NL9301957A - Werkwijze voor het identificeren van micro-organismen, en daarvoor bruikbare hulpmiddelen. - Google Patents

Werkwijze voor het identificeren van micro-organismen, en daarvoor bruikbare hulpmiddelen. Download PDF

Info

Publication number: NL9301957A
Authority: NL; Netherlands
Prior art keywords: nucleic acid; primers; amplification; dna; electrophoresis
Prior art date: 1993-11-11

Application number

NL9301957A

Other languages

English (en)

Dutch (nl)

Original Assignee

U Gene Research Bv

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1993-11-11

Filing date

1993-11-11

Publication date

1995-06-01

1993-11-11 Application filed by U Gene Research Bv filed Critical U Gene Research Bv

1993-11-11 Priority to NL9301957A priority Critical patent/NL9301957A/nl

1994-11-11 Priority to PCT/NL1994/000283 priority patent/WO1995013396A2/fr

1995-06-01 Publication of NL9301957A publication Critical patent/NL9301957A/nl

Links

238000000034 method Methods 0.000 title claims description 66
244000005700 microbiome Species 0.000 title claims description 33
108020004414 DNA Proteins 0.000 claims description 65
102000039446 nucleic acids Human genes 0.000 claims description 54
108020004707 nucleic acids Proteins 0.000 claims description 54
150000007523 nucleic acids Chemical class 0.000 claims description 54
238000003199 nucleic acid amplification method Methods 0.000 claims description 52
230000003321 amplification Effects 0.000 claims description 51
108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 36
102000053602 DNA Human genes 0.000 claims description 29
238000003752 polymerase chain reaction Methods 0.000 claims description 27
241000894006 Bacteria Species 0.000 claims description 21
238000001962 electrophoresis Methods 0.000 claims description 20
239000002773 nucleotide Substances 0.000 claims description 18
125000003729 nucleotide group Chemical group 0.000 claims description 18
108090000623 proteins and genes Proteins 0.000 claims description 15
238000001514 detection method Methods 0.000 claims description 13
108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 12
102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 12
239000002299 complementary DNA Substances 0.000 claims description 11
238000010186 staining Methods 0.000 claims description 10
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 8
108020004465 16S ribosomal RNA Proteins 0.000 claims description 8
238000006243 chemical reaction Methods 0.000 claims description 8
210000004369 blood Anatomy 0.000 claims description 7
239000008280 blood Substances 0.000 claims description 7
238000013518 transcription Methods 0.000 claims description 7
230000035897 transcription Effects 0.000 claims description 7
208000002109 Argyria Diseases 0.000 claims description 6
BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 claims description 6
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 claims description 6
229960005542 ethidium bromide Drugs 0.000 claims description 6
241000233866 Fungi Species 0.000 claims description 5
241000700605 Viruses Species 0.000 claims description 5
230000010076 replication Effects 0.000 claims description 5
SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 claims description 4
239000002253 acid Substances 0.000 claims description 4
239000011616 biotin Substances 0.000 claims description 4
229960002685 biotin Drugs 0.000 claims description 4
235000020958 biotin Nutrition 0.000 claims description 4
QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 claims description 4
SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 claims description 4
244000045947 parasite Species 0.000 claims description 4
241000186361 Actinobacteria <class> Species 0.000 claims description 3
206010036790 Productive cough Diseases 0.000 claims description 3
108010006785 Taq Polymerase Proteins 0.000 claims description 3
108010001244 Tli polymerase Proteins 0.000 claims description 3
108010020713 Tth polymerase Proteins 0.000 claims description 3
MPBRYMWMMKKRGC-UHFFFAOYSA-M carbocyanin DBTC Chemical compound [Br-].C1=CC=CC2=C([N+](=C(C=C(C)C=C3N(C4=C5C=CC=CC5=CC=C4S3)CC)S3)CC)C3=CC=C21 MPBRYMWMMKKRGC-UHFFFAOYSA-M 0.000 claims description 3
210000003608 fece Anatomy 0.000 claims description 3
235000013305 food Nutrition 0.000 claims description 3
238000002264 polyacrylamide gel electrophoresis Methods 0.000 claims description 3
238000010839 reverse transcription Methods 0.000 claims description 3
210000003802 sputum Anatomy 0.000 claims description 3
208000024794 sputum Diseases 0.000 claims description 3
210000002700 urine Anatomy 0.000 claims description 3
