PL136563B2 - Method of obtaining new 2-methyl-3-beta-hydroxy-gamma-morpholinopropylo-3,4-dihydropyrido-/2,3-d/-pyrimidin-4-none - Google Patents

Method of obtaining new 2-methyl-3-beta-hydroxy-gamma-morpholinopropylo-3,4-dihydropyrido-/2,3-d/-pyrimidin-4-none Download PDF

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Publication number
PL136563B2
PL136563B2 PL24580684A PL24580684A PL136563B2 PL 136563 B2 PL136563 B2 PL 136563B2 PL 24580684 A PL24580684 A PL 24580684A PL 24580684 A PL24580684 A PL 24580684A PL 136563 B2 PL136563 B2 PL 136563B2
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PL
Poland
Prior art keywords
hydroxy
methyl
pyrimidin
morpholinopropylo
dihydropyrido
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PL24580684A
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Polish (pl)
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PL245806A2 (en
Inventor
Leonard Kuczynski
Jadwiga Soloducho
Aleksander Mrozikiewicz
Teresa Bobkiewiczkozlowska
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Akad Wroclawiu Med
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Priority to PL24580684A priority Critical patent/PL136563B2/en
Publication of PL245806A2 publication Critical patent/PL245806A2/en
Publication of PL136563B2 publication Critical patent/PL136563B2/en

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  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Description

