SV2002000438A - Purificacion de cuerpos de inclusion de proteinas por microfiltracion de flujo cruzado ref. lea 33419-sv - Google Patents
Purificacion de cuerpos de inclusion de proteinas por microfiltracion de flujo cruzado ref. lea 33419-svInfo
- Publication number
- SV2002000438A SV2002000438A SV2001000438A SV2001000438A SV2002000438A SV 2002000438 A SV2002000438 A SV 2002000438A SV 2001000438 A SV2001000438 A SV 2001000438A SV 2001000438 A SV2001000438 A SV 2001000438A SV 2002000438 A SV2002000438 A SV 2002000438A
- Authority
- SV
- El Salvador
- Prior art keywords
- inclusion bodies
- protein inclusion
- purification
- suspension
- separation
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 10
- 108090000623 proteins and genes Proteins 0.000 title abstract 10
- 210000003000 inclusion body Anatomy 0.000 title abstract 9
- 238000000746 purification Methods 0.000 title abstract 4
- 238000011968 cross flow microfiltration Methods 0.000 title 1
- 239000000725 suspension Substances 0.000 abstract 5
- 239000002245 particle Substances 0.000 abstract 4
- 210000002421 cell wall Anatomy 0.000 abstract 3
- 239000012528 membrane Substances 0.000 abstract 3
- 238000000926 separation method Methods 0.000 abstract 3
- 238000002360 preparation method Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 1
- 238000001471 micro-filtration Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Water Supply & Treatment (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
LA INVENCION SE REFIERE A UN PROCEDIMIENTO PARA LA PURIFICACION Y/O CONCENTRACION DE CUERPOS DE INCLUSION DE PROTEINAS (EN INGLES "INCLUSION BODIES" O "REFLACTILE BODIES")EN UNA SOLUCION ALTAMENTE CONCENTRADA QUE CONTIENE PARTICULAS, EN EL QUE LA SUSPENSION QUE CONTIENE LOS CUERPOS DE INCLUSION DE PROTEINAS SE HACE PASAR TANGENCIALMENTE A UNA O VARIAS MEMBRANAS SEMIPERMEABLES, DE MODO QUE LOS CUERPOS DE INCLUSION DE PROTEINAS QUEDAN RECHAZADOS DETRAS DE LAS MEMBRANAS Y LAS SUBSTANCIAS DE MENOR PESO MOLECULAR PUEDEN PASAR A TRAVES DE LA MEMBRANA Y/O QUEDAN ABSORBIDAS EN LA MEMBRANA Y SE OBTIENE UNA SUSPENSION DE CUERPOS DE INCLUSION DE PROTEINAS PURIFICADA Y/O CONCENTRADA. DEL MISMO MODO SE PROCEDE PARA LA SEPARACION DE PARTICULAS DE PARED CELULAR DE UNA SUSPENSION DE CUERPOS DE INCLUSION DE PROTEINAS. ADEMAS, LA INVENCION SE REFIERE AL USO DE UNA INSTALACION DE MICROFILTRACION DE FLUJO CRUZADO PARA LA PURIFICACION Y/O CONCENTRACION DE CUERPOS DE INCLUSION DE PROTEINAS EN UNA SUSPENSION ASI COMO PARA LA SEPARACION DE PARTICULAS DE PARED CELULAR DE UNA SUSPENSION DE CUERPOS DE INCLUSION DE PROTEINAS, ADEMAS LA INVENCION SE REFIERE AL USO DE UNA INSTALACION DE MICCROFILTRACION DE FLUJO CRUZADO PARA LA PURIFICACION Y/O CONCENTRACION DE CUERPOS DE INCLUSION DE PROTEINAS EN UNA SUSPENSION ASI COMO PARA LA SEPARACION DE PARTICULAS DE PARED CELULAR DE UNA PREPARACION DE CUERPOS DE INCLUSION DE PROTEINAS.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10022258A DE10022258A1 (de) | 2000-05-08 | 2000-05-08 | Reinigung von Proteineinschlusskörpern durch Querstrom-Mikrofiltration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SV2002000438A true SV2002000438A (es) | 2002-07-03 |
Family
ID=7641110
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SV2001000438A SV2002000438A (es) | 2000-05-08 | 2001-05-08 | Purificacion de cuerpos de inclusion de proteinas por microfiltracion de flujo cruzado ref. lea 33419-sv |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US6936699B2 (es) |
| EP (1) | EP1282637B1 (es) |
| JP (1) | JP4818561B2 (es) |
| AR (1) | AR028392A1 (es) |
| AU (1) | AU2001272378A1 (es) |
| CA (1) | CA2408902C (es) |
| DE (2) | DE10022258A1 (es) |
| DO (1) | DOP2001000159A (es) |
| ES (1) | ES2215916T3 (es) |
| MY (1) | MY126838A (es) |
| PE (1) | PE20020003A1 (es) |
| SV (1) | SV2002000438A (es) |
| TW (1) | TWI248940B (es) |
| WO (1) | WO2001085757A2 (es) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10237082B4 (de) * | 2002-08-09 | 2014-12-31 | Sartorius Stedim Biotech Gmbh | Verfahren und Vorrichtung zur biotechnologischen Herstellung von Wertstoffen |
