PH30485A - Secretion of hirudin derivatives - Google Patents

Secretion of hirudin derivatives

Info

Publication number: PH30485A
Authority: PH; Philippines
Prior art keywords: hirudin; recombinant vector; hirudin derivative; tyr; thr
Prior art date: 1990-03-22

Application number

PH42047A

Other languages

English (en)

Inventor

Gerhard Dr Schmid

Paul Dr Haberman

Original Assignee

Consortium Fur Electrachemisch

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-03-22

Filing date

1991-02-22

Publication date

1997-05-28

1991-02-22 Application filed by Consortium Fur Electrachemisch filed Critical Consortium Fur Electrachemisch

1997-05-28 Publication of PH30485A publication Critical patent/PH30485A/en

Links

WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical class C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 title abstract 6
230000028327 secretion Effects 0.000 title 1
108090000623 proteins and genes Proteins 0.000 abstract 3
241000588724 Escherichia coli Species 0.000 abstract 2
108010076504 Protein Sorting Signals Proteins 0.000 abstract 2
230000001580 bacterial effect Effects 0.000 abstract 2
125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 abstract 1
102000004169 proteins and genes Human genes 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C12N9/1074—Cyclomaltodextrin glucanotransferase (2.4.1.19)
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
General Health & Medical Sciences (AREA)
Molecular Biology (AREA)
Zoology (AREA)
Medicinal Chemistry (AREA)
Biomedical Technology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Biophysics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Gastroenterology & Hepatology (AREA)
Tropical Medicine & Parasitology (AREA)
Animal Behavior & Ethology (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Pharmacology & Pharmacy (AREA)
Diabetes (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Hematology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Indole Compounds (AREA)
Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

PH42047A 1990-03-22 1991-02-22 Secretion of hirudin derivatives PH30485A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE4009268A DE4009268A1 (de)	1990-03-22	1990-03-22	Sekretion von hirudinderivaten

Publications (1)

Publication Number	Publication Date
PH30485A true PH30485A (en)	1997-05-28

Family

ID=6402843

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
PH42047A PH30485A (en)	1990-03-22	1991-02-22	Secretion of hirudin derivatives

Country Status (23)

