NO842720L - In vivo markering av polynukleotidsekvenser - Google Patents

In vivo markering av polynukleotidsekvenser

Info

Publication number: NO842720L
Authority: NO; Norway
Prior art keywords: residue; polynucleotide sequence; host; signaling; dna
Prior art date: 1983-07-05

Application number

NO842720A

Other languages

English (en)

Norwegian (no)

Inventor

Jannis Stavrianopoulos

Normann E Kelker

Heuy-Lang Yang

Original Assignee

Enzo Biochem Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-07-05

Filing date

1984-07-04

Publication date

1985-01-07

1984-07-04 Application filed by Enzo Biochem Inc filed Critical Enzo Biochem Inc

1985-01-07 Publication of NO842720L publication Critical patent/NO842720L/no

Links

108091033319 polynucleotide Proteins 0.000 title claims abstract description 119
102000040430 polynucleotide Human genes 0.000 title claims abstract description 119
239000002157 polynucleotide Substances 0.000 title claims abstract description 119
238000001727 in vivo Methods 0.000 title claims abstract description 20
238000000034 method Methods 0.000 claims abstract description 81
238000001514 detection method Methods 0.000 claims abstract description 52
108091028043 Nucleic acid sequence Proteins 0.000 claims description 77
239000003550 marker Substances 0.000 claims description 66
230000011664 signaling Effects 0.000 claims description 59
108020004414 DNA Proteins 0.000 claims description 56
239000012491 analyte Substances 0.000 claims description 32
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 30
238000009396 hybridization Methods 0.000 claims description 27
125000003729 nucleotide group Chemical group 0.000 claims description 27
108010062580 Concanavalin A Proteins 0.000 claims description 26
102000004190 Enzymes Human genes 0.000 claims description 23
108090000790 Enzymes Proteins 0.000 claims description 23
229940088598 enzyme Drugs 0.000 claims description 23
239000002773 nucleotide Substances 0.000 claims description 21
229960002685 biotin Drugs 0.000 claims description 15
235000020958 biotin Nutrition 0.000 claims description 15
239000011616 biotin Substances 0.000 claims description 15
230000002285 radioactive effect Effects 0.000 claims description 15
239000002585 base Substances 0.000 claims description 14
230000000295 complement effect Effects 0.000 claims description 13
150000001875 compounds Chemical class 0.000 claims description 12
239000002777 nucleoside Substances 0.000 claims description 12
150000003833 nucleoside derivatives Chemical class 0.000 claims description 10
230000010076 replication Effects 0.000 claims description 10
IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims description 9
238000010367 cloning Methods 0.000 claims description 9
108010001336 Horseradish Peroxidase Proteins 0.000 claims description 7
108090001090 Lectins Proteins 0.000 claims description 7
102000004856 Lectins Human genes 0.000 claims description 7
239000002523 lectin Substances 0.000 claims description 7
239000002243 precursor Substances 0.000 claims description 7
108010051457 Acid Phosphatase Proteins 0.000 claims description 6
102000013563 Acid Phosphatase Human genes 0.000 claims description 6
108010015776 Glucose oxidase Proteins 0.000 claims description 6
239000004366 Glucose oxidase Substances 0.000 claims description 6
239000003153 chemical reaction reagent Substances 0.000 claims description 6
229940116332 glucose oxidase Drugs 0.000 claims description 6
235000019420 glucose oxidase Nutrition 0.000 claims description 6
241000588724 Escherichia coli Species 0.000 claims description 5
108090000623 proteins and genes Proteins 0.000 claims description 5
RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims description 5
GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 claims description 4
108020004774 Alkaline Phosphatase Proteins 0.000 claims description 4
102000002260 Alkaline Phosphatase Human genes 0.000 claims description 4
DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 claims description 4
