NO314091B1 - Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet - Google Patents

Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet Download PDF

Info

Publication number: NO314091B1
Authority: NO; Norway
Prior art keywords: dna; uracil; enzyme; ung; activity
Prior art date: 2000-01-12

Application number

NO20005428A

Other languages

English (en)

Norwegian (no)

Other versions

NO20005428L (no

NO20005428D0 (no

Inventor

Olav Lanes

Nils Peder Willasen

Per Henrik Guddal

Dag Rune Gjellesvik

Original Assignee

Biotec Pharmacon Asa

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-01-12

Filing date

2000-10-27

Publication date

2003-01-27

2000-01-12 Priority claimed from NO20000163A external-priority patent/NO20000163L/no

2000-10-27 Publication of NO20005428D0 publication Critical patent/NO20005428D0/no

2000-10-27 Priority to NO20005428A priority Critical patent/NO314091B1/no

2000-10-27 Application filed by Biotec Pharmacon Asa filed Critical Biotec Pharmacon Asa

2001-01-10 Priority to AU25596/01A priority patent/AU784783B2/en

2001-01-10 Priority to DE60110893T priority patent/DE60110893T2/de

2001-01-10 Priority to PCT/NO2001/000008 priority patent/WO2001051623A1/en

2001-01-10 Priority to AT01900799T priority patent/ATE295878T1/de

2001-01-10 Priority to US09/758,017 priority patent/US7037703B2/en

2001-01-10 Priority to JP2001551197A priority patent/JP4808352B2/ja

2001-01-10 Priority to EP01900799A priority patent/EP1246908B1/de

2001-07-13 Publication of NO20005428L publication Critical patent/NO20005428L/no

2003-01-27 Publication of NO314091B1 publication Critical patent/NO314091B1/no

Links

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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/02—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2) hydrolysing N-glycosyl compounds (3.2.2)
- C12Y302/02027—Uracil-DNA glycosylase (3.2.2.27)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2497—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing N- glycosyl compounds (3.2.2)

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Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Genetics & Genomics (AREA)
Bioinformatics & Cheminformatics (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Enzymes And Modification Thereof (AREA)
Saccharide Compounds (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Bakery Products And Manufacturing Methods Therefor (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

NO20005428A 2000-01-12 2000-10-27 Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet NO314091B1 (no)

Priority Applications (8)

Application Number	Priority Date	Filing Date	Title
NO20005428A NO314091B1 (no)	2000-01-12	2000-10-27	Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet
EP01900799A EP1246908B1 (de)	2000-01-12	2001-01-10	Kabeljau-spezifische urazil-dna glykosilase, dafür kodierendes gen, selbiges gen beinhaltende, rekombinante dna oder operative teile davon, ein verfahren zur herstellung des proteins und die anwendung des protein oder der operativen teile zur überwachung und kontrolle der pcr
AU25596/01A AU784783B2 (en)	2000-01-12	2001-01-10	Cod uracil-DNA glycosylase, gene coding therefore, recombinant DNA containing said gene or operative parts thereof, a method for preparing said protein and the use of said protein or said operative parts thereof in monitoring or controlling PCR
JP2001551197A JP4808352B2 (ja)	2000-01-12	2001-01-10	タラウラシル−ｄｎａグリコシラーゼ、これをコードする遺伝子、該遺伝子またはその機能性部分を含有する組換えｄｎａ、該タンパク質の調製法と、ｐｃｒの追跡もしくは調節における該タンパク質またはその機能性部分の使用
DE60110893T DE60110893T2 (de)	2000-01-12	2001-01-10	Kabeljau-spezifische urazil-dna glykosilase, dafür kodierendes gen, selbiges gen beinhaltende, rekombinante dna oder operative teile davon, ein verfahren zur herstellung des proteins und die anwendung des proteins oder der operativen teile zur überwachung und kontrolle der pcr
PCT/NO2001/000008 WO2001051623A1 (en)	2000-01-12	2001-01-10	Cod uracil-dna glycosylase, gene coding therefore, recombinant dna containing said gene or operative parts thereof, a method for preparing said protein and the use of said protein or said operative parts thereof in monitoring or controlling pcr
AT01900799T ATE295878T1 (de)	2000-01-12	2001-01-10	Kabeljau-spezifische urazil-dna glykosilase, dafür kodierendes gen, selbiges gen beinhaltende, rekombinante dna oder operative teile davon, ein verfahren zur herstellung des proteins und die anwendung des protein oder der operativen teile zur überwachung und kontrolle der pcr
US09/758,017 US7037703B2 (en)	2000-01-12	2001-01-10	Cod uracil-dna glycosylase, gene coding therefore, recombinant dna containing said gene or operative parts thereof, a method for preparing said protein and the use of said protein or said operative parts thereof in monitoring or controlling pcr

