OA11997A - Streptomyces avermitillis gene directing the ratioof B2:B1 avermectins. - Google Patents

Streptomyces avermitillis gene directing the ratioof B2:B1 avermectins. Download PDF

Info

Publication number: OA11997A
Authority: OA; OAPI
Prior art keywords: leu; ala; val; gly; arg
Prior art date: 1999-08-12

Application number

OA1200200024A

Other languages

English (en)

Inventor

Kim Jonelle Stutzman-Engwall

Original Assignee

Pfizer Prod Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-08-12

Filing date

2000-07-19

Publication date

2006-04-18

2000-07-19 Application filed by Pfizer Prod Inc filed Critical Pfizer Prod Inc

2006-04-18 Publication of OA11997A publication Critical patent/OA11997A/en

Links

108090000623 proteins and genes Proteins 0.000 title claims abstract description 248
239000005660 Abamectin Substances 0.000 title claims abstract description 213
241000187747 Streptomyces Species 0.000 title claims description 17
241001468227 Streptomyces avermitilis Species 0.000 claims abstract description 239
239000013598 vector Substances 0.000 claims abstract description 59
108700028369 Alleles Proteins 0.000 claims description 123
230000035772 mutation Effects 0.000 claims description 88
235000001014 amino acid Nutrition 0.000 claims description 72
238000000034 method Methods 0.000 claims description 57
150000001413 amino acids Chemical class 0.000 claims description 52
238000004519 manufacturing process Methods 0.000 claims description 52
125000000539 amino acid group Chemical group 0.000 claims description 51
239000000203 mixture Substances 0.000 claims description 35
238000006467 substitution reaction Methods 0.000 claims description 34
241000187391 Streptomyces hygroscopicus Species 0.000 claims description 25
239000002609 medium Substances 0.000 claims description 22
125000000113 cyclohexyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])C1([H])[H] 0.000 claims description 21
125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 21
230000002829 reductive effect Effects 0.000 claims description 21
239000002253 acid Chemical group 0.000 claims description 14
AYFVYJQAPQTCCC-UHFFFAOYSA-N THREONINE Chemical compound CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims description 11
239000004473 Threonine Substances 0.000 claims description 10
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims description 9
235000004279 alanine Nutrition 0.000 claims description 8
239000001963 growth medium Substances 0.000 claims description 7
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 6
238000012258 culturing Methods 0.000 claims description 6
COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims description 5
241000970979 Streptomyces griseochromogenes Species 0.000 claims description 5
COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 claims description 5