241000701533 Escherichia virus T4 Species 0.000 claims description 4
239000000427 antigen Substances 0.000 claims description 4
108091007433 antigens Proteins 0.000 claims description 4
102000036639 antigens Human genes 0.000 claims description 4
IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 claims description 4
230000012010 growth Effects 0.000 claims description 4
229920001184 polypeptide Polymers 0.000 claims description 4
108090000765 processed proteins & peptides Proteins 0.000 claims description 4
102000004196 processed proteins & peptides Human genes 0.000 claims description 4
229940104230 thymidine Drugs 0.000 claims description 4
241001515965 unidentified phage Species 0.000 claims description 4
LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical compound CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 claims description 3
-1 antibodies Proteins 0.000 claims description 3
102000005936 beta-Galactosidase Human genes 0.000 claims description 3
108010005774 beta-Galactosidase Proteins 0.000 claims description 3
125000001295 dansyl group Chemical group [H]C1=C([H])C(N(C([H])([H])[H])C([H])([H])[H])=C2C([H])=C([H])C([H])=C(C2=C1[H])S(*)(=O)=O 0.000 claims description 3
GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims description 3
102000004169 proteins and genes Human genes 0.000 claims description 3
PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 claims description 3
229940113082 thymine Drugs 0.000 claims description 2
241000702194 Bacillus virus SPO1 Species 0.000 claims 1
241000701542 Enterobacteria phage T2 Species 0.000 claims 1
241000701536 Enterobacteria phage T6 Species 0.000 claims 1
241000589652 Xanthomonas oryzae Species 0.000 claims 1
239000000969 carrier Substances 0.000 claims 1
230000008569 process Effects 0.000 abstract description 7
238000002372 labelling Methods 0.000 abstract description 4
239000000523 sample Substances 0.000 description 67
WOVKYSAHUYNSMH-RRKCRQDMSA-N 5-bromodeoxyuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-RRKCRQDMSA-N 0.000 description 14
239000000243 solution Substances 0.000 description 14
ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 12
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
WOVKYSAHUYNSMH-UHFFFAOYSA-N BROMODEOXYURIDINE Natural products C1C(O)C(CO)OC1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-UHFFFAOYSA-N 0.000 description 11
229950004398 broxuridine Drugs 0.000 description 11
239000000203 mixture Substances 0.000 description 10
108091003079 Bovine Serum Albumin Proteins 0.000 description 9
229940098773 bovine serum albumin Drugs 0.000 description 9
108091060296 Glucosylated DNA Proteins 0.000 description 8
239000000872 buffer Substances 0.000 description 8
102000039446 nucleic acids Human genes 0.000 description 7
108020004707 nucleic acids Proteins 0.000 description 7
150000007523 nucleic acids Chemical class 0.000 description 7
108091008146 restriction endonucleases Proteins 0.000 description 7
239000003298 DNA probe Substances 0.000 description 6
238000006243 chemical reaction Methods 0.000 description 6
238000011534 incubation Methods 0.000 description 6
238000003780 insertion Methods 0.000 description 6
230000037431 insertion Effects 0.000 description 6
239000011780 sodium chloride Substances 0.000 description 6
102000053602 DNA Human genes 0.000 description 5
239000000020 Nitrocellulose Substances 0.000 description 5
108020004511 Recombinant DNA Proteins 0.000 description 5
210000004027 cell Anatomy 0.000 description 5
230000002255 enzymatic effect Effects 0.000 description 5
238000004519 manufacturing process Methods 0.000 description 5
229920001220 nitrocellulos Polymers 0.000 description 5
239000000126 substance Substances 0.000 description 5
208000026350 Inborn Genetic disease Diseases 0.000 description 4
230000015572 biosynthetic process Effects 0.000 description 4
208000016361 genetic disease Diseases 0.000 description 4
LEQAOMBKQFMDFZ-UHFFFAOYSA-N glyoxal Chemical compound O=CC=O LEQAOMBKQFMDFZ-UHFFFAOYSA-N 0.000 description 4
229910052739 hydrogen Inorganic materials 0.000 description 4
