PE20200959A1 - Planta de stevia con alto tenor de rebaudiosido m - Google Patents

Planta de stevia con alto tenor de rebaudiosido m

Info

Publication number
PE20200959A1
PE20200959A1 PE2020000428A PE2020000428A PE20200959A1 PE 20200959 A1 PE20200959 A1 PE 20200959A1 PE 2020000428 A PE2020000428 A PE 2020000428A PE 2020000428 A PE2020000428 A PE 2020000428A PE 20200959 A1 PE20200959 A1 PE 20200959A1
Authority
PE
Peru
Prior art keywords
plant
homozygous
seq
position corresponding
base
Prior art date
Application number
PE2020000428A
Other languages
English (en)
Inventor
Kazunari Iwaki
Katsuro Miyagawa
Tadayoshi HIRAI
Naoko Okitsu
Saori TAKEYAMA
Original Assignee
Suntory Holdings Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suntory Holdings Ltd filed Critical Suntory Holdings Ltd
Publication of PE20200959A1 publication Critical patent/PE20200959A1/es

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/14—Asteraceae or Compositae, e.g. safflower, sunflower, artichoke or lettuce
    • A01H6/1488—Stevia
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/06—Roots
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/52—Adding ingredients
    • A23L2/60—Sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/46—Ingredients of undetermined constitution or reaction products thereof, e.g. skin, bone, milk, cotton fibre, eggshell, oxgall or plant extracts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Physiology (AREA)
  • Environmental Sciences (AREA)
  • Biomedical Technology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Molecular Biology (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Medical Informatics (AREA)
  • Plant Pathology (AREA)

Abstract

Se refiere a una planta de Stevia con elevado tenor de rebaudiosido M y no modificada en forma genetica, que consta de 2 %, o mas, de rebaudiosido M respecto de la cantidad de glicosido total de esteviol que contiene en una hoja. La planta tiene como minimo uno de los rasgos geneticos siguientes: (1) Homocigota para el alelo que se caracteriza porque la base en la posicion correspondiente a la posicion 44 de la SEQ ID NO: 35 es T. (2) Homocigota para el alelo que se caracteriza porque la base en la posicion correspondiente a la posicion 40 de la SEQ ID NO: 37 es T. (3) Homocigota para el alelo que se caracteriza porque la base en la posicion correspondiente a la posicion 41 de la SEQ ID NO: 39 es C. (4) Homocigota para el alelo que se caracteriza porque el segmento en la posicion correspondiente a las posiciones 55-72 de la SEQ ID NO: 42 esta suprimida. (5) Homocigota para el alelo que se caracteriza porque la base en la posicion correspondiente a la posicion 50 de la SEQ ID NO: 43 es A. La planta se caracteriza porque es positiva para, como minimo, un marcador polimorfico que se selecciona del grupo consistente en P01 a P05. Tambien se refiere a un metodo para la produccion de dicha planta, un metodo para fabricar un extracto que contiene rebaudiosido M y a un metodo para hacer el cribado de dicha planta.
PE2020000428A 2017-10-12 2018-10-12 Planta de stevia con alto tenor de rebaudiosido m PE20200959A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2017198515 2017-10-12
PCT/JP2018/038064 WO2019074089A1 (ja) 2017-10-12 2018-10-12 高レバウジオシドｍ含有ステビア植物

Publications (1)

Publication Number Publication Date
PE20200959A1 true PE20200959A1 (es) 2020-09-24

Family

ID=66101618

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2020000428A PE20200959A1 (es) 2017-10-12 2018-10-12 Planta de stevia con alto tenor de rebaudiosido m

Country Status (10)

Country Link
US (1) US12317798B2 (es)
EP (1) EP3695714A4 (es)
JP (2) JP7670459B2 (es)
CN (1) CN111163631A (es)
AR (1) AR113768A1 (es)
AU (1) AU2018350050A1 (es)
BR (1) BR112020006485A2 (es)
PE (1) PE20200959A1 (es)
PY (1) PY1889766A (es)
WO (1) WO2019074089A1 (es)

