PE20020003A1 - Purificacion de cuerpos de inclusion de proteinas por microfiltracion de flujo cruzado - Google Patents

Purificacion de cuerpos de inclusion de proteinas por microfiltracion de flujo cruzado

Info

Publication number
PE20020003A1
PE20020003A1 PE2001000410A PE2001000410A PE20020003A1 PE 20020003 A1 PE20020003 A1 PE 20020003A1 PE 2001000410 A PE2001000410 A PE 2001000410A PE 2001000410 A PE2001000410 A PE 2001000410A PE 20020003 A1 PE20020003 A1 PE 20020003A1
Authority
PE
Peru
Prior art keywords
bodies
semi
inclusion bodies
protein inclusion
purification
Prior art date
Application number
PE2001000410A
Other languages
English (en)
Inventor
Jorg Peters
Original Assignee
Bayer Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Ag filed Critical Bayer Ag
Publication of PE20020003A1 publication Critical patent/PE20020003A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Water Supply & Treatment (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

DONDE LA SOLUCION QUE CONTIENE DICHOS CUERPOS, SE HACE PASAR TANGENCIALMENTE A UNA O VARIAS MEMBRANAS SEMIPERMEABLES, DE MODO QUE DICHOS CUERPOS SON RECHAZADOS POR LAS MEMBRANAS Y LAS SUSTANCIAS O COMPONENTES DE MENOR PESO MOLECULAR O DIAMETRO DE PARTICULA PUEDEN PASAR Y QUEDAR ABSORBIDAS EN LA MEMBRANA, OBTENIENDOSE UNA SOLUCION DE CUERPOS DE INCLUSION DE PROTEINAS PURIFICADA Y/O CONCENTRADA, ENCONTRANDOSE SOBRE LA MEMBRANA SEMIPERMEABLE UNA MATRIZ DE MALLA ABIERTA QUE ESTA ESTRUCTURADA POR HILOS LONGITUDINALES Y TRANSVERSALES QUE SE CRUZAN DE MODO QUE LOS HILOS CONTIGUOS ESTAN DISTANCIADOS RESPECTIVAMENTE ENTRE SI DE 5 A 15 VECES SU ESPESOR QUE ES DE 150 µm A 600 µm. LA CARGA DE BIOMASA DE LA MEMBRANA ES MAYOR DE 65 g DE EQUIVALENTE DE PESO HUMEDO CELULAR/m2
PE2001000410A 2000-05-08 2001-05-08 Purificacion de cuerpos de inclusion de proteinas por microfiltracion de flujo cruzado PE20020003A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10022258A DE10022258A1 (de) 2000-05-08 2000-05-08 Reinigung von Proteineinschlusskörpern durch Querstrom-Mikrofiltration

Publications (1)

Publication Number Publication Date
PE20020003A1 true PE20020003A1 (es) 2002-03-28

Family

ID=7641110

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2001000410A PE20020003A1 (es) 2000-05-08 2001-05-08 Purificacion de cuerpos de inclusion de proteinas por microfiltracion de flujo cruzado

Country Status (14)

