OA10089A - In vitro cornea equivalent model - Google Patents

In vitro cornea equivalent model Download PDF

Info

Publication number: OA10089A
Authority: OA; OAPI
Prior art keywords: cells; layer; cornea; équivalent; collagen
Prior art date: 1992-11-13

Application number

OA60536A

Other languages

English (en)

Inventor

Nancy Louise Parenteau

Valerie Susan Mason

Bjorn Reino Olsen

Original Assignee

Organogenesis Inc

Harvard College

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1992-11-13

Filing date

1994-07-11

Publication date

1996-12-18

1994-07-11 Application filed by Organogenesis Inc, Harvard College filed Critical Organogenesis Inc

1996-12-18 Publication of OA10089A publication Critical patent/OA10089A/en

Links

210000004087 cornea Anatomy 0.000 title claims abstract description 33
238000000338 in vitro Methods 0.000 title claims abstract description 12
238000001727 in vivo Methods 0.000 claims abstract description 17
238000012258 culturing Methods 0.000 claims abstract description 14
238000000034 method Methods 0.000 claims abstract description 14
238000002054 transplantation Methods 0.000 claims abstract description 4
210000004027 cell Anatomy 0.000 claims description 112
229920001436 collagen Polymers 0.000 claims description 46
102000008186 Collagen Human genes 0.000 claims description 37
108010035532 Collagen Proteins 0.000 claims description 37
239000000203 mixture Substances 0.000 claims description 14
238000012360 testing method Methods 0.000 claims description 8
230000000694 effects Effects 0.000 claims description 6
101710128836 Large T antigen Proteins 0.000 claims description 4
108090000623 proteins and genes Proteins 0.000 claims description 3
210000002536 stromal cell Anatomy 0.000 claims description 3
239000000126 substance Substances 0.000 claims description 3
238000004519 manufacturing process Methods 0.000 claims 1
210000000056 organ Anatomy 0.000 abstract description 9
238000010874 in vitro model Methods 0.000 abstract description 3
150000001875 compounds Chemical class 0.000 abstract description 2
238000002513 implantation Methods 0.000 abstract description 2
238000012216 screening Methods 0.000 abstract 1
239000010410 layer Substances 0.000 description 74
210000002950 fibroblast Anatomy 0.000 description 20
210000000981 epithelium Anatomy 0.000 description 19
239000002609 medium Substances 0.000 description 16
210000001519 tissue Anatomy 0.000 description 16
239000012528 membrane Substances 0.000 description 15
238000004113 cell culture Methods 0.000 description 13
210000004379 membrane Anatomy 0.000 description 12
241000283973 Oryctolagus cuniculus Species 0.000 description 10
239000011159 matrix material Substances 0.000 description 10
102000012288 Phosphopyruvate Hydratase Human genes 0.000 description 9
108010022181 Phosphopyruvate Hydratase Proteins 0.000 description 9
210000002469 basement membrane Anatomy 0.000 description 9
NWIBSHFKIJFRCO-WUDYKRTCSA-N Mytomycin Chemical compound C1N2C(C(C(C)=C(N)C3=O)=O)=C3[C@@H](COC(N)=O)[C@@]2(OC)[C@@H]2[C@H]1N2 NWIBSHFKIJFRCO-WUDYKRTCSA-N 0.000 description 8
210000003560 epithelium corneal Anatomy 0.000 description 8
239000000499 gel Substances 0.000 description 8
229920000742 Cotton Polymers 0.000 description 7
239000006144 Dulbecco’s modiﬁed Eagle's medium Substances 0.000 description 7
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
230000005540 biological transmission Effects 0.000 description 6
230000015572 biosynthetic process Effects 0.000 description 6
238000009826 distribution Methods 0.000 description 6
238000011534 incubation Methods 0.000 description 6
238000010186 staining Methods 0.000 description 6
210000002435 tendon Anatomy 0.000 description 6
230000004888 barrier function Effects 0.000 description 5
230000035699 permeability Effects 0.000 description 5
229920000515 polycarbonate Polymers 0.000 description 5
239000004417 polycarbonate Substances 0.000 description 5
210000003491 skin Anatomy 0.000 description 5
241000283690 Bos taurus Species 0.000 description 4
102000005373 Keratin-3 Human genes 0.000 description 4
108010070918 Keratin-3 Proteins 0.000 description 4
