NO20061659L - Fremgangsmate for fremstilling av plasmid av hoy farmasoytisk kvalitet - Google Patents

Fremgangsmate for fremstilling av plasmid av hoy farmasoytisk kvalitet

Info

Publication number
NO20061659L
NO20061659L NO20061659A NO20061659A NO20061659L NO 20061659 L NO20061659 L NO 20061659L NO 20061659 A NO20061659 A NO 20061659A NO 20061659 A NO20061659 A NO 20061659A NO 20061659 L NO20061659 L NO 20061659L
Authority
NO
Norway
Prior art keywords
order
purification steps
pharmaceutical quality
pdna
continuous
Prior art date
Application number
NO20061659A
Other languages
English (en)
Inventor
Michel Couder
Francis Blanche
Nicolas Maestrali
David Gaillac
Thierry Guillemin
Original Assignee
Centelion
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centelion filed Critical Centelion
Publication of NO20061659L publication Critical patent/NO20061659L/no

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0091—Purification or manufacturing processes for gene therapy compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Manufacturing & Machinery (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicinal Preparation (AREA)

Abstract

Denne oppfinnelsen tilveiebringer en fremgangsmåte for den kontinuerlige alkaliske lysisen av en bakteriell suspensjon for å feste pDNA. Den tilveiebringer videre eventuelle ytterligere rensetrinn som inkluderer lysatfiltrering, anionebytterkromatografi, tredobbel affinitetskromatografi og hydrofob interaksjonskromatografi. Disse eventuelle rensetrinnene kan bli kombinert med den kontinuerlige lysisen for å produsere et sterkt renset pDNA produkt vesentlig fritt for gDNA, RNA, protein, endotoksin og andre kontaminanter.
NO20061659A 2003-09-17 2006-04-11 Fremgangsmate for fremstilling av plasmid av hoy farmasoytisk kvalitet NO20061659L (no)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US50346403P 2003-09-17 2003-09-17
US56300804P 2004-04-19 2004-04-19
PCT/EP2004/011437 WO2005026331A2 (en) 2003-09-17 2004-09-17 Method of preparation of pharmaceutically grade plasmid dna

Publications (1)

Publication Number Publication Date
NO20061659L true NO20061659L (no) 2006-06-13

Family

ID=42827706

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20061659A NO20061659L (no) 2003-09-17 2006-04-11 Fremgangsmate for fremstilling av plasmid av hoy farmasoytisk kvalitet

Country Status (17)