Country	Link
US (1)	US5919895A (fr)
EP (1)	EP0448093B1 (fr)
JP (1)	JPH07106157B2 (fr)
KR (1)	KR950007510B1 (fr)
CN (1)	CN1067104C (fr)
AT (1)	ATE135042T1 (fr)
AU (1)	AU640212B2 (fr)
BR (1)	BR9101104A (fr)
CA (1)	CA2038888C (fr)
DE (2)	DE4009268A1 (fr)
DK (1)	DK0448093T3 (fr)
ES (1)	ES2084052T3 (fr)
FI (1)	FI100250B (fr)
GR (1)	GR3019946T3 (fr)
HU (1)	HU214368B (fr)
IE (1)	IE71174B1 (fr)
IL (1)	IL97323A (fr)
NO (1)	NO308543B1 (fr)
NZ (2)	NZ245885A (fr)
PH (1)	PH30485A (fr)
PT (1)	PT97093B (fr)
RU (1)	RU2118365C1 (fr)
ZA (1)	ZA912013B (fr)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3835815A1 (de) *	1988-10-21	1990-04-26	Hoechst Ag	Neue isohirudine
DE4140381A1 (de) *	1991-12-07	1993-06-09	Hoechst Ag, 6230 Frankfurt, De	Neue synthetische isohirudine mit verbesserter stabilitaet
SE9203753D0 (sv) *	1992-12-11	1992-12-11	Kabi Pharmacia Ab	Expression system for producing apolipoprotein ai-m
AU3673495A (en)	1995-04-12	1996-10-30	Kyowa Hakko Kogyo Co. Ltd.	Plant cell growth factor
JP3746847B2 (ja) *	1996-08-02	2006-02-15	協和醗酵工業株式会社	植物細胞増殖因子
DE19915938A1 (de)	1999-04-09	2000-10-19	Aventis Pharma Gmbh	Herstellung von pankreatischer Procarboxypeptidase B, Isoformen und Muteinen davon und ihre Verwendung
DE19944870A1 (de)	1999-09-18	2001-03-29	Aventis Pharma Gmbh	Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium
DE10033195A1 (de) *	2000-07-07	2002-03-21	Aventis Pharma Gmbh	Bifunktionale Fusionsproteine aus Hirudin und TAP
US7202059B2 (en)	2001-02-20	2007-04-10	Sanofi-Aventis Deutschland Gmbh	Fusion proteins capable of being secreted into a fermentation medium
US7638618B2 (en)	2001-02-20	2009-12-29	Sanofi-Aventis Deutschland Gmbh	Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
DE10108211A1 (de) *	2001-02-20	2002-08-22	Aventis Pharma Gmbh	Verwendung von Fusionsproteinen, deren N-terminaler Anteil aus einem Hirudinderivat besteht, zur Herstellung rekombinanter Proteine über Sekretion durch Hefen
DE102006004871A1 (de)	2006-02-02	2007-08-09	Wacker Chemie Ag	Mikroorganismenstamm zur Produktion von rekombinanten Proteinen
DK1905839T4 (da) *	2006-09-22	2019-10-07	Wacker Chemie Ag	Fremgangsmåde til fermentativ fremstilling af proteiner
EP1903105B1 (fr) *	2006-09-22	2010-04-21	Wacker Chemie AG	Procédé pour la production de protéines par fermentation
DK1903115T3 (da)	2006-09-22	2011-05-23	Wacker Chemie Ag	Fremgangsmåde til fermentativ fremstilling af antistoffer
DE102006044841A1 (de)	2006-09-22	2008-04-03	Wacker Chemie Ag	Signalpeptid zur Produktion von rekombinanten Proteinen
DE102006050332A1 (de) *	2006-10-25	2008-04-30	Wacker Chemie Ag	DNS-Konstrukt und Verfahren zur fermentativen Herstellung von Fusionsproteinen
DE102008063900A1 (de)	2008-12-19	2010-06-24	Wacker Chemie Ag	Verfahren zur fermentativen Herstellung von heterologen Proteinen mittels Escherichia coli
CN103848914B (zh) *	2012-11-29	2018-08-28	河北以岭医药研究院有限公司	一种具抗凝活性的菲牛蛭素多肽及其制备方法与用途
CN103059130A (zh) *	2012-12-22	2013-04-24	浙江工业大学	一种水蛭素突变体及重组基因工程菌
CN108220369B (zh) *	2017-12-04	2021-03-12	广东双骏生物科技有限公司	一种生产重组水蛭素的方法
KR102546461B1 (ko)	2018-07-24	2023-06-21	와커 헤미 아게	신규한 박테리아 lpp 돌연변이체 및 재조합 단백질의 분비 생산을 위한 그의 용도
CN115572329B (zh) *	2021-06-21	2024-02-06	王大勇	一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE2936543A1 (de) *	1979-09-10	1981-04-09	Behringwerke Ag, 3550 Marburg	Chromogene verbindungen
AU561343B2 (en) *	1981-10-19	1987-05-07	Genentech Inc.	Human immune interferon by recombinant dna
WO1985004418A1 (fr) *	1984-03-27	1985-10-10	Transgene S.A.	Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
DE3438296A1 (de) *	1984-04-18	1985-11-07	Hoechst Ag, 6230 Frankfurt	Neue polypeptide mit blutgerinnungshemmender wirkung, verfahren zu deren herstellung bzw. gewinnung, deren verwendung und diese enthaltende mittel
GB8412517D0 (en) *	1984-05-16	1984-06-20	Nagai K	Recombinant fusion proteins
ATE64956T1 (de) *	1984-06-14	1991-07-15	Ciba Geigy Ag	Verfahren zur herstellung von thrombininhibitoren.
DE3429430A1 (de) *	1984-08-10	1986-02-20	Hoechst Ag, 6230 Frankfurt	Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
DE3445517C2 (de) *	1984-12-13	1993-11-18	Ciba Geigy	Für ein Hirudin-ähnliches Protein codierende DNA-Sequenz und Verfahren zur Herstellung eines Hirudin-ähnlichen Proteins
US4923808A (en) *	1985-03-12	1990-05-08	Genentech, Inc.	Method for identifying mutants secreting high levels of heterologous proteins
ZA89215B (en) *	1985-04-11	1993-12-23	Hoechst Ag	A hirundi derivative
FR2593518B1 (fr) *	1985-05-02	1989-09-08	Transgene Sa	Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
MY101203A (en) *	1985-12-12	1991-08-17	Ucp Gen Pharma Ag	Production of thrombin iinhibitors.
MC1834A1 (fr) *	1986-07-10	1988-06-03	Transgene Sa	Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation
AU604925B2 (en) *	1988-02-23	1991-01-03	Schering Aktiengesellschaft	A hirudin derivative
DE3813107A1 (de) *	1988-04-19	1989-11-02	Consortium Elektrochem Ind	Sekretormutante von escherichia coli
GB8817160D0 (en) *	1988-07-19	1988-08-24	Ciba Geigy Ag	Novel proteins
JPH0648988B2 (ja) *	1988-07-26	1994-06-29	工業技術院長	トロンビン阻害物質の製造法
FR2636643B1 (fr) *	1988-08-24	1990-12-28	Sanofi Sa	Peptide-signal, sequences d'adn codant pour celui-ci, vecteurs d'expression portant l'une de ces sequences et procede de production periplasmique d'un polypeptide
GB8826428D0 (en) *	1988-11-11	1988-12-14	Biopharm Ltd	Antithrombin
US5164304A (en) *	1989-05-04	1992-11-17	Sri International	Method and vectors for stabilizing hirudin and human laminin b1 expression
ES2129749T3 (es) *	1990-11-08	1999-06-16	Japan Energy Corp	Vector de secrecion, microorganismos transformados que contienen el indicado vector y produccion de un producto a partir de dicho microorganismo.