239000000047 product Substances 0.000 description 4
230000035945 sensitivity Effects 0.000 description 4
108090001008 Avidin Proteins 0.000 description 3
241000894006 Bacteria Species 0.000 description 3
241000233866 Fungi Species 0.000 description 3
229920005654 Sephadex Polymers 0.000 description 3
239000012507 Sephadex™ Substances 0.000 description 3
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 3
241000700605 Viruses Species 0.000 description 3
238000004458 analytical method Methods 0.000 description 3
230000006287 biotinylation Effects 0.000 description 3
238000007413 biotinylation Methods 0.000 description 3
238000003776 cleavage reaction Methods 0.000 description 3
230000002616 endonucleolytic effect Effects 0.000 description 3
125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 3
UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 3
239000001257 hydrogen Substances 0.000 description 3
238000005259 measurement Methods 0.000 description 3
238000007899 nucleic acid hybridization Methods 0.000 description 3
238000011160 research Methods 0.000 description 3
230000007017 scission Effects 0.000 description 3
239000000758 substrate Substances 0.000 description 3
238000003786 synthesis reaction Methods 0.000 description 3
YMXHPSHLTSZXKH-RVBZMBCESA-N (2,5-dioxopyrrolidin-1-yl) 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoate Chemical compound C([C@H]1[C@H]2NC(=O)N[C@H]2CS1)CCCC(=O)ON1C(=O)CCC1=O YMXHPSHLTSZXKH-RVBZMBCESA-N 0.000 description 2
108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
244000063299 Bacillus subtilis Species 0.000 description 2
235000014469 Bacillus subtilis Nutrition 0.000 description 2
241000195493 Cryptophyta Species 0.000 description 2
108020003215 DNA Probes Proteins 0.000 description 2
230000004543 DNA replication Effects 0.000 description 2
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
241000196324 Embryophyta Species 0.000 description 2
XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 2
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
229920001213 Polysorbate 20 Polymers 0.000 description 2
108020004682 Single-Stranded DNA Proteins 0.000 description 2
208000002903 Thalassemia Diseases 0.000 description 2
239000007983 Tris buffer Substances 0.000 description 2
ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
239000012472 biological sample Substances 0.000 description 2
238000005119 centrifugation Methods 0.000 description 2
239000002738 chelating agent Substances 0.000 description 2
238000007385 chemical modification Methods 0.000 description 2
OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
239000012149 elution buffer Substances 0.000 description 2
238000005516 engineering process Methods 0.000 description 2
238000001914 filtration Methods 0.000 description 2
230000014509 gene expression Effects 0.000 description 2
125000003147 glycosyl group Chemical group 0.000 description 2
229940015043 glyoxal Drugs 0.000 description 2
239000001963 growth medium Substances 0.000 description 2
238000000338 in vitro Methods 0.000 description 2
239000000463 material Substances 0.000 description 2
238000000691 measurement method Methods 0.000 description 2
239000002609 medium Substances 0.000 description 2
244000005700 microbiome Species 0.000 description 2
238000012986 modification Methods 0.000 description 2
230000004048 modification Effects 0.000 description 2
125000003835 nucleoside group Chemical group 0.000 description 2
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
230000007781 signaling event Effects 0.000 description 2
239000001632 sodium acetate Substances 0.000 description 2
235000017281 sodium acetate Nutrition 0.000 description 2
239000011550 stock solution Substances 0.000 description 2
238000012360 testing method Methods 0.000 description 2
210000001519 tissue Anatomy 0.000 description 2
239000001226 triphosphate Substances 0.000 description 2
235000011178 triphosphate Nutrition 0.000 description 2
125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 2
