PL3215619T3 - Sposób selektywnego oddzielania frakcji wielkości i izolowania mieszanin kwasów nukleinowych - Google Patents

Sposób selektywnego oddzielania frakcji wielkości i izolowania mieszanin kwasów nukleinowych

Info

Publication number
PL3215619T3
PL3215619T3 PL15837175.7T PL15837175T PL3215619T3 PL 3215619 T3 PL3215619 T3 PL 3215619T3 PL 15837175 T PL15837175 T PL 15837175T PL 3215619 T3 PL3215619 T3 PL 3215619T3
Authority
PL
Poland
Prior art keywords
isolation
size
nucleic acid
acid mixtures
selective separation
Prior art date
Application number
PL15837175.7T
Other languages
English (en)
Polish (pl)
Inventor
Timo Hillebrand
Original Assignee
Ist Innuscreen Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ist Innuscreen Gmbh filed Critical Ist Innuscreen Gmbh
Publication of PL3215619T3 publication Critical patent/PL3215619T3/pl

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00Processes for the preparation of sugar derivatives
    • C07H1/06Separation; Purification
    • C07H1/08Separation; Purification from natural products
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/119Reactions demanding special reaction conditions pH
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/137Metal/ion, e.g. metal label

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PL15837175.7T 2014-11-07 2015-11-09 Sposób selektywnego oddzielania frakcji wielkości i izolowania mieszanin kwasów nukleinowych PL3215619T3 (pl)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102014222810 2014-11-07
PCT/EP2015/076075 WO2016071535A1 (de) 2014-11-07 2015-11-09 Verfahren zur selektiven grössenfraktionierten trennung und isolierung von nukleinsäuremischungen

Publications (1)

Publication Number Publication Date
PL3215619T3 true PL3215619T3 (pl) 2023-05-22

Family

ID=55409797

Family Applications (1)

Application Number Title Priority Date Filing Date
PL15837175.7T PL3215619T3 (pl) 2014-11-07 2015-11-09 Sposób selektywnego oddzielania frakcji wielkości i izolowania mieszanin kwasów nukleinowych

Country Status (7)

Country Link
US (1) US10519435B2 (es)
EP (1) EP3215619B1 (es)
CN (1) CN107532165B (es)
DE (1) DE102015222002A1 (es)
ES (1) ES2940905T3 (es)
PL (1) PL3215619T3 (es)
WO (1) WO2016071535A1 (es)

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2067711C (en) 1991-05-03 2000-08-08 Daniel Lee Woodard Solid phase extraction purification of dna
AU2001238616A1 (en) 2000-02-23 2001-09-03 Wen Shao Rapid nucleic acid separation, isolation and purification methods
US6887668B2 (en) * 2002-04-19 2005-05-03 Beckman Coulter, Inc. Nucleic acid separation and detection by electrophoresis with a counter-migrating high-affinity intercalating dye
EP1560926B2 (de) 2002-11-08 2013-08-21 STRATEC Molecular GmbH Neuartige pufferformulierungen zur isolierung, reinigung und rückgewinnung lang- und kurzkettiger nukleinsäuren
DE10253351B4 (de) 2002-11-08 2007-02-22 InViTek Gesellschaft für Biotechnik & Biodesign mbH Neuartige Pufferfomulierungen zur Isolierung, Reinigung und Rückgewinnung lang- und kurzkettiger Nukleinsäuren
DE102005047736B4 (de) 2005-09-29 2008-08-14 Aj Innuscreen Gmbh Verfahren und System zur Isolierung von Nukleinsäuren aus beliebigen komplexen Ausgangsmaterialien
JP4699868B2 (ja) 2005-11-04 2011-06-15 株式会社日立ハイテクノロジーズ 核酸精製方法及び核酸精製器具
DE102005057334A1 (de) 2005-11-28 2007-06-06 Aj Innuscreen Gmbh Verfahren zur Isolierung von Nukleinsäuren aus beliebigen Ausgangsmaterialien
DE102005059217B4 (de) 2005-12-07 2011-03-17 Aj Innuscreen Gmbh Verfahren und Testkit zur Trennung, Aufreinigung und Wiedergewinnung von lang- und kurzkettigen Nukleinsäuren
WO2009055596A2 (en) 2007-10-23 2009-04-30 Cedars-Sinai Medical Center Methods of using genetic variants to diagnose and predict metabolic syndrome and associated traits
EP2128169A1 (de) 2008-05-30 2009-12-02 Qiagen GmbH Verfahren zur Isolierung von kurzkettigen Nukleinsäuren
WO2013045434A1 (en) * 2011-09-26 2013-04-04 Qiagen Gmbh Methods for separating nucleic acids by size
WO2013181651A1 (en) 2012-06-01 2013-12-05 Omega Bio-Tek, Inc. Selective nucleic acid fragment recovery
EP2954054B1 (en) * 2013-02-08 2018-12-05 Qiagen GmbH Method for separating dna by size

Also Published As

Publication number Publication date
US20180208923A1 (en) 2018-07-26
ES2940905T3 (es) 2023-05-12
CN107532165B (zh) 2021-04-09
CN107532165A (zh) 2018-01-02
EP3215619A1 (de) 2017-09-13
DE102015222002A1 (de) 2016-05-12
US10519435B2 (en) 2019-12-31
EP3215619B1 (de) 2023-01-04
WO2016071535A1 (de) 2016-05-12

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