ATE335850T1 - Echtzeit-quantifizierung mit internen standards - Google Patents

Echtzeit-quantifizierung mit internen standards

Info

Publication number
ATE335850T1
ATE335850T1 AT02256006T AT02256006T ATE335850T1 AT E335850 T1 ATE335850 T1 AT E335850T1 AT 02256006 T AT02256006 T AT 02256006T AT 02256006 T AT02256006 T AT 02256006T AT E335850 T1 ATE335850 T1 AT E335850T1
Authority
AT
Austria
Prior art keywords
nucleic acid
mass fraction
real
pcr
internal standards
Prior art date
Application number
AT02256006T
Other languages
English (en)
Inventor
David J Eyre
Randy P Rasmussen
Brian E Caplin
Wade R Stevenson
Deepika Marine Desilva
Original Assignee
Univ Utah Res Found
Idaho Technology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Utah Res Found, Idaho Technology Inc filed Critical Univ Utah Res Found
Application granted granted Critical
Publication of ATE335850T1 publication Critical patent/ATE335850T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/708—Specific hybridization probes for papilloma

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AT02256006T 2001-08-31 2002-08-29 Echtzeit-quantifizierung mit internen standards ATE335850T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US31661401P 2001-08-31 2001-08-31

Publications (1)

Publication Number Publication Date
ATE335850T1 true ATE335850T1 (de) 2006-09-15

Family

ID=23229815

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02256006T ATE335850T1 (de) 2001-08-31 2002-08-29 Echtzeit-quantifizierung mit internen standards

Country Status (7)

Country Link
US (1) US7630837B2 (de)
EP (1) EP1288314B1 (de)
JP (1) JP4814479B2 (de)
AT (1) ATE335850T1 (de)
CA (1) CA2400601A1 (de)
DE (1) DE60213730T2 (de)
ES (1) ES2269614T3 (de)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7373253B2 (en) 2002-02-12 2008-05-13 Idaho Technology Multi-test analysis of real-time nucleic acid amplification
ATE384139T1 (de) * 2002-02-12 2008-02-15 Univ Utah Res Found Multi-test-analyse von echzeit- nukleinsäureamplifikationen
DE602004026111D1 (de) * 2003-12-19 2010-04-29 Nat Inst Of Advanced Ind Scien Verfahren zum testen von nukleinsäuren unter verwendung davon sowie dafür zu verwendende nukleinsäuresonde
US20050277121A1 (en) * 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
US20060008828A1 (en) * 2004-06-30 2006-01-12 Daisy Vanrompay Genotype specific detection of Chlamydophila psittaci
WO2006137830A2 (en) * 2004-08-09 2006-12-28 Marshfield Clinic Research Foundation Detection of mycobacterium
KR100738073B1 (ko) 2004-09-01 2007-07-12 삼성전자주식회사 실시간 핵산 증폭 데이터로부터 초기 핵산 농도를정량화하는 방법
JP4777631B2 (ja) * 2004-09-27 2011-09-21 株式会社日立ハイテクノロジーズ 核酸増幅分析法および装置
EP1921454B1 (de) * 2005-03-10 2015-08-12 Gen-Probe Incorporated Systeme und Verfahren zur Durchführung von Tests zum Nachweis oder zur Quantifizierung von Analyten
US7794659B2 (en) 2005-03-10 2010-09-14 Gen-Probe Incorporated Signal measuring system having a movable signal measuring device
GB2424886A (en) * 2005-04-04 2006-10-11 Dxs Ltd Polynucleotide primers against epidermal growth factor receptor and method of detecting gene mutations
US20060286587A1 (en) * 2005-06-15 2006-12-21 Gen-Probe Incorporated Methods for quantitative analysis of a nucleic acid amplification reaction
RU2005132940A (ru) * 2006-03-01 2007-11-27 Институт биохимии и генетики Уфимского научного центра РАН (RU) Способ детекции специфических фрагментов днк или рнк с помощью полимеразной цепной реакции в режиме реального времени
CA2627259C (en) * 2005-11-14 2016-02-23 Gen-Probe Incorporated Parametric calibration method
US20070148639A1 (en) * 2005-12-02 2007-06-28 Chagovetz Alexander M Methods and devices for nucleic acid amplification on a surface
US20070148677A1 (en) * 2005-12-02 2007-06-28 Chagovetz Alexander M Methods and systems for acquiring real-time quantitative melt data
WO2008070328A2 (en) * 2006-10-24 2008-06-12 Celera Corporation Systems and methods for baselining and real-time pcr data analysis
JP5638806B2 (ja) * 2006-11-03 2014-12-10 シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレーテッドＳｉｅｍｅｎｓ Ｈｅａｌｔｈｃａｒｅ Ｄｉａｇｎｏｓｔｉｃｓ Ｉｎｃ． ポリメラーゼ連鎖反応試験のためのランダムアクセスシステムおよび方法
AU2007325037B2 (en) * 2006-11-30 2012-09-20 Gen-Probe Incorporated Quantitative method employing adjustment of pre-defined master calibration curves
US20100285468A1 (en) * 2007-09-24 2010-11-11 Allelogic Biosciences Corporation Detection and/or quantification of nucleic acids
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
KR101401783B1 (ko) * 2007-12-26 2014-05-30 아크레이 가부시키가이샤 핵산 증폭 판정 방법 및 핵산 증폭 판정 장치
US9518288B2 (en) 2008-04-11 2016-12-13 University Of Utah Research Foundation Methods and compositions related to quantitative, array based methylation analysis
WO2010010951A1 (ja) * 2008-07-24 2010-01-28 Kuroda Masahiko 子宮内膜症関連疾患の指標ｍＲＮＡの発現の検出方法およびそれに用いるプローブ
WO2010018465A2 (en) 2008-08-12 2010-02-18 Stokes Bio Limited Methods and devices for digital pcr
EP2166470A1 (de) * 2008-09-19 2010-03-24 Corbett Research Pty Ltd Analyse von Schmelzkurven, insbesondere dsDNA- und Proteinschmelzkurven
ES2716807T3 (es) 2008-11-07 2019-06-17 Univ Utah Res Found Sesgo de amplificación de alelos
US8346485B2 (en) 2008-11-25 2013-01-01 Quest Diagnostics Investments Incorporated Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction
CA2749950C (en) * 2009-01-30 2018-01-09 Metcraft, Inc. Silverware, flatware or parts washer apparatus and method thereof
US8606527B2 (en) * 2009-02-27 2013-12-10 Bio-Rad Laboratories, Inc. SNP detection by melt curve clustering
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
BRPI1006682B1 (pt) * 2009-04-16 2020-04-22 Koninl Philips Electronics Nv método para a quantificação relativa de no mínimo um ácido nucleico alvo em uma amostra, método para a quantificação absoluta de no mínimo um ácido nucleico alvo em uma amostra, meios legíveis por máquina e equipamento para a análise das amostras de ácido nucleico
US20140141419A1 (en) * 2011-01-14 2014-05-22 Smiths Detection - Watford Limited Single tube quantitative polymerase chain reaction (pcr)
GB2506375B (en) 2012-09-27 2017-10-18 Epistem Ltd Data processing and analysis systems
WO2014142575A1 (en) * 2013-03-13 2014-09-18 Seegene, Inc. Quantification of target nucleic acid using melting peak analysis
US9976175B2 (en) 2013-03-15 2018-05-22 Gen-Probe Incorporated Calibration method, apparatus and computer program product
US10106841B2 (en) 2013-05-03 2018-10-23 Seegene, Inc. Quantification of target nucleic acid using common internal control
WO2016160823A1 (en) * 2015-04-03 2016-10-06 Becton, Dickinson And Company Methods of amplifying nucleic acids and compositions and kits for practicing the same
EP3130679B1 (de) * 2015-08-13 2018-02-28 Cladiac GmbH Verfahren und testsystem zum nachweis und/oder quantifizieren einer ziel-nukleinsäure in einer probe
US11332781B2 (en) 2016-06-23 2022-05-17 Canon U.S.A., Inc. Fitting melting curve data to determine copy number variation
CN114214395A (zh) * 2021-12-31 2022-03-22 上海星耀医学科技发展有限公司 一种提高基因检测准确性的核酸扩增方法与试剂盒