1990
- 1990-03-22 DE DE4009268A patent/DE4009268A1/de not_active Withdrawn
1991
- 1991-02-21 IL IL9732391A patent/IL97323A/xx not_active IP Right Cessation
- 1991-02-22 PH PH42047A patent/PH30485A/en unknown
- 1991-02-28 FI FI911009A patent/FI100250B/fi not_active IP Right Cessation
- 1991-03-05 IE IE73891A patent/IE71174B1/en not_active IP Right Cessation
- 1991-03-14 NZ NZ245885A patent/NZ245885A/xx unknown
- 1991-03-14 NZ NZ237437A patent/NZ237437A/xx unknown
- 1991-03-18 KR KR1019910004246A patent/KR950007510B1/ko not_active Expired - Fee Related
- 1991-03-19 ZA ZA912013A patent/ZA912013B/xx unknown
- 1991-03-20 JP JP3056714A patent/JPH07106157B2/ja not_active Expired - Fee Related
- 1991-03-21 DK DK91104422.0T patent/DK0448093T3/da active
- 1991-03-21 PT PT97093A patent/PT97093B/pt not_active IP Right Cessation
- 1991-03-21 DE DE59107495T patent/DE59107495D1/de not_active Expired - Fee Related
- 1991-03-21 HU HU91953A patent/HU214368B/hu not_active IP Right Cessation
- 1991-03-21 AU AU73680/91A patent/AU640212B2/en not_active Ceased
- 1991-03-21 ES ES91104422T patent/ES2084052T3/es not_active Expired - Lifetime
- 1991-03-21 EP EP91104422A patent/EP0448093B1/fr not_active Expired - Lifetime
- 1991-03-21 BR BR919101104A patent/BR9101104A/pt not_active Application Discontinuation
- 1991-03-21 AT AT91104422T patent/ATE135042T1/de not_active IP Right Cessation
- 1991-03-21 CN CN91101687A patent/CN1067104C/zh not_active Expired - Fee Related
- 1991-03-21 RU SU4894968A patent/RU2118365C1/ru not_active IP Right Cessation
- 1991-03-21 NO NO911137A patent/NO308543B1/no unknown
- 1991-03-22 CA CA002038888A patent/CA2038888C/fr not_active Expired - Fee Related
1992
- 1992-11-25 US US07/982,064 patent/US5919895A/en not_active Expired - Fee Related
1996
- 1996-05-17 GR GR960401313T patent/GR3019946T3/el unknown