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
239000002023 wood Substances 0.000 description 2
YRCRRHNVYVFNTM-UHFFFAOYSA-N 1,1-dihydroxy-3-ethoxy-2-butanone Chemical compound CCOC(C)C(=O)C(O)O YRCRRHNVYVFNTM-UHFFFAOYSA-N 0.000 description 1
UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 1
MJEQLGCFPLHMNV-UHFFFAOYSA-N 4-amino-1-(hydroxymethyl)pyrimidin-2-one Chemical compound NC=1C=CN(CO)C(=O)N=1 MJEQLGCFPLHMNV-UHFFFAOYSA-N 0.000 description 1
PFJBDCNEHIXTLU-VPCXQMTMSA-N 4-amino-1-[(2r,3r,4s,5r)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]pyrimidin-2-one Chemical compound O=C1N=C(N)C=CN1[C@@]1(CO)[C@H](O)[C@H](O)[C@@H](CO)O1 PFJBDCNEHIXTLU-VPCXQMTMSA-N 0.000 description 1
ZATZIBWEALYTHN-UHFFFAOYSA-N 4-methyl-n-[(4-nitrophenyl)methyldiazenyl]aniline Chemical compound C1=CC(C)=CC=C1NN=NCC1=CC=C([N+]([O-])=O)C=C1 ZATZIBWEALYTHN-UHFFFAOYSA-N 0.000 description 1
AMIOJPURYLGJEG-UHFFFAOYSA-N 5-(4,5-dihydroxypentyl)uracil Chemical class OCC(O)CCCC1=CNC(=O)NC1=O AMIOJPURYLGJEG-UHFFFAOYSA-N 0.000 description 1
TVNSATFCUCQBGE-ZKWXMUAHSA-N 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanethioic s-acid Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)S)SC[C@@H]21 TVNSATFCUCQBGE-ZKWXMUAHSA-N 0.000 description 1
JDBGXEHEIRGOBU-UHFFFAOYSA-N 5-hydroxymethyluracil Chemical compound OCC1=CNC(=O)NC1=O JDBGXEHEIRGOBU-UHFFFAOYSA-N 0.000 description 1
RXGJTUSBYWCRBK-UHFFFAOYSA-M 5-methylphenazinium methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC=C2[N+](C)=C(C=CC=C3)C3=NC2=C1 RXGJTUSBYWCRBK-UHFFFAOYSA-M 0.000 description 1
102000007469 Actins Human genes 0.000 description 1
108010085238 Actins Proteins 0.000 description 1
229930024421 Adenine Natural products 0.000 description 1
GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
241000193830 Bacillus <bacterium> Species 0.000 description 1
108020000946 Bacterial DNA Proteins 0.000 description 1
208000035143 Bacterial infection Diseases 0.000 description 1
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 1
102000004594 DNA Polymerase I Human genes 0.000 description 1
108010017826 DNA Polymerase I Proteins 0.000 description 1
108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 1
102100033215 DNA nucleotidylexotransferase Human genes 0.000 description 1
AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
108010042407 Endonucleases Proteins 0.000 description 1
102000004533 Endonucleases Human genes 0.000 description 1
241000287828 Gallus gallus Species 0.000 description 1
241000282412 Homo Species 0.000 description 1
241000829100 Macaca mulatta polyomavirus 1 Species 0.000 description 1
FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
241000124008 Mammalia Species 0.000 description 1
PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
241001465754 Metazoa Species 0.000 description 1
238000000636 Northern blotting Methods 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
208000030852 Parasitic disease Diseases 0.000 description 1
241000589516 Pseudomonas Species 0.000 description 1
101710086015 RNA ligase Proteins 0.000 description 1
241000701835 Salmonella virus P22 Species 0.000 description 1
238000002105 Southern blotting Methods 0.000 description 1
108010090804 Streptavidin Proteins 0.000 description 1
241000187747 Streptomyces Species 0.000 description 1
ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical class CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 1
239000013504 Triton X-100 Substances 0.000 description 1
229920004890 Triton X-100 Polymers 0.000 description 1
HSCJRCZFDFQWRP-JZMIEXBBSA-N UDP-alpha-D-glucose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OP(O)(=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-JZMIEXBBSA-N 0.000 description 1
HSCJRCZFDFQWRP-UHFFFAOYSA-N Uridindiphosphoglukose Natural products OC1C(O)C(O)C(CO)OC1OP(O)(=O)OP(O)(=O)OCC1C(O)C(O)C(N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-UHFFFAOYSA-N 0.000 description 1
108020005202 Viral DNA Proteins 0.000 description 1
241000726445 Viroids Species 0.000 description 1