241000282326 Felis catus Species 0.000 claims description 3
VPZXBVLAVMBEQI-UHFFFAOYSA-N glycyl-DL-alpha-alanine Natural products OC(=O)C(C)NC(=O)CN VPZXBVLAVMBEQI-UHFFFAOYSA-N 0.000 claims 15
108010079364 N-glycylalanine Proteins 0.000 claims 11
108010050848 glycylleucine Proteins 0.000 claims 9
241000880493 Leptailurus serval Species 0.000 claims 8
AJHCSUXXECOXOY-UHFFFAOYSA-N N-glycyl-L-tryptophan Natural products C1=CC=C2C(CC(NC(=O)CN)C(O)=O)=CNC2=C1 AJHCSUXXECOXOY-UHFFFAOYSA-N 0.000 claims 7
KFKWRHQBZQICHA-STQMWFEESA-N L-leucyl-L-phenylalanine Natural products CC(C)C[C@H](N)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 KFKWRHQBZQICHA-STQMWFEESA-N 0.000 claims 6
108010044056 leucyl-phenylalanine Proteins 0.000 claims 6
108010057821 leucylproline Proteins 0.000 claims 6
SGPGESCZOCHFCL-UHFFFAOYSA-N Tilisolol hydrochloride Chemical compound [Cl-].C1=CC=C2C(=O)N(C)C=C(OCC(O)C[NH2+]C(C)(C)C)C2=C1 SGPGESCZOCHFCL-UHFFFAOYSA-N 0.000 claims 5
AEFJNECXZCODJM-UWVGGRQHSA-N Val-Val-Gly Chemical compound CC(C)[C@H]([NH3+])C(=O)N[C@@H](C(C)C)C(=O)NCC([O-])=O AEFJNECXZCODJM-UWVGGRQHSA-N 0.000 claims 5
SMCGQGDVTPFXKB-XPUUQOCRSA-N Ala-Gly-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)CNC(=O)[C@H](C)N SMCGQGDVTPFXKB-XPUUQOCRSA-N 0.000 claims 4
XEPSCVXTCUUHDT-AVGNSLFASA-N Arg-Arg-Leu Natural products CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@@H](N)CCCN=C(N)N XEPSCVXTCUUHDT-AVGNSLFASA-N 0.000 claims 4
FKYWFUYPVKLJLP-DCAQKATOSA-N Ser-Pro-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CO FKYWFUYPVKLJLP-DCAQKATOSA-N 0.000 claims 4
108010027668 glycyl-alanyl-valine Proteins 0.000 claims 4
108010073025 phenylalanylphenylalanine Proteins 0.000 claims 4
108010029020 prolylglycine Proteins 0.000 claims 4
XVZCXCTYGHPNEM-IHRRRGAJSA-N (2s)-1-[(2s)-2-[[(2s)-2-amino-4-methylpentanoyl]amino]-4-methylpentanoyl]pyrrolidine-2-carboxylic acid Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(O)=O XVZCXCTYGHPNEM-IHRRRGAJSA-N 0.000 claims 3
PKOHVHWNGUHYRE-ZFWWWQNUSA-N (2s)-1-[2-[[(2s)-2-amino-3-(1h-indol-3-yl)propanoyl]amino]acetyl]pyrrolidine-2-carboxylic acid Chemical compound O=C([C@H](CC=1C2=CC=CC=C2NC=1)N)NCC(=O)N1CCC[C@H]1C(O)=O PKOHVHWNGUHYRE-ZFWWWQNUSA-N 0.000 claims 3
MNZHHDPWDWQJCQ-YUMQZZPRSA-N Ala-Leu-Gly Chemical compound C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)NCC(O)=O MNZHHDPWDWQJCQ-YUMQZZPRSA-N 0.000 claims 3
OYJCVIGKMXUVKB-GARJFASQSA-N Ala-Leu-Pro Chemical compound C[C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@@H]1C(=O)O)N OYJCVIGKMXUVKB-GARJFASQSA-N 0.000 claims 3
HOVPGJUNRLMIOZ-CIUDSAMLSA-N Ala-Ser-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CO)NC(=O)[C@H](C)N HOVPGJUNRLMIOZ-CIUDSAMLSA-N 0.000 claims 3
AETQNIIFKCMVHP-UVBJJODRSA-N Ala-Trp-Arg Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O AETQNIIFKCMVHP-UVBJJODRSA-N 0.000 claims 3
