NO813759L - Plasmid, fremgangsmaate for fremstilling av et slikt produkt, samt anvendelse derav - Google Patents

Plasmid, fremgangsmaate for fremstilling av et slikt produkt, samt anvendelse derav

Info

Publication number: NO813759L
Authority: NO; Norway
Prior art keywords: site; hind iii; pst; pwt; ecori
Prior art date: 1980-11-10

Application number

NO813759A

Other languages

English (en)

Norwegian (no)

Inventor

William Charlse Albert Tacon

John Spencer Emtage

Andrew George Stewart

Norman Henry Carey

Michael Houghton

Original Assignee

Searle & Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1980-11-10

Filing date

1981-11-06

Publication date

1982-05-11

1981-11-06 Application filed by Searle & Co filed Critical Searle & Co

1982-05-11 Publication of NO813759L publication Critical patent/NO813759L/no

Links

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SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
230000007812 deficiency Effects 0.000 description 1
239000007857 degradation product Substances 0.000 description 1
230000000593 degrading effect Effects 0.000 description 1
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239000003599 detergent Substances 0.000 description 1
230000002900 effect on cell Effects 0.000 description 1
239000012636 effector Substances 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
229960005542 ethidium bromide Drugs 0.000 description 1
238000001400 expression cloning Methods 0.000 description 1
239000011536 extraction buffer Substances 0.000 description 1
239000000706 filtrate Substances 0.000 description 1
235000003642 hunger Nutrition 0.000 description 1
230000002209 hydrophobic effect Effects 0.000 description 1
230000002401 inhibitory effect Effects 0.000 description 1
238000011081 inoculation Methods 0.000 description 1
230000003993 interaction Effects 0.000 description 1
238000011031 large-scale manufacturing process Methods 0.000 description 1
239000007788 liquid Substances 0.000 description 1
238000011068 loading method Methods 0.000 description 1
229910052943 magnesium sulfate Inorganic materials 0.000 description 1
235000019341 magnesium sulphate Nutrition 0.000 description 1
238000013507 mapping Methods 0.000 description 1
239000012528 membrane Substances 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000012986 modification Methods 0.000 description 1
229940056360 penicillin g Drugs 0.000 description 1
LQRJAEQXMSMEDP-XCHBZYMASA-N peptide a Chemical compound N([C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](C)C(=O)NCCCC[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)C(\NC(=O)[C@@H](CCCCN)NC(=O)CNC(C)=O)=C/C=1C=CC=CC=1)C(N)=O)C(=O)C(\NC(=O)[C@@H](CCCCN)NC(=O)CNC(C)=O)=C\C1=CC=CC=C1 LQRJAEQXMSMEDP-XCHBZYMASA-N 0.000 description 1
229920002223 polystyrene Polymers 0.000 description 1
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238000003127 radioimmunoassay Methods 0.000 description 1
230000003134 recirculating effect Effects 0.000 description 1
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238000011160 research Methods 0.000 description 1
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210000002966 serum Anatomy 0.000 description 1
IFGCUJZIWBUILZ-UHFFFAOYSA-N sodium 2-[[2-[[hydroxy-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyphosphoryl]amino]-4-methylpentanoyl]amino]-3-(1H-indol-3-yl)propanoic acid Chemical compound [Na+].C=1NC2=CC=CC=C2C=1CC(C(O)=O)NC(=O)C(CC(C)C)NP(O)(=O)OC1OC(C)C(O)C(O)C1O IFGCUJZIWBUILZ-UHFFFAOYSA-N 0.000 description 1
159000000000 sodium salts Chemical class 0.000 description 1
239000007790 solid phase Substances 0.000 description 1
238000000527 sonication Methods 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
235000019157 thiamine Nutrition 0.000 description 1
239000011721 thiamine Substances 0.000 description 1
DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
229940113082 thymine Drugs 0.000 description 1
210000001519 tissue Anatomy 0.000 description 1
230000005026 transcription initiation Effects 0.000 description 1
230000002463 transducing effect Effects 0.000 description 1
238000000844 transformation Methods 0.000 description 1
239000001226 triphosphate Substances 0.000 description 1
235000011178 triphosphate Nutrition 0.000 description 1
125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 1
101150044170 trpE gene Proteins 0.000 description 1
239000012588 trypsin Substances 0.000 description 1
239000012137 tryptone Substances 0.000 description 1
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239000011691 vitamin B1 Substances 0.000 description 1
235000010374 vitamin B1 Nutrition 0.000 description 1
239000012138 yeast extract Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Virology (AREA)
Gastroenterology & Hepatology (AREA)
Medicinal Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Saccharide Compounds (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

