NO20003531L - Fast faseseleksjon av differensielt uttrykte gener - Google Patents

Fast faseseleksjon av differensielt uttrykte gener

Info

Publication number
NO20003531L
NO20003531L NO20003531A NO20003531A NO20003531L NO 20003531 L NO20003531 L NO 20003531L NO 20003531 A NO20003531 A NO 20003531A NO 20003531 A NO20003531 A NO 20003531A NO 20003531 L NO20003531 L NO 20003531L
Authority
NO
Norway
Prior art keywords
microparticles
differentially expressed
expressed genes
sequencing
facs
Prior art date
Application number
NO20003531A
Other languages
English (en)
Other versions
NO20003531D0 (no
Inventor
Glen Albrecht
Sydney Brenner
Robert Dubridge
Original Assignee
Lynx Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lynx Therapeutics Inc filed Critical Lynx Therapeutics Inc
Publication of NO20003531D0 publication Critical patent/NO20003531D0/no
Publication of NO20003531L publication Critical patent/NO20003531L/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Oscillators With Electromechanical Resonators (AREA)

Abstract

Foreliggende oppfinnelse tilveiebringer en fremgangsmåte og materialer for å undersøke og isolere differensiert uttrykte gener. Ved fremgangsmåten ifølge foreliggende oppfinnelse blir forskjellige merkede populasjoner av DNAer fra kilder som skal sammenlignes kompeptivt hybridisert med referanse-DNA klonet på fast fasebærere, f.eks. mikropartikler, for å tilveiebringe et differensiert ekspresjonsbibliotek som de foretrukne utførelsesformer, kan bli manipulert ved hjelp av fluoresens-aktivert cellesortering (FACS). Undersøkelse av den relative signalintensiteten av forskjellige fluoresensmerker på mikropartiklene muliggjør kvantitativ analyse av ekspresjonsnivåer i forhold til referanse-DNA. Oppfinnelsen tilveiebringer også en fremgangsmåte for å identifisere å isolere sjeldne gener. Populasjoner av mikropartikler som har relative signalintensiteter av interesse kan bli isolert ved hjelp av FACS og de bundede DNAer identifisert ved sekvensering, slik som ved parallell signatursekvensiering (MPSS) i stort omfang, eller med vanlig DNA-sekvensfremgangsmåter.
NO20003531A 1998-01-09 2000-07-07 Fast faseseleksjon av differensielt uttrykte gener NO20003531L (no)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US522298A 1998-01-09 1998-01-09
US09/130,546 US6265163B1 (en) 1998-01-09 1998-08-06 Solid phase selection of differentially expressed genes
PCT/US1999/000666 WO1999035293A2 (en) 1998-01-09 1999-01-08 Solid phase selection of differentially expressed genes

Publications (2)

Publication Number Publication Date
NO20003531D0 NO20003531D0 (no) 2000-07-07
NO20003531L true NO20003531L (no) 2000-09-05

Family

ID=26674091

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20003531A NO20003531L (no) 1998-01-09 2000-07-07 Fast faseseleksjon av differensielt uttrykte gener

Country Status (10)

Country Link
US (2) US6265163B1 (no)
EP (1) EP1054999B1 (no)
KR (1) KR100433782B1 (no)
AT (1) ATE322556T1 (no)
AU (1) AU754929B2 (no)
CA (1) CA2317695A1 (no)
DE (1) DE69930729D1 (no)
NO (1) NO20003531L (no)
PL (1) PL342114A1 (no)
WO (1) WO1999035293A2 (no)

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Publication number Publication date
WO1999035293A3 (en) 1999-09-30
KR100433782B1 (ko) 2004-06-04
EP1054999A2 (en) 2000-11-29
NO20003531D0 (no) 2000-07-07
PL342114A1 (en) 2001-05-21
AU754929B2 (en) 2002-11-28
DE69930729D1 (de) 2006-05-18
ATE322556T1 (de) 2006-04-15
AU2113999A (en) 1999-07-26
WO1999035293A2 (en) 1999-07-15
US6511802B1 (en) 2003-01-28
US6265163B1 (en) 2001-07-24
KR20010033992A (ko) 2001-04-25
EP1054999B1 (en) 2006-04-05
CA2317695A1 (en) 1999-07-15

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