208000027418 Wounds and injury Diseases 0.000 description 4
210000003683 corneal stroma Anatomy 0.000 description 4
238000011161 development Methods 0.000 description 4
239000012530 fluid Substances 0.000 description 4
230000007794 irritation Effects 0.000 description 4
238000012423 maintenance Methods 0.000 description 4
229960004857 mitomycin Drugs 0.000 description 4
210000001578 tight junction Anatomy 0.000 description 4
102000004510 Collagen Type VII Human genes 0.000 description 3
108010017377 Collagen Type VII Proteins 0.000 description 3
CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 3
229930182566 Gentamicin Natural products 0.000 description 3
239000012981 Hank's balanced salt solution Substances 0.000 description 3
208000012868 Overgrowth Diseases 0.000 description 3
102000003970 Vinculin Human genes 0.000 description 3
108090000384 Vinculin Proteins 0.000 description 3
230000002159 abnormal effect Effects 0.000 description 3
239000002253 acid Substances 0.000 description 3
244000309466 calf Species 0.000 description 3
230000035605 chemotaxis Effects 0.000 description 3
239000000306 component Substances 0.000 description 3
230000030944 contact inhibition Effects 0.000 description 3
238000010586 diagram Methods 0.000 description 3
239000000835 fiber Substances 0.000 description 3
GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 3
229960002518 gentamicin Drugs 0.000 description 3
230000003463 hyperproliferative effect Effects 0.000 description 3
239000000463 material Substances 0.000 description 3
238000004264 monolayer culture Methods 0.000 description 3
230000000877 morphologic effect Effects 0.000 description 3
210000005123 simple squamous epithelium Anatomy 0.000 description 3
239000000243 solution Substances 0.000 description 3
230000007480 spreading Effects 0.000 description 3
238000003892 spreading Methods 0.000 description 3
MEYZYGMYMLNUHJ-UHFFFAOYSA-N tunicamycin Natural products CC(C)CCCCCCCCCC=CC(=O)NC1C(O)C(O)C(CC(O)C2OC(C(O)C2O)N3C=CC(=O)NC3=O)OC1OC4OC(CO)C(O)C(O)C4NC(=O)C MEYZYGMYMLNUHJ-UHFFFAOYSA-N 0.000 description 3
HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 2
HGINCPLSRVDWNT-UHFFFAOYSA-N Acrolein Chemical compound C=CC=O HGINCPLSRVDWNT-UHFFFAOYSA-N 0.000 description 2
102000012422 Collagen Type I Human genes 0.000 description 2
108010022452 Collagen Type I Proteins 0.000 description 2
WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 2
241001465754 Metazoa Species 0.000 description 2
SUHOOTKUPISOBE-UHFFFAOYSA-N O-phosphoethanolamine Chemical compound NCCOP(O)(O)=O SUHOOTKUPISOBE-UHFFFAOYSA-N 0.000 description 2
241001494479 Pecora Species 0.000 description 2
UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
QIRDPEPUXNCOLD-UHFFFAOYSA-N [9-(diethylamino)benzo[a]phenoxazin-5-ylidene]azanium;sulfate Chemical compound [O-]S([O-])(=O)=O.C1=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=CC(=[NH2+])C2=C1.C1=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=CC(=[NH2+])C2=C1 QIRDPEPUXNCOLD-UHFFFAOYSA-N 0.000 description 2
238000004873 anchoring Methods 0.000 description 2
210000004045 bowman membrane Anatomy 0.000 description 2
238000005266 casting Methods 0.000 description 2
239000013553 cell monolayer Substances 0.000 description 2
230000001413 cellular effect Effects 0.000 description 2
210000002808 connective tissue Anatomy 0.000 description 2
238000010276 construction Methods 0.000 description 2
210000004207 dermis Anatomy 0.000 description 2
239000001963 growth medium Substances 0.000 description 2
229920000669 heparin Polymers 0.000 description 2
229960002897 heparin Drugs 0.000 description 2
JYGXADMDTFJGBT-VWUMJDOOSA-N hydrocortisone Chemical compound O=C1CC[C@]2(C)[C@H]3[C@@H](O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 JYGXADMDTFJGBT-VWUMJDOOSA-N 0.000 description 2
238000010166 immunofluorescence Methods 0.000 description 2
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
210000001232 limbus corneae Anatomy 0.000 description 2
150000002632 lipids Chemical class 0.000 description 2
239000003550 marker Substances 0.000 description 2
238000012986 modification Methods 0.000 description 2
230000004048 modification Effects 0.000 description 2
