PL3481942T3 - Sposoby hodowania organoidów - Google Patents

Sposoby hodowania organoidów

Info

Publication number
PL3481942T3
PL3481942T3 PL17742271T PL17742271T PL3481942T3 PL 3481942 T3 PL3481942 T3 PL 3481942T3 PL 17742271 T PL17742271 T PL 17742271T PL 17742271 T PL17742271 T PL 17742271T PL 3481942 T3 PL3481942 T3 PL 3481942T3
Authority
PL
Poland
Prior art keywords
methods
growing organoids
organoids
growing
Prior art date
Application number
PL17742271T
Other languages
English (en)
Inventor
Marianne J ELLIS
Julian CHAUDHURI
Trevor Clive Dale
Original Assignee
Cellesce Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellesce Limited filed Critical Cellesce Limited
Publication of PL3481942T3 publication Critical patent/PL3481942T3/pl

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0693Tumour cells; Cancer cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081Purging biological preparations of unwanted cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0679Cells of the gastro-intestinal tract
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062General methods for three-dimensional culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00Methods for the dissociation of cells, e.g. specific use of enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10Mechanical dissociation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/003D culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/50Proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/90Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Oncology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
PL17742271T 2016-07-11 2017-07-11 Sposoby hodowania organoidów PL3481942T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1611982.8A GB201611982D0 (en) 2016-07-11 2016-07-11 Cell culture
PCT/GB2017/052026 WO2018011558A1 (en) 2016-07-11 2017-07-11 Methods for culturing organoids
EP17742271.4A EP3481942B1 (en) 2016-07-11 2017-07-11 Methods for culturing organoids

Publications (1)

Publication Number Publication Date
PL3481942T3 true PL3481942T3 (pl) 2022-06-20

Family

ID=56890801

Family Applications (1)

Application Number Title Priority Date Filing Date
PL17742271T PL3481942T3 (pl) 2016-07-11 2017-07-11 Sposoby hodowania organoidów

Country Status (11)

Country Link
US (3) US11401501B2 (pl)
EP (2) EP4056679A1 (pl)
JP (1) JP7123412B2 (pl)
CN (2) CN117402826A (pl)
CA (1) CA3028111A1 (pl)
DK (1) DK3481942T3 (pl)
ES (1) ES2910714T3 (pl)
GB (1) GB201611982D0 (pl)
PL (1) PL3481942T3 (pl)
PT (1) PT3481942T (pl)
WO (1) WO2018011558A1 (pl)