NOIIUHRQUVNIDD-UHFFFAOYSA-N 3-[[oxo(pyridin-4-yl)methyl]hydrazo]-N-(phenylmethyl)propanamide Chemical compound C=1C=CC=CC=1CNC(=O)CCNNC(=O)C1=CC=NC=C1 NOIIUHRQUVNIDD-UHFFFAOYSA-N 0.000 claims description 2
238000012197 amplification kit Methods 0.000 claims description 2
230000001404 mediated effect Effects 0.000 claims description 2
239000000126 substance Substances 0.000 claims description 2
241000894007 species Species 0.000 description 31
239000000499 gel Substances 0.000 description 20
239000000523 sample Substances 0.000 description 19
108020004682 Single-Stranded DNA Proteins 0.000 description 18
230000001580 bacterial effect Effects 0.000 description 16
230000037230 mobility Effects 0.000 description 16
230000035772 mutation Effects 0.000 description 12
239000000047 product Substances 0.000 description 12
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 9
102100034343 Integrase Human genes 0.000 description 9
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 7
238000004458 analytical method Methods 0.000 description 7
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
239000008367 deionised water Substances 0.000 description 6
229910021641 deionized water Inorganic materials 0.000 description 6
238000010438 heat treatment Methods 0.000 description 6
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
241000588724 Escherichia coli Species 0.000 description 5
230000008859 change Effects 0.000 description 5
238000002360 preparation method Methods 0.000 description 5
208000031729 Bacteremia Diseases 0.000 description 4
239000003298 DNA probe Substances 0.000 description 4
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
241001138501 Salmonella enterica Species 0.000 description 4
ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 description 4
230000008901 benefit Effects 0.000 description 4
210000004027 cell Anatomy 0.000 description 4
KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 4
238000011161 development Methods 0.000 description 4
239000012634 fragment Substances 0.000 description 4
238000001502 gel electrophoresis Methods 0.000 description 4
238000009396 hybridization Methods 0.000 description 4
BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
230000035945 sensitivity Effects 0.000 description 4
SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 4
BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 3
241000588914 Enterobacter Species 0.000 description 3
102000004190 Enzymes Human genes 0.000 description 3
108090000790 Enzymes Proteins 0.000 description 3
WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 3
108091028043 Nucleic acid sequence Proteins 0.000 description 3
241000588769 Proteus <enterobacteria> Species 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 3
101710136739 Teichoic acid poly(glycerol phosphate) polymerase Proteins 0.000 description 3
239000007983 Tris buffer Substances 0.000 description 3
238000011534 incubation Methods 0.000 description 3
239000000203 mixture Substances 0.000 description 3
229920002401 polyacrylamide Polymers 0.000 description 3
239000000243 solution Substances 0.000 description 3
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
OSBLTNPMIGYQGY-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid Chemical compound OB(O)O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O OSBLTNPMIGYQGY-UHFFFAOYSA-N 0.000 description 2
OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 2
FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
238000002965 ELISA Methods 0.000 description 2
241000206602 Eukaryota Species 0.000 description 2
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
108010010803 Gelatin Proteins 0.000 description 2
208000031886 HIV Infections Diseases 0.000 description 2
241000701806 Human papillomavirus Species 0.000 description 2
101710203526 Integrase Proteins 0.000 description 2
241000186805 Listeria innocua Species 0.000 description 2
241000186779 Listeria monocytogenes Species 0.000 description 2
108091034117 Oligonucleotide Proteins 0.000 description 2
238000012408 PCR amplification Methods 0.000 description 2
241000224016 Plasmodium Species 0.000 description 2
241000589776 Pseudomonas putida Species 0.000 description 2
102000006382 Ribonucleases Human genes 0.000 description 2
108010083644 Ribonucleases Proteins 0.000 description 2
241000607768 Shigella Species 0.000 description 2
CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
241000191967 Staphylococcus aureus Species 0.000 description 2
239000008051 TBE buffer Substances 0.000 description 2
229910001870 ammonium persulfate Inorganic materials 0.000 description 2
238000000211 autoradiogram Methods 0.000 description 2
