PL2764016T3 - Regulowany promotor - Google Patents

Regulowany promotor

Info

Publication number
PL2764016T3
PL2764016T3 PL12768859T PL12768859T PL2764016T3 PL 2764016 T3 PL2764016 T3 PL 2764016T3 PL 12768859 T PL12768859 T PL 12768859T PL 12768859 T PL12768859 T PL 12768859T PL 2764016 T3 PL2764016 T3 PL 2764016T3
Authority
PL
Poland
Prior art keywords
regulatable promoter
regulatable
promoter
Prior art date
Application number
PL12768859T
Other languages
English (en)
Inventor
Diethard Mattanovich
Brigitte Gasser
Michael Maurer
Roland PRIELHOFER
Joachim Klein
Jana Wenger
Original Assignee
Lonza Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza Ltd filed Critical Lonza Ltd
Publication of PL2764016T3 publication Critical patent/PL2764016T3/pl

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0008Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y102/00Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01012Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (1.2.1.12)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55Fab or Fab'
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
PL12768859T 2011-10-07 2012-10-05 Regulowany promotor PL2764016T3 (pl)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161544451P 2011-10-07 2011-10-07
EP11184323 2011-10-07
EP12171006 2012-06-06
EP12768859.6A EP2764016B1 (en) 2011-10-07 2012-10-05 Regulatable promoter
PCT/EP2012/069757 WO2013050551A1 (en) 2011-10-07 2012-10-05 Regulatable promoter

Publications (1)

Publication Number Publication Date
PL2764016T3 true PL2764016T3 (pl) 2019-08-30

Family

ID=48043194

Family Applications (1)

Application Number Title Priority Date Filing Date
PL12768859T PL2764016T3 (pl) 2011-10-07 2012-10-05 Regulowany promotor

Country Status (17)

Country Link
US (4) US9512432B2 (pl)
EP (2) EP3508494B1 (pl)
JP (1) JP6113736B2 (pl)
KR (2) KR101954389B1 (pl)
CN (3) CN110055248A (pl)
AR (1) AR088252A1 (pl)
AU (1) AU2012320421B2 (pl)
BR (1) BR112014008152A2 (pl)
CA (1) CA2849856C (pl)
DK (1) DK3508494T3 (pl)
ES (1) ES2913825T3 (pl)
IL (1) IL231859B (pl)
IN (1) IN2014DN03419A (pl)
PL (1) PL2764016T3 (pl)
SG (2) SG10201602673PA (pl)
TW (1) TWI560272B (pl)
WO (1) WO2013050551A1 (pl)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3508494T3 (da) * 2011-10-07 2022-05-23 Lonza Ag Regulerbar promotor
CN104195165A (zh) * 2014-09-01 2014-12-10 北京爱必信生物技术有限公司 包含葡萄糖启动子的载体、宿主菌以及重组牛源胰蛋白酶的制备方法
EP3217807A4 (en) 2014-11-11 2018-09-12 Clara Foods Co. Methods and compositions for egg white protein production
DK3332005T3 (da) * 2015-08-05 2022-01-03 Lonza Ag Promotorvarianter
CN109219662A (zh) 2016-03-02 2019-01-15 龙沙有限公司 改进的发酵工艺
CN110088279A (zh) * 2016-12-15 2019-08-02 株式会社钟化 新型宿主细胞及使用了其的目标蛋白质的制造方法
JP7717459B2 (ja) * 2018-02-12 2025-08-04 ロンザ リミテッド 目的タンパク質を産生するための宿主細胞
EP3536784A1 (en) 2018-03-05 2019-09-11 ACIB GmbH Host cell engineered for improved metabolite production
US11866714B2 (en) 2018-06-07 2024-01-09 Basf Se Promoter for yeast
JP7061234B2 (ja) * 2019-01-11 2022-04-27 ロンザ リミテッド 組換え宿主細胞内での炭素源調節タンパク質産生
HRP20230560T1 (hr) * 2019-01-29 2023-08-18 Clariant Produkte (Deutschland) Gmbh Kimerni promotor za upotrebu u metaboličkom inženjeringu
WO2020200414A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Protein production in mut-methylotrophic yeast
ES2964319T3 (es) 2019-04-01 2024-04-05 Univ Wien Bodenkultur Levadura metilotrófica Mut-
WO2020200415A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Mut- methylotrophic yeast
CN114375304A (zh) 2019-07-11 2022-04-19 克莱拉食品公司 蛋白质组合物及其食用品
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
EP4127180A1 (en) 2020-03-26 2023-02-08 Asklepios Biopharmaceutical, Inc. Forskolin-inducible promoters and hypoxia-inducible promoters
US12529088B2 (en) 2020-04-01 2026-01-20 Lonza Ltd Helper factors for expressing proteins in yeast
US20230167458A1 (en) 2020-04-15 2023-06-01 Asklepios Biopharmaceutical, Inc. Forskolin-inducible promoters and hypoxia-inducible promoters
EP4222266A1 (en) 2020-09-30 2023-08-09 Lonza Ltd. Host cells overexpressing translational factors
CA3205520A1 (en) 2021-02-12 2022-08-18 Richard ZAHRL Signal peptides for increased protein secretion
WO2024133538A1 (en) 2022-12-20 2024-06-27 Lonza Ltd Host cells with pat1 knockout for increased specific protein productivity
WO2025247966A1 (en) 2024-05-29 2025-12-04 Mybios Gmbh Process for protein production in methylotrophic yeast host cell.
WO2026027251A1 (en) 2024-08-01 2026-02-05 Boehringer Ingelheim Rcv Gmbh & Co Kg Signal peptides for increased protein secretion