239000004033 plastic Substances 0.000 description 2
230000001737 promoting effect Effects 0.000 description 2
229920005989 resin Polymers 0.000 description 2
239000011347 resin Substances 0.000 description 2
230000004044 response Effects 0.000 description 2
230000008458 response to injury Effects 0.000 description 2
239000002356 single layer Substances 0.000 description 2
IHQKEDIOMGYHEB-UHFFFAOYSA-M sodium dimethylarsinate Chemical compound [Na+].C[As](C)([O-])=O IHQKEDIOMGYHEB-UHFFFAOYSA-M 0.000 description 2
231100000419 toxicity Toxicity 0.000 description 2
230000001988 toxicity Effects 0.000 description 2
230000009466 transformation Effects 0.000 description 2
KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
KYARBIJYVGJZLB-UHFFFAOYSA-N 7-amino-4-hydroxy-2-naphthalenesulfonic acid Chemical compound OC1=CC(S(O)(=O)=O)=CC2=CC(N)=CC=C21 KYARBIJYVGJZLB-UHFFFAOYSA-N 0.000 description 1
229930024421 Adenine Natural products 0.000 description 1
GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
229920000936 Agarose Polymers 0.000 description 1
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
102000004266 Collagen Type IV Human genes 0.000 description 1
108010042086 Collagen Type IV Proteins 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
102000002812 Heat-Shock Proteins Human genes 0.000 description 1
108010004889 Heat-Shock Proteins Proteins 0.000 description 1
102000004877 Insulin Human genes 0.000 description 1
108090001061 Insulin Proteins 0.000 description 1
102000011782 Keratins Human genes 0.000 description 1
108010076876 Keratins Proteins 0.000 description 1
FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
241000699666 Mus <mouse, genus> Species 0.000 description 1
239000004677 Nylon Substances 0.000 description 1
239000004264 Petrolatum Substances 0.000 description 1
239000004743 Polypropylene Substances 0.000 description 1
BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical compound IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 description 1
102000004338 Transferrin Human genes 0.000 description 1
108090000901 Transferrin Proteins 0.000 description 1
COQLPRJCUIATTQ-UHFFFAOYSA-N Uranyl acetate Chemical compound O.O.O=[U]=O.CC(O)=O.CC(O)=O COQLPRJCUIATTQ-UHFFFAOYSA-N 0.000 description 1
238000002835 absorbance Methods 0.000 description 1
229960000643 adenine Drugs 0.000 description 1
229940072056 alginate Drugs 0.000 description 1
229920000615 alginic acid Polymers 0.000 description 1
235000010443 alginic acid Nutrition 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
238000010171 animal model Methods 0.000 description 1
238000003556 assay Methods 0.000 description 1
230000002238 attenuated effect Effects 0.000 description 1
239000011324 bead Substances 0.000 description 1
230000008901 benefit Effects 0.000 description 1
239000007975 buffered saline Substances 0.000 description 1
229910052791 calcium Inorganic materials 0.000 description 1
239000011575 calcium Substances 0.000 description 1
239000001110 calcium chloride Substances 0.000 description 1
229910001628 calcium chloride Inorganic materials 0.000 description 1
239000006143 cell culture medium Substances 0.000 description 1
230000008619 cell matrix interaction Effects 0.000 description 1
229920002678 cellulose Polymers 0.000 description 1
239000001913 cellulose Substances 0.000 description 1
230000008859 change Effects 0.000 description 1
230000003399 chemotactic effect Effects 0.000 description 1
239000000512 collagen gel Substances 0.000 description 1
230000001143 conditioned effect Effects 0.000 description 1
210000000795 conjunctiva Anatomy 0.000 description 1
230000008602 contraction Effects 0.000 description 1
230000004453 corneal transparency Effects 0.000 description 1
238000012136 culture method Methods 0.000 description 1
210000004748 cultured cell Anatomy 0.000 description 1
230000003436 cytoskeletal effect Effects 0.000 description 1
230000003013 cytotoxicity Effects 0.000 description 1
231100000135 cytotoxicity Toxicity 0.000 description 1
210000001047 desmosome Anatomy 0.000 description 1
238000000502 dialysis Methods 0.000 description 1
LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
