PL3746564T3 - Sposób amplifikacji kwasów nukleinowych - Google Patents

Sposób amplifikacji kwasów nukleinowych

Info

Publication number
PL3746564T3
PL3746564T3 PL19743724.7T PL19743724T PL3746564T3 PL 3746564 T3 PL3746564 T3 PL 3746564T3 PL 19743724 T PL19743724 T PL 19743724T PL 3746564 T3 PL3746564 T3 PL 3746564T3
Authority
PL
Poland
Prior art keywords
amplification
nucleic acids
nucleic
acids
Prior art date
Application number
PL19743724.7T
Other languages
English (en)
Inventor
Charles GAWAD
John Easton
Veronica GONZALEZ-PENA
Original Assignee
St. Jude Children's Research Hospital, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by St. Jude Children's Research Hospital, Inc. filed Critical St. Jude Children's Research Hospital, Inc.
Publication of PL3746564T3 publication Critical patent/PL3746564T3/pl

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/319—Exonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119—Strand displacement amplification [SDA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
PL19743724.7T 2018-01-29 2019-01-28 Sposób amplifikacji kwasów nukleinowych PL3746564T3 (pl)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862623471P 2018-01-29 2018-01-29
PCT/US2019/015452 WO2019148119A1 (en) 2018-01-29 2019-01-28 Method for nucleic acid amplification

Publications (1)

Publication Number Publication Date
PL3746564T3 true PL3746564T3 (pl) 2023-05-22

Family

ID=67396233

Family Applications (2)

Application Number Title Priority Date Filing Date
PL19743724.7T PL3746564T3 (pl) 2018-01-29 2019-01-28 Sposób amplifikacji kwasów nukleinowych
PL22217020.1T PL4183886T3 (pl) 2018-01-29 2019-01-28 Sposób amplifikacji kwasów nukleinowych

Family Applications After (1)

Application Number Title Priority Date Filing Date
PL22217020.1T PL4183886T3 (pl) 2018-01-29 2019-01-28 Sposób amplifikacji kwasów nukleinowych

Country Status (14)