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU577259B2 (en) 1982-08-13 1988-09-22 Zymogenetics Inc. Glycolytic promters for regulated protein expression protease inhibitor
US4855231A (en) 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
US4808537A (en) 1984-10-30 1989-02-28 Phillips Petroleum Company Methanol inducible genes obtained from pichia and methods of use
WO1997044470A1 (en) 1996-05-21 1997-11-27 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
SE9802333D0 (sv) 1998-06-29 1998-06-29 Astra Pharma Prod Novel combination
US6730499B1 (en) 1998-07-03 2004-05-04 Research Corporation Technologies, Inc. Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1
US7259255B2 (en) 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
EP1773861A4 (en) * 2004-07-22 2008-04-16 Glycofi Inc MALATE SYNTHESIS REGULATOR SEQUENCES FOR HETEROLOGIC GENE EXPRESSION IN PICHIA
AT501955B1 (de) 2005-02-23 2007-08-15 Univ Graz Tech Mutierte aox1-promotoren
WO2007058407A1 (en) 2005-11-16 2007-05-24 Korea Research Institute Of Bioscience & Biotechnology Translational elongation factor promoter from pichia pastoris and method for producing recombinant protein using the same
KR100717353B1 (ko) 2006-04-12 2007-05-11 한국생명공학연구원 피키아 파스토리스 유래의 자동 유도성 nps 프로모터 및이를 이용한 이종 단백질의 제조방법
CN100552029C (zh) 2006-09-14 2009-10-21 南开大学 毕赤酵母ω3-脂肪酸脱氢酶启动子序列及其应用
US8222386B2 (en) 2006-10-10 2012-07-17 Keck Graduate Institute P. pastoris ADH promoter and use thereof to direct expression of proteins
JP2010524440A (ja) * 2007-04-20 2010-07-22 ポリマン サイエンティフィック イミューンバイオロジッシュ フォーシュング ゲゼルシャフト ミット ベシュレンクテル ファフツング 発現系
US8105811B2 (en) * 2008-06-13 2012-01-31 Wisconsin Alumni Research Foundation Sugar transport sequences, yeast strains having improved sugar uptake, and methods of use
US8440456B2 (en) 2009-05-22 2013-05-14 Vib, Vzw Nucleic acids of Pichia pastoris and use thereof for recombinant production of proteins
AP3766A (en) * 2009-07-09 2016-07-31 Du Pont Engineered microorganisms with enhanced fermentation activity
EP2292769A1 (en) * 2009-08-10 2011-03-09 Lonza AG Fermentation process
DK3508494T3 (da) * 2011-10-07 2022-05-23 Lonza Ag Regulerbar promotor
DK3332005T3 (da) 2015-08-05 2022-01-03 Lonza Ag Promotorvarianter

Also Published As

Publication number Publication date
DK3508494T3 (da) 2022-05-23
CN113788884A (zh) 2021-12-14
CA2849856C (en) 2023-03-14
US9512432B2 (en) 2016-12-06
SG10201602673PA (en) 2016-05-30
IL231859A0 (en) 2014-05-28
WO2013050551A1 (en) 2013-04-11
KR20190025048A (ko) 2019-03-08
US20220259608A1 (en) 2022-08-18
EP2764016B1 (en) 2019-03-13
JP6113736B2 (ja) 2017-04-12
US20190185866A1 (en) 2019-06-20
CN110055248A (zh) 2019-07-26
SG11201400811QA (en) 2014-08-28
ES2913825T3 (es) 2022-06-06
EP3508494A1 (en) 2019-07-10
AU2012320421A1 (en) 2014-03-20
US20170037418A1 (en) 2017-02-09
CA2849856A1 (en) 2013-04-11
TW201317350A (zh) 2013-05-01
CN113788884B (zh) 2024-07-23
CN103998460A (zh) 2014-08-20
KR101954389B1 (ko) 2019-03-06
AU2012320421B2 (en) 2017-03-02
US10301634B2 (en) 2019-05-28
EP3508494B1 (en) 2022-02-23
KR102128957B1 (ko) 2020-07-02
BR112014008152A2 (pt) 2017-04-18
US11976284B2 (en) 2024-05-07
US11401523B2 (en) 2022-08-02
IL231859B (en) 2020-08-31
JP2014530016A (ja) 2014-11-17
EP2764016A1 (en) 2014-08-13
TWI560272B (en) 2016-12-01
KR20140069112A (ko) 2014-06-09
US20140242636A1 (en) 2014-08-28
IN2014DN03419A (pl) 2015-05-22
AR088252A1 (es) 2014-05-21

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