NJDNXYGOVLYJHP-UHFFFAOYSA-L disodium;2-(3-oxido-6-oxoxanthen-9-yl)benzoate Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=CC(=O)C=C2OC2=CC([O-])=CC=C21 NJDNXYGOVLYJHP-UHFFFAOYSA-L 0.000 description 1
YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 1
210000002615 epidermis Anatomy 0.000 description 1
239000003822 epoxy resin Substances 0.000 description 1
238000011156 evaluation Methods 0.000 description 1
210000002744 extracellular matrix Anatomy 0.000 description 1
210000000887 face Anatomy 0.000 description 1
210000003195 fascia Anatomy 0.000 description 1
239000011888 foil Substances 0.000 description 1
239000003365 glass fiber Substances 0.000 description 1
239000004519 grease Substances 0.000 description 1
210000000301 hemidesmosome Anatomy 0.000 description 1
229920002674 hyaluronan Polymers 0.000 description 1
229960003160 hyaluronic acid Drugs 0.000 description 1
229960000890 hydrocortisone Drugs 0.000 description 1
239000000017 hydrogel Substances 0.000 description 1
230000000984 immunochemical effect Effects 0.000 description 1
229940125396 insulin Drugs 0.000 description 1
230000008611 intercellular interaction Effects 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
238000002372 labelling Methods 0.000 description 1
FYDKNKUEBJQCCN-UHFFFAOYSA-N lanthanum(3+);trinitrate Chemical compound [La+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O FYDKNKUEBJQCCN-UHFFFAOYSA-N 0.000 description 1
230000004807 localization Effects 0.000 description 1
239000012533 medium component Substances 0.000 description 1
230000005499 meniscus Effects 0.000 description 1
238000000386 microscopy Methods 0.000 description 1
CBMFIOOVRYZOBT-DAWNIORJSA-N mitomycin k Chemical compound O=C1C(OC)=C(C)C(=O)C2=C1C(=C)[C@]1(OC)N2C[C@H]2[C@@H]1N2C CBMFIOOVRYZOBT-DAWNIORJSA-N 0.000 description 1
231100000286 mucous membrane, eye irritation or corrosion testing Toxicity 0.000 description 1
230000017066 negative regulation of growth Effects 0.000 description 1
235000015097 nutrients Nutrition 0.000 description 1
229920001778 nylon Polymers 0.000 description 1
230000008520 organization Effects 0.000 description 1
238000012261 overproduction Methods 0.000 description 1
229940066842 petrolatum Drugs 0.000 description 1
235000019271 petrolatum Nutrition 0.000 description 1
239000002953 phosphate buffered saline Substances 0.000 description 1
238000007747 plating Methods 0.000 description 1
229920000647 polyepoxide Polymers 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
-1 polypropylene Polymers 0.000 description 1
229920001155 polypropylene Polymers 0.000 description 1
229920001296 polysiloxane Polymers 0.000 description 1
230000003389 potentiating effect Effects 0.000 description 1
102000004196 processed proteins & peptides Human genes 0.000 description 1
108090000765 processed proteins & peptides Proteins 0.000 description 1
230000001681 protective effect Effects 0.000 description 1
239000002994 raw material Substances 0.000 description 1
230000001105 regulatory effect Effects 0.000 description 1
230000008439 repair process Effects 0.000 description 1
239000012679 serum free medium Substances 0.000 description 1
230000036556 skin irritation Effects 0.000 description 1
229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
235000017557 sodium bicarbonate Nutrition 0.000 description 1
241000894007 species Species 0.000 description 1
229910001631 strontium chloride Inorganic materials 0.000 description 1
AHBGXTDRMVNFER-UHFFFAOYSA-L strontium dichloride Chemical compound [Cl-].[Cl-].[Sr+2] AHBGXTDRMVNFER-UHFFFAOYSA-L 0.000 description 1
239000004094 surface-active agent Substances 0.000 description 1
239000000725 suspension Substances 0.000 description 1
230000000699 topical effect Effects 0.000 description 1
238000002723 toxicity assay Methods 0.000 description 1
231100000027 toxicology Toxicity 0.000 description 1
239000012581 transferrin Substances 0.000 description 1
229940035722 triiodothyronine Drugs 0.000 description 1
210000003606 umbilical vein Anatomy 0.000 description 1
241001430294 unidentified retrovirus Species 0.000 description 1
230000037314 wound repair Effects 0.000 description 1