QHVRVUNEAIFTEK-SZMVWBNQSA-N Arg-Pro-Trp Chemical compound N[C@@H](CCCNC(N)=N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(O)=O QHVRVUNEAIFTEK-SZMVWBNQSA-N 0.000 claims 3
UGVQELHRNUDMAA-BYPYZUCNSA-N Gly-Ala-Gly Chemical compound [NH3+]CC(=O)N[C@@H](C)C(=O)NCC([O-])=O UGVQELHRNUDMAA-BYPYZUCNSA-N 0.000 claims 3
UHPAZODVFFYEEL-QWRGUYRKSA-N Gly-Leu-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)CN UHPAZODVFFYEEL-QWRGUYRKSA-N 0.000 claims 3
SITWEMZOJNKJCH-UHFFFAOYSA-N L-alanine-L-arginine Natural products CC(N)C(=O)NC(C(O)=O)CCCNC(N)=N SITWEMZOJNKJCH-UHFFFAOYSA-N 0.000 claims 3
TYYLDKGBCJGJGW-UHFFFAOYSA-N L-tryptophan-L-tyrosine Natural products C=1NC2=CC=CC=C2C=1CC(N)C(=O)NC(C(O)=O)CC1=CC=C(O)C=C1 TYYLDKGBCJGJGW-UHFFFAOYSA-N 0.000 claims 3
BQSLGJHIAGOZCD-CIUDSAMLSA-N Leu-Ala-Ser Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(O)=O BQSLGJHIAGOZCD-CIUDSAMLSA-N 0.000 claims 3
HYIFFZAQXPUEAU-QWRGUYRKSA-N Leu-Gly-Leu Chemical compound CC(C)C[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CC(C)C HYIFFZAQXPUEAU-QWRGUYRKSA-N 0.000 claims 3
XVZCXCTYGHPNEM-UHFFFAOYSA-N Leu-Leu-Pro Natural products CC(C)CC(N)C(=O)NC(CC(C)C)C(=O)N1CCCC1C(O)=O XVZCXCTYGHPNEM-UHFFFAOYSA-N 0.000 claims 3
KZNQNBZMBZJQJO-UHFFFAOYSA-N N-glycyl-L-proline Natural products NCC(=O)N1CCCC1C(O)=O KZNQNBZMBZJQJO-UHFFFAOYSA-N 0.000 claims 3
KXUZHWXENMYOHC-QEJZJMRPSA-N Phe-Leu-Ala Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(O)=O KXUZHWXENMYOHC-QEJZJMRPSA-N 0.000 claims 3
OBKOPLHSRDATFO-XHSDSOJGSA-N Tyr-Val-Pro Chemical compound CC(C)[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CC2=CC=C(C=C2)O)N OBKOPLHSRDATFO-XHSDSOJGSA-N 0.000 claims 3
108010077515 glycylproline Proteins 0.000 claims 3
108010079317 prolyl-tyrosine Proteins 0.000 claims 3
108010090894 prolylleucine Proteins 0.000 claims 3
108010044292 tryptophyltyrosine Proteins 0.000 claims 3
KIUYPHAMDKDICO-WHFBIAKZSA-N Ala-Asp-Gly Chemical compound C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)NCC(O)=O KIUYPHAMDKDICO-WHFBIAKZSA-N 0.000 claims 2
HXNNRBHASOSVPG-GUBZILKMSA-N Ala-Glu-Leu Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O HXNNRBHASOSVPG-GUBZILKMSA-N 0.000 claims 2
NIZKGBJVCMRDKO-KWQFWETISA-N Ala-Gly-Tyr Chemical compound C[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 NIZKGBJVCMRDKO-KWQFWETISA-N 0.000 claims 2
OKEWAFFWMHBGPT-XPUUQOCRSA-N Ala-His-Gly Chemical compound OC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](N)C)CC1=CN=CN1 OKEWAFFWMHBGPT-XPUUQOCRSA-N 0.000 claims 2
JEPNLGMEZMCFEX-QSFUFRPTSA-N Ala-His-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](CC1=CN=CN1)NC(=O)[C@H](C)N JEPNLGMEZMCFEX-QSFUFRPTSA-N 0.000 claims 2
IPZQNYYAYVRKKK-FXQIFTODSA-N Ala-Pro-Ala Chemical compound C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(O)=O IPZQNYYAYVRKKK-FXQIFTODSA-N 0.000 claims 2