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5219727A (en) 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
US5455175A (en) 1990-06-04 1995-10-03 University Of Utah Research Foundation Rapid thermal cycling device
US5817462A (en) 1995-02-21 1998-10-06 Applied Spectral Imaging Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding
US5798262A (en) 1991-02-22 1998-08-25 Applied Spectral Imaging Ltd. Method for chromosomes classification
DE4137934A1 (de) 1991-11-18 1993-05-19 Boehringer Mannheim Gmbh Neue pentacyklische verbindungen und ihre verwendung als absorptions- oder fluoreszenzfarbstoffe
US5224775C2 (en) 1992-03-02 2002-04-23 Ta Instr Inc Method and apparatus for modulated differential analysis
US5273632A (en) 1992-11-19 1993-12-28 University Of Utah Research Foundation Methods and apparatus for analysis of chromatographic migration patterns
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5912165A (en) 1993-08-18 1999-06-15 Applied Spectral Imaging Ltd Method for chromosome classification by decorrelaiton statistical analysis and hardware therefore
US5580728A (en) 1994-06-17 1996-12-03 Perlin; Mark W. Method and system for genotyping
US5748491A (en) 1995-12-20 1998-05-05 The Perkin-Elmer Corporation Deconvolution method for the analysis of data resulting from analytical separation processes
DE69739357D1 (de) 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
DE19782089T1 (de) * 1996-10-29 1999-12-23 Univ Nebraska At Lincoln Linco Verfahren zum Nachweisen von Punktmutationen in DNA unter Verwendung von Fluoreszenzenergieübergang
US5985120A (en) 1997-06-12 1999-11-16 University Of Massachusetts Rapid analysis of analyte solutions
US6268131B1 (en) 1997-12-15 2001-07-31 Sequenom, Inc. Mass spectrometric methods for sequencing nucleic acids
US6387621B1 (en) 1999-04-27 2002-05-14 University Of Utah Research Foundation Automated analysis of real-time nucleic acid amplification
US6221600B1 (en) 1999-10-08 2001-04-24 Board Of Regents, The University Of Texas System Combinatorial oligonucleotide PCR: a method for rapid, global expression analysis

Also Published As

Publication number Publication date
DE60213730T2 (de) 2007-08-16
ES2269614T3 (es) 2007-04-01
DE60213730D1 (de) 2006-09-21
CA2400601A1 (en) 2003-02-28
US20030104438A1 (en) 2003-06-05
JP4814479B2 (ja) 2011-11-16
EP1288314A3 (de) 2004-02-25
US7630837B2 (en) 2009-12-08
EP1288314A2 (de) 2003-03-05
EP1288314B1 (de) 2006-08-09
JP2003180378A (ja) 2003-07-02

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