Classifications

- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
- A61L27/3891—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types as distinct cell layers
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/14—Eye parts, e.g. lenses or corneal implants; Artificial eyes
- A61F2/142—Cornea, e.g. artificial corneae, keratoprostheses or corneal implants for repair of defective corneal tissue
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3808—Endothelial cells
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3813—Epithelial cells, e.g. keratinocytes, urothelial cells
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0062—General methods for three-dimensional culture
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/16—Materials or treatment for tissue regeneration for reconstruction of eye parts, e.g. intraocular lens, cornea
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/395—Thyroid hormones
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/08—Coculture with; Conditioned medium produced by cells of the nervous system
- C12N2502/085—Coculture with; Conditioned medium produced by cells of the nervous system eye cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
- C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1323—Adult fibroblasts
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/04—Immortalised cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Chemical & Material Sciences (AREA)
Zoology (AREA)
Cell Biology (AREA)
General Health & Medical Sciences (AREA)
Veterinary Medicine (AREA)
Transplantation (AREA)
Animal Behavior & Ethology (AREA)
Public Health (AREA)
Medicinal Chemistry (AREA)
Epidemiology (AREA)
Oral & Maxillofacial Surgery (AREA)
Dermatology (AREA)
Biotechnology (AREA)
Botany (AREA)
Chemical Kinetics & Catalysis (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
Wood Science & Technology (AREA)
Biochemistry (AREA)
Microbiology (AREA)
Urology & Nephrology (AREA)
General Engineering & Computer Science (AREA)
Ophthalmology & Optometry (AREA)
Vascular Medicine (AREA)
Developmental Biology & Embryology (AREA)
Heart & Thoracic Surgery (AREA)
Virology (AREA)
Cardiology (AREA)
Immunology (AREA)
Biophysics (AREA)
Neurology (AREA)
Neurosurgery (AREA)
Pharmacology & Pharmacy (AREA)
Materials For Medical Uses (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

OA60536A 1992-11-13 1994-07-11 In vitro cornea equivalent model OA10089A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US07/974,740 US5374515A (en)	1992-11-13	1992-11-13	In vitro cornea equivalent model

Publications (1)

Publication Number	Publication Date
OA10089A true OA10089A (en)	1996-12-18

Family

ID=25522389

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
OA60536A OA10089A (en)	1992-11-13	1994-07-11	In vitro cornea equivalent model

Country Status (13)