NO813759A 1980-11-10 1981-11-06 Plasmid, fremgangsmaate for fremstilling av et slikt produkt, samt anvendelse derav NO813759L (no)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
GB8036080		1980-11-10

Publications (1)

Publication Number	Publication Date
NO813759L true NO813759L (no)	1982-05-11

Family

ID=10517203

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO813759A NO813759L (no)	1980-11-10	1981-11-06	Plasmid, fremgangsmaate for fremstilling av et slikt produkt, samt anvendelse derav

Country Status (8)

Country	Link
EP (1)	EP0052002A3 (de)
JP (1)	JPS57163396A (de)
AU (1)	AU7731281A (de)
DK (1)	DK489481A (de)
ES (1)	ES506994A0 (de)
NO (1)	NO813759L (de)
PT (1)	PT73945B (de)
ZA (1)	ZA817735B (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
ZA811368B (en) *	1980-03-24	1982-04-28	Genentech Inc	Bacterial polypedtide expression employing tryptophan promoter-operator
US5174993A (en) *	1981-12-24	1992-12-29	Health Research Inc.	Recombinant avipox virus and immunological use thereof
US7767449B1 (en)	1981-12-24	2010-08-03	Health Research Incorporated	Methods using modified vaccinia virus
US5833975A (en) *	1989-03-08	1998-11-10	Virogenetics Corporation	Canarypox virus expressing cytokine and/or tumor-associated antigen DNA sequence
US4582800A (en) *	1982-07-12	1986-04-15	Hoffmann-La Roche Inc.	Novel vectors and method for controlling interferon expression
EP0113372A1 (de) *	1982-07-13	1984-07-18	Celltech Limited	Vektor
EP0121569B1 (de) *	1982-10-08	1990-08-22	Kyowa Hakko Kogyo Co., Ltd.	Neuer vektor
US4559300A (en) *	1983-01-18	1985-12-17	Eli Lilly And Company	Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces
EP0147178B1 (de) *	1983-12-23	1991-08-14	Pfizer Inc.	Expressionsplasmide für die Herstellung eines heterologen Proteins in Bakterien
US4935370A (en) *	1983-12-23	1990-06-19	Pfizer Inc.	Expression plasmids for improved production of heterologous protein in bacteria
US4663281A (en) *	1984-03-22	1987-05-05	Mass Institute Of Technology	Enhanced production of proteinaceous materials in eucaryotic cells
CA1338664C (en) *	1985-04-10	1996-10-22	Cetus Oncology Corporation	Recombinant hosts
AU582288B2 (en) *	1986-03-07	1989-03-16	Damon Biotech Inc.	Vector and method for achieving high level expression in eukaryotic cells
DE10399032I1 (de) *	1987-08-28	2004-01-29	Health Research Inc	Rekombinante Viren.
US7049102B1 (en)	1989-09-22	2006-05-23	Board Of Trustees Of Leland Stanford University	Multi-gene expression profile

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB2068970B (en) *	1980-02-06	1983-06-22	Searle & Co	Recombinant dna technique for the preparation of a protein resembling human interferon
ZA811368B (en) *	1980-03-24	1982-04-28	Genentech Inc	Bacterial polypedtide expression employing tryptophan promoter-operator
CH657141A5 (de) *	1980-07-01	1986-08-15	Hoffmann La Roche	Dns-sequenzen, rekombinante expressionsvektoren zur mikrobiellen herstellung von human-leukozyten-interferonen und transformierte mikroorganismen.
NZ198445A (en) *	1980-09-25	1984-05-31	Genentech Inc	Production of human fibroblast interferon by recombinant dna technology
ATE22327T1 (de) *	1980-12-12	1986-10-15	Unilever Nv	Dna-sequenzen fuer verschiedene allele formen des reifen thaumatins, diese sequenzen enthaltende rekombinante plasmide, verfahren zur herstellung dieser plasmide, diese rekombinante plasmide enthaltende bakterielle kulturen und verfahren zur herstellung von reifen thaumatin.

