application Discontinuation
- 1981-08-05 JP JP56121992A patent/JPS57163395A/ja active Pending
- 1981-08-05 DE DE19813131034 patent/DE3131034A1/de not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| SE8104700L (sv) | 1982-02-06 |
| CA1202918A (fr) | 1986-04-08 |
| EP0045573A2 (fr) | 1982-02-10 |
| JPS57163395A (en) | 1982-10-07 |
| IL63270A0 (en) | 1981-10-30 |
| DE3131034A1 (de) | 1982-06-16 |
| EP0045573A3 (fr) | 1982-12-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5869239A (en) | Library screening method | |
| Clarke et al. | Functional expression of cloned yeast DNA in Escherichia coli: specific complementation of argininosuccinate lyase (argH) mutations | |
| US6573046B1 (en) | Eukaryotic use of improved chimeric mutational vectors | |
| JP2552809B2 (ja) | 自律複製配列を有するｄｎａ断片、同断片を含有するハイブリッドプラスミド、同ハイブリッドプラスミドにより形質転換した大腸菌及び当該ｄｎａ断片の製造方法 | |
| US5783385A (en) | Method for homologous-recombination screening of recombinant-DNA clones in yeast host libraries | |
| US5212087A (en) | Ars sequence which is efficacious in yarrowia lipolytica | |
| US6232112B1 (en) | Reagents and methods for diversification of DNA | |
| EP0045573A2 (fr) | Segment eucaryotique à replication autonome | |
| US7115413B2 (en) | Meiotic recombination in vivo of partially homologous DNA sequences | |
| Paietta et al. | Molecular cloning and characterization of the cys-3 regulatory gene of Neurospora crassa | |
| AU8171087A (en) | Gene amplification using retrotransposons | |
| US4464472A (en) | Construction and use of functional minichromosomes | |
| US4477571A (en) | Chimeric plasmids that replicate in bacteria and yeast and microorganisms transformed therewith | |
| EP0048081A2 (fr) | ADN capable de se répliquer et de se maintenir de façon stable en mitose dans un hôte eucaryotique, méthode de préparation et cellule eucaryotique le contenant | |
| CN102292442A (zh) | 粟酒裂殖酵母的转化方法、转化体以及异源蛋白质的制造方法 | |
| EP0596885B1 (fr) | Methode de criblage de librairie d'adn | |
| Case | Selective deletion of large segments of Balbiani ring DNA during molecular cloning | |
| JPH07289262A (ja) | 部位特異的変異導入方法 | |
| JP2021019565A (ja) | アスペルギルス属糸状菌のピリチアミン耐性変異株 | |
| Symington et al. | Genetic recombination catalyzed by cell-free extracts of Saccharomyces cerevisiae | |
| EP0472286A1 (fr) | Gène codant pour l'H+ATPase de la membrane plasmatique de Candida albicans | |
| Ferencz | Using DNA Looping Proteins to Enhance Homology Directed Repair In Vivo Following a Cas9 Induced Double Strand Break | |
| WO2024122646A1 (fr) | Procédé pour produire des cellules à génome modifié et procédé pour favoriser l'hybridation | |
| CN113462681A (zh) | 一种构建dna片段敲除文库的方法 | |
| Lee | Characterization of two cysteine transfer RNA genes from Xenopus Laevis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| BV | The patent application has lapsed |