Country	Link
US (1)	US5374515A (ja)
EP (1)	EP0621766B1 (ja)
JP (1)	JPH08500041A (ja)
CN (1)	CN1080130C (ja)
AT (1)	ATE202688T1 (ja)
CA (1)	CA2127120A1 (ja)
DE (1)	DE69330402T2 (ja)
DK (1)	DK0621766T3 (ja)
ES (1)	ES2160116T3 (ja)
GR (1)	GR3036778T3 (ja)
OA (1)	OA10089A (ja)
PT (1)	PT621766E (ja)
WO (1)	WO1994010940A1 (ja)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6514238B1 (en) *	1989-08-14	2003-02-04	Photogenesis, Inc.	Method for preparation and transplantation of volute grafts and surgical instrument therefor
SG49267A1 (en) *	1989-08-14	1998-05-18	Photogenesis Inc	Surgical instrument and cell isolation and transplantation
US5817075A (en) *	1989-08-14	1998-10-06	Photogenesis, Inc.	Method for preparation and transplantation of planar implants and surgical instrument therefor
US6045791A (en) *	1992-03-06	2000-04-04	Photogenesis, Inc.	Retinal pigment epithelium transplantation
IL106922A (en) *	1992-09-14	1998-08-16	Novartis Ag	Composite materials with one or more wettable surfaces and process for their preparation
US5827641A (en) *	1992-11-13	1998-10-27	Parenteau; Nancy L.	In vitro cornea equivalent model
US5672498A (en) *	1993-12-28	1997-09-30	The Gillete Company	Human corneal epithelial cell lines with extended lifespan
US6334872B1 (en)	1994-02-18	2002-01-01	Organogenesis Inc.	Method for treating diseased or damaged organs
US5763255A (en) *	1994-04-25	1998-06-09	Becton Dickinson And Company	Inducing epithelial cell differentiation with dried native fibrillar collagen
US5858781A (en) *	1994-05-13	1999-01-12	Matyas; John R.	Method of tissue transfer and retrieval
US5695996A (en) *	1994-09-23	1997-12-09	The United States Of America As Represented By The Department Of Health And Human Services	Artificial organ culture system
US5868728A (en) *	1995-02-28	1999-02-09	Photogenesis, Inc.	Medical linear actuator for surgical delivery, manipulation, and extraction
US20020095218A1 (en) *	1996-03-12	2002-07-18	Carr Robert M.	Tissue repair fabric
US8716227B2 (en)	1996-08-23	2014-05-06	Cook Biotech Incorporated	Graft prosthesis, materials and methods
CZ54899A3 (cs)	1996-08-23	1999-08-11	Cook Biotech, Incorporated	Štěpová protéza, materiály s ní spojené a způsoby její výroby
US6666892B2 (en)	1996-08-23	2003-12-23	Cook Biotech Incorporated	Multi-formed collagenous biomaterial medical device
AU740113B2 (en) *	1997-02-20	2001-11-01	Gerigene Medical Corporation	Augmentation and repair of dermal, subcutaneous, and vocal cord tissue defects
US20020086423A1 (en) *	1997-09-09	2002-07-04	Toshiaki Takezawa	Hydrogel thin film containing extracellular matrix components
CA2227827A1 (en) *	1998-01-23	1999-07-23	Unknown	In vitro artificial cornea and sclera
CA2334228C (en)	1998-06-05	2010-09-28	Organogenesis Inc.	Bioengineered vascular graft support prostheses
AU753773B2 (en)	1998-06-05	2002-10-31	Organogenesis Inc.	Bioengineered vascular graft prostheses
EP1082057B1 (en)	1998-06-05	2009-02-25	Organogenesis Inc.	Bioengineered tubular graft prostheses
CA2334364C (en)	1998-06-05	2011-01-04	Organogenesis Inc.	Bioengineered flat sheet graft prostheses
JP2002530069A (ja) *	1998-11-19	2002-09-17	オルガノジェネシスインク．	生物工学的組織構築物およびそれを生成および使用する方法
US8882850B2 (en)	1998-12-01	2014-11-11	Cook Biotech Incorporated	Multi-formed collagenous biomaterial medical device
EP1051116B8 (en)	1998-12-01	2009-06-10	Washington University	Embolization device
US6030789A (en) *	1999-03-10	2000-02-29	The Gillette Company	Human conjunctival epithelial cell lines with an extended life span
US7073510B2 (en) *	2000-02-11	2006-07-11	The General Hospital Corporation	Photochemical tissue bonding
US8215314B2 (en) *	2000-02-11	2012-07-10	The General Hospital Corporation	Photochemical tissue bonding
EP1272119B1 (en)	2000-02-11	2010-11-24	The General Hospital Corporation	Photochemical tissue bonding
US20020039788A1 (en) *	2000-02-29	2002-04-04	Isseroff Roslyn R.	Corneal epithelial graft composites