OLVCTPPSXNRGKV-GUBZILKMSA-N Ala-Pro-Pro Chemical compound C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N1[C@H](C(O)=O)CCC1 OLVCTPPSXNRGKV-GUBZILKMSA-N 0.000 claims 2
DCVYRWFAMZFSDA-ZLUOBGJFSA-N Ala-Ser-Ala Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(O)=O DCVYRWFAMZFSDA-ZLUOBGJFSA-N 0.000 claims 2
ARHJJAAWNWOACN-FXQIFTODSA-N Ala-Ser-Val Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(O)=O ARHJJAAWNWOACN-FXQIFTODSA-N 0.000 claims 2
IOFVWPYSRSCWHI-JXUBOQSCSA-N Ala-Thr-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](C)N IOFVWPYSRSCWHI-JXUBOQSCSA-N 0.000 claims 2
BOKLLPVAQDSLHC-FXQIFTODSA-N Ala-Val-Cys Chemical compound C[C@@H](C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CS)C(=O)O)N BOKLLPVAQDSLHC-FXQIFTODSA-N 0.000 claims 2
VHAQSYHSDKERBS-XPUUQOCRSA-N Ala-Val-Gly Chemical compound C[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)NCC(O)=O VHAQSYHSDKERBS-XPUUQOCRSA-N 0.000 claims 2
KJGNDQCYBNBXDA-GUBZILKMSA-N Arg-Arg-Cys Chemical compound C(C[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CS)C(=O)O)N)CN=C(N)N KJGNDQCYBNBXDA-GUBZILKMSA-N 0.000 claims 2
OVVUNXXROOFSIM-SDDRHHMPSA-N Arg-Arg-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CCCN=C(N)N)N)C(=O)O OVVUNXXROOFSIM-SDDRHHMPSA-N 0.000 claims 2
HJAICMSAKODKRF-GUBZILKMSA-N Arg-Cys-Arg Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O HJAICMSAKODKRF-GUBZILKMSA-N 0.000 claims 2
CYXCAHZVPFREJD-LURJTMIESA-N Arg-Gly-Gly Chemical compound NC(=N)NCCC[C@H](N)C(=O)NCC(=O)NCC(O)=O CYXCAHZVPFREJD-LURJTMIESA-N 0.000 claims 2
IRRMIGDCPOPZJW-ULQDDVLXSA-N Arg-His-Phe Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O IRRMIGDCPOPZJW-ULQDDVLXSA-N 0.000 claims 2
GMRGSBAMMMVDGG-GUBZILKMSA-N Asn-Arg-Arg Chemical compound C(C[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)O)NC(=O)[C@H](CC(=O)N)N)CN=C(N)N GMRGSBAMMMVDGG-GUBZILKMSA-N 0.000 claims 2
SEKBHZJLARBNPB-GHCJXIJMSA-N Asn-Ile-Ser Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(O)=O SEKBHZJLARBNPB-GHCJXIJMSA-N 0.000 claims 2
CPYHLXSGDBDULY-IHPCNDPISA-N Asn-Trp-Phe Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O CPYHLXSGDBDULY-IHPCNDPISA-N 0.000 claims 2
IXIWEFWRKIUMQX-DCAQKATOSA-N Asp-Arg-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@@H](N)CC(O)=O IXIWEFWRKIUMQX-DCAQKATOSA-N 0.000 claims 2
KGHLGJAXYSVNJP-WHFBIAKZSA-N Asp-Ser-Gly Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](CO)C(=O)NCC(O)=O KGHLGJAXYSVNJP-WHFBIAKZSA-N 0.000 claims 2
GIKOVDMXBAFXDF-NHCYSSNCSA-N Asp-Val-Leu Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O GIKOVDMXBAFXDF-NHCYSSNCSA-N 0.000 claims 2
XLLSMEFANRROJE-GUBZILKMSA-N Cys-Leu-Glu Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)O)NC(=O)[C@H](CS)N XLLSMEFANRROJE-GUBZILKMSA-N 0.000 claims 2