| US20070207461A1 (en) * | 2004-02-23 | 2007-09-06 | Crucell Holland B.V. | Virus Purification Methods |
| CA2602944C (en) * | 2005-04-11 | 2015-08-11 | Crucell Holland B.V. | Virus purification using ultrafiltration |
| EP2174140B1 (en) | 2007-07-03 | 2016-01-13 | Amgen Inc. | Calculation of protein concentration using inclusion body dry weight |
| US8535536B1 (en) | 2009-07-04 | 2013-09-17 | University Of Utah Research Foundation | Cross-flow split-thin-flow cell |
| SG194779A1 (en) | 2011-05-04 | 2013-12-30 | Univ Cornell | Multiblock copolymer films, methods of making same, and uses thereof |
| CA2857307A1 (en) * | 2011-12-01 | 2013-06-06 | Barofold, Inc. | Methods and systems for protein refolding |
| SG11201600380VA (en) * | 2013-07-19 | 2016-02-26 | Biogenomics Ltd | Apparatus for refolding of recombinant proteins |
| JP2015065942A (ja) * | 2013-09-30 | 2015-04-13 | 富士フイルム株式会社 | 細胞培養担体および細胞培養容器 |
| KR101663036B1 (ko) | 2014-07-11 | 2016-10-06 | 한국과학기술원 | 미세조류 농축 및 수소 생성 장치 |
| EP3221687B1 (en) * | 2014-11-17 | 2019-05-01 | Syddansk Universitet | Assembly for assessing drug permeability with adjustable biomimetic properties |
| WO2017189697A1 (en) | 2016-04-28 | 2017-11-02 | Terapore Technologies, Inc. | Charged isoporous materials for electrostatic separations |
| MX2019005774A (es) | 2016-11-17 | 2020-02-07 | Terapore Tech Inc | Películas isoporosas de copolímero de bloques autoensamblados que contienen aditivos hidrofílicos de alto peso molecular y métodos para hacer las mismas. |
| WO2018156731A1 (en) * | 2017-02-22 | 2018-08-30 | Dorin Rachel M | Ligand bound mbp membranes, uses and method of manufacturing |
| CA3062637A1 (en) | 2017-05-12 | 2018-11-15 | Terapore Technologies, Inc. | Chemically resistant fluorinated multiblock polymer structures, methods of manufacturing and use |
| CN111107924B (zh) | 2017-09-19 | 2022-12-27 | 特拉波雷技术有限公司 | 耐化学品的均孔交联嵌段共聚物结构 |
| MX2020009506A (es) | 2018-03-12 | 2021-01-15 | Terapore Tech Inc | Materiales de copolímero de bloques asimétricos mesoporosos isoporosos con macrohuecos y método para fabricarlo. |
| CN112452157B (zh) * | 2020-11-26 | 2023-05-26 | 济南磐升生物技术有限公司 | 一种胶原蛋白提取纯化装置 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4137333A1 (de) | 1991-11-13 | 1993-05-19 | W Prof Dr Sebald | Therapeutische mittel, die antagonisten oder partielle agonisten des humanen interleukin 4 sind oder diese enthalten, hil-4-mutantenproteine sowie vefahren zu deren herstellung |
| US5616595A (en) * | 1995-06-07 | 1997-04-01 | Abbott Laboratories | Process for recovering water insoluble compounds from a fermentation broth |
| EP0853123A1 (de) * | 1997-01-10 | 1998-07-15 | Roche Diagnostics GmbH | Reinigung von DNA durch Cross-Flow-Filtration |
| EP1022337B1 (en) | 1999-01-20 | 2006-07-26 | Bayer HealthCare AG | Plasmids, their construction and their use in the manufacture of interleukin-4 and interleukin-4 muteins |
| DE69929638T2 (de) | 1999-01-20 | 2006-09-21 | Bayer Healthcare Ag | Plasmide, deren Konstruktion und Anwendung zur Herstellung von Interleukin-4 und dessen Muteine |
-
2000
- 2000-05-08 DE DE10022258A patent/DE10022258A1/de not_active Withdrawn
-
2001
- 2001-04-20 TW TW090109473A patent/TWI248940B/zh active
- 2001-04-25 WO PCT/EP2001/004634 patent/WO2001085757A2/de not_active Ceased
- 2001-04-25 US US10/275,797 patent/US6936699B2/en not_active Expired - Fee Related
- 2001-04-25 CA CA2408902A patent/CA2408902C/en not_active Expired - Fee Related
- 2001-04-25 ES ES01951459T patent/ES2215916T3/es not_active Expired - Lifetime
- 2001-04-25 AU AU2001272378A patent/AU2001272378A1/en not_active Abandoned
- 2001-04-25 EP EP01951459A patent/EP1282637B1/de not_active Expired - Lifetime
- 2001-04-25 DE DE50101661T patent/DE50101661D1/de not_active Expired - Lifetime
- 2001-04-25 JP JP2001582356A patent/JP4818561B2/ja not_active Expired - Fee Related
- 2001-04-30 AR ARP010102037A patent/AR028392A1/es active IP Right Grant
- 2001-05-04 MY MYPI20012100 patent/MY126838A/en unknown
