NO922794L - Fremgangsmaate for paavisning av nukleinsyrer, anvendelse av en nukleinsyre til immobilisering av nukleinsyrer, samt system for paavisning av nukleinsyrer - Google Patents
Fremgangsmaate for paavisning av nukleinsyrer, anvendelse av en nukleinsyre til immobilisering av nukleinsyrer, samt system for paavisning av nukleinsyrerInfo
- Publication number
- NO922794L NO922794L NO92922794A NO922794A NO922794L NO 922794 L NO922794 L NO 922794L NO 92922794 A NO92922794 A NO 92922794A NO 922794 A NO922794 A NO 922794A NO 922794 L NO922794 L NO 922794L
- Authority
- NO
- Norway
- Prior art keywords
- nucleic acids
- nucleic acid
- detection
- immobilization
- immobilizable
- Prior art date
Links
- 108020004707 nucleic acids Proteins 0.000 title abstract 11
- 150000007523 nucleic acids Chemical class 0.000 title abstract 11
- 102000039446 nucleic acids Human genes 0.000 title abstract 11
- 238000001514 detection method Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 6
- 125000003729 nucleotide group Chemical class 0.000 abstract 6
- 108020004711 Nucleic Acid Probes Proteins 0.000 abstract 2
- 239000002853 nucleic acid probe Substances 0.000 abstract 2
- 239000012491 analyte Substances 0.000 abstract 1
- 230000003100 immobilizing effect Effects 0.000 abstract 1
- 239000007790 solid phase Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Fremgangsmåte for påvisning av en analyttnukleinsyre som inneholder trinnene med hybridisering av en påvis- bart merket nukleinsyre eller en påvis- bart merket nukleinsyrehybrid med en immobiliserbar nukleinsyresonde, immobilisering av den dannede hybrid og påvisning av mengden av immobilisert hybrid over mengden av merking, hvor den immobiliserbare nukleinsyresonde inneholder to eller flere nukleotider som er modifisert med immobiliserbare grupper som ikke er direkte nabostilt i nukleotidsekvensen. Anvendelse av en nukleinsyre som inneholder to eller flere nukleotider som er modifisert med immobiliserbare grupper, og som ikke er direkte nabo- stilt i nukleotidsekvensen, for immobi- lisering av nukleinsyrer. System for påvisning av nuklein- syrer som inneholder en eller flere nukleinsyrer som inneholder to eller flere nukleotider som er modifisert med immobiliserbare grupper, og som ikke er direkte nabostilt i nukleotidsekvensen, og minst en fastfase som har en spesi- fikk affinitet for de immobiliserbare grupper.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE4123540A DE4123540A1 (de) | 1991-07-16 | 1991-07-16 | Immobilisierung von nukleinsaeuren |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| NO922794D0 NO922794D0 (no) | 1992-07-15 |
| NO922794L true NO922794L (no) | 1993-01-18 |
Family
ID=6436267
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO92922794A NO922794L (no) | 1991-07-16 | 1992-07-15 | Fremgangsmaate for paavisning av nukleinsyrer, anvendelse av en nukleinsyre til immobilisering av nukleinsyrer, samt system for paavisning av nukleinsyrer |
Country Status (15)
| Country | Link |
|---|---|
| EP (1) | EP0523557B1 (no) |
| JP (1) | JP2644419B2 (no) |
| KR (1) | KR960004039B1 (no) |
| AT (1) | ATE132538T1 (no) |
| AU (1) | AU640089B2 (no) |
| CA (1) | CA2073989A1 (no) |
| DE (2) | DE4123540A1 (no) |
| DK (1) | DK0523557T3 (no) |
| ES (1) | ES2083629T3 (no) |
| FI (1) | FI923239L (no) |
| IE (1) | IE922298A1 (no) |
| IL (1) | IL102487A0 (no) |
| NO (1) | NO922794L (no) |
| NZ (1) | NZ243525A (no) |
| ZA (1) | ZA925259B (no) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4239531A1 (de) * | 1992-11-25 | 1994-05-26 | Gabor Dr Igloi | Reagenz zur Kupplung verschiedener Substanzen an Nukleinsäuren sowie Verfahren zu dessen Herstellung |
| CA2119701A1 (en) * | 1993-04-16 | 1994-10-17 | Colleen M. Nycz | Nucleic acid assay procedure |
| US6821727B1 (en) | 1993-11-15 | 2004-11-23 | Applera Corporation | Hybridization assay using self-quenching fluorescence probe |
| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| DE19516196A1 (de) * | 1995-05-08 | 1996-11-14 | Boehringer Mannheim Gmbh | Verfahren zum quantitativen Nachweis von Nukleinsäuren |
| DE19525632C2 (de) * | 1995-07-14 | 1997-07-17 | Bag Biolog Analysensystem Gmbh | Sequenzspezifisches Nukleinsäurenachweisverfahren und Reagenz-System zu dessen Durchführung |
| DE19533354C2 (de) * | 1995-09-08 | 1999-07-01 | Derya Dr Ozkan | Verfahren und ihre Anwendungen um Nucleinsäuren sequenzspezifisch nachzuweisen und zu quantifizieren |
| DE19537952A1 (de) | 1995-10-12 | 1997-04-17 | Boehringer Mannheim Gmbh | Verfahren zum Nachweis eines Analyten |
| DE19548680A1 (de) * | 1995-12-23 | 1997-06-26 | Boehringer Mannheim Gmbh | Verfahren zum quantitativen Nachweis von spezifischen Nukleinsäuresequenzen |
| US5840879A (en) * | 1996-12-06 | 1998-11-24 | Wang; Edge R. | Reagents and solid supports for improved synthesis and labeling of polynucleotides |
| ES2129001B1 (es) * | 1997-07-18 | 2000-04-16 | Pharmagen S A | Metodo para la deteccion y visualizacion de una secuencia especifica de dna. |
| DE19835856A1 (de) * | 1998-08-07 | 2000-02-17 | W Kurt Roth | Verfahren zum Nachweis von HBV |
| DE102022204638B4 (de) * | 2022-05-12 | 2025-01-02 | Robert Bosch Gesellschaft mit beschränkter Haftung | Verfahren und mikrofluidische Vorrichtung zur Denaturierung einer Nukleinsäure |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4582789A (en) * | 1984-03-21 | 1986-04-15 | Cetus Corporation | Process for labeling nucleic acids using psoralen derivatives |
| US4868105A (en) * | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
| JPS638396A (ja) * | 1986-06-30 | 1988-01-14 | Wakunaga Pharmaceut Co Ltd | ポリ標識化オリゴヌクレオチド誘導体 |
| JPS6455198A (en) * | 1987-06-11 | 1989-03-02 | Nippon D P C Corp | Method for hybridization of nucleic acid |
| US5082830A (en) * | 1988-02-26 | 1992-01-21 | Enzo Biochem, Inc. | End labeled nucleotide probe |
| US5185243A (en) * | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
-
1991
- 1991-07-16 DE DE4123540A patent/DE4123540A1/de not_active Withdrawn
-
1992
- 1992-01-01 ZA ZA925259A patent/ZA925259B/xx unknown
- 1992-07-09 AU AU19534/92A patent/AU640089B2/en not_active Ceased
- 1992-07-10 EP EP92111743A patent/EP0523557B1/de not_active Expired - Lifetime
- 1992-07-10 DE DE59204886T patent/DE59204886D1/de not_active Expired - Lifetime
- 1992-07-10 AT AT92111743T patent/ATE132538T1/de not_active IP Right Cessation
- 1992-07-10 DK DK92111743.8T patent/DK0523557T3/da active
- 1992-07-10 ES ES92111743T patent/ES2083629T3/es not_active Expired - Lifetime
- 1992-07-13 IL IL102487A patent/IL102487A0/xx unknown
- 1992-07-13 NZ NZ243525A patent/NZ243525A/en unknown
- 1992-07-15 IE IE229892A patent/IE922298A1/en not_active Application Discontinuation
- 1992-07-15 NO NO92922794A patent/NO922794L/no unknown
- 1992-07-15 FI FI923239A patent/FI923239L/fi not_active Application Discontinuation
- 1992-07-16 KR KR1019920012681A patent/KR960004039B1/ko not_active Expired - Lifetime
- 1992-07-16 CA CA002073989A patent/CA2073989A1/en not_active Abandoned
- 1992-07-16 JP JP4189189A patent/JP2644419B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| NO922794D0 (no) | 1992-07-15 |
| AU640089B2 (en) | 1993-08-12 |
| ATE132538T1 (de) | 1996-01-15 |
| KR930002371A (ko) | 1993-02-23 |
| EP0523557B1 (de) | 1996-01-03 |
| EP0523557A1 (de) | 1993-01-20 |
| FI923239A7 (fi) | 1993-01-17 |
| CA2073989A1 (en) | 1993-01-17 |
| IE922298A1 (en) | 1993-01-27 |
| NZ243525A (en) | 1993-11-25 |
| DK0523557T3 (da) | 1996-05-06 |
| IL102487A0 (en) | 1993-01-14 |
| AU1953492A (en) | 1993-04-29 |
| JPH05199899A (ja) | 1993-08-10 |
| JP2644419B2 (ja) | 1997-08-25 |
| DE4123540A1 (de) | 1993-01-21 |
| ZA925259B (en) | 1994-01-17 |
| FI923239L (fi) | 1993-01-17 |
| DE59204886D1 (de) | 1996-02-15 |
| KR960004039B1 (ko) | 1996-03-25 |
| FI923239A0 (fi) | 1992-07-15 |
| ES2083629T3 (es) | 1996-04-16 |
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