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WO2011140441A2 (en) 2010-05-06 2011-11-10 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
EP3712254A1 (en) 2014-05-28 2020-09-23 Children's Hospital Medical Center Methods and systems for converting precursor cells into gastric tissues through directed differentiation
CA2963704A1 (en) 2014-10-17 2016-04-21 Children's Hospital Medical Center In vivo model of human small intestine using pluripotent stem cells and methods of making and using same
EP4177335A1 (en) 2016-05-05 2023-05-10 Children's Hospital Medical Center Methods for the in vitro manufacture of gastric fundus tissue and compositions related to same
SG11201903697WA (en) 2016-11-04 2019-05-30 Childrens Hospital Med Ct Liver organoid compositions and methods of making and using same
EP3548507A4 (en) 2016-12-05 2020-07-15 Children's Hospital Medical Center COLON ORGANOIDS AND PROCESSES FOR THE PREPARATION AND USE THEREOF
EP3609997A4 (en) 2017-04-14 2021-03-03 Children's Hospital Medical Center COMPOSITIONS OF STEM CELLS FROM MULTIPLE DONOR CELLS AND THEIR PREPARATION PROCEDURES
US12297457B2 (en) 2017-10-10 2025-05-13 Children's Hospital Medical Center Esophageal tissue and/or organoid compositions and methods of making same
EP3727394A4 (en) 2017-12-21 2021-09-08 Children's Hospital Medical Center DIGITALIZED HUMAN ORGANOIDS AND METHOD OF USING THEREOF
US12421500B2 (en) 2018-07-26 2025-09-23 Children's Hospital Medical Center Hepato-biliary-pancreatic tissues and methods of making same
JP2021535753A (ja) 2018-09-12 2021-12-23 チルドレンズ ホスピタル メディカル センター 造血幹細胞およびその派生体の生成のためのオルガノイド組成物
CN109321528A (zh) * 2018-09-18 2019-02-12 梁廷波 中国人B7-H5表达阳性胰腺癌细胞系sipanc-1076建立方法
JP2022514298A (ja) * 2018-12-19 2022-02-10 リージェンツ オブ ザ ユニバーシティ オブ ミネソタ オルガノイドおよびその組織を作製および使用するための方法
WO2020160371A1 (en) 2019-02-01 2020-08-06 The University Of Hong Kong Innervated organoid compositions and methods of making same
JP7501875B2 (ja) * 2019-02-28 2024-06-18 公立大学法人横浜市立大学 血液凝固および/または補体異常疾患の治療用組成物
CA3141814A1 (en) 2019-05-31 2020-12-03 Children's Hospital Medical Center Shaped organoid compositions and methods of making same
CN114174494A (zh) 2019-05-31 2022-03-11 儿童医院医学中心 产生和扩增造血干细胞的方法
WO2020255546A1 (ja) 2019-06-20 2020-12-24 Jsr株式会社 細胞塊群の製造方法および製造装置
KR102213931B1 (ko) * 2019-09-16 2021-02-05 포항공과대학교 산학협력단 줄기세포 또는 종양세포 기반 다종세포 조직 구조 모사체 및 이의 제조 방법
WO2021261622A1 (ko) * 2020-06-25 2021-12-30 주식회사 넥스트앤바이오 표준형 오가노이드 제조방법
US12606783B2 (en) 2020-06-25 2026-04-21 Next & Bio Inc. Well plate and 3D culture plate comprising the same
US12540308B2 (en) 2020-06-25 2026-02-03 Next & Bio Inc. Method for mass proliferation of stem cells without using hydrogel
CN112300933B (zh) * 2020-10-30 2023-10-03 广州迈普再生医学科技股份有限公司 类器官成型装置及方法
KR102746470B1 (ko) 2021-07-09 2024-12-26 주식회사 넥스트앤바이오 가상 오가노이드 생성 방법
KR102746473B1 (ko) 2021-07-09 2024-12-26 주식회사 넥스트앤바이오 가상 오가노이드를 이용한 촬영 이미지 데이터 생성 방법
CN114908032B (zh) * 2022-03-18 2024-01-09 暨南大学 一种类睾丸器官的制备、培养、冻存复苏方法及应用
US20240392250A1 (en) * 2022-05-24 2024-11-28 Bioarchitec Group Limited High-throughput selection and fabrication of biomaterial-encapsulated cell mass and uses thereof
CN115261302B (zh) * 2022-07-20 2023-06-06 创芯国际生物科技(广州)有限公司 一种基质胶及其制备方法和应用
CN115537376A (zh) * 2022-10-27 2022-12-30 四川大学华西第二医院 一种睾丸类器官及其构建方法与应用
CN115418386B (zh) * 2022-11-03 2023-03-24 成都诺医德医学检验实验室有限公司 一种基于皮肤类器官的损伤愈合评价方法及模型和应用
GB202306791D0 (en) 2023-05-09 2023-06-21 Cellesce Ltd Apparatus
GB202306769D0 (en) 2023-05-09 2023-06-21 Cellesce Ltd Composition
CN117050934B (zh) * 2023-10-11 2024-01-30 四川大学华西医院 小鼠前列腺类器官制备方法及原发原位前列腺癌动物模型

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WO2013063588A1 (en) * 2011-10-28 2013-05-02 The Board Of Trustees Of The Leland Stanford Junior University Ex vivo culture, proliferation and expansion of primary tissue organoids
WO2013159939A1 (en) * 2012-04-26 2013-10-31 Technische Universität Berlin Methods for determining and/or monitoring conditions of a three dimensional cell culture system and optical sensor device for conducting said methods
JP2014233209A (ja) 2013-05-31 2014-12-15 株式会社古河電工アドバンストエンジニアリング 細胞分離方法および細胞分離装置
JP2015062392A (ja) 2013-09-26 2015-04-09 学校法人東京電機大学 毛細血管の製造方法および毛細血管の製造装置
JP6482052B2 (ja) 2014-02-10 2019-03-13 国立大学法人北陸先端科学技術大学院大学 細胞シート及び三次元細胞培養体の凍結保存のための緩慢ガラス化方法
WO2015173425A1 (en) * 2014-05-16 2015-11-19 Koninklijke Nederlandse Akademie Van Wetenschappen Improved culture method for organoids
GB201421094D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
GB201421092D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium

Also Published As

Publication number Publication date
DK3481942T3 (da) 2022-04-11
ES2910714T3 (es) 2022-05-13
US12351820B2 (en) 2025-07-08
US11401501B2 (en) 2022-08-02
CN117402826A (zh) 2024-01-16
PT3481942T (pt) 2022-04-05
US20190249137A1 (en) 2019-08-15
JP2019521707A (ja) 2019-08-08
CA3028111A1 (en) 2018-01-18
WO2018011558A1 (en) 2018-01-18
CN109844097B (zh) 2023-10-31
US20250368952A1 (en) 2025-12-04
CN109844097A (zh) 2019-06-04
EP3481942B1 (en) 2022-02-16
EP4056679A1 (en) 2022-09-14
EP3481942A1 (en) 2019-05-15
US20220333064A1 (en) 2022-10-20
JP7123412B2 (ja) 2022-08-23
GB201611982D0 (en) 2016-08-24

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