238000011109 contamination Methods 0.000 description 2
230000009089 cytolysis Effects 0.000 description 2
238000000326 densiometry Methods 0.000 description 2
230000001419 dependent effect Effects 0.000 description 2
230000004069 differentiation Effects 0.000 description 2
230000000694 effects Effects 0.000 description 2
MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 2
235000019253 formic acid Nutrition 0.000 description 2
239000008273 gelatin Substances 0.000 description 2
229920000159 gelatin Polymers 0.000 description 2
235000019322 gelatine Nutrition 0.000 description 2
235000011852 gelatine desserts Nutrition 0.000 description 2
230000002068 genetic effect Effects 0.000 description 2
238000000338 in vitro Methods 0.000 description 2
238000003780 insertion Methods 0.000 description 2
230000037431 insertion Effects 0.000 description 2
238000002372 labelling Methods 0.000 description 2
229910001629 magnesium chloride Inorganic materials 0.000 description 2
239000012528 membrane Substances 0.000 description 2
238000012986 modification Methods 0.000 description 2
230000004048 modification Effects 0.000 description 2
239000008188 pellet Substances 0.000 description 2
102000054765 polymorphisms of proteins Human genes 0.000 description 2
230000008569 process Effects 0.000 description 2
229910001961 silver nitrate Inorganic materials 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
125000006850 spacer group Chemical group 0.000 description 2
238000007447 staining method Methods 0.000 description 2
239000007858 starting material Substances 0.000 description 2
241001624918 unidentified bacterium Species 0.000 description 2
DDGHBOLOCQWPKE-UHFFFAOYSA-N 1,3-thiazole;hydrobromide Chemical compound [Br-].C1=CSC=[NH+]1 DDGHBOLOCQWPKE-UHFFFAOYSA-N 0.000 description 1
KJCVRFUGPWSIIH-UHFFFAOYSA-N 1-naphthol Chemical compound C1=CC=C2C(O)=CC=CC2=C1 KJCVRFUGPWSIIH-UHFFFAOYSA-N 0.000 description 1
BHNQPLPANNDEGL-UHFFFAOYSA-N 2-(4-octylphenoxy)ethanol Chemical compound CCCCCCCCC1=CC=C(OCCO)C=C1 BHNQPLPANNDEGL-UHFFFAOYSA-N 0.000 description 1
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
241000589291 Acinetobacter Species 0.000 description 1
HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
241000713838 Avian myeloblastosis virus Species 0.000 description 1
108090001008 Avidin Proteins 0.000 description 1
241000606124 Bacteroides fragilis Species 0.000 description 1
CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
GAMDYKWKZCXVST-UHFFFAOYSA-N C1=CC=C2C(CC)(O)CC=CC2=C1 Chemical compound C1=CC=C2C(CC)(O)CC=CC2=C1 GAMDYKWKZCXVST-UHFFFAOYSA-N 0.000 description 1
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
241001112695 Clostridiales Species 0.000 description 1
241000193163 Clostridioides difficile Species 0.000 description 1
230000006820 DNA synthesis Effects 0.000 description 1
102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
102000007260 Deoxyribonuclease I Human genes 0.000 description 1
108010008532 Deoxyribonuclease I Proteins 0.000 description 1
102000016911 Deoxyribonucleases Human genes 0.000 description 1
108010053770 Deoxyribonucleases Proteins 0.000 description 1
241000588697 Enterobacter cloacae Species 0.000 description 1
241000709661 Enterovirus Species 0.000 description 1
241000192125 Firmicutes Species 0.000 description 1
241000282412 Homo Species 0.000 description 1
241000725303 Human immunodeficiency virus Species 0.000 description 1
241000713772 Human immunodeficiency virus 1 Species 0.000 description 1
101000829171 Hypocrea virens (strain Gv29-8 / FGSC 10586) Effector TSP1 Proteins 0.000 description 1
208000026350 Inborn Genetic disease Diseases 0.000 description 1
241000588915 Klebsiella aerogenes Species 0.000 description 1
241000588749 Klebsiella oxytoca Species 0.000 description 1
241000588747 Klebsiella pneumoniae Species 0.000 description 1
241000186781 Listeria Species 0.000 description 1
208000024556 Mendelian disease Diseases 0.000 description 1
208000007101 Muscle Cramp Diseases 0.000 description 1
241000187479 Mycobacterium tuberculosis Species 0.000 description 1
239000000020 Nitrocellulose Substances 0.000 description 1
239000004677 Nylon Substances 0.000 description 1
108700020796 Oncogene Proteins 0.000 description 1
102000043276 Oncogene Human genes 0.000 description 1
238000002944 PCR assay Methods 0.000 description 1
241000588912 Pantoea agglomerans Species 0.000 description 1
241000589516 Pseudomonas Species 0.000 description 1
241000589517 Pseudomonas aeruginosa Species 0.000 description 1
241000589540 Pseudomonas fluorescens Species 0.000 description 1
241000446766 Shigella dysenteriae 1 Species 0.000 description 1