Country Link
US (1) US6936699B2 (es)
EP (1) EP1282637B1 (es)
JP (1) JP4818561B2 (es)
AR (1) AR028392A1 (es)
AU (1) AU2001272378A1 (es)
CA (1) CA2408902C (es)
DE (2) DE10022258A1 (es)
DO (1) DOP2001000159A (es)
ES (1) ES2215916T3 (es)
MY (1) MY126838A (es)
PE (1) PE20020003A1 (es)
SV (1) SV2002000438A (es)
TW (1) TWI248940B (es)
WO (1) WO2001085757A2 (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10237082B4 (de) * 2002-08-09 2014-12-31 Sartorius Stedim Biotech Gmbh Verfahren und Vorrichtung zur biotechnologischen Herstellung von Wertstoffen
US20070207461A1 (en) * 2004-02-23 2007-09-06 Crucell Holland B.V. Virus Purification Methods
CA2602944C (en) * 2005-04-11 2015-08-11 Crucell Holland B.V. Virus purification using ultrafiltration
EP2174140B1 (en) 2007-07-03 2016-01-13 Amgen Inc. Calculation of protein concentration using inclusion body dry weight
US8535536B1 (en) 2009-07-04 2013-09-17 University Of Utah Research Foundation Cross-flow split-thin-flow cell
SG194779A1 (en) 2011-05-04 2013-12-30 Univ Cornell Multiblock copolymer films, methods of making same, and uses thereof
CA2857307A1 (en) * 2011-12-01 2013-06-06 Barofold, Inc. Methods and systems for protein refolding
SG11201600380VA (en) * 2013-07-19 2016-02-26 Biogenomics Ltd Apparatus for refolding of recombinant proteins
JP2015065942A (ja) * 2013-09-30 2015-04-13 富士フイルム株式会社 細胞培養担体および細胞培養容器
KR101663036B1 (ko) 2014-07-11 2016-10-06 한국과학기술원 미세조류 농축 및 수소 생성 장치
EP3221687B1 (en) * 2014-11-17 2019-05-01 Syddansk Universitet Assembly for assessing drug permeability with adjustable biomimetic properties
WO2017189697A1 (en) 2016-04-28 2017-11-02 Terapore Technologies, Inc. Charged isoporous materials for electrostatic separations
MX2019005774A (es) 2016-11-17 2020-02-07 Terapore Tech Inc Películas isoporosas de copolímero de bloques autoensamblados que contienen aditivos hidrofílicos de alto peso molecular y métodos para hacer las mismas.
WO2018156731A1 (en) * 2017-02-22 2018-08-30 Dorin Rachel M Ligand bound mbp membranes, uses and method of manufacturing
CA3062637A1 (en) 2017-05-12 2018-11-15 Terapore Technologies, Inc. Chemically resistant fluorinated multiblock polymer structures, methods of manufacturing and use
CN111107924B (zh) 2017-09-19 2022-12-27 特拉波雷技术有限公司 耐化学品的均孔交联嵌段共聚物结构
MX2020009506A (es) 2018-03-12 2021-01-15 Terapore Tech Inc Materiales de copolímero de bloques asimétricos mesoporosos isoporosos con macrohuecos y método para fabricarlo.
CN112452157B (zh) * 2020-11-26 2023-05-26 济南磐升生物技术有限公司 一种胶原蛋白提取纯化装置

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4137333A1 (de) 1991-11-13 1993-05-19 W Prof Dr Sebald Therapeutische mittel, die antagonisten oder partielle agonisten des humanen interleukin 4 sind oder diese enthalten, hil-4-mutantenproteine sowie vefahren zu deren herstellung
US5616595A (en) * 1995-06-07 1997-04-01 Abbott Laboratories Process for recovering water insoluble compounds from a fermentation broth
EP0853123A1 (de) * 1997-01-10 1998-07-15 Roche Diagnostics GmbH Reinigung von DNA durch Cross-Flow-Filtration
EP1022337B1 (en) 1999-01-20 2006-07-26 Bayer HealthCare AG Plasmids, their construction and their use in the manufacture of interleukin-4 and interleukin-4 muteins
DE69929638T2 (de) 1999-01-20 2006-09-21 Bayer Healthcare Ag Plasmide, deren Konstruktion und Anwendung zur Herstellung von Interleukin-4 und dessen Muteine

Also Published As

Publication number Publication date
TWI248940B (en) 2006-02-11
US20030171560A1 (en) 2003-09-11
AR028392A1 (es) 2003-05-07
DE10022258A1 (de) 2001-11-15
ES2215916T3 (es) 2004-10-16
MY126838A (en) 2006-10-31
AU2001272378A1 (en) 2001-11-20
DE50101661D1 (de) 2004-04-15
DOP2001000159A (es) 2002-05-15
JP4818561B2 (ja) 2011-11-16
CA2408902A1 (en) 2002-11-05
CA2408902C (en) 2010-09-07
WO2001085757A2 (de) 2001-11-15
EP1282637B1 (de) 2004-03-10
EP1282637A2 (de) 2003-02-12
JP2003535582A (ja) 2003-12-02
SV2002000438A (es) 2002-07-03
US6936699B2 (en) 2005-08-30
WO2001085757A3 (de) 2002-03-28

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FD Application declared void or lapsed