NO20005428L - Varmelabil uracil-DNA-glykosylase, samt anvendelse derav - Google Patents

Varmelabil uracil-DNA-glykosylase, samt anvendelse derav

Info

Publication number
NO20005428L
NO20005428L NO20005428A NO20005428A NO20005428L NO 20005428 L NO20005428 L NO 20005428L NO 20005428 A NO20005428 A NO 20005428A NO 20005428 A NO20005428 A NO 20005428A NO 20005428 L NO20005428 L NO 20005428L
Authority
NO
Norway
Prior art keywords
heat
gene
dna glycosylase
enzyme
uracil dna
Prior art date
Application number
NO20005428A
Other languages
English (en)
Other versions
NO20005428D0 (no
NO314091B1 (no
Inventor
Olav Lanes
Nils Peder Willasen
Per Henrik Guddal
Dag Rune Gjellesvik
Original Assignee
Biotec Asa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from NO20000163A external-priority patent/NO20000163L/no
Publication of NO20005428D0 publication Critical patent/NO20005428D0/no
Priority to NO20005428A priority Critical patent/NO314091B1/no
Application filed by Biotec Asa filed Critical Biotec Asa
Priority to EP01900799A priority patent/EP1246908B1/en
Priority to US09/758,017 priority patent/US7037703B2/en
Priority to DE60110893T priority patent/DE60110893T2/de
Priority to JP2001551197A priority patent/JP4808352B2/ja
Priority to AT01900799T priority patent/ATE295878T1/de
Priority to PCT/NO2001/000008 priority patent/WO2001051623A1/en
Priority to AU25596/01A priority patent/AU784783B2/en
Publication of NO20005428L publication Critical patent/NO20005428L/no
Publication of NO314091B1 publication Critical patent/NO314091B1/no

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/02Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2) hydrolysing N-glycosyl compounds (3.2.2)
    • C12Y302/02027Uracil-DNA glycosylase (3.2.2.27)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2497Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing N- glycosyl compounds (3.2.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
NO20005428A 2000-01-12 2000-10-27 Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet NO314091B1 (no)

Priority Applications (8)

Application Number Priority Date Filing Date Title
NO20005428A NO314091B1 (no) 2000-01-12 2000-10-27 Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet
AU25596/01A AU784783B2 (en) 2000-01-12 2001-01-10 Cod uracil-DNA glycosylase, gene coding therefore, recombinant DNA containing said gene or operative parts thereof, a method for preparing said protein and the use of said protein or said operative parts thereof in monitoring or controlling PCR
EP01900799A EP1246908B1 (en) 2000-01-12 2001-01-10 Cod uracil-dna glycosylase, gene coding therefore, recombinant dna containing said gene or operative parts thereof, a method for preparing said protein and the use of said protein or said operative parts thereof in monitoring or controlling pcr
PCT/NO2001/000008 WO2001051623A1 (en) 2000-01-12 2001-01-10 Cod uracil-dna glycosylase, gene coding therefore, recombinant dna containing said gene or operative parts thereof, a method for preparing said protein and the use of said protein or said operative parts thereof in monitoring or controlling pcr
US09/758,017 US7037703B2 (en) 2000-01-12 2001-01-10 Cod uracil-dna glycosylase, gene coding therefore, recombinant dna containing said gene or operative parts thereof, a method for preparing said protein and the use of said protein or said operative parts thereof in monitoring or controlling pcr
DE60110893T DE60110893T2 (de) 2000-01-12 2001-01-10 Kabeljau-spezifische urazil-dna glykosilase, dafür kodierendes gen, selbiges gen beinhaltende, rekombinante dna oder operative teile davon, ein verfahren zur herstellung des proteins und die anwendung des proteins oder der operativen teile zur überwachung und kontrolle der pcr
JP2001551197A JP4808352B2 (ja) 2000-01-12 2001-01-10 タラウラシル−dnaグリコシラーゼ、これをコードする遺伝子、該遺伝子またはその機能性部分を含有する組換えdna、該タンパク質の調製法と、pcrの追跡もしくは調節における該タンパク質またはその機能性部分の使用
AT01900799T ATE295878T1 (de) 2000-01-12 2001-01-10 Kabeljau-spezifische urazil-dna glykosilase, dafür kodierendes gen, selbiges gen beinhaltende, rekombinante dna oder operative teile davon, ein verfahren zur herstellung des proteins und die anwendung des protein oder der operativen teile zur überwachung und kontrolle der pcr