RPLKOJPSPZIYKF-PJKMHFRUSA-N [(2r,3s,5s)-5-(4-amino-2-oxopyrimidin-1-yl)-3-hydroxy-5-(hydroxymethyl)oxolan-2-yl]methyl dihydrogen phosphate Chemical class O=C1N=C(N)C=CN1[C@]1(CO)O[C@H](COP(O)(O)=O)[C@@H](O)C1 RPLKOJPSPZIYKF-PJKMHFRUSA-N 0.000 description 1
SXEHKFHPFVVDIR-UHFFFAOYSA-N [4-(4-hydrazinylphenyl)phenyl]hydrazine Chemical compound C1=CC(NN)=CC=C1C1=CC=C(NN)C=C1 SXEHKFHPFVVDIR-UHFFFAOYSA-N 0.000 description 1
239000002253 acid Substances 0.000 description 1
229960000643 adenine Drugs 0.000 description 1
230000032683 aging Effects 0.000 description 1
229910000147 aluminium phosphate Inorganic materials 0.000 description 1
230000003321 amplification Effects 0.000 description 1
230000001745 anti-biotin effect Effects 0.000 description 1
229910052786 argon Inorganic materials 0.000 description 1
208000022362 bacterial infectious disease Diseases 0.000 description 1
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
230000033228 biological regulation Effects 0.000 description 1
230000000903 blocking effect Effects 0.000 description 1
210000004369 blood Anatomy 0.000 description 1
239000008280 blood Substances 0.000 description 1
229910021538 borax Inorganic materials 0.000 description 1
239000006227 byproduct Substances 0.000 description 1
239000011575 calcium Substances 0.000 description 1
229910052791 calcium Inorganic materials 0.000 description 1
239000001110 calcium chloride Substances 0.000 description 1
235000011148 calcium chloride Nutrition 0.000 description 1
229910001628 calcium chloride Inorganic materials 0.000 description 1
230000009920 chelation Effects 0.000 description 1
238000001311 chemical methods and process Methods 0.000 description 1
BKHZIBWEHPHYAI-UHFFFAOYSA-N chloroform;3-methylbutan-1-ol Chemical compound ClC(Cl)Cl.CC(C)CCO BKHZIBWEHPHYAI-UHFFFAOYSA-N 0.000 description 1
MVWKRECLSFGFPK-UHFFFAOYSA-N chloroform;octan-1-ol Chemical compound ClC(Cl)Cl.CCCCCCCCO MVWKRECLSFGFPK-UHFFFAOYSA-N 0.000 description 1
230000002759 chromosomal effect Effects 0.000 description 1
210000000349 chromosome Anatomy 0.000 description 1
238000004440 column chromatography Methods 0.000 description 1
230000002860 competitive effect Effects 0.000 description 1
239000012141 concentrate Substances 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
238000012258 culturing Methods 0.000 description 1
UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 description 1
IERHLVCPSMICTF-XVFCMESISA-N cytidine 5'-monophosphate Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(O)=O)O1 IERHLVCPSMICTF-XVFCMESISA-N 0.000 description 1
IERHLVCPSMICTF-UHFFFAOYSA-N cytidine monophosphate Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(COP(O)(O)=O)O1 IERHLVCPSMICTF-UHFFFAOYSA-N 0.000 description 1
229940104302 cytosine Drugs 0.000 description 1
SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
230000007423 decrease Effects 0.000 description 1
238000011161 development Methods 0.000 description 1
230000018109 developmental process Effects 0.000 description 1
238000003745 diagnosis Methods 0.000 description 1
150000004985 diamines Chemical class 0.000 description 1
125000004989 dicarbonyl group Chemical group 0.000 description 1
239000000539 dimer Substances 0.000 description 1
238000005538 encapsulation Methods 0.000 description 1
238000001704 evaporation Methods 0.000 description 1
230000008020 evaporation Effects 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
238000000605 extraction Methods 0.000 description 1
210000003608 fece Anatomy 0.000 description 1
238000000855 fermentation Methods 0.000 description 1
230000004151 fermentation Effects 0.000 description 1
239000012530 fluid Substances 0.000 description 1
239000007850 fluorescent dye Substances 0.000 description 1
239000012634 fragment Substances 0.000 description 1
208000024386 fungal infectious disease Diseases 0.000 description 1
239000007789 gas Substances 0.000 description 1
239000011521 glass Substances 0.000 description 1
108091005608 glycosylated proteins Proteins 0.000 description 1