Country Link
US (1) US20070111221A1 (no)
EP (1) EP1664277B1 (no)
JP (1) JP2007505613A (no)
KR (1) KR101094032B1 (no)
CN (1) CN1882682B (no)
AT (1) ATE532853T1 (no)
AU (2) AU2004272748C1 (no)
BR (1) BRPI0413907A (no)
CA (1) CA2536931A1 (no)
EA (1) EA009447B1 (no)
ES (1) ES2376173T3 (no)
IL (1) IL174147A0 (no)
MX (1) MXPA06003004A (no)
NO (1) NO20061659L (no)
NZ (1) NZ545449A (no)
PT (1) PT1664277E (no)
WO (1) WO2005026331A2 (no)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0509490A (pt) * 2004-04-19 2007-09-11 Centelion método de purificação de plasmìdeo dna
DK1817423T3 (en) * 2004-11-30 2015-01-26 Merial Ltd Mixing units for chemical light ring of cells
WO2008019964A1 (de) * 2006-08-15 2008-02-21 Basf Se Verfahren zur isolierung von proteinen aus produktionszellen
WO2008045505A2 (en) 2006-10-10 2008-04-17 Xenomics, Inc. Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media
ES2375314T3 (es) 2007-02-28 2012-02-28 Lipoxen Technologies Limited Reducción de endotoxina en ácidos polisiálicos.
US20090004716A1 (en) * 2007-05-23 2009-01-01 Vgx Pharmaceuticlas, Inc. Compositions comprising high concentration of biologically active molecules and processes for preparing the same
US9017966B2 (en) 2007-05-23 2015-04-28 Nature Technology Corporation E. coli plasmid DNA production
US20080299621A1 (en) * 2007-05-31 2008-12-04 Ge Healthcare Uk Limited Miniprep system for simple and rapid plasmid dna extraction
KR101290322B1 (ko) * 2008-04-09 2013-07-26 주식회사 바이로메드 플라스미드 ｄｎａ의 발현 증강용 동결건조 ｄｎａ 제제
WO2011057199A1 (en) * 2009-11-06 2011-05-12 Adenios, Inc. Compositions for treating cns disorders
RU2488406C1 (ru) * 2012-02-16 2013-07-27 Общество с ограниченной ответственностью "НТфарма" Фармацевтическая композиция для терапии острых токсических состояний
RU2489168C1 (ru) * 2012-02-28 2013-08-10 Общество с ограниченной ответственностью "НТфарма" Фармацевтическая композиция, продуцирующая антиоксидантный, антимикробный, антитоксический белок - лактоферрин человека, способ ее получения и способ терапии
EP2682168A1 (en) 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
CN104099321A (zh) * 2014-07-10 2014-10-15 广州市军科泰特医药科技有限公司 抗肺癌T-VISA-BikDD质粒的制备方法
SG11201705312PA (en) * 2014-12-29 2017-07-28 Bonac Corp Composition containing nucleic acid molecule stably
CN106884011B (zh) * 2015-12-16 2020-11-17 固安鼎泰海规生物科技有限公司 一种规模化质粒纯化的联合液相层析分离方法
JP2019514394A (ja) 2016-05-06 2019-06-06 マックス−プランク−ゲゼルシャフト・ツア・フェルデルング・デア・ヴィッセンシャフテン・エー・ファオ プラグフローチューブ型バイオリアクター、それを含むシステム、およびウイルスの調製のための方法
MX2020012910A (es) * 2018-05-31 2021-05-27 Vgxi Inc Aparato de espiral de lisis y usos del mismo para el aislamiento y purificacion de polinucleotidos.
EP4127163A1 (en) 2020-03-31 2023-02-08 Richter-Helm Biologics GmbH & Co. KG Methods for producing plasmid dna
CN111394427B (zh) * 2020-04-22 2023-04-11 宁波市博坤生物科技有限公司 一种含泥土检材中微量dna的提取方法
EP3906982B2 (de) 2020-05-08 2025-08-13 AXAGARIUS GmbH & Co. KG Verfahren zur plasmidreinigung unter gleichzeitiger abreicherung von endotoxinen
JP2021074018A (ja) * 2021-02-12 2021-05-20 マックス−プランク−ゲゼルシャフト・ツア・フェルデルング・デア・ヴィッセンシャフテン・エー・ファオ プラグフローチューブ型バイオリアクター、それを含むシステム、およびウイルスの調製のための方法
CN115404232A (zh) * 2021-05-10 2022-11-29 艾棣维欣(苏州)生物制品有限公司 用于提取细菌中质粒dna的方法
CN115404154A (zh) * 2021-05-10 2022-11-29 艾棣维欣(苏州)生物制品有限公司 用于提取细菌中质粒dna的装置
JP2024519895A (ja) * 2021-05-18 2024-05-21 賽斯尓▲チン▼生物技術（上海）有限公司 細胞を修飾する方法
CN113332869B (zh) * 2021-06-23 2022-12-23 苏州沃美生物有限公司 混合装置和方法以及重组大肠杆菌质粒提取方法
JP2025534175A (ja) * 2022-10-19 2025-10-14 アベック，インコーポレイテッド 細胞溶解及びプラスミド単離システム
CN117187069B (zh) * 2023-09-18 2024-04-05 苏州依科赛生物科技股份有限公司 一种碱裂解工艺的大肠杆菌hcp检测方法
WO2025157970A1 (en) 2024-01-26 2025-07-31 Richter Biologics Gmbh & Co. Kg Large scale methods for producing plasmid dna
WO2025166642A1 (zh) * 2024-02-07 2025-08-14 首都医科大学 高生物活性质粒dna及其制备和存储、疫苗、试剂盒及其应用
WO2025252480A1 (en) 2024-06-07 2025-12-11 F. Hoffmann-La Roche Ag Method for purifying plasmid dna
JP2026006022A (ja) * 2024-06-28 2026-01-16 キヤノン株式会社 細胞処理方法、及び細胞処理装置

