PL3663411T3 - Sposoby do cyfrowego pcr - Google Patents

Sposoby do cyfrowego pcr

Info

Publication number
PL3663411T3
PL3663411T3 PL19198901T PL19198901T PL3663411T3 PL 3663411 T3 PL3663411 T3 PL 3663411T3 PL 19198901 T PL19198901 T PL 19198901T PL 19198901 T PL19198901 T PL 19198901T PL 3663411 T3 PL3663411 T3 PL 3663411T3
Authority
PL
Poland
Prior art keywords
methods
digital pcr
pcr
digital
Prior art date
Application number
PL19198901T
Other languages
English (en)
Inventor
Mark Davies
Tara Dalton
Original Assignee
Stokes Bio Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stokes Bio Limited filed Critical Stokes Bio Limited
Publication of PL3663411T3 publication Critical patent/PL3663411T3/pl

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0832Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838Capillaries
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867Multiple inlets and one sample wells, e.g. mixing, dilution
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PL19198901T 2008-08-12 2009-08-11 Sposoby do cyfrowego pcr PL3663411T3 (pl)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US8814208P 2008-08-12 2008-08-12
PCT/IB2009/007568 WO2010018465A2 (en) 2008-08-12 2009-08-11 Methods and devices for digital pcr
EP19198901.1A EP3663411B1 (en) 2008-08-12 2009-08-11 Methods for digital pcr
EP09804312.8A EP2321427B1 (en) 2008-08-12 2009-08-11 Methods for digital pcr
EP17204051.1A EP3358019B1 (en) 2008-08-12 2009-08-11 Methods for digital pcr

Publications (1)

Publication Number Publication Date
PL3663411T3 true PL3663411T3 (pl) 2022-02-07

Family

ID=41669400

Family Applications (1)

Application Number Title Priority Date Filing Date
PL19198901T PL3663411T3 (pl) 2008-08-12 2009-08-11 Sposoby do cyfrowego pcr

Country Status (9)

Country Link
US (4) US9631230B2 (pl)
EP (4) EP4008795A1 (pl)
JP (1) JP2011530305A (pl)
CN (1) CN102232114A (pl)
DK (1) DK3663411T3 (pl)
ES (1) ES2902894T3 (pl)
PL (1) PL3663411T3 (pl)
PT (1) PT3663411T (pl)
WO (1) WO2010018465A2 (pl)

Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2363205A3 (en) 2006-01-11 2014-06-04 Raindance Technologies, Inc. Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
EP2530168B1 (en) 2006-05-11 2015-09-16 Raindance Technologies, Inc. Microfluidic Devices
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
PL3663411T3 (pl) 2008-08-12 2022-02-07 Stokes Bio Limited Sposoby do cyfrowego pcr
US9921154B2 (en) 2011-03-18 2018-03-20 Bio-Rad Laboratories, Inc. Multiplexed digital assays
US20130059754A1 (en) * 2011-09-01 2013-03-07 Bio-Rad Laboratories, Inc. Digital assays with reduced measurement uncertainty
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
CA2738578C (en) 2008-09-23 2021-05-04 Quantalife, Inc. Droplet-based assay system
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
CA2767056C (en) 2009-09-02 2018-12-04 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
JP5934657B2 (ja) 2010-02-12 2016-06-15 レインダンス テクノロジーズ, インコーポレイテッド デジタル検体分析
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
EP2556170A4 (en) 2010-03-25 2014-01-01 Quantalife Inc TRAPPING TRANSPORT AND DETECTION SYSTEM
CA2767113A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Detection system for droplet-based assays
CA2767182C (en) 2010-03-25 2020-03-24 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
EP2436444A1 (en) 2010-10-01 2012-04-04 Centre National de la Recherche Scientifique (C.N.R.S.) Microfluidic device for production and collection of droplets of a fluid
SG190074A1 (en) 2010-11-01 2013-06-28 Bio Rad Laboratories System for forming emulsions
JP6105485B2 (ja) * 2011-01-05 2017-04-05 ザ・チャイニーズ・ユニバーシティー・オブ・ホンコンThe Chinese University Of Hong Kong 胎児の性染色体遺伝子型の非侵襲的出生前同定
EP2673380B1 (en) 2011-02-09 2018-12-12 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
EP2675819B1 (en) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
AU2012231098B2 (en) 2011-03-18 2016-09-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
WO2012149042A2 (en) 2011-04-25 2012-11-01 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
DE202012013668U1 (de) 2011-06-02 2019-04-18 Raindance Technologies, Inc. Enzymquantifizierung
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2013019751A1 (en) 2011-07-29 2013-02-07 Bio-Rad Laboratories, Inc., Library characterization by digital assay
WO2013101741A1 (en) 2011-12-30 2013-07-04 Abbott Molecular, Inc. Channels with cross-sectional thermal gradients
WO2013155531A2 (en) 2012-04-13 2013-10-17 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
EP2844768B1 (en) * 2012-04-30 2019-03-13 Raindance Technologies, Inc. Digital analyte analysis
US9422602B2 (en) * 2012-08-15 2016-08-23 Bio-Rad Laboratories, Inc. Methods and compositions for determining nucleic acid degradation
US9970052B2 (en) 2012-08-23 2018-05-15 Bio-Rad Laboratories, Inc. Digital assays with a generic reporter
JP2016500856A (ja) 2012-09-14 2016-01-14 ライフ テクノロジーズ コーポレーション デジタルpcr実験設計器のための方法およびシステム
US9347056B2 (en) * 2012-10-26 2016-05-24 Seiko Epson Corporation Nucleic acid extraction device, and nucleic acid extraction method, nucleic acid extraction kit, and nucleic acid extraction apparatus, each using the same
CN105074060B (zh) 2013-02-01 2018-04-10 伯乐生命医学产品有限公司 用于靶水平的计算的使用数据排除的多重数字分析
CN105492455B (zh) * 2013-03-15 2019-05-03 伯乐生命医学产品有限公司 利用通用报告物的数字测定
US11952622B2 (en) * 2013-07-18 2024-04-09 The Johns Hopkins University Analysis of DNA-containing samples and resolution of mixed contributor DNA samples
CN105555972B (zh) * 2013-07-25 2020-07-31 伯乐生命医学产品有限公司 遗传测定
EP2878374B1 (en) * 2013-11-29 2021-05-12 IMEC vzw Method for performing digital PCR
CN103911427A (zh) * 2014-01-27 2014-07-09 上海涌泰生物医药科技有限公司 一种基于数字pcr平台检测基因点突变的方法和试剂盒
WO2015189336A1 (en) 2014-06-11 2015-12-17 Samplix S.A.R.L. Nucleotide sequence exclusion enrichment by droplet sorting (needls)
US10081017B2 (en) * 2014-10-08 2018-09-25 The Regents Of The University Of California Method and system for ultra-high dynamic range nucleic acid quantification
CA2967635C (en) * 2014-11-14 2023-06-06 Athena Diagnostics, Inc. Methods to detect a silent carrier genotype
CN104450891B (zh) * 2014-11-17 2017-06-06 中国科学院微生物研究所 基于微液滴的数字核酸扩增定量分析方法及系统
WO2016078339A1 (zh) 2014-11-17 2016-05-26 中国科学院微生物研究所 微液滴生成装置、系统、方法及单细胞/单分子分析装置
CN116497097A (zh) * 2015-09-29 2023-07-28 生命技术公司 用于执行数字pcr的系统和方法
JP6921638B2 (ja) 2017-06-16 2021-08-18 株式会社東芝 核酸検出定量方法、チップ、アッセイキット、核酸検出定量装置及びプログラム
US11072820B2 (en) 2017-10-19 2021-07-27 Bio-Rad Laboratories, Inc. Digital amplification assays with unconventional and/or inverse changes in photoluminescence
CN110835645B (zh) * 2018-08-16 2022-10-21 思纳福(北京)医疗科技有限公司 数字pcr检测方法
US12542195B2 (en) 2018-01-24 2026-02-03 Sniper (Suzhou) Life Technology Co., Ltd. Digital PCR detection apparatus, digital PCR quantitative detection method, multi-volume digital PCR quantitative analysis method, digital PCR detection method, nucleic acid detection microsphere, preparation method of nucleic acid detection microsphere, nucleic acid detection microsphere kit and high-throughput nucleic acid detection method
CN110066856A (zh) * 2018-01-24 2019-07-30 思纳福(北京)医疗科技有限公司 不同体积数字pcr定量分析方法
CN110066857B (zh) * 2018-01-24 2020-03-24 思纳福(北京)医疗科技有限公司 数字pcr定量检测方法
WO2019144905A1 (zh) 2018-01-24 2019-08-01 北京光阱管理咨询合伙企业(有限合伙) 微液滴容器、微液滴容器制备方法、微液滴平铺方法、微液滴生成试剂盒、温控装置、微液滴生成用油相组合物及其处理方法
CA3089393C (en) 2018-01-24 2023-04-04 Sniper (Beijing) Medical Technologies Co., Ltd Motion controlling mechanism, liquid discharging nozzle, microdroplet generating device and method, liquid driving mechanism and method, microdroplet generating method, and surface processing method of liquid discharging nozzle
TWI664292B (zh) * 2018-02-21 2019-07-01 奎克生技光電股份有限公司 用於低濃度核酸樣品的測量方法
CA3095588A1 (en) 2018-04-02 2019-10-10 Dropworks, Inc. Systems and methods for serial flow emulsion processes
WO2019226970A1 (en) 2018-05-24 2019-11-28 Dana-Farber Cancer Institute, Inc. Denaturation-enhanced dna mutation testing for limited biological specimens
CN113056551A (zh) 2018-11-16 2021-06-29 普雷斯基因组有限公司 用于产生液滴、核酸扩增和检测的集成微流控系统
US11358137B2 (en) 2018-12-26 2022-06-14 Industrial Technology Research Institute Tubular structure for producing droplets and method for producing droplets
EP4073573A4 (en) 2019-12-10 2024-04-10 Enumerix, Inc. Methods and systems for three-dimensional lightsheet imaging
CN117241878A (zh) 2021-03-05 2023-12-15 伊努梅里斯公司 用于生成微滴和执行数字分析的系统和方法
US12252745B2 (en) 2021-09-02 2025-03-18 Enumerix, Inc. Detection and digital quantitation of multiple targets
EP4436707A1 (en) 2021-12-01 2024-10-02 Vilnius University Microcapsules comprising biological samples, and methods for use of same
EP4453246A4 (en) 2021-12-20 2025-12-24 Countable Labs Inc DIGITAL DETECTION AND QUANTIFICATION OF MULTIPLE TARGETS
EP4549587A1 (en) 2023-11-02 2025-05-07 Institut Regional du Cancer de Montpellier Method for the diagnosis of a cancer with chromatin-bound mdm2