OPAINBJQDQTGJY-JGVFFNPUSA-N Glu-Gly-Pro Chemical compound C1C[C@@H](N(C1)C(=O)CNC(=O)[C@H](CCC(=O)O)N)C(=O)O OPAINBJQDQTGJY-JGVFFNPUSA-N 0.000 claims 2
OQXDUSZKISQQSS-GUBZILKMSA-N Glu-Lys-Ala Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(O)=O OQXDUSZKISQQSS-GUBZILKMSA-N 0.000 claims 2
WGYHAAXZWPEBDQ-IFFSRLJSSA-N Glu-Val-Thr Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O WGYHAAXZWPEBDQ-IFFSRLJSSA-N 0.000 claims 2
PYTZFYUXZZHOAD-WHFBIAKZSA-N Gly-Ala-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)CN PYTZFYUXZZHOAD-WHFBIAKZSA-N 0.000 claims 2
LERGJIVJIIODPZ-ZANVPECISA-N Gly-Ala-Trp Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@@H](NC(=O)CN)C)C(O)=O)=CNC2=C1 LERGJIVJIIODPZ-ZANVPECISA-N 0.000 claims 2
LCNXZQROPKFGQK-WHFBIAKZSA-N Gly-Asp-Ser Chemical compound NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(O)=O LCNXZQROPKFGQK-WHFBIAKZSA-N 0.000 claims 2
MBOAPAXLTUSMQI-JHEQGTHGSA-N Gly-Glu-Thr Chemical compound [H]NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O MBOAPAXLTUSMQI-JHEQGTHGSA-N 0.000 claims 2
PAWIVEIWWYGBAM-YUMQZZPRSA-N Gly-Leu-Ala Chemical compound NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(O)=O PAWIVEIWWYGBAM-YUMQZZPRSA-N 0.000 claims 2
JNGHLWWFPGIJER-STQMWFEESA-N Gly-Pro-Tyr Chemical compound NCC(=O)N1CCC[C@H]1C(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 JNGHLWWFPGIJER-STQMWFEESA-N 0.000 claims 2
YDIDLLVFCYSXNY-RCOVLWMOSA-N Gly-Val-Asn Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)CN YDIDLLVFCYSXNY-RCOVLWMOSA-N 0.000 claims 2
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CNMOKANDJMLAIF-CIQUZCHMSA-N Ile-Thr-Ala Chemical compound CC[C@H](C)[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C)C(O)=O CNMOKANDJMLAIF-CIQUZCHMSA-N 0.000 claims 2
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BMVFXOQHDQZAQU-DCAQKATOSA-N Leu-Pro-Asp Chemical compound CC(C)C[C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(=O)O)C(=O)O)N BMVFXOQHDQZAQU-DCAQKATOSA-N 0.000 claims 2
WBRJVRXEGQIDRK-XIRDDKMYSA-N Leu-Trp-Ser Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@@H](N)CC(C)C)C(=O)N[C@@H](CO)C(O)=O)=CNC2=C1 WBRJVRXEGQIDRK-XIRDDKMYSA-N 0.000 claims 2
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239000003826 tablet Substances 0.000 description 1
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239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 1
150000007970 thio esters Chemical class 0.000 description 1
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108010050327 trypticase-soy broth Proteins 0.000 description 1
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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
- C12P17/181—Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
- C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
- C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
- C12P19/623—Avermectin; Milbemycin; Ivermectin; C-076