241000607762 Shigella flexneri Species 0.000 description 1
BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
241000191940 Staphylococcus Species 0.000 description 1
101710137500 T7 RNA polymerase Proteins 0.000 description 1
PLGSVAFGXVQXPI-UHFFFAOYSA-M [F-].[Cr+] Chemical compound [F-].[Cr+] PLGSVAFGXVQXPI-UHFFFAOYSA-M 0.000 description 1
DZBUGLKDJFMEHC-UHFFFAOYSA-N acridine Chemical class C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
235000011114 ammonium hydroxide Nutrition 0.000 description 1
239000003242 anti bacterial agent Substances 0.000 description 1
229940088710 antibiotic agent Drugs 0.000 description 1
238000011203 antimicrobial therapy Methods 0.000 description 1
238000003556 assay Methods 0.000 description 1
238000000376 autoradiography Methods 0.000 description 1
239000011324 bead Substances 0.000 description 1
239000006161 blood agar Substances 0.000 description 1
210000000601 blood cell Anatomy 0.000 description 1
UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 1
239000000872 buffer Substances 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
230000000295 complement effect Effects 0.000 description 1
108091036078 conserved sequence Proteins 0.000 description 1
238000012136 culture method Methods 0.000 description 1
125000004122 cyclic group Chemical group 0.000 description 1
SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
238000013503 de-identification Methods 0.000 description 1
238000004925 denaturation Methods 0.000 description 1
230000036425 denaturation Effects 0.000 description 1
238000003936 denaturing gel electrophoresis Methods 0.000 description 1
238000013461 design Methods 0.000 description 1
238000003745 diagnosis Methods 0.000 description 1
231100000676 disease causative agent Toxicity 0.000 description 1
229940079593 drug Drugs 0.000 description 1
239000003814 drug Substances 0.000 description 1
229940092559 enterobacter aerogenes Drugs 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
238000012252 genetic analysis Methods 0.000 description 1
230000001900 immune effect Effects 0.000 description 1
238000003018 immunoassay Methods 0.000 description 1
230000006872 improvement Effects 0.000 description 1
208000015181 infectious disease Diseases 0.000 description 1
239000003112 inhibitor Substances 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
238000002955 isolation Methods 0.000 description 1
238000007403 mPCR Methods 0.000 description 1
201000004792 malaria Diseases 0.000 description 1
238000012544 monitoring process Methods 0.000 description 1
ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 1
238000007857 nested PCR Methods 0.000 description 1
229920001220 nitrocellulos Polymers 0.000 description 1
229920001778 nylon Polymers 0.000 description 1
230000005855 radiation Effects 0.000 description 1
230000002285 radioactive effect Effects 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
238000011160 research Methods 0.000 description 1
230000001177 retroviral effect Effects 0.000 description 1
210000003705 ribosome Anatomy 0.000 description 1
239000012723 sample buffer Substances 0.000 description 1
238000012216 screening Methods 0.000 description 1
239000006152 selective media Substances 0.000 description 1
238000012163 sequencing technique Methods 0.000 description 1
229910052709 silver Inorganic materials 0.000 description 1
239000004332 silver Substances 0.000 description 1
229910000029 sodium carbonate Inorganic materials 0.000 description 1
VVLFAAMTGMGYBS-UHFFFAOYSA-M sodium;4-[[4-(ethylamino)-3-methylphenyl]-(4-ethylimino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-3-sulfobenzenesulfonate Chemical compound [Na+].C1=C(C)C(NCC)=CC=C1C(C=1C(=CC(=CC=1)S([O-])(=O)=O)S(O)(=O)=O)=C1C=C(C)C(=NCC)C=C1 VVLFAAMTGMGYBS-UHFFFAOYSA-M 0.000 description 1
239000000758 substrate Substances 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
239000000725 suspension Substances 0.000 description 1
238000010257 thawing Methods 0.000 description 1
239000001226 triphosphate Substances 0.000 description 1
235000011178 triphosphate Nutrition 0.000 description 1
125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
241000701161 unidentified adenovirus Species 0.000 description 1
108700026220 vif Genes Proteins 0.000 description 1
230000003612 virological effect Effects 0.000 description 1
230000001018 virulence Effects 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Analytical Chemistry (AREA)
Biophysics (AREA)
Immunology (AREA)
Microbiology (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Chemical Kinetics & Catalysis (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

