NO742267L - - Google Patents

Info

Publication number: NO742267L
Authority: NO; Norway
Prior art keywords: microorganisms; sample; preparation; treatment; rhodamine
Prior art date: 1973-06-22

Application number

NO742267A

Other languages

English (en)

Norwegian (no)

Inventor

J Scholefield

Original Assignee

Univ Strathclyde

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1973-06-22

Filing date

1974-06-21

Publication date

1974-12-27

1974-06-21 Application filed by Univ Strathclyde filed Critical Univ Strathclyde

1974-12-27 Publication of NO742267L publication Critical patent/NO742267L/no

Links

238000000034 method Methods 0.000 claims description 54
244000005700 microbiome Species 0.000 claims description 47
238000002360 preparation method Methods 0.000 claims description 25
238000011282 treatment Methods 0.000 claims description 13
239000000975 dye Substances 0.000 claims description 7
239000007788 liquid Substances 0.000 claims description 6
QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 5
238000006243 chemical reaction Methods 0.000 claims description 5
BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 claims description 4
DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 claims description 3
DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 claims description 3
238000004040 coloring Methods 0.000 claims description 3
238000010438 heat treatment Methods 0.000 claims description 3
229960000907 methylthioninium chloride Drugs 0.000 claims description 3
239000000126 substance Substances 0.000 claims description 3
IOOMXAQUNPWDLL-UHFFFAOYSA-N 2-[6-(diethylamino)-3-(diethyliminiumyl)-3h-xanthen-9-yl]-5-sulfobenzene-1-sulfonate Chemical compound C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=C(S(O)(=O)=O)C=C1S([O-])(=O)=O IOOMXAQUNPWDLL-UHFFFAOYSA-N 0.000 claims description 2
YDIKCZBMBPOGFT-PWUSVEHZSA-N Malvidin 3-galactoside Chemical compound [Cl-].COC1=C(O)C(OC)=CC(C=2C(=CC=3C(O)=CC(O)=CC=3[O+]=2)O[C@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)=C1 YDIKCZBMBPOGFT-PWUSVEHZSA-N 0.000 claims description 2
PXUQTDZNOHRWLI-QOPOCTTISA-O Primulin Natural products O(C)c1c(O)c(OC)cc(-c2c(O[C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O3)cc3c(O)cc(O)cc3[o+]2)c1 PXUQTDZNOHRWLI-QOPOCTTISA-O 0.000 claims description 2
108010076830 Thionins Proteins 0.000 claims description 2
CPUMJJMOTBXNBQ-UHFFFAOYSA-N [6-amino-9-(2-ethoxycarbonylphenyl)-7-methylxanthen-3-ylidene]-dimethylazanium;chloride Chemical compound [Cl-].CCOC(=O)C1=CC=CC=C1C(C1=CC(C)=C(N)C=C1O1)=C2C1=CC(=[N+](C)C)C=C2 CPUMJJMOTBXNBQ-UHFFFAOYSA-N 0.000 claims description 2
PEJLNXHANOHNSU-UHFFFAOYSA-N acridine-3,6-diamine;10-methylacridin-10-ium-3,6-diamine;chloride Chemical compound [Cl-].C1=CC(N)=CC2=NC3=CC(N)=CC=C3C=C21.C1=C(N)C=C2[N+](C)=C(C=C(N)C=C3)C3=CC2=C1 PEJLNXHANOHNSU-UHFFFAOYSA-N 0.000 claims description 2
JPIYZTWMUGTEHX-UHFFFAOYSA-N auramine O free base Chemical compound C1=CC(N(C)C)=CC=C1C(=N)C1=CC=C(N(C)C)C=C1 JPIYZTWMUGTEHX-UHFFFAOYSA-N 0.000 claims description 2
SEACYXSIPDVVMV-UHFFFAOYSA-L eosin Y Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C([O-])=C(Br)C=C21 SEACYXSIPDVVMV-UHFFFAOYSA-L 0.000 claims description 2
230000032050 esterification Effects 0.000 claims description 2
238000005886 esterification reaction Methods 0.000 claims description 2
GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims description 2