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Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biomedical Technology (AREA)
Chemical Kinetics & Catalysis (AREA)
Molecular Biology (AREA)
General Chemical & Material Sciences (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Medicinal Chemistry (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Agricultural Chemicals And Associated Chemicals (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

OA1200200024A 1999-08-12 2000-07-19 Streptomyces avermitillis gene directing the ratioof B2:B1 avermectins. OA11997A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US09/372,934 US6248579B1 (en)	1998-02-13	1999-08-12	Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins

Publications (1)

Publication Number	Publication Date
OA11997A true OA11997A (en)	2006-04-18

Family

ID=23470234

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
OA1200200024A OA11997A (en)	1999-08-12	2000-07-19	Streptomyces avermitillis gene directing the ratioof B2:B1 avermectins.

Country Status (37)

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US (8)	US6248579B1 (de)
EP (1)	EP1200605B1 (de)
JP (2)	JP3688640B2 (de)
KR (1)	KR100486006B1 (de)
CN (1)	CN1186447C (de)
AP (2)	AP2002002417A0 (de)
AR (1)	AR021450A1 (de)
AT (1)	ATE283363T1 (de)
AU (1)	AU779756B2 (de)
BG (1)	BG106478A (de)
BR (1)	BR0013093A (de)
CA (1)	CA2381427A1 (de)
CO (1)	CO5290296A1 (de)
CZ (1)	CZ2002297A3 (de)
DE (1)	DE60016231T2 (de)
EA (1)	EA004569B1 (de)
ES (1)	ES2231214T3 (de)
GT (2)	GT200000138A (de)
HK (1)	HK1046536B (de)
HR (1)	HRP20020131A2 (de)
HU (1)	HUP0202517A2 (de)
IL (1)	IL147562A0 (de)
IS (1)	IS6249A (de)
MA (1)	MA26811A1 (de)
MX (1)	MXPA02001514A (de)
NO (1)	NO20020665L (de)
OA (1)	OA11997A (de)
PA (1)	PA8499201A1 (de)
PE (1)	PE20010697A1 (de)
PL (1)	PL353770A1 (de)
PT (1)	PT1200605E (de)
SK (1)	SK1382002A3 (de)
TN (1)	TNSN00170A1 (de)
UY (1)	UY26287A1 (de)
WO (1)	WO2001012822A1 (de)
YU (1)	YU8402A (de)
ZA (1)	ZA200201071B (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
BRPI0013119B8 (pt) *	1999-08-12	2021-05-25	Pah Usa 15 Llc	molécula polinucleotídica isolada, vetor recombinante purificado, célula hospedeira procariótica e célula de streptomyces avermitilis mutada, método para obtenção de uma nova cepa de s. avermitilis e processo para produção de avermectinas
JP2005516987A (ja)	2002-02-05	2005-06-09	ブリストル−マイヤーズ　スクイブ　カンパニー	Ｎ−置換された３−ヒドロキシ−４−ピリジノン化合物およびそれを含む医薬
PT1476539E (pt)	2002-02-12	2009-04-09	Pfizer Prod Inc	Gene streptomyces avermitilis que dirige a proporção de avermectinas b2:b1
US7943160B2 (en) *	2002-05-09	2011-05-17	Scimetrics Limited Corp.	Pest control methods
US8440609B2 (en) *	2003-01-31	2013-05-14	Gerd Wallukat	Peptides against autoantibodies causing intolerance to cold and use thereof
KR100759130B1 (ko)	2005-02-12	2007-09-19	휴메드 주식회사	항 인테그린 항체 코팅스텐트 및 그의 제조방법
CN106148216B (zh)	2015-03-27	2019-06-04	浙江海正药业股份有限公司	一种链霉菌及其生产米尔贝霉素a3的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5234831A (en)	1987-01-23	1993-08-10	Pfizer Inc	Cultures for production of B avermectins
US5525506A (en)	1987-01-23	1996-06-11	Pfizer Inc.	Process for production of avermectins and cultures therefor
US5238848A (en)	1987-01-23	1993-08-24	Pfizer Inc	Cultures for production of avermectins
DE3881147T2 (de)	1987-10-23	1993-09-02	Pfizer	Verfahren zur herstellung von aglykonen von avermectin und sie enthaltende kulturen.
US5240850A (en)	1987-10-23	1993-08-31	Pfizer Inc.	Cultures for production of avermectin aglycones
ES2079436T3 (es)	1989-03-31	1996-01-16	Merck & Co Inc	Clonacion de genes de streptomyces avermitilis para biosintesis de avermectinas y procedimientos para su uso.
US5252474A (en)	1989-03-31	1993-10-12	Merck & Co., Inc.	Cloning genes from Streptomyces avermitilis for avermectin biosynthesis and the methods for their use
JP2807348B2 (ja)	1993-07-30	1998-10-08	ファイザー・インコーポレーテッド	ストレプトミセス・アベルミティリスからの分岐状鎖α−ケト酸デヒドロゲナーゼ複合体をコードする遺伝子
RU2120943C1 (ru) *	1993-10-05	1998-10-27	Пфайзер Инк.	Производные дорамектина, способы их получения, способы лечения
FI942725L (fi)	1993-12-16	1995-06-17	Pfizer	Haaraketjuista alfaketohappodenydrogenaasikompleksia koodaavia geenejä Streptomyces avermitiliksesta
CN1521180A (zh) *	1998-02-13	2004-08-18	�Ʒ�	介导除虫菌素b2：b1比例的除虫链霉菌基因