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Publication number Priority date Publication date Assignee Title
WO2020027155A1 (ja) * 2018-07-31 2020-02-06 サントリーホールディングス株式会社 甘味成分高含有型ステビア植物及びそのスクリーニング方法
AR117736A1 (es) * 2018-12-28 2021-08-25 Suntory Holdings Ltd Planta de stevia con alto contenido de rebaudiósido d
MY209464A (en) 2019-04-11 2025-07-10 Suntory Holdings Ltd Stevia plant having less ability to form pollens
WO2020209265A1 (ja) * 2019-04-11 2020-10-15 サントリーホールディングス株式会社 花芽形成能の低いステビア植物
AU2020323449A1 (en) * 2019-07-31 2022-03-03 Suntory Holdings Limited Plant body containing novel steviol glycoside
EP4033888A4 (en) * 2019-09-24 2023-12-13 PureCircle USA Inc. STEVIA CULTIVAR '320032' WITH SUPER HIGH REBAUDIOSIDE CONTENT
JP7561751B2 (ja) 2019-11-01 2024-10-04 サントリーホールディングス株式会社 レバウディオサイドｄ含有晶析物の製造方法およびレバウディオサイドｄ含有晶析物
PY2070811A (es) * 2019-11-01 2021-08-12 Purecircle Usa Inc Cultivar de stevia 18136109
JP7187710B2 (ja) 2019-11-01 2022-12-12 サントリーホールディングス株式会社 レバウディオサイドｄ含有晶析物の製造方法およびレバウディオサイドｄ含有晶析物
PY2137784A (es) * 2020-05-12 2022-01-27 Suntory Holdings Ltd Planta de stevia con un contenido alto de glucósidos de esteviol y método para su selección
EP4166153A4 (en) 2020-05-12 2024-05-29 Suntory Holdings Limited Rebaudioside d-rich stevia plant
JPWO2021230257A1 (es) * 2020-05-12 2021-11-18
CN115551347A (zh) * 2020-05-12 2022-12-30 三得利控股株式会社 富含瑞鲍迪苷e的甜菊植物体
WO2022220152A1 (ja) * 2021-04-15 2022-10-20 サントリーホールディングス株式会社 栄養成分リッチなステビア植物
CN113229136B (zh) * 2021-05-25 2022-02-11 南京农业大学 一种甜叶菊叶片品质育种亲本选配方法
AR129756A1 (es) 2022-06-30 2024-09-25 Suntory Holdings Ltd Método de selección de una planta de stevia
AR134506A1 (es) * 2023-12-01 2026-01-21 Suntory Holdings Ltd Planta de stevia con alto contenido de rebaudiósido m

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US5925525A (en) 1989-06-07 1999-07-20 Affymetrix, Inc. Method of identifying nucleotide differences
US5858659A (en) 1995-11-29 1999-01-12 Affymetrix, Inc. Polymorphism detection
US6045996A (en) 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US8377491B2 (en) 2005-11-23 2013-02-19 The Coca-Cola Company High-potency sweetener composition with vitamin and compositions sweetened therewith
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PE20200961A1 (es) 2013-03-15 2020-09-24 Cargill Inc Plantas de stevia con un contenido de rebaudiosido d incrementado
CN104151378A (zh) 2014-08-12 2014-11-19 济南汉定生物工程有限公司 一种甜菊糖甙rm的提纯方法
US10370673B2 (en) 2014-09-26 2019-08-06 Purecircle Sdn Bhd Single nucleotide polymorphism (SNP) markers for stevia
CN109006460B (zh) 2015-01-23 2022-03-22 谱赛科美国公司 甜叶菊新品种817096谱星5号及高rd含量甜菊糖苷的制备
WO2017171023A1 (ja) 2016-03-31 2017-10-05 サントリーホールディングス株式会社 ヘスペリジンの沈殿が抑制された飲料
JP6741913B2 (ja) 2016-04-27 2020-08-19 パナソニックＩｐマネジメント株式会社 磁気センサこれを用いた回転検出装置

Also Published As

Publication number Publication date
US20200281141A1 (en) 2020-09-10
EP3695714A1 (en) 2020-08-19
US12317798B2 (en) 2025-06-03
BR112020006485A2 (pt) 2020-10-13
CN111163631A (zh) 2020-05-15
PY1889766A (es) 2019-05-01
JP7670459B2 (ja) 2025-04-30
AU2018350050A1 (en) 2020-04-02
JP7756123B2 (ja) 2025-10-17
EP3695714A4 (en) 2021-07-21
AR113768A1 (es) 2020-06-10
WO2019074089A1 (ja) 2019-04-18
JPWO2019074089A1 (ja) 2020-11-26
JP2023075257A (ja) 2023-05-30

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