102000035122 glycosylated proteins Human genes 0.000 description 1
230000005484 gravity Effects 0.000 description 1
NAQMVNRVTILPCV-UHFFFAOYSA-N hexane-1,6-diamine Chemical compound NCCCCCCN NAQMVNRVTILPCV-UHFFFAOYSA-N 0.000 description 1
210000005260 human cell Anatomy 0.000 description 1
229910052588 hydroxylapatite Inorganic materials 0.000 description 1
125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 description 1
230000001900 immune effect Effects 0.000 description 1
238000011065 in-situ storage Methods 0.000 description 1
230000003993 interaction Effects 0.000 description 1
238000011835 investigation Methods 0.000 description 1
238000002955 isolation Methods 0.000 description 1
229950001103 ketoxal Drugs 0.000 description 1
239000011777 magnesium Substances 0.000 description 1
229910052749 magnesium Inorganic materials 0.000 description 1
229910052748 manganese Inorganic materials 0.000 description 1
239000011572 manganese Substances 0.000 description 1
210000003205 muscle Anatomy 0.000 description 1
VTPSNRIENVXKCI-UHFFFAOYSA-N n-(2,4-dimethylphenyl)-3-hydroxynaphthalene-2-carboxamide Chemical compound CC1=CC(C)=CC=C1NC(=O)C1=CC2=CC=CC=C2C=C1O VTPSNRIENVXKCI-UHFFFAOYSA-N 0.000 description 1
JPXMTWWFLBLUCD-UHFFFAOYSA-N nitro blue tetrazolium(2+) Chemical compound COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 JPXMTWWFLBLUCD-UHFFFAOYSA-N 0.000 description 1
238000003199 nucleic acid amplification method Methods 0.000 description 1
238000006384 oligomerization reaction Methods 0.000 description 1
238000007254 oxidation reaction Methods 0.000 description 1
238000004806 packaging method and process Methods 0.000 description 1
230000003071 parasitic effect Effects 0.000 description 1
XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 1
235000021317 phosphate Nutrition 0.000 description 1
125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
229920003023 plastic Polymers 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
238000012545 processing Methods 0.000 description 1
238000000746 purification Methods 0.000 description 1
210000004915 pus Anatomy 0.000 description 1
230000005855 radiation Effects 0.000 description 1
238000000163 radioactive labelling Methods 0.000 description 1
238000003127 radioimmunoassay Methods 0.000 description 1
238000012552 review Methods 0.000 description 1
210000003296 saliva Anatomy 0.000 description 1
238000013341 scale-up Methods 0.000 description 1
210000000582 semen Anatomy 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
239000011734 sodium Substances 0.000 description 1
239000007974 sodium acetate buffer Substances 0.000 description 1
239000001488 sodium phosphate Substances 0.000 description 1
229910000162 sodium phosphate Inorganic materials 0.000 description 1
235000010339 sodium tetraborate Nutrition 0.000 description 1
239000007790 solid phase Substances 0.000 description 1
239000002904 solvent Substances 0.000 description 1
238000003860 storage Methods 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
238000012546 transfer Methods 0.000 description 1
238000013519 translation Methods 0.000 description 1
BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
241001529453 unidentified herpesvirus Species 0.000 description 1
229940035893 uracil Drugs 0.000 description 1
210000002700 urine Anatomy 0.000 description 1
230000003612 virological effect Effects 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Wood Science & Technology (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Genetics & Genomics (AREA)
Molecular Biology (AREA)
Zoology (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Physics & Mathematics (AREA)
General Health & Medical Sciences (AREA)
Immunology (AREA)
Microbiology (AREA)
Hematology (AREA)
Urology & Nephrology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Cell Biology (AREA)
Plant Pathology (AREA)
Food Science & Technology (AREA)
Medicinal Chemistry (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Investigating Or Analysing Biological Materials (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