1999
- 1999-08-12 US US09/372,934 patent/US6248579B1/en not_active Expired - Fee Related
2000
- 2000-07-19 MX MXPA02001514A patent/MXPA02001514A/es active IP Right Grant
- 2000-07-19 HU HU0202517A patent/HUP0202517A2/hu unknown
- 2000-07-19 HR HR20020131A patent/HRP20020131A2/hr not_active Application Discontinuation
- 2000-07-19 HK HK02107834.3A patent/HK1046536B/zh not_active IP Right Cessation
- 2000-07-19 AU AU57021/00A patent/AU779756B2/en not_active Ceased
- 2000-07-19 YU YU8402A patent/YU8402A/sh unknown
- 2000-07-19 AP APAP/P/2002/002417A patent/AP2002002417A0/en unknown
- 2000-07-19 BR BR0013093-1A patent/BR0013093A/pt not_active Application Discontinuation
- 2000-07-19 DE DE60016231T patent/DE60016231T2/de not_active Expired - Fee Related
- 2000-07-19 IL IL14756200A patent/IL147562A0/xx unknown
- 2000-07-19 EA EA200200057A patent/EA004569B1/ru not_active IP Right Cessation
- 2000-07-19 AP APAP/P/2002/002419A patent/AP2002002419A0/en unknown
- 2000-07-19 CZ CZ2002297A patent/CZ2002297A3/cs unknown
- 2000-07-19 CN CNB008117632A patent/CN1186447C/zh not_active Expired - Fee Related
- 2000-07-19 WO PCT/IB2000/000996 patent/WO2001012822A1/en not_active Ceased
- 2000-07-19 PT PT00942331T patent/PT1200605E/pt unknown
- 2000-07-19 OA OA1200200024A patent/OA11997A/en unknown
- 2000-07-19 KR KR10-2002-7001946A patent/KR100486006B1/ko not_active Expired - Fee Related
- 2000-07-19 CA CA002381427A patent/CA2381427A1/en not_active Abandoned
- 2000-07-19 EP EP00942331A patent/EP1200605B1/de not_active Expired - Lifetime
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- 2000-07-19 AT AT00942331T patent/ATE283363T1/de not_active IP Right Cessation
- 2000-08-04 PA PA20008499201A patent/PA8499201A1/es unknown
- 2000-08-04 CO CO00058850A patent/CO5290296A1/es not_active Application Discontinuation
- 2000-08-10 UY UY26287A patent/UY26287A1/es not_active Application Discontinuation
- 2000-08-10 PE PE2000000813A patent/PE20010697A1/es not_active Application Discontinuation
- 2000-08-10 AR ARP000104129A patent/AR021450A1/es unknown
- 2000-08-11 GT GT200000138A patent/GT200000138A/es unknown
- 2000-08-11 GT GT200000138CM patent/GT200000138CA/es unknown
- 2000-08-11 TN TNTNSN00170A patent/TNSN00170A1/fr unknown
2001
- 2001-01-22 US US09/766,898 patent/US6511841B2/en not_active Expired - Fee Related
- 2001-01-22 US US09/766,916 patent/US6509159B2/en not_active Expired - Fee Related
2002
- 2002-01-25 IS IS6249A patent/IS6249A/is unknown
- 2002-02-07 MA MA26511A patent/MA26811A1/fr unknown
- 2002-02-07 ZA ZA200201071A patent/ZA200201071B/en unknown
- 2002-02-11 NO NO20020665A patent/NO20020665L/no not_active Application Discontinuation
- 2002-03-05 BG BG106478A patent/BG106478A/bg unknown
- 2002-11-27 US US10/306,246 patent/US20030129635A1/en not_active Abandoned
- 2002-11-27 US US10/306,013 patent/US20030134313A1/en not_active Abandoned
- 2002-11-27 US US10/306,249 patent/US6833263B2/en not_active Expired - Fee Related
- 2002-11-27 US US10/306,247 patent/US7029887B2/en not_active Expired - Fee Related
2004
- 2004-12-14 US US11/011,960 patent/US7259241B2/en not_active Expired - Fee Related
2005
- 2005-02-16 JP JP2005039041A patent/JP2005198658A/ja not_active Withdrawn