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
NO20000163A NO20000163L (no)	2000-01-12	2000-01-12	Varmelabil uracil-DNA-glykosylase samt anvendelse derav
NO20005428A NO314091B1 (no)	2000-01-12	2000-10-27	Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet

Publications (3)

Publication Number	Publication Date
NO20005428D0 NO20005428D0 (no)	2000-10-27
NO20005428L NO20005428L (no)	2001-07-13
NO314091B1 true NO314091B1 (no)	2003-01-27

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NO20005428A NO314091B1 (no)	2000-01-12	2000-10-27	Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet

Country Status (8)

Country	Link
US (1)	US7037703B2 (de)
EP (1)	EP1246908B1 (de)
JP (1)	JP4808352B2 (de)
AT (1)	ATE295878T1 (de)
AU (1)	AU784783B2 (de)
DE (1)	DE60110893T2 (de)
NO (1)	NO314091B1 (de)
WO (1)	WO2001051623A1 (de)

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US8030000B2 (en)	2002-02-21	2011-10-04	Alere San Diego, Inc.	Recombinase polymerase amplification
DK1499738T3 (da) *	2002-02-21	2008-11-17	Asm Scient Inc	Recombinase-polymerease-amplifikation
US8518694B2 (en)	2002-06-13	2013-08-27	Novartis Vaccines And Diagnostics, Inc.	Nucleic acid vector comprising a promoter and a sequence encoding a polypeptide from the endogenous retrovirus PCAV
EP2829615B1 (de)	2005-07-25	2018-05-09	Alere San Diego, Inc.	Kit zur Multiplexierung einer Rekombinase-Polymerase-Verstärkung
EP2029782B1 (de)	2006-05-04	2014-11-26	Alere San Diego, Inc.	Rekombinase-polymerase-verstärkung
KR100963014B1 (ko)	2007-10-18	2010-06-09	성균관대학교산학협력단	바실러스 스피시스 нｊ171 균주 유래의 신규한 저온성우라실-ｄｎａ 글리코실라제의 제조방법 및 중합효소연쇄반응에서의 이용
JP5847076B2 (ja) *	2009-05-20	2016-01-20	バイオサイトインコーポレイテッド	Ｄｎａグリコシラーゼ／リアーゼおよびａｐエンドヌクレアーゼ基質
WO2010141940A1 (en)	2009-06-05	2010-12-09	Alere San Diego, Inc.	Recombinase polymerase amplification reagents and kits
US8563298B2 (en)	2010-10-22	2013-10-22	T2 Biosystems, Inc.	NMR systems and methods for the rapid detection of analytes
EP3974831A1 (de)	2010-10-22	2022-03-30	T2 Biosystems, Inc.	Verfahren zum nachweis eines bakteriums in einer vollblutprobe
EP3978620A1 (de)	2011-04-07	2022-04-06	Abbott Diagnostics Scarborough, Inc.	Überwachung von rekombinase-polymerase-verstärkungsmischungen
GB201611469D0 (en) *	2016-06-30	2016-08-17	Lumiradx Tech Ltd	Improvements in or relating to nucleic acid amplification processes
CN107828758A (zh) *	2017-11-13	2018-03-23	江苏众红生物工程创药研究院有限公司	重组尿嘧啶‑dna糖苷酶及其编码基因、制备方法和应用
GB2569965A (en)	2018-01-04	2019-07-10	Lumiradx Uk Ltd	Improvements in or relating to amplification of nucleic acids
CN112342231A (zh) *	2020-10-30	2021-02-09	厦门大学	一种不耐热ung融合蛋白的重组载体及表达纯化方法
WO2022232087A1 (en) *	2021-04-30	2022-11-03	Nutcracker Therapeutics, Inc.	METHODS FOR PERFORMING IN VITRO TRANSCRIPTION USING dUTP
TWI872707B (zh) *	2022-09-15	2025-02-11	呈堯陳	用於核酸末端標記的方法、套組和系統

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DE19545320A1 (de)	1995-12-05	1997-06-12	Boehringer Mannheim Gmbh	Thermolabile Uracil-DNA-Glykosylase, Verfahren zu deren Herstellung und Verwendung zur Entfernung von Uracil aus DNA
GB9600384D0 (en)	1996-01-09	1996-03-13	Nyfotek As	Dna glycosylases

2000
- 2000-10-27 NO NO20005428A patent/NO314091B1/no not_active IP Right Cessation
2001
- 2001-01-10 AU AU25596/01A patent/AU784783B2/en not_active Expired
- 2001-01-10 US US09/758,017 patent/US7037703B2/en not_active Expired - Lifetime
- 2001-01-10 DE DE60110893T patent/DE60110893T2/de not_active Expired - Lifetime
- 2001-01-10 WO PCT/NO2001/000008 patent/WO2001051623A1/en not_active Ceased
- 2001-01-10 JP JP2001551197A patent/JP4808352B2/ja not_active Expired - Lifetime
- 2001-01-10 AT AT01900799T patent/ATE295878T1/de not_active IP Right Cessation
- 2001-01-10 EP EP01900799A patent/EP1246908B1/de not_active Expired - Lifetime