NO842720A 1983-07-05 1984-07-04 In vivo markering av polynukleotidsekvenser NO842720L (no)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US51097583A	1983-07-05	1983-07-05

Publications (1)

Publication Number	Publication Date
NO842720L true NO842720L (no)	1985-01-07

Family

ID=24032961

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO842720A NO842720L (no)	1983-07-05	1984-07-04	In vivo markering av polynukleotidsekvenser

Country Status (10)

Country	Link
EP (1)	EP0133473B1 (da)
JP (2)	JP2540482B2 (da)
AT (1)	ATE103327T1 (da)
AU (1)	AU581620B2 (da)
CA (1)	CA1338856C (da)
DE (1)	DE3486290T2 (da)
DK (1)	DK328084A (da)
ES (2)	ES8606229A1 (da)
IL (1)	IL72302A0 (da)
NO (1)	NO842720L (da)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IL75142A0 (en) *	1984-05-15	1985-09-29	Smithkline Beckman Corp	Polynucleotide hybridization probes
CA1277931C (en) *	1984-06-05	1990-12-18	Shimon Ulitzur	Detection and/or identification of microorganisms in a test sample usingbioluminescence or other exogenous genetically-introduced marker
NO870613L (no) *	1986-03-05	1987-09-07	Molecular Diagnostics Inc	Deteksjon av mikroorganismer i en prve inneholdende nukleinsyre.
FR2596773B1 (fr) *	1986-04-04	1988-06-24	Pasteur Institut	Sonde monocatenaire marquee, non radioactive, procede pour sa fabrication et procede de detection d'une sequence nucleotidique determinee a l'aide de cette sonde
SE455600B (sv) *	1986-11-27	1988-07-25	Svalof Ab	Forfarande for detektering av vextvirus och dess tillempning for detektering av rodsotvirus och potatisvirus
AU601021B2 (en) *	1987-03-11	1990-08-30	Molecular Diagnostics, Inc.	Assay for necleic acid sequences in a sample
GB2209754A (en) *	1987-09-17	1989-05-24	Thomas Lee Mattson	Polyaldehydic polynucleotides in use as probes,their preparation and use
US6326136B1 (en)	1988-04-01	2001-12-04	Digene Corporation	Macromolecular conjugate made using unsaturated aldehydes
AU2945792A (en) *	1991-11-25	1993-06-28	Keygene N.V.	A novel pcr method with a single primer for nucleic acid analysis
WO1997008345A1 (en) *	1995-08-30	1997-03-06	New York Medical College	Methods for labeling dna ends with halogenated nucleotides and detecting same with antibodies
DE19850594A1 (de) *	1998-11-03	2000-05-04	Biochip Technologies Gmbh	Verfahren zur Markierung von Nukleinsäuren
DE102006038686A1 (de) *	2006-08-17	2008-02-21	Forschungszentrum Karlsruhe Gmbh	Verfahren zur nicht radioaktiven Markierung von Ribonukleinsäure

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CA1223831A (en) *	1982-06-23	1987-07-07	Dean Engelhardt	Modified nucleotides, methods of preparing and utilizing and compositions containing the same
US4556643A (en) *	1982-07-26	1985-12-03	Agracetus	Assay method and probe for polynucleotide sequences
IL70765A (en) *	1983-01-27	1988-07-31	Enzo Biochem Inc	Substrates containing non-radioactive chemically-labeled polynucleotides and methods using them
CA1228811A (en) *	1983-05-05	1987-11-03	Robert G. Pergolizzi	Assay method utilizing polynucleotide sequences

1984
- 1984-06-28 CA CA000457804A patent/CA1338856C/en not_active Expired - Lifetime
- 1984-07-03 AU AU30205/84A patent/AU581620B2/en not_active Ceased
- 1984-07-04 DE DE3486290T patent/DE3486290T2/de not_active Expired - Lifetime
- 1984-07-04 ES ES534014A patent/ES8606229A1/es not_active Expired
- 1984-07-04 EP EP84107772A patent/EP0133473B1/en not_active Expired - Lifetime
- 1984-07-04 DK DK328084A patent/DK328084A/da not_active Application Discontinuation
- 1984-07-04 NO NO842720A patent/NO842720L/no unknown
- 1984-07-04 AT AT84107772T patent/ATE103327T1/de not_active IP Right Cessation
- 1984-07-04 JP JP59137381A patent/JP2540482B2/ja not_active Expired - Lifetime
- 1984-07-05 IL IL72302A patent/IL72302A0/xx unknown
1985
- 1985-04-16 ES ES542287A patent/ES8606651A1/es not_active Expired
1995
- 1995-12-20 JP JP7332416A patent/JP2607058B2/ja not_active Expired - Fee Related