NL9301957A 1993-11-11 1993-11-11 Werkwijze voor het identificeren van micro-organismen, en daarvoor bruikbare hulpmiddelen. NL9301957A (nl)

Priority Applications (2)

Application Number	Priority Date	Filing Date	Title
NL9301957A NL9301957A (nl)	1993-11-11	1993-11-11	Werkwijze voor het identificeren van micro-organismen, en daarvoor bruikbare hulpmiddelen.
PCT/NL1994/000283 WO1995013396A2 (fr)	1993-11-11	1994-11-11	Procede d'identification de micro-organismes, et dispositifs pour sa mise en oeuvre

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
NL9301957		1993-11-11
NL9301957A NL9301957A (nl)	1993-11-11	1993-11-11	Werkwijze voor het identificeren van micro-organismen, en daarvoor bruikbare hulpmiddelen.

Publications (1)

Publication Number	Publication Date
NL9301957A true NL9301957A (nl)	1995-06-01

Family

ID=19863128

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NL9301957A NL9301957A (nl)	1993-11-11	1993-11-11	Werkwijze voor het identificeren van micro-organismen, en daarvoor bruikbare hulpmiddelen.

Country Status (2)

Country	Link
NL (1)	NL9301957A (fr)
WO (1)	WO1995013396A2 (fr)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5942391A (en)	1994-06-22	1999-08-24	Mount Sinai School Of Medicine	Nucleic acid amplification method: ramification-extension amplification method (RAM)
US6593086B2 (en)	1996-05-20	2003-07-15	Mount Sinai School Of Medicine Of New York University	Nucleic acid amplification methods
USRE38442E1 (en) *	1994-06-22	2004-02-24	Mount Sinai School Of Medicine	Nucleic acid amplification method hybridization signal amplification method (HSAM)
US5876924A (en) *	1994-06-22	1999-03-02	Mount Sinai School Of Medicine	Nucleic acid amplification method hybridization signal amplification method (HSAM)
GB9420774D0 (en) *	1994-10-14	1994-11-30	Univ Wales	Method of detecting and identifying spore forming bacteria
DE19716456C2 (de) *	1997-04-21	2002-05-08	Schmitt Heinz Josef	Verwendung einer Kombination von Primern zum Nachweis von Erregern von Infektionskrankheiten
GB9719044D0 (en) *	1997-09-08	1997-11-12	Inst Of Ophthalmology	Assay
EP1044284A2 (fr) *	1998-01-08	2000-10-18	Merck Sharp & Dohme de Espana S.A.E.	Sondes d'hybridation pouvant faire la differenciation entre le groupe de bacteries actinomadura madurae et les bacteries maduromycetes
AU5062500A (en) *	1999-04-26	2000-11-10	Amersham Pharmacia Biotech Ab	Primers for identifying typing or classifying nucleic acids
DE19945916A1 (de) *	1999-09-24	2001-04-05	Biotecon Diagnostics Gmbh	Nukleinsäuremoleküle zum Nachweis von Bakterien und phylogenetischen Einheiten von Bakterien
WO2001023608A2 (fr) *	1999-09-27	2001-04-05	Merck Sharp & Dohme De Espana, S.A.E.	Sondes d'hybridation permettant de detecter de maniere specifique des souches du genre microbispora, microtetraspora, nonomuria et planobispora