Also Published As

Publication number	Publication date
ATE295878T1 (de)	2005-06-15
US20020155573A1 (en)	2002-10-24
JP4808352B2 (ja)	2011-11-02
JP2003519490A (ja)	2003-06-24
NO20005428L (no)	2001-07-13
DE60110893T2 (de)	2006-04-27
AU2559601A (en)	2001-07-24
NO20005428D0 (no)	2000-10-27
WO2001051623A1 (en)	2001-07-19
DE60110893D1 (de)	2005-06-23
US7037703B2 (en)	2006-05-02
EP1246908A1 (de)	2002-10-09
AU784783B2 (en)	2006-06-15
EP1246908B1 (de)	2005-05-18

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2020-11-09	MK1K	Patent expired

Publication	Publication Date	Title
EP1246908B1 (de)	2005-05-18	Kabeljau-spezifische urazil-dna glykosilase, dafür kodierendes gen, selbiges gen beinhaltende, rekombinante dna oder operative teile davon, ein verfahren zur herstellung des proteins und die anwendung des protein oder der operativen teile zur überwachung und kontrolle der pcr
US10982247B2 (en)	2021-04-20	DNA polymerase mutants having enhanced template discrimination activity
Chohan et al.	2020	Heterologous gene expression and characterization of TK2246, a highly active and thermostable plant type l-asparaginase from Thermococcus kodakarensis
US8114653B2 (en)	2012-02-14	Thermostable Y-family polymerases and chimeras
Rohman et al.	2008	Effect of the disease‐causing mutations identified in human ribonuclease (RNase) H2 on the activities and stabilities of yeast RNase H2 and archaeal RNase HII
Lanes et al.	2000	Purification and characterization of a cold-adapted uracil-DNA glycosylase from Atlantic cod (Gadus morhua)
Zhang et al.	2022	Biochemical and functional characterization of a thermostable RecJ exonuclease from Thermococcus gammatolerans
Kriukiene et al.	2005	MnlI—the member of HNH subtype of Type IIS restriction endonucleases
Jiang et al.	2021	Biochemical characterization and mutational studies of a novel 3-methlyadenine DNA glycosylase II from the hyperthermophilic Thermococcus gammatolerans
Shi et al.	2019	Biochemical characterization and mutational studies of a thermostable uracil DNA glycosylase from the hyperthermophilic euryarchaeon Thermococcus barophilus Ch5
Cacciapuoti et al.	2005	A novel hyperthermostable 5′‐deoxy‐5′‐methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus
Nguyen et al.	2013	Crystal structure of metagenome‐derived LC9‐RNase H1 with atypical DEDN active site motif
US20090246756A1 (en)	2009-10-01	Thermostable polypeptide having polynucleotide kinase activity and/or phosphatase activity
Nguyen et al.	2013	Crystal structure of metagenome-derived LC11-RNase H1 in complex with RNA/DNA hybrid
Jaeger et al.	2000	Molecular cloning, sequencing, and expression of the heat-labile uracil-DNA glycosylase from a marine psychrophilic bacterium, strain BMTU3346
Jongruja et al.	2010	The N‐terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg2+‐dependent activity
Sandigursky et al.	2001	Characterization of the full length uracil-DNA glycosylase in the extreme thermophile Thermotoga maritima
Chen et al.	2025	Structure and function of the uracil DNA glycosylases from hyperthermophiles: Elucidating DNA uracil repair mechanisms: A review
Lin et al.	2022	Biochemical characterization and mutational analysis of a novel flap endonuclease 1 from Thermococcus barophilus Ch5
Wu et al.	2023	Biochemical characterization and mutational studies of endonuclease Q from the hyperthermophilic euryarchaeon Thermococcus gammatolerans
Kim et al.	2009	Properties of cold-active uracil-DNA glycosylase from Photobacterium aplysiae GMD509, and its PCR application for carryover contamination control
JPWO2006095769A1 (ja)	2008-08-14	低温性微生物由来エンドヌクレアーゼ
Chon et al.	2006	Identification of RNase HII from psychrotrophic bacterium, Shewanella sp. SIB1 as a high‐activity type RNase H
Permanasari et al.	2013	Role of N‐terminal extension of B acillus stearothermophilus RN ase H 2 and C‐terminal extension of T hermotoga maritima RN ase H 2
Back et al.	2006	A distinct TthMutY bifunctional glycosylase that hydrolyzes not only adenine but also thymine opposite 8-oxoguanine in the hyperthermophilic bacterium, Thermus thermophilus