PL3620510T3 - Fermentacyjne wytwarzanie oligosacharydów przez całkowitą fermentację z wykorzystaniem mieszanego surowca - Google Patents

Fermentacyjne wytwarzanie oligosacharydów przez całkowitą fermentację z wykorzystaniem mieszanego surowca

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Publication number
PL3620510T3
PL3620510T3 PL18192898.7T PL18192898T PL3620510T3 PL 3620510 T3 PL3620510 T3 PL 3620510T3 PL 18192898 T PL18192898 T PL 18192898T PL 3620510 T3 PL3620510 T3 PL 3620510T3
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PL
Poland
Prior art keywords
oligosaccharides
fermentative production
total fermentation
mixed feedstock
fermentation utilizing
Prior art date
Application number
PL18192898.7T
Other languages
English (en)
Inventor
Stefan Jennewein
Dirk Wartenberg
Katja Parschat
Original Assignee
Chr. Hansen HMO GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=63524125&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL3620510(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Chr. Hansen HMO GmbH filed Critical Chr. Hansen HMO GmbH
Publication of PL3620510T3 publication Critical patent/PL3620510T3/pl

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/0109—N-Acetyllactosamine synthase (2.4.1.90)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01056—1-Phosphofructokinase (2.7.1.56)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03002—UDP-glucose 4-epimerase (5.1.3.2), i.e. UDP-galactose 4-epimerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/02—Phosphotransferases (phosphomutases) (5.4.2)
    • C12Y504/02002—Phosphoglucomutase (5.4.2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01038—Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (2.4.1.38)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01004—Fructokinase (2.7.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01011—6-Phosphofructokinase (2.7.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07009—UTP-glucose-1-phosphate uridylyltransferase (2.7.7.9), i.e. UDP-glucose-pyrophosphorylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
    • C12Y301/03011—Fructose-bisphosphatase (3.1.3.11)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
PL18192898.7T 2018-09-06 2018-09-06 Fermentacyjne wytwarzanie oligosacharydów przez całkowitą fermentację z wykorzystaniem mieszanego surowca PL3620510T3 (pl)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP18192898.7A EP3620510B1 (en) 2018-09-06 2018-09-06 Fermentative production of oligosaccharides by total fermentation utilizing a mixed feedstock

Publications (1)

Publication Number Publication Date
PL3620510T3 true PL3620510T3 (pl) 2024-02-19

Family

ID=63524125

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PL18192898.7T PL3620510T3 (pl) 2018-09-06 2018-09-06 Fermentacyjne wytwarzanie oligosacharydów przez całkowitą fermentację z wykorzystaniem mieszanego surowca

Country Status (12)

Country Link
US (2) US11713475B2 (pl)
EP (2) EP3620510B1 (pl)
JP (1) JP7635118B2 (pl)
KR (1) KR102913982B1 (pl)
CN (1) CN112654697B (pl)
AU (1) AU2019336311A1 (pl)
BR (1) BR112021003502A2 (pl)
ES (1) ES2965229T3 (pl)
PH (1) PH12021550473A1 (pl)
PL (1) PL3620510T3 (pl)
SG (1) SG11202101554VA (pl)
WO (1) WO2020048927A1 (pl)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2965229T3 (es) 2018-09-06 2024-04-11 Chr Hansen Hmo Gmbh Producción fermentativa de oligosacáridos mediante fermentación total usando una materia prima mixta
EP3702468A1 (en) 2019-03-01 2020-09-02 Jennewein Biotechnologie GmbH Fermentative production of carbohydrates by microbial cells utilizing a mixed feedstock
FR3110176B1 (fr) * 2020-05-12 2022-05-13 Institut Nat Des Sciences Appliquees De Toulouse Souche et procédé de production d’oligosaccharides
JP7810443B2 (ja) * 2020-08-19 2026-02-03 コナゲン インコーポレイテッド ２－フコシルラクトースの生合成産生
CN112574936A (zh) * 2020-12-21 2021-03-30 中国科学院合肥物质科学研究院 一种重组大肠杆菌及其构建方法与应用
KR102633804B1 (ko) * 2020-12-31 2024-02-05 주식회사 삼양사 재조합 바실러스 속 미생물 및 이를 이용한 모유올리고당 제조방법
KR102793833B1 (ko) * 2020-12-31 2025-04-11 주식회사 삼양사 푸코오스 전이효소를 발현하는 재조합 미생물 및 이를 이용한 2’-푸코실락토오스 제조방법
BR112023017146A2 (pt) * 2021-02-26 2023-09-26 Chr Hansen As Método de produção de alolactose
CN113136357B (zh) * 2021-04-25 2022-10-11 江南大学 一种产乳酰-n-新四糖的基因工程菌及生产方法
WO2023057598A1 (en) * 2021-10-07 2023-04-13 Eligo Bioscience Methods involving bacterial strain replacement
CN115094103A (zh) * 2022-07-22 2022-09-23 南宁东恒华道生物科技有限责任公司 以蔗糖、淀粉或糖原为原料组合酶法生产半乳糖的新工艺
CN116064345A (zh) * 2022-08-16 2023-05-05 江南大学 高效生产岩藻糖基乳糖的无抗基因工程菌及其应用
KR20240109187A (ko) * 2022-12-30 2024-07-10 주식회사 삼양사 푸코실락토오스를 생산하는 재조합 미생물 및 이를 이용한 푸코실락토오스 생산방법
CN121752733A (zh) 2023-07-07 2026-03-27 因比奥斯公司 用于无动物乳糖合成的简单方法
KR20250153365A (ko) * 2024-04-17 2025-10-27 씨제이제일제당 (주) 프럭토키나제 변이체 및 이를 이용한 l-아미노산 생산 방법