DE10027113A1 (de)	1999-12-23	2001-09-27	Andreas Hoeft	Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
US7666588B2 (en)	2001-03-02	2010-02-23	Ibis Biosciences, Inc.	Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
WO2004060278A2 (fr)	2002-12-06	2004-07-22	Isis Pharmaceuticals, Inc.	Procedes d'identification rapide de pathogenes chez l'homme et les betes
US20040121309A1 (en)	2002-12-06	2004-06-24	Ecker David J.	Methods for rapid detection and identification of bioagents in blood, bodily fluids, and bodily tissues
AU2013231102B2 (en) *	2001-03-02	2016-04-28	Ibis Biosciences, Inc.	Methods for rapid identification of pathogens in humans and animals
US20030027135A1 (en)	2001-03-02	2003-02-06	Ecker David J.	Method for rapid detection and identification of bioagents
US7226739B2 (en)	2001-03-02	2007-06-05	Isis Pharmaceuticals, Inc	Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
US7217510B2 (en) *	2001-06-26	2007-05-15	Isis Pharmaceuticals, Inc.	Methods for providing bacterial bioagent characterizing information
US8057993B2 (en)	2003-04-26	2011-11-15	Ibis Biosciences, Inc.	Methods for identification of coronaviruses
US8158354B2 (en)	2003-05-13	2012-04-17	Ibis Biosciences, Inc.	Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US7964343B2 (en)	2003-05-13	2011-06-21	Ibis Biosciences, Inc.	Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US7205111B2 (en) *	2003-07-24	2007-04-17	Marshfield Clinic	Rapid identification of bacteria from positive blood cultures
US8546082B2 (en)	2003-09-11	2013-10-01	Ibis Biosciences, Inc.	Methods for identification of sepsis-causing bacteria
US8097416B2 (en)	2003-09-11	2012-01-17	Ibis Biosciences, Inc.	Methods for identification of sepsis-causing bacteria
US8242254B2 (en)	2003-09-11	2012-08-14	Ibis Biosciences, Inc.	Compositions for use in identification of bacteria
US8163895B2 (en)	2003-12-05	2012-04-24	Ibis Biosciences, Inc.	Compositions for use in identification of orthopoxviruses
US7666592B2 (en)	2004-02-18	2010-02-23	Ibis Biosciences, Inc.	Methods for concurrent identification and quantification of an unknown bioagent
ES2641832T3 (es)	2004-05-24	2017-11-14	Ibis Biosciences, Inc.	Espectrometría de masas con filtración de iones selectiva por establecimiento de umbrales digitales
US20050266411A1 (en)	2004-05-25	2005-12-01	Hofstadler Steven A	Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en)	2004-07-14	2010-10-12	Ibis Biosciences, Inc.	Methods for repairing degraded DNA
US7309589B2 (en) *	2004-08-20	2007-12-18	Vironix Llc	Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing