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2692764A (en) * 1951-01-19 1954-10-26 Howe Baker Corp Mixing apparatus
JPS5321458A (en) * 1976-08-11 1978-02-27 Hitachi Ltd Device for mixing fluids
US4737349A (en) * 1985-11-01 1988-04-12 Ciba-Geigy Corporation Tubular reactor for heterogeneous reactions
US5096818A (en) * 1990-06-04 1992-03-17 Autogen Instruments, Inc. Nucleic acid separation method
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
US6197553B1 (en) * 1994-07-15 2001-03-06 Merck & Co., Inc. Method for large scale plasmid purification
US5837529A (en) * 1994-10-17 1998-11-17 Genzyme Corporation Method for lysing cells
FR2728264B1 (fr) * 1994-12-16 1997-01-31 Rhone Poulenc Rorer Sa Purification d'adn par formation de triple helice avec un oligonucleotide immobilise
US5840254A (en) * 1995-06-02 1998-11-24 Cdc Technologies, Inc. Apparatus for mixing fluids for analysis
CA2212446C (en) * 1995-12-21 2010-09-21 Genzyme Corporation Method for lysing cells
FR2746412B1 (fr) * 1996-03-21 1998-06-12 Rhone Poulenc Rorer Sa Purification d'adn plasmidique de qualite pharmaceutique
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) * 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US5721120A (en) * 1996-10-02 1998-02-24 Merck & Co., Inc. Method of disrupting cultured cells using an impinging jet device
FR2773818B1 (fr) * 1998-01-21 2000-02-18 Pasteur Merieux Serums Vacc Procede et dispositif de lyse de bacteries
GB2338236B (en) * 1998-06-13 2003-04-09 Aea Technology Plc Microbiological cell processing
US6664049B1 (en) * 1999-01-20 2003-12-16 Aventis Pasteur S.A. Method and device for cell lysis
CA2375215C (en) * 1999-05-28 2012-11-20 Bio Science Contract Production Corporation Methods of dna purification and purified dna
EP1224326A1 (en) * 1999-10-02 2002-07-24 Bioneer Corporation; Automatic dna purification apparatus
JP2004509335A (ja) * 2000-09-18 2004-03-25 ユニバーシティ　オブ　ワシントン 複数のフローストリーム間の流体界面の回転操作のための微小流体デバイス
JP2005538717A (ja) * 2002-09-13 2005-12-22 ヴァレンティス，インコーポレイテッド 核酸のプレパラティブスケールの精製のための装置および方法
EP1578763B1 (en) * 2002-12-23 2009-01-07 Vical Incorporated Process for purification of plasmid dna

Also Published As

Publication number Publication date
BRPI0413907A (pt) 2006-10-24
EA009447B1 (ru) 2007-12-28
WO2005026331A3 (en) 2005-11-17
EA200600579A1 (ru) 2006-10-27
MXPA06003004A (es) 2006-06-23
AU2004272748C1 (en) 2010-07-08
JP2007505613A (ja) 2007-03-15
KR101094032B1 (ko) 2011-12-19
KR20060086941A (ko) 2006-08-01
IL174147A0 (en) 2006-08-01
AU2004272748B2 (en) 2009-12-03
CN1882682A (zh) 2006-12-20
ES2376173T3 (es) 2012-03-09
ATE532853T1 (de) 2011-11-15
CN1882682B (zh) 2010-06-16
HK1096994A1 (en) 2007-06-15
EP1664277B1 (en) 2011-11-09
NZ545449A (en) 2009-07-31
EP1664277A2 (en) 2006-06-07
PT1664277E (pt) 2012-01-17
WO2005026331A2 (en) 2005-03-24
AU2004272748A1 (en) 2005-03-24
AU2010200801A1 (en) 2010-03-25
CA2536931A1 (en) 2005-03-24
US20070111221A1 (en) 2007-05-17

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