Also Published As

Publication number	Publication date
HK1046536A1 (en)	2003-01-17
DE60016231T2 (de)	2005-12-08
US20050196777A1 (en)	2005-09-08
ES2231214T3 (es)	2005-05-16
AU779756B2 (en)	2005-02-10
CN1186447C (zh)	2005-01-26
AP2002002419A0 (en)	2002-03-31
NO20020665D0 (no)	2002-02-11
US7029887B2 (en)	2006-04-18
KR100486006B1 (ko)	2005-05-03
US20010016350A1 (en)	2001-08-23
HRP20020131A2 (en)	2003-12-31
AR021450A1 (es)	2002-07-17
UY26287A1 (es)	2001-03-16
HK1046536B (zh)	2005-05-13
US20030134313A1 (en)	2003-07-17
CA2381427A1 (en)	2001-02-22
JP3688640B2 (ja)	2005-08-31
EP1200605B1 (de)	2004-11-24
CO5290296A1 (es)	2003-06-27
US20030129636A1 (en)	2003-07-10
PE20010697A1 (es)	2001-07-05
GT200000138A (es)	2002-02-02
US6833263B2 (en)	2004-12-21
PT1200605E (pt)	2005-04-29
PA8499201A1 (es)	2001-12-14
AP2002002417A0 (en)	2002-03-31
EA004569B1 (ru)	2004-06-24
US6248579B1 (en)	2001-06-19
IS6249A (is)	2002-01-25
BR0013093A (pt)	2002-08-13
IL147562A0 (en)	2002-08-14
US6509159B2 (en)	2003-01-21
US6511841B2 (en)	2003-01-28
TNSN00170A1 (fr)	2005-11-10
HUP0202517A2 (hu)	2002-11-28
US20030124607A1 (en)	2003-07-03
JP2005198658A (ja)	2005-07-28
JP2003507018A (ja)	2003-02-25
CN1370239A (zh)	2002-09-18
ATE283363T1 (de)	2004-12-15
US7259241B2 (en)	2007-08-21
DE60016231D1 (de)	2004-12-30
KR20020022809A (ko)	2002-03-27
WO2001012822A1 (en)	2001-02-22
EA200200057A1 (ru)	2002-06-27
EP1200605A1 (de)	2002-05-02
SK1382002A3 (en)	2002-11-06
MA26811A1 (fr)	2004-12-20
MXPA02001514A (es)	2002-07-02
NO20020665L (no)	2002-02-11
BG106478A (bg)	2004-02-27
GT200000138CA (es)	2002-02-02
ZA200201071B (en)	2003-06-25
AU5702100A (en)	2001-03-13
CZ2002297A3 (cs)	2002-08-14
PL353770A1 (en)	2003-12-01
US20010024818A1 (en)	2001-09-27
YU8402A (sh)	2006-03-03
US20030129635A1 (en)	2003-07-10

Publication	Publication Date	Title
US6509159B2 (en)	2003-01-21	Methods related to Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
AU752343C (en)	2005-11-03	Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
CA2380872C (en)	2007-11-27	Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins
MXPA04007777A (es)	2004-10-15	Gen de streptomyces avertmitilis que rige la relacion de avermectinas b2:b1.
AU779569B2 (en)	2005-01-27	Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
MXPA00007915A (en)	2001-07-09	I(streptomyces avermitilis) gene directing the ratio of b2:b1 avermectins