Also Published As

Publication number	Publication date
HU214368B (hu)	1998-03-30
KR920018217A (ko)	1992-10-21
BR9101104A (pt)	1991-11-05
IE71174B1 (en)	1997-01-29
IL97323A0 (en)	1992-05-25
NO911137L (no)	1991-09-23
DK0448093T3 (da)	1996-04-09
HU910953D0 (en)	1991-10-28
ES2084052T3 (es)	1996-05-01
RU2118365C1 (ru)	1998-08-27
NZ245885A (en)	1993-11-25
IE910738A1 (en)	1991-09-25
CN1055010A (zh)	1991-10-02
FI911009A0 (fi)	1991-02-28
GR3019946T3 (en)	1996-08-31
AU7368091A (en)	1991-10-03
KR950007510B1 (ko)	1995-07-11
NO308543B1 (no)	2000-09-25
CN1067104C (zh)	2001-06-13
EP0448093A2 (fr)	1991-09-25
EP0448093B1 (fr)	1996-03-06
CA2038888A1 (fr)	1991-09-23
EP0448093A3 (en)	1991-12-04
DE4009268A1 (de)	1991-09-26
CA2038888C (fr)	1998-12-29
JPH04211391A (ja)	1992-08-03
NZ237437A (en)	1993-11-25
IL97323A (en)	1997-01-10
NO911137D0 (no)	1991-03-21
JPH07106157B2 (ja)	1995-11-15
FI911009L (fi)	1991-09-23
PT97093B (pt)	1998-08-31
AU640212B2 (en)	1993-08-19
PT97093A (pt)	1991-11-29
DE59107495D1 (de)	1996-04-11
HUT59962A (en)	1992-07-28
US5919895A (en)	1999-07-06
ATE135042T1 (de)	1996-03-15
FI100250B (fi)	1997-10-31
ZA912013B (en)	1991-12-24

Publication	Publication Date	Title
EP0448093A3 (en)	1991-12-04	Secretion of hirudin derivatives
WO1994012638A3 (fr)	1994-09-01	Polypeptides a mutations multiples d'interleukine-3
NL300013I2 (nl)	2001-04-02	Nieuwe proteinen me TNF-remmende werking en hun bereiding.
AU5470194A (en)	1994-03-24	Hirudin mutant, production thereof, anticoagulant, secretory vector, and production of product from said microorganism
NO942553D0 (no)	1994-07-06	Ny humanproteaseinhibitor av Kunitz-type og varianter derav
AU7504096A (en)	1997-06-11	Pseudomonas exotoxin - myelin basic protein chimeric proteins
EP0528955A4 (en)	1993-06-16	Protease deficient bacterial hosts
YU200091A (sh)	1994-06-10	Delecioni mutanti ahasarezistentni na herbicide
DE68914397D1 (de)	1994-05-11	Menschliches Lymphotoxin.
Tonoike et al.	1985	Amino acid sequence of an amyloidogenic Bence Jones protein in myeloma‐associated systemic amyloidosis
Nhieu et al.	1987	Primary structure of an aminoglycoside 6'-N-acetyltransferase, AAC (6')-4, fused in vivo with the signal peptide of the Tn 3-encoded beta-lactamase.