US6544286B1 (en) *	2000-07-18	2003-04-08	Tissue Engineering Refraction, Inc.	Pre-fabricated corneal tissue lens method of corneal overlay to correct vision
JP4554051B2 (ja) *	2000-09-08	2010-09-29	Ｈｏｙａ株式会社	角膜の再構築方法
EP1320390A2 (en) *	2000-09-18	2003-06-25	Organogenesis Inc.	Bioengineered flat sheet graft prosthesis and its use
US7156859B2 (en)	2001-07-23	2007-01-02	Fos Holding S.A.	Device for separating the epithelium layer from the surface of the cornea of an eye
KR20040024859A (ko) *	2001-07-25	2004-03-22	가부시키가이샤 재팬 티슈 엔지니어링	각막 치료용 적층체 및 그 제조방법
EP1474084A4 (en) *	2002-01-17	2007-04-18	Edward Perez	METHODS FOR REALIZING EPITHELIAL RECOVERY ON THE CORNEA AND FOR PLACING OCULAR DEVICES AND LENSES UNDER EPITHELIAL RECOVERY OR EPITHELIAL MEMBRANE, EPITHELIAL DETACHING DEVICES, EPITHELIUM STRUCTURES, DEVICES AND OCULAR LENSES
US7189245B2 (en) *	2002-02-14	2007-03-13	Photogenesis, Inc.	Subretinal implantation device and surgical cannulas for use therewith
CA2483659A1 (en) *	2002-04-30	2003-11-13	Amniotec Inc.	Corneal endothelium-like sheet and method of constructing the same
US7919270B2 (en) *	2003-09-09	2011-04-05	Acyte Biotech Pty Ltd	Mammalian expression system
JP4834802B2 (ja) *	2004-02-18	2011-12-14	聡山上	ヒト角膜内皮細胞の培養物層積層体及びその作製方法
US7909867B2 (en) *	2004-10-05	2011-03-22	The Board Of Trustees Of The Leland Stanford Junior University	Interpenetrating polymer network hydrogel corneal prosthesis
US7857447B2 (en) *	2004-10-05	2010-12-28	The Board Of Trustees Of The Leland Stanford Junior University	Interpenetrating polymer network hydrogel contact lenses
US7857849B2 (en) *	2004-10-05	2010-12-28	The Board Of Trustees Of The Leland Stanford Junior Iniversity	Artificial corneal implant
US20090088846A1 (en)	2007-04-17	2009-04-02	David Myung	Hydrogel arthroplasty device
JP5255846B2 (ja) *	2006-01-19	2013-08-07	千寿製薬株式会社	生体内で細胞増殖可能な角膜内皮製剤
FR2927632B1 (fr) *	2008-02-14	2013-07-19	Basf Beauty Care Solutions F	Cornee et muqueuse reconstruites.
US8883915B2 (en)	2008-07-07	2014-11-11	Biomimedica, Inc.	Hydrophobic and hydrophilic interpenetrating polymer networks derived from hydrophobic polymers and methods of preparing the same
US20120209396A1 (en)	2008-07-07	2012-08-16	David Myung	Orthopedic implants having gradient polymer alloys
US8497023B2 (en)	2008-08-05	2013-07-30	Biomimedica, Inc.	Polyurethane-grafted hydrogels
US20100120013A1 (en) *	2008-11-07	2010-05-13	Mb Research Laboratories, Inc.	Procedure for long term corneal culture
EP2755598B1 (en) *	2011-09-16	2019-03-06	Wake Forest University Health Sciences	Fabrication of gelatin hydrogel sheet for the transplantation of corneal endothelium
EP3357518B1 (en)	2011-10-03	2020-12-02	Hyalex Orthopaedics, Inc.	Polymeric adhesive for anchoring compliant materials to another surface
EP2782524B1 (en)	2011-11-21	2017-12-20	Biomimedica, Inc	Systems for anchoring orthopaedic implants to bone
WO2014015274A1 (en)	2012-07-20	2014-01-23	The General Hospital Corporation	Methods for tissue passivation
US10549112B2 (en)	2012-07-20	2020-02-04	The General Hospital Corporation	Apparatus for tissue irradiation and methods and kits utilizing the same
CA3278502A1 (en)	2013-07-18	2025-10-31	The General Hospital Corporation	Vessel treatment systems, methods, and kits
US9238090B1 (en)	2014-12-24	2016-01-19	Fettech, Llc	Tissue-based compositions
US11077228B2 (en)	2015-08-10	2021-08-03	Hyalex Orthopaedics, Inc.	Interpenetrating polymer networks
US11645951B2 (en) *	2018-06-19	2023-05-09	EBM Corporation	Artificial organ model for training for surgical technique, method for manufacturing artificial organ model, and method for training for surgical technique using artificial organ model
US10869950B2 (en)	2018-07-17	2020-12-22	Hyalex Orthopaedics, Inc.	Ionic polymer compositions
GB201916506D0 (en) *	2019-11-13	2019-12-25	Univ Nottingham	Corneal tissue
WO2022004938A1 (ko) *	2020-06-30	2022-01-06	주식회사 미래셀바이오	유사 중간엽 줄기세포의 제조방법

