PL2697376T3 - Sposób ekspresji z pomocą proteazy pomocniczej - Google Patents
Sposób ekspresji z pomocą proteazy pomocniczejInfo
- Publication number
- PL2697376T3 PL2697376T3 PL12713714T PL12713714T PL2697376T3 PL 2697376 T3 PL2697376 T3 PL 2697376T3 PL 12713714 T PL12713714 T PL 12713714T PL 12713714 T PL12713714 T PL 12713714T PL 2697376 T3 PL2697376 T3 PL 2697376T3
- Authority
- PL
- Poland
- Prior art keywords
- helper
- protease
- expression method
- expression
- helper protease
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102011007313A DE102011007313A1 (de) | 2011-04-13 | 2011-04-13 | Expressionsverfahren |
| EP12713714.9A EP2697376B1 (de) | 2011-04-13 | 2012-04-05 | Expressionsverfahren mit einer hilfsprotease |
| PCT/EP2012/056262 WO2012139964A1 (de) | 2011-04-13 | 2012-04-05 | Expressionsverfahren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PL2697376T3 true PL2697376T3 (pl) | 2017-10-31 |
Family
ID=45952535
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL12713714T PL2697376T3 (pl) | 2011-04-13 | 2012-04-05 | Sposób ekspresji z pomocą proteazy pomocniczej |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US9663798B2 (pl) |
| EP (1) | EP2697376B1 (pl) |
| JP (2) | JP6099627B2 (pl) |
| KR (1) | KR20140019436A (pl) |
| CN (1) | CN103608457A (pl) |
| BR (1) | BR112013025951A2 (pl) |
| CA (1) | CA2831473A1 (pl) |
| DE (1) | DE102011007313A1 (pl) |
| DK (1) | DK2697376T3 (pl) |
| ES (1) | ES2639114T3 (pl) |
| MX (1) | MX348766B (pl) |
| PL (1) | PL2697376T3 (pl) |
| RU (1) | RU2642324C2 (pl) |
| WO (1) | WO2012139964A1 (pl) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102011118032A1 (de) | 2011-05-31 | 2012-12-06 | Henkel Ag & Co. Kgaa | Expressionsvektoren zur verbesserten Proteinsekretion |
| CN104059869B (zh) * | 2014-06-20 | 2016-07-06 | 淮海工学院 | 一株海洋解淀粉芽孢杆菌及其培养方法和应用 |
| EP3717643A1 (en) | 2017-11-29 | 2020-10-07 | Danisco US Inc. | Subtilisin variants having improved stability |
| KR102112240B1 (ko) * | 2018-09-28 | 2020-05-18 | 씨제이제일제당 주식회사 | 알파-글루코시다제의 활성이 강화된 l-아미노산을 생산하는 미생물 및 이를 이용한 l-아미노산 생산 방법 |
| US12509672B2 (en) | 2018-11-28 | 2025-12-30 | Danisco Us Inc. | Subtilisin variants having improved stability |
Family Cites Families (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK6488D0 (da) * | 1988-01-07 | 1988-01-07 | Novo Industri As | Enzymer |
| ATE187490T1 (de) | 1989-08-25 | 1999-12-15 | Henkel Research Corp | Alkalisches proteolytisches enzym und verfahren zur herstellung |
| EP0877077B1 (en) | 1991-06-11 | 2010-05-26 | Genencor International, Inc. | Detergent composition containing cellulase compositions deficient in CBH I type components |
| DK82893D0 (da) * | 1993-07-08 | 1993-07-08 | Novo Nordisk As | Peptid |
| ATE512227T1 (de) * | 1994-02-24 | 2011-06-15 | Henkel Ag & Co Kgaa | Verbesserte enzyme und detergentien damit |
| WO1996029397A1 (en) | 1995-03-17 | 1996-09-26 | Novo Nordisk A/S | Novel endoglucanases |
| ES2525677T3 (es) | 1995-10-17 | 2014-12-29 | Ab Enzymes Oy | Celulasas, genes que las codifican y usos de las mismas |
| CN100362100C (zh) | 1996-09-17 | 2008-01-16 | 诺沃奇梅兹有限公司 | 纤维素酶变体 |
| DE19713852A1 (de) | 1997-04-04 | 1998-10-08 | Henkel Kgaa | Aktivatoren für Persauerstoffverbindungen in Wasch- und Reinigungsmitteln |
| ES2532606T3 (es) | 1999-03-31 | 2015-03-30 | Novozymes A/S | Polipéptidos con actividad de alfa-amilasa alcalina y ácidos nucleicos que los codifican |
| AR027073A1 (es) | 1999-12-23 | 2003-03-12 | Upjohn Co | Ensayos y metodos de diagnostico y tratamiento basados en el uso de canales de sodio como objetivos de amiloide beta o de sus agregados |
| ATE481492T1 (de) | 2000-08-04 | 2010-10-15 | Genencor Int | Mutierte trichoderma reesei egiii cellulasen, dafür kodierende dna und verfahren zu deren herstellung |
| JP4242761B2 (ja) | 2001-06-06 | 2009-03-25 | ノボザイムス アクティーゼルスカブ | エンド−β−１，４−グルカナーゼ |
| DE10131441A1 (de) | 2001-06-29 | 2003-01-30 | Henkel Kgaa | Eine neue Gruppe von alpha-Amylasen sowie ein Verfahren zur Identifizierung und Gewinnung neuer alpha-Amylasen |