230000007062 hydrolysis Effects 0.000 claims description 2
238000006460 hydrolysis reaction Methods 0.000 claims description 2
230000003647 oxidation Effects 0.000 claims description 2
238000007254 oxidation reaction Methods 0.000 claims description 2
PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 claims description 2
RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims 1
238000004090 dissolution Methods 0.000 claims 1
238000001704 evaporation Methods 0.000 claims 1
230000011987 methylation Effects 0.000 claims 1
238000007069 methylation reaction Methods 0.000 claims 1
239000000376 reactant Substances 0.000 claims 1
229940043267 rhodamine b Drugs 0.000 claims 1
ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 claims 1
210000004027 cell Anatomy 0.000 description 14
239000000243 solution Substances 0.000 description 13
239000000463 material Substances 0.000 description 8
241000894006 Bacteria Species 0.000 description 7
235000013305 food Nutrition 0.000 description 6
238000010186 staining Methods 0.000 description 6
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Substances CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 5
238000011109 contamination Methods 0.000 description 5
238000002474 experimental method Methods 0.000 description 5
238000011534 incubation Methods 0.000 description 5
RAHZWNYVWXNFOC-UHFFFAOYSA-N Sulphur dioxide Chemical compound O=S=O RAHZWNYVWXNFOC-UHFFFAOYSA-N 0.000 description 4
235000019441 ethanol Nutrition 0.000 description 4
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
239000011521 glass Substances 0.000 description 3
239000001963 growth medium Substances 0.000 description 3
238000004519 manufacturing process Methods 0.000 description 3
235000013372 meat Nutrition 0.000 description 3
230000000813 microbial effect Effects 0.000 description 3
238000012360 testing method Methods 0.000 description 3
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
240000004808 Saccharomyces cerevisiae Species 0.000 description 2
238000009835 boiling Methods 0.000 description 2
239000007853 buffer solution Substances 0.000 description 2
238000009826 distribution Methods 0.000 description 2
238000007654 immersion Methods 0.000 description 2
CXKWCBBOMKCUKX-UHFFFAOYSA-M methylene blue Chemical compound [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 CXKWCBBOMKCUKX-UHFFFAOYSA-M 0.000 description 2
239000008267 milk Substances 0.000 description 2
210000004080 milk Anatomy 0.000 description 2
235000013336 milk Nutrition 0.000 description 2
238000012986 modification Methods 0.000 description 2
230000004048 modification Effects 0.000 description 2
VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 2
FYSNRJHAOHDILO-UHFFFAOYSA-N thionyl chloride Chemical compound ClS(Cl)=O FYSNRJHAOHDILO-UHFFFAOYSA-N 0.000 description 2
238000005406 washing Methods 0.000 description 2
PVPBBTJXIKFICP-UHFFFAOYSA-N (7-aminophenothiazin-3-ylidene)azanium;chloride Chemical compound [Cl-].C1=CC(=[NH2+])C=C2SC3=CC(N)=CC=C3N=C21 PVPBBTJXIKFICP-UHFFFAOYSA-N 0.000 description 1
KKAJSJJFBSOMGS-UHFFFAOYSA-N 3,6-diamino-10-methylacridinium chloride Chemical compound [Cl-].C1=C(N)C=C2[N+](C)=C(C=C(N)C=C3)C3=CC2=C1 KKAJSJJFBSOMGS-UHFFFAOYSA-N 0.000 description 1
YXHKONLOYHBTNS-UHFFFAOYSA-N Diazomethane Chemical compound C=[N+]=[N-] YXHKONLOYHBTNS-UHFFFAOYSA-N 0.000 description 1
241000192125 Firmicutes Species 0.000 description 1