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4546500A (en) *	1981-05-08	1985-10-15	Massachusetts Institute Of Technology	Fabrication of living blood vessels and glandular tissues
US4485096A (en) *	1982-02-26	1984-11-27	Massachusetts Institute Of Technology	Tissue-equivalent and method for preparation thereof
US4760020A (en) *	1985-05-03	1988-07-26	Eye Research Institute Of Retina Foundation	Method for testing biocompatibility
ES2004281A6 (es) *	1986-04-04	1988-12-16	Univ Jefferson	Una superficie protesica implantable para implantacion en un paciente humano

1992
- 1992-11-13 US US07/974,740 patent/US5374515A/en not_active Expired - Fee Related
1993
- 1993-11-13 CN CN93121325A patent/CN1080130C/zh not_active Expired - Fee Related
- 1993-11-15 ES ES94901313T patent/ES2160116T3/es not_active Expired - Lifetime
- 1993-11-15 CA CA002127120A patent/CA2127120A1/en not_active Abandoned
- 1993-11-15 WO PCT/US1993/010727 patent/WO1994010940A1/en not_active Ceased
- 1993-11-15 PT PT94901313T patent/PT621766E/pt unknown
- 1993-11-15 DK DK94901313T patent/DK0621766T3/da active
- 1993-11-15 EP EP94901313A patent/EP0621766B1/en not_active Expired - Lifetime
- 1993-11-15 JP JP6512252A patent/JPH08500041A/ja active Pending
- 1993-11-15 DE DE69330402T patent/DE69330402T2/de not_active Expired - Fee Related
- 1993-11-15 AT AT94901313T patent/ATE202688T1/de not_active IP Right Cessation
1994
- 1994-07-11 OA OA60536A patent/OA10089A/en unknown
2001
- 2001-10-03 GR GR20010401639T patent/GR3036778T3/el not_active IP Right Cessation

Also Published As

Publication number	Publication date
WO1994010940A1 (en)	1994-05-26
US5374515A (en)	1994-12-20
GR3036778T3 (en)	2002-01-31
CA2127120A1 (en)	1994-05-26
EP0621766B1 (en)	2001-07-04
ES2160116T3 (es)	2001-11-01
PT621766E (pt)	2001-12-28
ATE202688T1 (de)	2001-07-15
EP0621766A4 (en)	1996-04-24
EP0621766A1 (en)	1994-11-02
CN1091320A (zh)	1994-08-31
DE69330402D1 (de)	2001-08-09
JPH08500041A (ja)	1996-01-09
CN1080130C (zh)	2002-03-06
DE69330402T2 (de)	2002-05-29
DK0621766T3 (da)	2001-09-17

Publication	Publication Date	Title
EP0621766B1 (en)	2001-07-04	In vitro produced cornea equivalent model
US5827641A (en)	1998-10-27	In vitro cornea equivalent model
Zieske et al.	1994	Basement membrane assembly and differentiation of cultured corneal cells: importance of culture environment and endothelial cell interaction
Guo et al.	1989	Basement membrane and human epidermal differentiation in vitro
US6497875B1 (en)	2002-12-24	Multilayer skin or dermal equivalent having a layer containing mesenchymal stem cells
Compton et al.	1998	Organized skin structure is regenerated in vivo from collagen-GAG matrices seeded with autologous keratinocytes
Kinikoglu et al.	2009	Reconstruction of a full-thickness collagen-based human oral mucosal equivalent
CA2227827A1 (en)	1999-07-23	In vitro artificial cornea and sclera
JPH09504968A (ja)	1997-05-20	可溶性因子による上皮細胞の付着刺激及びヘミデスモソーム構成刺激
RU2498808C2 (ru)	2013-11-20	Способ лечения состояния ротовой полости больного (варианты)
US4888291A (en)	1989-12-19	Human epithelium originating from cell cultures
Strassburg et al.	2019	In vivo evaluation of an electrospun gelatin nonwoven mat for regeneration of epithelial tissues
Sweeney et al.	2003	A comparison of biological coatings for the promotion of corneal epithelialization of synthetic surface in vivo
Arita et al.	1993	Synthesis of lens capsule in long-term culture of human lens epithelial cells.
JP2015097524A (ja)	2015-05-28	再構成皮膚の作製用の組成物および方法
AU784221B2 (en)	2006-02-23	Improved keratinocyte culture and uses thereof
Sanmano et al.	2005	Engraftment of umbilical cord epithelial cells in athymic mice: in an attempt to improve reconstructed skin equivalents used as epithelial composite
US20210096127A1 (en)	2021-04-01	Means and methods for monitoring scar development
JP2008043772A (ja)	2008-02-28	皮膚移植片のための方法および組成物
MXPA97003321A (en)	1997-12-01	In vitro fabric and equivalen organ models
Alaminos Mingorance et al.	2006	Construction of a complete rabbit cornea substitute using a fibrin-agarose scaffold
CA2318641A1 (en)	1999-07-29	Artificial cornea
AU1832601A (en)	2001-04-26	Skin regeneration using mesenchymal stem cells