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
NO20000163A NO20000163L (no) 2000-01-12 2000-01-12 Varmelabil uracil-DNA-glykosylase samt anvendelse derav
NO20005428A NO314091B1 (no) 2000-01-12 2000-10-27 Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet

Publications (3)

Publication Number Publication Date
NO20005428D0 NO20005428D0 (no) 2000-10-27
NO20005428L true NO20005428L (no) 2001-07-13
NO314091B1 NO314091B1 (no) 2003-01-27

Family

ID=26649192

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20005428A NO314091B1 (no) 2000-01-12 2000-10-27 Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet

Country Status (8)

Country Link
US (1) US7037703B2 (no)
EP (1) EP1246908B1 (no)
JP (1) JP4808352B2 (no)
AT (1) ATE295878T1 (no)
AU (1) AU784783B2 (no)
DE (1) DE60110893T2 (no)
NO (1) NO314091B1 (no)
WO (1) WO2001051623A1 (no)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004535765A (ja) 2000-12-07 2004-12-02 カイロン コーポレイション 前立腺癌においてアップレギュレートされた内因性レトロウイルス
DE60322044D1 (de) 2002-02-21 2008-08-21 Asm Scient Inc Rekombinase-polymerase-amplifikation
US7399590B2 (en) * 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US8518694B2 (en) 2002-06-13 2013-08-27 Novartis Vaccines And Diagnostics, Inc. Nucleic acid vector comprising a promoter and a sequence encoding a polypeptide from the endogenous retrovirus PCAV
JP5027126B2 (ja) 2005-07-25 2012-09-19 アリーア サン ディエゴ, インコーポレイテッド リコンビナーゼポリメラーゼ増幅を多重化するための方法
JP2009535053A (ja) 2006-05-04 2009-10-01 エーエスエム サイエンティフィック, インコーポレイテッド レコンビナーゼポリメラーゼ増幅
KR100963014B1 (ko) 2007-10-18 2010-06-09 성균관대학교산학협력단 바실러스 스피시스 нj171 균주 유래의 신규한 저온성우라실-dna 글리코실라제의 제조방법 및 중합효소연쇄반응에서의 이용
JP5847076B2 (ja) * 2009-05-20 2016-01-20 バイオサイト インコーポレイテッド Dnaグリコシラーゼ/リアーゼおよびapエンドヌクレアーゼ基質
EP3360974B1 (en) 2009-06-05 2025-04-09 Abbott Diagnostics Scarborough, Inc. Recombinase polymerase amplification reagents
US8563298B2 (en) 2010-10-22 2013-10-22 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
WO2012054639A2 (en) 2010-10-22 2012-04-26 T2 Biosystems, Inc. Nmr systems and methods for the rapid detection of analytes
US8809021B2 (en) 2011-04-07 2014-08-19 Alere San Diego Inc. Monitoring recombinase polymerase amplification mixtures
GB201611469D0 (en) * 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
CN107828758A (zh) * 2017-11-13 2018-03-23 江苏众红生物工程创药研究院有限公司 重组尿嘧啶‑dna糖苷酶及其编码基因、制备方法和应用
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
CN112342231A (zh) * 2020-10-30 2021-02-09 厦门大学 一种不耐热ung融合蛋白的重组载体及表达纯化方法
WO2022232087A1 (en) * 2021-04-30 2022-11-03 Nutcracker Therapeutics, Inc. METHODS FOR PERFORMING IN VITRO TRANSCRIPTION USING dUTP
EP4569132A2 (en) * 2022-09-15 2025-06-18 Cheng-Yao Chen Method, kit and system for end labeling of nucleic acids

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19545320A1 (de) * 1995-12-05 1997-06-12 Boehringer Mannheim Gmbh Thermolabile Uracil-DNA-Glykosylase, Verfahren zu deren Herstellung und Verwendung zur Entfernung von Uracil aus DNA
GB9600384D0 (en) * 1996-01-09 1996-03-13 Nyfotek As Dna glycosylases

Also Published As

Publication number Publication date
EP1246908A1 (en) 2002-10-09
ATE295878T1 (de) 2005-06-15
EP1246908B1 (en) 2005-05-18
DE60110893T2 (de) 2006-04-27
US20020155573A1 (en) 2002-10-24
DE60110893D1 (de) 2005-06-23
WO2001051623A1 (en) 2001-07-19
NO20005428D0 (no) 2000-10-27
AU2559601A (en) 2001-07-24
NO314091B1 (no) 2003-01-27
JP2003519490A (ja) 2003-06-24
AU784783B2 (en) 2006-06-15
JP4808352B2 (ja) 2011-11-02
US7037703B2 (en) 2006-05-02

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