235000003805 Musa ABB Group Nutrition 0.000 description 1
240000008790 Musa x paradisiaca Species 0.000 description 1
GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
235000015266 Plantago major Nutrition 0.000 description 1
241000191967 Staphylococcus aureus Species 0.000 description 1
NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
229940023020 acriflavine Drugs 0.000 description 1
239000013543 active substance Substances 0.000 description 1
210000004666 bacterial spore Anatomy 0.000 description 1
230000000903 blocking effect Effects 0.000 description 1
239000000872 buffer Substances 0.000 description 1
WUKWITHWXAAZEY-UHFFFAOYSA-L calcium difluoride Chemical compound [F-].[F-].[Ca+2] WUKWITHWXAAZEY-UHFFFAOYSA-L 0.000 description 1
238000004364 calculation method Methods 0.000 description 1
239000003086 colorant Substances 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
238000001514 detection method Methods 0.000 description 1
SWXVUIWOUIDPGS-UHFFFAOYSA-N diacetone alcohol Natural products CC(=O)CC(C)(C)O SWXVUIWOUIDPGS-UHFFFAOYSA-N 0.000 description 1
238000003745 diagnosis Methods 0.000 description 1
239000003814 drug Substances 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
230000005284 excitation Effects 0.000 description 1
238000000855 fermentation Methods 0.000 description 1
230000004151 fermentation Effects 0.000 description 1
239000010436 fluorite Substances 0.000 description 1
XLSMFKSTNGKWQX-UHFFFAOYSA-N hydroxyacetone Chemical compound CC(=O)CO XLSMFKSTNGKWQX-UHFFFAOYSA-N 0.000 description 1
230000000873 masking effect Effects 0.000 description 1
QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
229910052751 metal Inorganic materials 0.000 description 1
239000002184 metal Substances 0.000 description 1
WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
230000002906 microbiologic effect Effects 0.000 description 1
238000000386 microscopy Methods 0.000 description 1
239000000203 mixture Substances 0.000 description 1
229910017604 nitric acid Inorganic materials 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
239000007800 oxidant agent Substances 0.000 description 1
KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 1
239000000825 pharmaceutical preparation Substances 0.000 description 1
229940127557 pharmaceutical product Drugs 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
239000002985 plastic film Substances 0.000 description 1
229920006255 plastic film Polymers 0.000 description 1
239000000843 powder Substances 0.000 description 1
239000002243 precursor Substances 0.000 description 1
238000003908 quality control method Methods 0.000 description 1
238000011002 quantification Methods 0.000 description 1
239000002994 raw material Substances 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
239000002904 solvent Substances 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
229910052717 sulfur Inorganic materials 0.000 description 1
239000011593 sulfur Substances 0.000 description 1
-1 sulfuric acid ester Chemical class 0.000 description 1
241001515965 unidentified phage Species 0.000 description 1
235000013311 vegetables Nutrition 0.000 description 1
230000035899 viability Effects 0.000 description 1
230000003313 weakening effect Effects 0.000 description 1
238000005303 weighing Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/04—Dairy products