US8084207B2 (en)	2005-03-03	2011-12-27	Ibis Bioscience, Inc.	Compositions for use in identification of papillomavirus
US20060205040A1 (en)	2005-03-03	2006-09-14	Rangarajan Sampath	Compositions for use in identification of adventitious viruses
EP1904655A2 (fr)	2005-07-21	2008-04-02	Isis Pharmaceuticals, Inc.	Procedes pour l'identification et la quantification rapide de variants d'acide nucleique
EP2010679A2 (fr)	2006-04-06	2009-01-07	Ibis Biosciences, Inc.	Compositions pour l'identification de champignons
AU2007353877B2 (en)	2006-09-14	2012-07-19	Ibis Biosciences, Inc.	Targeted whole genome amplification method for identification of pathogens
JP5680304B2 (ja)	2007-02-23	2015-03-04	アイビスバイオサイエンシズインコーポレイティッド	迅速な法医学的ｄｎａ分析法
US9598724B2 (en)	2007-06-01	2017-03-21	Ibis Biosciences, Inc.	Methods and compositions for multiple displacement amplification of nucleic acids
EP2349549B1 (fr)	2008-09-16	2012-07-18	Ibis Biosciences, Inc.	Cartouches de mélange, postes de mélange et kits, et système associé
WO2010033627A2 (fr)	2008-09-16	2010-03-25	Ibis Biosciences, Inc.	Unités de traitement d'échantillons, systèmes et procédés associés
US8534447B2 (en)	2008-09-16	2013-09-17	Ibis Biosciences, Inc.	Microplate handling systems and related computer program products and methods
EP2396803A4 (fr)	2009-02-12	2016-10-26	Ibis Biosciences Inc	Ensembles sonde d'ionisation
US9719083B2 (en)	2009-03-08	2017-08-01	Ibis Biosciences, Inc.	Bioagent detection methods
US9393564B2 (en)	2009-03-30	2016-07-19	Ibis Biosciences, Inc.	Bioagent detection systems, devices, and methods
US8950604B2 (en)	2009-07-17	2015-02-10	Ibis Biosciences, Inc.	Lift and mount apparatus
EP2454000A4 (fr)	2009-07-17	2016-08-10	Ibis Biosciences Inc	Systèmes pour l'identification d'un bioagent
WO2011014811A1 (fr)	2009-07-31	2011-02-03	Ibis Biosciences, Inc.	Amorces de capture et supports solides liés à une séquence de capture pour tests diagnostiques moléculaires
EP3098325A1 (fr)	2009-08-06	2016-11-30	Ibis Biosciences, Inc.	Compositions de base déterminée sans masse pour la détection d'acide nucléique
EP2957641B1 (fr)	2009-10-15	2017-05-17	Ibis Biosciences, Inc.	Amplification de déplacement multiple
WO2011048227A1 (fr) *	2009-10-22	2011-04-28	Biotools Biotechnological & Medical Laboratories, S.A.	Composition, méthode et trousse pour la détection de bactéries par séquençage
US9758840B2 (en)	2010-03-14	2017-09-12	Ibis Biosciences, Inc.	Parasite detection via endosymbiont detection
JP5399312B2 (ja) *	2010-04-26	2014-01-29	日東電工株式会社	粘着シート
WO2016164407A2 (fr)	2015-04-07	2016-10-13	Polyskope Labs	Détection d'un ou de plusieurs agents pathogènes
WO2020190795A1 (fr) *	2019-03-15	2020-09-24	Polyskope Labs	Bouillon d'enrichissement sélectif pour la détection d'un ou plusieurs agents pathogènes