Also Published As

Publication number	Publication date
JP2540482B2 (ja)	1996-10-02
ES8606229A1 (es)	1986-04-01
DK328084A (da)	1985-01-06
EP0133473A3 (en)	1987-02-04
EP0133473A2 (en)	1985-02-27
EP0133473B1 (en)	1994-03-23
ES8606651A1 (es)	1986-04-01
DE3486290T2 (de)	1994-07-14
ES542287A0 (es)	1986-04-01
ATE103327T1 (de)	1994-04-15
JPH08224084A (ja)	1996-09-03
CA1338856C (en)	1997-01-21
JP2607058B2 (ja)	1997-05-07
IL72302A0 (en)	1984-11-30
JPS6076667A (ja)	1985-05-01
AU3020584A (en)	1985-01-10
DE3486290D1 (de)	1994-04-28
ES534014A0 (es)	1986-04-01
DK328084D0 (da)	1984-07-04
AU581620B2 (en)	1989-03-02

Publication	Publication Date	Title
US4987065A (en)	1991-01-22	In vivo labelling of polynucleotide sequences
US5928862A (en)	1999-07-27	Competitive homogeneous assay
JP2577881B2 (ja)	1997-02-05	ポリヌクレオチド連鎖を利用する検定方法
CN102373273B (zh)	2013-07-03	一种结核分枝杆菌核酸的检测试剂盒
RU2107730C1 (ru)	1998-03-27	Молекулярный зонд
US5082935A (en)	1992-01-21	Diagnostic reagents made by attaching cytidine containing nucleic acid probes to amino functionalized solid supports by bisulfite mediated transamination
US5580971A (en)	1996-12-03	Fungal detection system based on rRNA probes
DE69734576T2 (de)	2006-08-03	Methode zur vervielfältigung und zum nachweis eines gewünschten nukleinsäurefragments
DK164407B (da)	1992-06-22	Modificerede nucleotider og fremgangsmaader til deres fremstilling, fremgangsmaade til paavisning af et dobbeltstrenget polynucleotidduplex indeholdende et modificeret nucleotid, fremgangsmaade til bestemmelse af tilstedevaerelsen af et deoxyribonucleinsyremolekyle eller et ribonucleinsyremolekyle, fremgangsmaade til test af en bakterie, fremgangsmaade til diagnosticering af en genetisk forstyrrelse, fre
KR19990067080A (ko)	1999-08-16	특정 뉴클레오티드 서열의 검출방법 및 조성물
Viscidi et al.	1987	Molecular diagnosis of infectious diseases by nucleic acid hybridization
NO842720L (no)	1985-01-07	In vivo markering av polynukleotidsekvenser
US5545521A (en)	1996-08-13	Method for detecting bioindividuals by use of nonnatural type nucleic acid probe
AU773714B2 (en)	2004-06-03	Cell assay, method and reagents
CA2075858C (en)	1998-05-19	Detection of complementary nucleotide sequences
EP2557156A1 (en)	2013-02-13	Primer and probe for detecting chlamydia trachomatis, and method for detecting chlamydia trachomatis using same
WO1997032044A9 (en)	1997-11-20	A method for the amplification and detection of a nucleic acid fragment of interest
PT687737E (pt)	2001-01-31	Deteccao de treponema pallidum e haemophilus ducreyi
US5314801A (en)	1994-05-24	Probes to Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium paratuberculosis
JPH08501220A (ja)	1996-02-13	Ｎｅｉｓｓｅｒｉａｇｏｎｏｒｒｈｏｅａｅ同定用の単離されたヌクレオチド配列
NO882593L (no)	1988-06-13	Forbedret nykleinsyrehybridiseringsteknikk og kit til dette.
JPS63282659A (ja)	1988-11-18	核酸の測定方法および測定剤
DE60208787T2 (de)	2006-11-02	Verfahren zum messen der polymerisierung von dna und anwendungen des verfahrens
DE60029939T2 (de)	2007-05-10	Diagnoseverfahren für whipples krankheit
JP2000342261A (ja)	2000-12-12	ブルクホルデリア・セパシアの検出法及び検出用キット