1981
- 1981-11-04 DK DK489481A patent/DK489481A/da not_active Application Discontinuation
- 1981-11-06 NO NO813759A patent/NO813759L/no unknown
- 1981-11-09 ZA ZA817735A patent/ZA817735B/xx unknown
- 1981-11-09 PT PT73945A patent/PT73945B/pt unknown
- 1981-11-09 EP EP81305308A patent/EP0052002A3/de not_active Withdrawn
- 1981-11-09 AU AU77312/81A patent/AU7731281A/en not_active Abandoned
- 1981-11-10 ES ES506994A patent/ES506994A0/es active Granted
- 1981-11-10 JP JP56180262A patent/JPS57163396A/ja active Pending

Also Published As

Publication number	Publication date
ES8304199A1 (es)	1983-02-16
JPS57163396A (en)	1982-10-07
EP0052002A2 (de)	1982-05-19
ZA817735B (en)	1982-10-27
ES506994A0 (es)	1983-02-16
AU7731281A (en)	1982-05-20
PT73945A (en)	1981-12-01
EP0052002A3 (de)	1983-03-16
PT73945B (en)	1983-04-18
DK489481A (da)	1982-05-11

Publication	Publication Date	Title
JP2528849B2 (ja)	1996-08-28	置換されたプロモ−タ−を使用する宿主による異種起原蛋白質の増強された分泌
Chang et al.	1987	High-level secretion of human growth hormone by Escherichia coli
NO813759L (no)	1982-05-11	Plasmid, fremgangsmaate for fremstilling av et slikt produkt, samt anvendelse derav
DK173092B1 (da)	2000-01-10	Rekombinant plasmid, fremgangsmåde til fremstilling af plasmidet, bakterie indeholdende plasmidet, samt fremgangsmåde til f
AU625554B2 (en)	1992-07-16	CKS method of protein synthesis
Kaster et al.	1983	Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencing
CA2122135C (en)	1999-12-21	Recombinant core-streptavidin
NO830780L (no)	1983-09-09	Uttrykking, behandling og sekresjon av heterologt protein av gjaer
JPH0776596A (ja)	1995-03-20	ペプチド、融合ペプチド、発現ベクター及び組換えタンパク質の製造方法
NZ199391A (en)	1985-12-13	Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin
NO158880B (no)	1988-08-01	Fremgangsmaate for fremstilling av et spesifikt pattedyrhormon.
IE63126B1 (en)	1995-03-22	Improved vectors and methods for making such vectors and for expressing cloned genes
JPH09135691A (ja)	1997-05-27	牛のプレ成長および成長ホルモンの製造方法
NO783724L (no)	1979-05-09	Syntetisk dna samt fremgangsmaate til dets fremstilling
AU607973B2 (en)	1991-03-21	Process for production of physiologically active peptide containing cysteine residue
WO1991017245A1 (en)	1991-11-14	Ubiquitin-specific protease
NO175871B (de)	1994-09-12
AP80A (en)	1990-04-16	Expression of HIV binding proteins.
US5401642A (en)	1995-03-28	Vectors and methods for making such vectors and for expressing cloned genes
WO1991009952A1 (en)	1991-07-11	Lipoprotein signal peptide fused to antigenic polypeptides
NZ207925A (en)	1988-05-30	Yeast expression vehicle consisting of a yeast promoter and signal peptide encoding region linked to a heterologus peptide coding region; expression and culture
EP1714972B1 (de)	2011-10-19	Verfahren zur Herstellung eines Peptids mit einem pI grösser als 8 oder kleiner als 5
US4828988A (en)	1989-05-09	Hybrid polypeptides comprising somatocrinine and alpha1 -antitrypsin, method for their production from bacterial clones and use thereof for the production of somatocrinine
WO1999053033A1 (en)	1999-10-21	Methods and compositions for high yield production of eukaryotic proteins
Maclntyre et al.	1991	Requirement of the SecB chaperone for export of a non-secretory polypeptide in Escherichia coli