| DE10163748A1 (de) | 2001-12-21 | 2003-07-17 | Henkel Kgaa | Neue Glykosylhydrolasen |
| DK1523553T3 (da) * | 2002-01-16 | 2010-04-19 | Genencor Int | Multi-substituerede proteasevarianter |
| DE10260903A1 (de) | 2002-12-20 | 2004-07-08 | Henkel Kgaa | Neue Perhydrolasen |
| DE10309555A1 (de) * | 2003-03-04 | 2004-09-23 | Henkel Kgaa | Faktor PrfB (Peptide chain release factor B) aus Bacillus licheniformis und Steigerung der Proteinbildungsrate durch Erhöhung der PrfB-Aktivität |
| CN103333870A (zh) | 2003-12-03 | 2013-10-02 | 丹尼斯科美国公司 | 过水解酶 |
| DE102004013843A1 (de) * | 2004-03-20 | 2005-10-13 | Degussa Ag | Expression von Nitrilhydratasen im Zwei-Vektor-Expressionssystem |
| DE102004029475A1 (de) | 2004-06-18 | 2006-01-26 | Henkel Kgaa | Neues enzymatisches Bleichsystem |
| US20080199443A1 (en) * | 2005-06-09 | 2008-08-21 | The Govt. Of The U.S.A. As Represented By The Secretary, Dept. Of Health And Human Services | Bone Morphogenetic Variants, Compositions and Methods of Treatment |
| DE102006038448A1 (de) | 2005-12-28 | 2008-02-21 | Henkel Kgaa | Enzym-haltiges Reinigungsmittel |
| WO2007122175A1 (en) * | 2006-04-20 | 2007-11-01 | Novozymes A/S | Savinase variants having an improved wash performance on egg stains |
| DE102006022224A1 (de) | 2006-05-11 | 2007-11-15 | Henkel Kgaa | Subtilisin aus Bacillus pumilus und Wasch- und Reinigungsmittel enthaltend dieses neue Subtilisin |
| EP1873251A1 (en) | 2006-06-29 | 2008-01-02 | Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus | Expression vector(s) for enhanced expression of a protein of interest in eukaryotic or prokaryotic host cells |
| KR20150115023A (ko) * | 2006-07-18 | 2015-10-13 | 다니스코 유에스 인크. | 광범위한 온도에 걸쳐 활성인 프로테아제 변이형 |
| DE102007003143A1 (de) | 2007-01-16 | 2008-07-17 | Henkel Kgaa | Neue Alkalische Protease aus Bacillus gibsonii und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease |
| AU2008348270A1 (en) * | 2007-12-21 | 2009-07-30 | Danisco Us Inc. | Enhanced protein production in bacillus |
-
2011
- 2011-04-13 DE DE102011007313A patent/DE102011007313A1/de not_active Withdrawn
-
2012
- 2012-04-05 DK DK12713714.9T patent/DK2697376T3/en active
- 2012-04-05 US US14/110,724 patent/US9663798B2/en not_active Expired - Fee Related
- 2012-04-05 KR KR1020137029683A patent/KR20140019436A/ko not_active Ceased
- 2012-04-05 PL PL12713714T patent/PL2697376T3/pl unknown
- 2012-04-05 ES ES12713714.9T patent/ES2639114T3/es active Active
- 2012-04-05 RU RU2013150276A patent/RU2642324C2/ru not_active IP Right Cessation
- 2012-04-05 WO PCT/EP2012/056262 patent/WO2012139964A1/de not_active Ceased
- 2012-04-05 MX MX2013011816A patent/MX348766B/es active IP Right Grant
- 2012-04-05 EP EP12713714.9A patent/EP2697376B1/de not_active Not-in-force
- 2012-04-05 CA CA2831473A patent/CA2831473A1/en not_active Abandoned
- 2012-04-05 CN CN201280028836.1A patent/CN103608457A/zh active Pending
- 2012-04-05 JP JP2014504258A patent/JP6099627B2/ja not_active Expired - Fee Related
- 2012-04-05 BR BR112013025951A patent/BR112013025951A2/pt not_active Application Discontinuation
-
2016
- 2016-12-15 JP JP2016243563A patent/JP2017079768A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012139964A1 (de) | 2012-10-18 |
| ES2639114T3 (es) | 2017-10-25 |
| DK2697376T3 (en) | 2017-09-18 |
| RU2013150276A (ru) | 2015-05-20 |
| MX2013011816A (es) | 2013-10-25 |
| CN103608457A (zh) | 2014-02-26 |
| JP2014511702A (ja) | 2014-05-19 |
| JP6099627B2 (ja) | 2017-03-22 |
| US20140220628A1 (en) | 2014-08-07 |
| EP2697376B1 (de) | 2017-05-31 |
| BR112013025951A2 (pt) | 2017-08-01 |
| US9663798B2 (en) | 2017-05-30 |
| JP2017079768A (ja) | 2017-05-18 |
| DE102011007313A1 (de) | 2012-10-18 |
| KR20140019436A (ko) | 2014-02-14 |
| RU2642324C2 (ru) | 2018-01-24 |
| CA2831473A1 (en) | 2012-10-18 |
| MX348766B (es) | 2017-06-28 |
| EP2697376A1 (de) | 2014-02-19 |
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