ES2083629T3 - Inmovilizacion de acidos nucleicos. - Google Patents

Inmovilizacion de acidos nucleicos.

Info

Publication number
ES2083629T3
ES2083629T3 ES92111743T ES92111743T ES2083629T3 ES 2083629 T3 ES2083629 T3 ES 2083629T3 ES 92111743 T ES92111743 T ES 92111743T ES 92111743 T ES92111743 T ES 92111743T ES 2083629 T3 ES2083629 T3 ES 2083629T3
Authority
ES
Spain
Prior art keywords
nucleic acids
immobilization
testing
nucleotide sequence
acid probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92111743T
Other languages
English (en)
Inventor
Sibylle Dipl Chem Dr Berner
Stephanie Dipl Biol Dr Kohler
Cornelia Dipl Che Kruse-Muller
Rudolf Dr Seibl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2083629T3 publication Critical patent/ES2083629T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

PROCESO PARA LA COMPROBACION DE ACIDOS NUCLEICOS, EN LOS QUE UNA SONDA DE ACIDO NUCLEICO SE UTILIZA CON DOS O MAS NUCLEOTIDOS MODIFICADOS A TRAVES DE GRUPOS INMOVILIZABLES QUE NO SON VECINOS DE FORMA DIRECTA EN LA SECUENCIA DE NUCLEOTIDOS.
ES92111743T 1991-07-16 1992-07-10 Inmovilizacion de acidos nucleicos. Expired - Lifetime ES2083629T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE4123540A DE4123540A1 (de) 1991-07-16 1991-07-16 Immobilisierung von nukleinsaeuren

Publications (1)

Publication Number Publication Date
ES2083629T3 true ES2083629T3 (es) 1996-04-16

Family

ID=6436267

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92111743T Expired - Lifetime ES2083629T3 (es) 1991-07-16 1992-07-10 Inmovilizacion de acidos nucleicos.

Country Status (15)

Country Link
EP (1) EP0523557B1 (es)
JP (1) JP2644419B2 (es)
KR (1) KR960004039B1 (es)
AT (1) ATE132538T1 (es)
AU (1) AU640089B2 (es)
CA (1) CA2073989A1 (es)
DE (2) DE4123540A1 (es)
DK (1) DK0523557T3 (es)
ES (1) ES2083629T3 (es)
FI (1) FI923239A7 (es)
IE (1) IE922298A1 (es)
IL (1) IL102487A0 (es)
NO (1) NO922794L (es)
NZ (1) NZ243525A (es)
ZA (1) ZA925259B (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2129001A1 (es) * 1997-07-18 1999-05-16 Pharmagen S A Metodo para la deteccion y visualizacion de una secuencia especifica de dna.

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4239531A1 (de) * 1992-11-25 1994-05-26 Gabor Dr Igloi Reagenz zur Kupplung verschiedener Substanzen an Nukleinsäuren sowie Verfahren zu dessen Herstellung
CA2119701A1 (en) * 1993-04-16 1994-10-17 Colleen M. Nycz Nucleic acid assay procedure
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US6821727B1 (en) 1993-11-15 2004-11-23 Applera Corporation Hybridization assay using self-quenching fluorescence probe
DE19516196A1 (de) * 1995-05-08 1996-11-14 Boehringer Mannheim Gmbh Verfahren zum quantitativen Nachweis von Nukleinsäuren
DE19525632C2 (de) * 1995-07-14 1997-07-17 Bag Biolog Analysensystem Gmbh Sequenzspezifisches Nukleinsäurenachweisverfahren und Reagenz-System zu dessen Durchführung
DE19533354C2 (de) * 1995-09-08 1999-07-01 Derya Dr Ozkan Verfahren und ihre Anwendungen um Nucleinsäuren sequenzspezifisch nachzuweisen und zu quantifizieren
DE19537952A1 (de) 1995-10-12 1997-04-17 Boehringer Mannheim Gmbh Verfahren zum Nachweis eines Analyten
DE19548680A1 (de) * 1995-12-23 1997-06-26 Boehringer Mannheim Gmbh Verfahren zum quantitativen Nachweis von spezifischen Nukleinsäuresequenzen
US5840879A (en) * 1996-12-06 1998-11-24 Wang; Edge R. Reagents and solid supports for improved synthesis and labeling of polynucleotides
DE19835856A1 (de) * 1998-08-07 2000-02-17 W Kurt Roth Verfahren zum Nachweis von HBV
DE102022204638B4 (de) * 2022-05-12 2025-01-02 Robert Bosch Gesellschaft mit beschränkter Haftung Verfahren und mikrofluidische Vorrichtung zur Denaturierung einer Nukleinsäure

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4582789A (en) * 1984-03-21 1986-04-15 Cetus Corporation Process for labeling nucleic acids using psoralen derivatives
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
JPS638396A (ja) * 1986-06-30 1988-01-14 Wakunaga Pharmaceut Co Ltd ポリ標識化オリゴヌクレオチド誘導体
JPS6455198A (en) * 1987-06-11 1989-03-02 Nippon D P C Corp Method for hybridization of nucleic acid
US5082830A (en) * 1988-02-26 1992-01-21 Enzo Biochem, Inc. End labeled nucleotide probe
US5185243A (en) * 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2129001A1 (es) * 1997-07-18 1999-05-16 Pharmagen S A Metodo para la deteccion y visualizacion de una secuencia especifica de dna.

Also Published As

Publication number Publication date
JP2644419B2 (ja) 1997-08-25
CA2073989A1 (en) 1993-01-17
IE922298A1 (en) 1993-01-27
DK0523557T3 (da) 1996-05-06
NO922794L (no) 1993-01-18
EP0523557B1 (de) 1996-01-03
DE4123540A1 (de) 1993-01-21
EP0523557A1 (de) 1993-01-20
ZA925259B (en) 1994-01-17
KR930002371A (ko) 1993-02-23
IL102487A0 (en) 1993-01-14
AU1953492A (en) 1993-04-29
KR960004039B1 (ko) 1996-03-25
ATE132538T1 (de) 1996-01-15
JPH05199899A (ja) 1993-08-10
FI923239L (fi) 1993-01-17
AU640089B2 (en) 1993-08-12
FI923239A0 (fi) 1992-07-15
NZ243525A (en) 1993-11-25
NO922794D0 (no) 1992-07-15
FI923239A7 (fi) 1993-01-17
DE59204886D1 (de) 1996-02-15

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