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2796082B1 (fr) 1999-07-07 2003-06-27 Centre Nat Rech Scient Procede de production d'oligosaccharides
US20080145899A1 (en) 2004-09-17 2008-06-19 Neose Technologies Inc Production of Oligosaccharides By Microorganisms
KR101324369B1 (ko) 2008-11-05 2013-11-01 미쓰이 가가쿠 가부시키가이샤 2-디옥시-실로-이노소스（ｄｏｉ） 생산 세균 및 이를 이용한 2-디옥시-실로-이노소스（ｄｏｉ） 생산 방법
DK3235907T5 (da) * 2010-07-12 2024-10-14 Inbiose Nv Metabolisk modificerede organismer til produktion af bioprodukter med merværdi
AU2012351501B2 (en) * 2011-12-16 2018-06-14 Inbiose N.V. Mutant microorganisms to synthesize colanic acid, mannosylated and/or fucosylated oligosaccharides
CN104178443B (zh) * 2013-05-24 2017-04-12 中国科学院天津工业生物技术研究所 生产丁二酸的重组大肠杆菌及其应用
AU2014342020C1 (en) 2013-10-31 2019-09-05 Fred Hutchinson Cancer Research Center Modified hematopoietic stem/progenitor and non-T effector cells, and uses thereof
ES2715010T3 (es) * 2014-03-31 2019-05-31 Jennewein Biotechnologie Gmbh Fermentación total de oligosacáridos
CN113684233B (zh) 2014-06-27 2024-11-29 格礼卡姆股份公司 寡糖的制备
EP3315610B1 (en) 2016-10-29 2020-12-16 Jennewein Biotechnologie GmbH Process for the production of fucosylated oligosaccharides
ES2965229T3 (es) * 2018-09-06 2024-04-11 Chr Hansen Hmo Gmbh Producción fermentativa de oligosacáridos mediante fermentación total usando una materia prima mixta

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JP7635118B2 (ja) 2025-02-25
CN112654697A (zh) 2021-04-13
JP2021536259A (ja) 2021-12-27
ES2965229T3 (es) 2024-04-11
KR102913982B1 (ko) 2026-01-16
WO2020048927A1 (en) 2020-03-12
EP3847239A1 (en) 2021-07-14
US11713475B2 (en) 2023-08-01
SG11202101554VA (en) 2021-03-30
BR112021003502A2 (pt) 2021-05-18
US12338473B2 (en) 2025-06-24
KR20210057734A (ko) 2021-05-21
CN112654697B (zh) 2025-08-29
EP3620510C0 (en) 2023-11-01
EP3620510B1 (en) 2023-11-01
US20210317493A1 (en) 2021-10-14
AU2019336311A1 (en) 2021-03-25
PH12021550473A1 (en) 2021-11-22
US20230399669A1 (en) 2023-12-14
EP3620510A1 (en) 2020-03-11

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