Przedmiotem wynalazku jest sposób wytwarzania nowego 2-metylo-3-/?-hydroksy-y- morfolinopropylo-3,4-dihydropirydo- [2,3-d]-pirymidyn-4-onu o wzorze 1, charakteryzujacego sie dzialaniem hipotensyjnym.Ze stanu techniki nie sa znane sposoby wytwarzania /?-hydroksy-y-morfolinopropylowej pochodnej pirydo-[2,3-d]-pirymidyny.Wedlug wynalazku sposób wytwarzania nowego 2-metylo-3-/?-hydroksy-y- morfolinopropy- lo-3,4-dihydropirydo-[2,3-d]-pirymidyn-4-onu o wzorze 1 polega na tym, ze na 2-aminonikotyno- amid dziala sie nadmiarem acetamidu w temperaturze 403-413 K i otrzymuje 2-metylo-3,4-dihy- dropirydo-[2,3-d]-pirymidyn-4-on o wzorze 2, na który nastepnie dziala sie nadmiarem chlorku j8-hydroksy-y-morfolinopropylowego w temperaturze 363 K.Na podstawie stanu techniki nie mozna bylo z góry przewidziec, ze uzyska sie reakcje przebiegajaca pomiedzy reagentami uzytymi w sposobie wedlug wynalazku i ze otrzyma sie produkt o dzialaniu farmakologicznym. Zwiazek wytworzony sposobem wedlug wynalazku wyka¬ zuje w badaniach farmakologicznych dzialanie hipotensyjne. Zwiazek ten podawany szczurom w dawce 30 mg/kg powoduje spadek cisnienia, który narasta z uplywem minut, osiagajac po 60 minutach od podania 50 mm Hg. W zakresie wplywu zwiazku na czynnosc oddechowa u szczurów, powoduje niewielkie zwolnienie czestosci oddechu. Zwiazek ten w sposób statystycznie znamienny w dawce 50 mg/kg znosi wzrost obwodowego oporu naczyniowego, wywolanego podaniem 50 mcg noradrenaliny oraz zapobiega temu wzrostowi. Toksycznosc przyblizona zwiazku oznaczona w tescie po podaniu dootrzewnowym u myszy wynosi 400 mg/kg.Przedmiot wynalazku jest przedstawiony w przykladzie wykonania.Przyklad. Mieszanine 10 g (0,072 mola) 2-aminonikotynoamidu ogrzewa sie z 5 g(0,08 mola) acetamidu w temperaturze 403-413-K w czasie 4 godzin, a nastepnie ochladza, odfiltrowuje osad i przemywa acetonem, po czym przekrystalizowuje z metanolu. Otrzymuje sie 10,57 g 2-metylo-3,4- -dihydropirydo-[2,3-d]-pirymidyn-4-onu. Do 20 cm3 suchego ksylenu i 0,3g (0,007 mola)amidku sodowego wprowadza sie zawiesine 1 g (0,0062 mola) 2-metylo-3,4-dihydropirydo-[2,3-d]-pirymi-2 136 563 dyn-4-onu w 10cm3 suchego ksylenu. Mieszanine reagentów ogrzewa sie w lekkim wrzeniu 2 godziny, sól sodowa odsacza sie, rozpuszcza sie ja w 20cm3 metanolu, a nastepnie ogrzewa 4 godziny z 1,3 g (0,007 mola) chlorku /^hydroksy-7-morfolinopropylowego. Odsacza sie od nie- przereagowanej soli sodowej, rozpuszczalnik oddestylowuje sie, z pozostalosci wyodrebnia sie krystaliczny produkt, który przekrystalizowuje sie z metanolu. Otrzymuje sie l,56g, co stanowi 83% wydajnosci teoretycznej, 2-metylo-3-j8-hydroksy-7-morlblinopropylo-3,4-dihydropirydo- [2,3-d]-pirymidyn-4-onu. Zwiazek ten jest bezbarwna, krystaliczna substancja o temperaturze topnienia 492-493 K, rozpuszczalna w metanolu, acetonie, dimetylosulfotlenku, tetrahydrofura- nie,'zas nierozpuszczalna w benzenie, wodzie, eterze etylowym i naftowym, która nie podlega zmianom pod wplywem powietrza i swiatla.Zastrzezenie patentowe Sposób wytwarzania nowego 2-metylo-3-j8-hydroksy-7-morfolinopropylo-3,4-dihydropiry- do-[2,3-d]-pirymidyn-4-onu o wzorze 1, znamienny tym, ze na 2-aminonikotynoamid dziala sie nad¬ miarem acetamidu w temperaturze 403-413 K i otrzymuje 2-metylo-3,4-dihydropirydo-[2,3-d]- -pirymidyn-4-on o wzorze 2, na który nastepnie dziala sie nadmiarem chlorku /J-hydroksy-7-mor- folinopropylowego w temperaturze 363 K.Nj-CH,- CH-CHi-l/ ^ CHs OH WZbR 1 WZÓR 2 Pracownia Poligraficzna UP PRL, Naklad 100 egz.Cena 100 zl PLThe subject of the invention is a process for the preparation of the new 2-methyl-3 - / β-hydroxy-y-morpholinopropyl-3,4-dihydropyrid- [2,3-d] -pyrimidin-4-one of the formula 1, which is characterized by a hypotensive effect. There are no known processes for the preparation of the i-β-hydroxy-γ-morpholinopropyl derivative of pyrido [2,3-d] -pyrimidine according to the invention. The 3,4-dihydropyrid- [2,3-d] -pyrimidin-4-one of the formula 1 is based on the treatment of the 2-aminonicotinamide with an excess of acetamide at 403-413 K and gives 2-methyl-3 , 4-dihydropyrid- [2,3-d] -pyrimidin-4-one of the formula 2, which is then treated with an excess of β-hydroxy-γ-morpholinopropyl chloride at a temperature of 363 K. Based on the prior art, it was not possible to predict in advance that a reaction will be obtained between the reactants used in the process according to the invention and that a pharmacological product will be obtained. The compound according to the invention shows a hypotensive effect in pharmacological tests. This compound, administered to rats at a dose of 30 mg / kg, causes a drop in blood pressure that increases with the passage of minutes, reaching 50 mm Hg 60 minutes after administration. In terms of the effect of compound on respiratory function in rats, it causes a slight decrease in the respiratory rate. This compound is statistically significant at the dose of 50 mg / kg and prevents the increase in peripheral vascular resistance caused by the administration of 50 mcg of noradrenaline. The approximate toxicity of the compound determined in the test after intraperitoneal administration in mice is 400 mg / kg. The subject of the invention is illustrated in the embodiment. Example. A mixture of 10 g (0.072 mol) of 2-aminonicotinamide is heated with 5 g (0.08 mol) of acetamide at 403-413-K for 4 hours, then cooled, filtered and washed with acetone, then recrystallized from methanol. 10.57 g of 2-methyl-3,4-dihydropyrid- [2,3-d] -pyrimidin-4-one are obtained. A suspension of 1 g (0.0062 mol) of 2-methyl-3,4-dihydropyrid- [2,3-d] -pyrim-2 136 563 dynes is introduced into 20 cm 3 of dry xylene and 0.3 g (0.007 mol) of sodium amide. -4-one in 10cm3 dry xylene. The mixture of reagents is heated under slight boiling for 2 hours, the sodium salt is filtered off, dissolved in 20 ml of methanol and then heated for 4 hours with 1.3 g (0.007 mol) of N-hydroxy-7-morpholinopropyl chloride. It is filtered off from the unreacted sodium salt, the solvent is distilled off, and a crystalline product is isolated from the residue, which is recrystallized from methanol. 1.56 g (83% of theory) of 2-methyl-3-8-hydroxy-7-morlbinopropyl-3,4-dihydropyrid- [2,3-d] pyrimidin-4-one are obtained. This compound is a colorless, crystalline substance with a melting point of 492-493 K, soluble in methanol, acetone, dimethylsulfoxide, tetrahydrofuran, and insoluble in benzene, water, ethyl and petroleum ether, which is not affected by air and light. Claimed A method for the preparation of a new 2-methyl-3-8-hydroxy-7-morpholinopropyl-3,4-dihydropyrino- [2,3-d] -pyrimidin-4-one of formula 1, characterized by The -aminonicotinamide is treated with an excess of acetamide at 403-413 K to give 2-methyl-3,4-dihydropyrid- [2,3-d] -pyrimidin-4-one of the formula II, which is then treated with excess chloride / J-hydroxy-7-morpholinopropyl at the temperature of 363 K.Nj-CH, - CH-CHi-l / ^ CHs OH WZbR 1 MODEL 2 Printing workshop of the Polish People's Republic, Circulation 100 copies Price PLN 100 PL