- 2001-05-04 DO DO2001000159A patent/DOP2001000159A/es unknown
- 2001-05-08 SV SV2001000438A patent/SV2002000438A/es not_active Application Discontinuation
- 2001-05-08 PE PE2001000410A patent/PE20020003A1/es not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| TWI248940B (en) | 2006-02-11 |
| US20030171560A1 (en) | 2003-09-11 |
| AR028392A1 (es) | 2003-05-07 |
| DE10022258A1 (de) | 2001-11-15 |
| ES2215916T3 (es) | 2004-10-16 |
| MY126838A (en) | 2006-10-31 |
| AU2001272378A1 (en) | 2001-11-20 |
| DE50101661D1 (de) | 2004-04-15 |
| DOP2001000159A (es) | 2002-05-15 |
| JP4818561B2 (ja) | 2011-11-16 |
| PE20020003A1 (es) | 2002-03-28 |
| CA2408902A1 (en) | 2002-11-05 |
| CA2408902C (en) | 2010-09-07 |
| WO2001085757A2 (de) | 2001-11-15 |
| EP1282637B1 (de) | 2004-03-10 |
| EP1282637A2 (de) | 2003-02-12 |
| JP2003535582A (ja) | 2003-12-02 |
| US6936699B2 (en) | 2005-08-30 |
| WO2001085757A3 (de) | 2002-03-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| SV2002000438A (es) | Purificacion de cuerpos de inclusion de proteinas por microfiltracion de flujo cruzado ref. lea 33419-sv | |
| ES2302855T3 (es) | Dispositivo y procedimiento de concentracion y de deteccion de germenes patogenos a partir de productos sanguineos y/o de sus derivados. | |
| ATE327033T1 (de) | Geladene filtrationmembranen und deren verwendungen | |
| ES2119821T3 (es) | Fraccion de calostro y su utilizacion como suplemento en los medios de cultivo celular. | |
| RU2007110534A (ru) | Способ концентрирования препаратов антител | |
| ES541608A0 (es) | Un procedimiento para recuperar interleuquina-2(il-2)a par- tir de un organismo transformado que la contiene | |
| ATE75621T1 (de) | Integrierte vorrichtung zur biospezifischen reinigung einer zellen enthaltenden fluessigkeit. | |
| BR9610445A (pt) | Produção de água de alta pureza usando osmose reversa | |
| RU2002111335A (ru) | Среда для культивирования клеток без белков и без сыворотки | |
| ATE287948T1 (de) | Plasmide dna reinigung durch peg-fällung und säulen-chromatographie | |
| DK0554389T4 (da) | Molekylære kloner af HIV-1 og anvendelse deraf | |
| DK94084A (da) | Fremgangsmaade til fraskillelse af desoxyribonucleinsyre fra ribonucleinsyre i raanucleinsyreoploesninger | |
| MEP56408A (en) | Conjugates and methods for the production thereof, and their use for transporting molecules via biological membranes | |
| DE69928961D1 (de) | Thromboplastin-reagenzien und verfahren zur herstellung und anwendung derselben | |
| BR0016212A (pt) | Proteìna especìfica do cromossoma sexual e sequência de ácido nucleico isolada e purificada, método para identificar moléculas especìficas de cromossomas sexuais associadas com membranas celulares de esperma animal, anticorpo contra um epìtopo de uma molécula especìfica de cromossomas sexuais, métodos de triagem para uma molécula especifica de cromossomas sexuais, e para separar esperma determinante de macho e fêmea de sêmen nativo kit para executar um método, contraceptivo, proteìna especìfica do cromossoma sexual isolada e purificada, e , molécula | |
| YU55891A (sh) | Postupak za izdvajanje vode iz razblaženog vodenog rastvora n-metilformolina-n-oksida, n-metilformolina, morfolina ili njihovih smeša | |
| BRPI0407459A (pt) | Método para recuperar ácido de uma mistura de alimentação, e, membrana polimérica | |
| UY26691A1 (es) | Purificación de cuerpos de inclusión de proteínas por microfiltración de flujo cruzado | |
| BR0002694A (pt) | Processo de obtenção de vacina contra raiva em células vero, para uso humano | |
| ES2154230B1 (es) | Membrana de polimero para la separacion de mezclas de gases. | |
| ES2110515T3 (es) | Purificacion del factor xiii. | |
| RU98112304A (ru) | Способ концентрирования споровых культур в производстве сибиреязвенных вакцинных препаратов | |
| ATE72830T1 (de) | Verfahren zur herstellung von gereinigter glukoseisomerase. | |
| NISHIMURA et al. | Concentration and purification of eel virus, EVE | |
| AU9210401A (en) | Selective binding materials |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FD | Lapse |