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
CA2159830C (en) 1994-04-29 2001-07-03 Applied Biosystems, Llc System for real time detection of nucleic acid amplification products
US6977145B2 (en) * 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor
CA2400601A1 (en) * 2001-08-31 2003-02-28 University Of Utah Research Foundation Real-time gene quantification with internal standards
EP1587940A4 (en) * 2002-12-20 2006-06-07 Caliper Life Sciences Inc SINGLE MOLECULAR AMPLIFICATION AND DNA PROOF
GB0315438D0 (en) * 2003-07-02 2003-08-06 Univ Manchester Analysis of mixed cell populations
KR100552706B1 (ko) * 2004-03-12 2006-02-20 삼성전자주식회사 핵산 증폭 방법 및 장치
KR101544351B1 (ko) 2005-02-18 2015-08-13 캐논 유.에스. 라이프 사이언시즈, 인크. 유기체의 게놈 dna를 확인하기 위한 장치 및 방법
HUE030215T2 (en) * 2006-02-02 2017-04-28 Univ Leland Stanford Junior Non-invasive fetal genetic screening by digital analysis
EP2298438A1 (en) 2006-02-07 2011-03-23 Stokes Bio Limited A microfluidic droplet queuing network
EP1991357B1 (en) 2006-02-07 2016-09-14 Stokes Bio Limited A microfluidic analysis system
EP2069070B1 (en) * 2006-09-28 2013-11-27 Stokes Bio Limited A qpcr analysis apparatus
US8931057B2 (en) 2006-10-24 2015-01-06 Avatier Corporation Apparatus and method for access validation
US9114398B2 (en) * 2006-11-29 2015-08-25 Canon U.S. Life Sciences, Inc. Device and method for digital multiplex PCR assays
PL3663411T3 (pl) 2008-08-12 2022-02-07 Stokes Bio Limited Sposoby do cyfrowego pcr
JP2014085228A (ja) 2012-10-24 2014-05-12 Fujitsu Ltd 携帯端末装置の制御方法、制御プログラム、携帯端末装置

Also Published As

Publication number Publication date
US20200283837A1 (en) 2020-09-10
ES2902894T3 (es) 2022-03-30
US10626451B2 (en) 2020-04-21
EP3663411B1 (en) 2021-11-24
CN102232114A (zh) 2011-11-02
EP3358019A1 (en) 2018-08-08
EP3663411A1 (en) 2020-06-10
JP2011530305A (ja) 2011-12-22
US20230295703A1 (en) 2023-09-21
DK3663411T3 (da) 2022-01-10
US20170356036A1 (en) 2017-12-14
EP2321427B1 (en) 2017-11-29
WO2010018465A3 (en) 2010-06-10
US20100092973A1 (en) 2010-04-15
EP3358019B1 (en) 2019-09-25
EP2321427A2 (en) 2011-05-18
PT3663411T (pt) 2022-01-14
US9631230B2 (en) 2017-04-25
WO2010018465A2 (en) 2010-02-18
EP4008795A1 (en) 2022-06-08

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