Claims (4)

Zastrzezenie patentowe 1. Sposób wytwarzania nowego 2-metylo-3-j8-hydroksy-7-morfolinopropylo-3,4-dihydropiry- do-[2,3-d]-pirymidyn-4-onu o wzorze 1, znamienny tym, ze na 2-aminonikotynoamid dziala sie nad¬ miarem acetamidu w temperaturze 403-413 K i otrzymujeClaim 1. A process for the preparation of a new 2-methyl-3-j8-hydroxy-7-morpholinopropyl-3,4-dihydropyrino- [2,3-d] -pyrimidin-4-one of the formula 1, characterized by the 2-aminonicotinamide is treated with an excess of acetamide at a temperature of 403-413 K and gives 2. -metylo-3,4-dihydropirydo-[2,2.-methyl-3,4-dihydropyrid- [2, 3. -d]- -pirymidyn-3. -d] - -pyrimidine- 4. -on o wzorze 2, na który nastepnie dziala sie nadmiarem chlorku /J-hydroksy-7-mor- folinopropylowego w temperaturze 363 K. Nj-CH,- CH-CHi-l/ ^ CHs OH WZbR 1 WZÓR 2 Pracownia Poligraficzna UP PRL, Naklad 100 egz. Cena 100 zl PL4. -one of formula 2, which is then treated with an excess of / J-hydroxy-7-morpholinopropyl chloride at a temperature of 363 K. Nj-CH, - CH-CHi-1 / ^ CHs OH WZbR 1 MODEL 2 Printing workshop UP PRL, Circulation 100 copies. Price PLN 100 PL
PL24580684A 1984-01-19 1984-01-19 Method of obtaining new 2-methyl-3-beta-hydroxy-gamma-morpholinopropylo-3,4-dihydropyrido-/2,3-d/-pyrimidin-4-none PL136563B2 (en)

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PL24580684A PL136563B2 (en) 1984-01-19 1984-01-19 Method of obtaining new 2-methyl-3-beta-hydroxy-gamma-morpholinopropylo-3,4-dihydropyrido-/2,3-d/-pyrimidin-4-none

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PL24580684A PL136563B2 (en) 1984-01-19 1984-01-19 Method of obtaining new 2-methyl-3-beta-hydroxy-gamma-morpholinopropylo-3,4-dihydropyrido-/2,3-d/-pyrimidin-4-none

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PL136563B2 true PL136563B2 (en) 1986-02-28

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