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Physics & Mathematics (AREA)
General Health & Medical Sciences (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
Immunology (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Toxicology (AREA)
Genetics & Genomics (AREA)
Biomedical Technology (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Pyrane Compounds (AREA)

NO742267A 1973-06-22 1974-06-21 NO742267L (fr)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
GB2967473		1973-06-22

Publications (1)

Publication Number	Publication Date
NO742267L true NO742267L (fr)	1974-12-27

Family

ID=10295293

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO742267A NO742267L (fr)	1973-06-22	1974-06-21

Country Status (8)

Country	Link
JP (1)	JPS5076282A (fr)
BE (1)	BE816657A (fr)
DE (1)	DE2429380A1 (fr)
DK (1)	DK334774A (fr)
FR (1)	FR2234368A1 (fr)
NL (1)	NL7408337A (fr)
NO (1)	NO742267L (fr)
SE (1)	SE7408105L (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB1503828A (en) *	1976-06-22	1978-03-15	Univ Strathclyde	Method of enumerating bacteria
CA1112987A (fr) *	1978-09-25	1981-11-24	Joseph L. Melnick	Coloration et analyse des bacteries
DE19503434A1 (de) *	1995-02-03	1996-08-08	Beiersdorf Ag	Verfahren zur Bestimmung der Adhäsion von Mikroorganismen und der antiadhäsiven Wirkung von Stoffen
CN103091145B (zh) *	2013-01-30	2015-04-29	中国人民解放军军事医学科学院生物工程研究所	微生物的染色方法

1974
- 1974-06-19 SE SE7408105A patent/SE7408105L/xx unknown
- 1974-06-19 DE DE2429380A patent/DE2429380A1/de not_active Withdrawn
- 1974-06-20 NL NL7408337A patent/NL7408337A/xx not_active Application Discontinuation
- 1974-06-21 DK DK334774A patent/DK334774A/da unknown
- 1974-06-21 FR FR7421574A patent/FR2234368A1/fr active Granted
- 1974-06-21 NO NO742267A patent/NO742267L/no unknown
- 1974-06-21 JP JP49070344A patent/JPS5076282A/ja active Pending
- 1974-06-21 BE BE2053700A patent/BE816657A/fr not_active IP Right Cessation

Also Published As

Publication number	Publication date
JPS5076282A (fr)	1975-06-21
SE7408105L (fr)	1974-12-23
DK334774A (fr)	1975-02-17
FR2234368A1 (en)	1975-01-17
BE816657A (fr)	1974-10-16
NL7408337A (fr)	1974-12-24
FR2234368B3 (fr)	1977-04-22
DE2429380A1 (de)	1975-01-23

Publication	Publication Date	Title
US4094745A (en)	1978-06-13	Method of staining microscopic organisms
Dubey et al.	2002	Practical Microbiology, 4/e
DE69127387T2 (de)	1998-01-02	Vorrichtung und Verfahren zum Nachweis von Mikroorganismen
US4665024A (en)	1987-05-12	Fluorescent gram stain
CN103175768B (zh)	2016-05-04	快速检测生物细胞死活状态的荧光染色试剂盒及其应用
Gunasekaran	2007	Laboratory manual in microbiology
CN104502317A (zh)	2015-04-08	用于在固体或半固体介质上表征微生物的方法
US5747277A (en)	1998-05-05	Process for detecting microorganisms
US4639421A (en)	1987-01-27	Fluorescent gram stain
JP2010213598A (ja)	2010-09-30	抗菌薬の微生物に対する有効性の検査方法
CA1089339A (fr)	1980-11-11	Methode de teinture des micro-organismes
Betts et al.	1989	Rapid enumeration of viable micro‐organisms by staining and direct microscopy
NO742267L (fr)	1974-12-27
DE3429823A1 (de)	1986-02-27	Mittel und verfahren zum nachweis antimikrobiell wirksamer substanzen
DE102005006237A1 (de)	2006-08-24	Verfahren zur Bestimmung von Keimen
Sana et al.	2023	Fundamentals of Microbiology: A Laboratory Manual
JPH11146798A (ja)	1999-06-02	微生物細胞迅速識別法と微生物細胞用識別キット
JP2002010799A (ja)	2002-01-15	細菌検査培地
JP2004053570A (ja)	2004-02-19	微生物の検査方法
RU2005790C1 (ru)	1994-01-15	Способ выявления бактерий pasteurella multocida
RU2081917C1 (ru)	1997-06-20	Способ идентификации бруцелл
JP2000210099A (ja)	2000-08-02	微生物の計測方法
JPH11346759A (ja)	1999-12-21	火落菌数の測定法
RU2179580C1 (ru)	2002-02-20	Способ дифференцирования грамположительных и грамотрицательных бактерий и простейших рода trichomonas
Ceccato-Antonini	2022	Microbiological Techniques and Methods for the Assessment of Microbial Contamination