Citations (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1990001560A1 (fr) *	1988-07-29	1990-02-22	David John Evans	Sonde d'adn bacterienne
WO1990015157A1 (fr) *	1989-05-31	1990-12-13	Gene-Trak Systems	Sondes d'acides nucleiques eubacteriennes universelles et procede
EP0479117A1 (fr) *	1990-10-05	1992-04-08	F. Hoffmann-La Roche Ag	Méthodes et réactifs pour l'identification bactérienne
WO1993005179A1 (fr) *	1991-08-29	1993-03-18	THE UNITED STATES OF AMERICA, represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES:National Institutes of Health	Procede de discrimination et d'identification d'alleles dans des loci complexes

1993
- 1993-11-11 NL NL9301957A patent/NL9301957A/nl not_active Application Discontinuation
1994
- 1994-11-11 WO PCT/NL1994/000283 patent/WO1995013396A2/fr not_active Ceased

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1990001560A1 (fr) *	1988-07-29	1990-02-22	David John Evans	Sonde d'adn bacterienne
WO1990015157A1 (fr) *	1989-05-31	1990-12-13	Gene-Trak Systems	Sondes d'acides nucleiques eubacteriennes universelles et procede
EP0479117A1 (fr) *	1990-10-05	1992-04-08	F. Hoffmann-La Roche Ag	Méthodes et réactifs pour l'identification bactérienne
WO1993005179A1 (fr) *	1991-08-29	1993-03-18	THE UNITED STATES OF AMERICA, represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES:National Institutes of Health	Procede de discrimination et d'identification d'alleles dans des loci complexes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
G. SARKAR ET AL.: "Screening for mutations by RNA single-strand conformation polymorphism (rSSCP): comparison with DNA-SSCP", NUCL. ACIDS RES., vol. 20, no. 4, 25 February 1992 (1992-02-25), IRL PRESS, OXFORD, ENGLAND;, pages 871 - 878 *
K. CHEN ET AL.: "Broad range DNA probes for detecting and amplifying eubacterial nucleic acids", FEMS MICROBIOLGY LETTERS, vol. 57, no. 1, January 1989 (1989-01-01), ELSEVIER, AMSTERDAM, NL;, pages 19 - 24 *

Also Published As

Publication number	Publication date
WO1995013396A2 (fr)	1995-05-18
WO1995013396A3 (fr)	1995-06-08

Legal Events

Date	Code	Title	Description
1995-06-01	A1B	A search report has been drawn up
1996-06-03	BV	The patent application has lapsed

Publication	Publication Date	Title
US6699670B2 (en)	2004-03-02	Quantitative assay for the simultaneous detection and speciation of bacterial infections
Stender et al.	2002	PNA for rapid microbiology
Wolcott	1992	Advances in nucleic acid-based detection methods
US5298392A (en)	1994-03-29	Process for detection of water-borne microbial pathogens and indicators of human fecal contamination in water samples and kits therefor
WO1995013396A2 (fr)	1995-05-18	Procede d'identification de micro-organismes, et dispositifs pour sa mise en oeuvre
JP4176146B2 (ja)	2008-11-05	微生物検査室における日常的診断用の臨床検体からの通常の細菌病原体および抗生物質耐性遺伝子を迅速に検出および同定するための特異的および普遍的プローブおよび増幅プライマー
JP4481491B2 (ja)	2010-06-16	核酸の検出方法
JP3070529B2 (ja)	2000-07-31	ミコバクテリウム・カンサシイの種特異的検出
US10550438B2 (en)	2020-02-04	Methods and compositions for detecting bacterial nucleic acid
EP0630971A2 (fr)	1994-12-28	Procédé, réactifs et kits de tests pour détection de neisseria gonorrhoea
EP0709467A2 (fr)	1996-05-01	Compositions et procédés pour la détection de chlamydia trachomatis
Tomioka et al.	2005	A multiplex polymerase chain reaction microarray assay to detect bioterror pathogens in blood
JP2011510630A (ja)	2011-04-07	試料中に存在する目的ヌクレオチド配列を検出するための核酸プローブの使用
US20240117447A1 (en)	2024-04-11	Polynucleotides for the amplification and detection of neisseria gonorrhoeae
JPH08510386A (ja)	1996-11-05	ポリメラーゼ鎖反応によるサルモネラ菌の同定
US7879581B2 (en)	2011-02-01	Nucleic acid amplification and detection of mycobacterium species
AU8955998A (en)	1999-05-27	Assay for Chlamydia trachomatis by amplification and detection of Chlamydia trachomatis nucleic acid
AU781192B2 (en)	2005-05-12	Nucleic acid amplification and detection of mycobacterium species
EP2748320B1 (fr)	2018-06-13	Compositions et procédés d'hybridation d'acides nucléiques
US9593384B2 (en)	2017-03-14	Metronidazole resistance in trichomonas vaginalis and single nucleotide polymorphisms
Knight	2000	Molecular genetic methods for detection and identification of viable but nonculturable microorganisms
WO2005083114A1 (fr)	2005-09-09	Procede, trousse et systeme pour une meilleure pcr nichee
EP1252331B1 (fr)	2005-12-07	Procedes et compositions pour la detection d'especes du complexe micobacterium avium
JPH05184399A (ja)	1993-07-27	ジアルジン遺伝子含有ジアルジアの検出方法
JP2552787B2 (ja)	1996-11-13	結核菌の特異的検出法