Country Link
US (4) US11905553B2 (pl)
EP (2) EP4183886B1 (pl)
JP (1) JP7457434B2 (pl)
KR (1) KR102653725B1 (pl)
CN (1) CN112041459B (pl)
AU (3) AU2019212953B2 (pl)
CA (1) CA3089756A1 (pl)
DK (2) DK3746564T3 (pl)
EA (1) EA202091673A1 (pl)
ES (2) ES2937927T3 (pl)
IL (3) IL276343B2 (pl)
PL (2) PL3746564T3 (pl)
SG (1) SG11202007225TA (pl)
WO (1) WO2019148119A1 (pl)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL276343B2 (en) 2018-01-29 2024-10-01 St Jude Childrens Res Hospital Inc Method for nucleic acid amplification
US12226746B2 (en) 2019-06-07 2025-02-18 Microsoft Technology Licensing, Llc Reversing bias in polymer synthesis electrode array
CA3149610A1 (en) * 2019-07-31 2021-02-04 BioSkryb Genomics, Inc. Single cell analysis
EP4004230A4 (en) * 2019-07-31 2023-08-09 Bioskryb Genomics, Inc. GENETIC MUTATION ANALYSIS
US11773422B2 (en) 2019-08-16 2023-10-03 Microsoft Technology Licensing, Llc Regulation of polymerase using cofactor oxidation states
US11795450B2 (en) * 2019-09-06 2023-10-24 Microsoft Technology Licensing, Llc Array-based enzymatic oligonucleotide synthesis
CN112831543B (zh) * 2019-11-25 2023-08-29 中国科学院青岛生物能源与过程研究所 单细胞全基因组扩增试剂盒及其应用
EP4103745A4 (en) * 2020-02-10 2024-03-13 Bioskryb Genomics, Inc. Phi29 mutants and use thereof
EP4253559B1 (en) * 2020-02-26 2025-04-02 Illumina, Inc. Kits for genotyping
US20230129100A1 (en) * 2020-05-07 2023-04-27 BioSkryb Genomics, Inc. Detection of low abundance nucleic acids
US11702683B2 (en) 2020-05-28 2023-07-18 Microsoft Technology Licensing, Llc De novo polynucleotide synthesis with substrate-bound polymerase
US12359193B2 (en) 2022-03-04 2025-07-15 Element Biosciences, Inc. Single-stranded splint strands and methods of use
US12371743B2 (en) 2022-03-04 2025-07-29 Element Biosciences, Inc. Double-stranded splint adaptors and methods of use
US12344876B2 (en) 2020-10-30 2025-07-01 Microsoft Technology Licensing, Llc Spatially addressable control of polymerase activity
IL309368A (en) * 2021-06-29 2024-02-01 Agilent Technologies Inc polymerase mutants and use with 3'-OH UNBLOCKED REVERSIBLE TERMINATORS
GB202111194D0 (en) 2021-08-03 2021-09-15 Cergentis B V Method
US20250230493A1 (en) * 2021-12-06 2025-07-17 Cz Biohub Sf, Llc Method for combined genome methylation and variation analyses
CN119585443A (zh) * 2022-05-05 2025-03-07 铂赛基因组学公司 原代模板定向扩增及其方法
IL317477A (en) 2022-07-05 2025-02-01 Element Biosciences Inc PCR-free library preparation using double-stranded probe adapters and methods of use
WO2024049276A1 (ko) * 2022-09-01 2024-03-07 주식회사 키오믹스 다중 표적 dna의 선택적 증폭용 조성물 및 이를 이용한 증폭 방법
IL319170A (en) 2022-09-12 2025-04-01 Element Biosciences Inc Double-stranded probe adapters with universal long probe strands and methods of use
CN117343999B (zh) * 2023-12-05 2024-03-01 上海锐赛循益生物技术有限公司 基于左侧探针退火和右侧探针退火延伸的核酸扩增方法
WO2025235939A1 (en) * 2024-05-10 2025-11-13 Orchid Bioscience, Inc. Methods for balanced amplification of small samples of nucleic acids
WO2026096494A1 (en) 2024-10-29 2026-05-07 Element Biosciences, Inc. Streamlined nucleic acid library preparation and sequencing workflows
WO2026097062A1 (en) 2024-11-04 2026-05-07 Dana-Farber Cancer Institute, Inc. Genomic profiling of cells to monitor minimum residual disease

Family Cites Families (209)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5001050A (en) 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
FR2649518B1 (fr) 1989-07-07 1991-10-18 Bioprobe Systems Sa Procede et dispositif de marquage crypte de haute securite pour la protection d'objets de valeur
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
WO1997014028A2 (en) 1995-10-11 1997-04-17 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
CA2305449A1 (en) 1997-10-10 1999-04-22 President & Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
EP0965044B1 (en) 1997-11-18 2003-03-19 Bio-Rad Laboratories, Inc. Multiplex flow immunoassays with magnetic particles as solid phase
WO1999058724A1 (en) * 1998-05-08 1999-11-18 Life Technologies, Inc. A method for synthesizing a nucleic acid molecule using a ribonuclease
US6150105A (en) 1998-08-20 2000-11-21 Genetic Assays, Inc. Methods of screening nucleic acids for nucleotide variations
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
DE60040603D1 (de) 1999-08-17 2008-12-04 Luminex Corp Verfahren zur analyse einer mehrzahl von proben verschiedenen ursprungs auf einen analyten
DE60027576T2 (de) 1999-08-17 2007-05-03 Luminex Corp., Austin Verkapselung von fluoreszierenden partikeln
CA2384838C (en) * 1999-09-13 2006-07-18 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
WO2002007503A1 (en) 2000-07-25 2002-01-31 The Regents Of The University Of California Electrowetting-driven micropumping
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
EP1423529B1 (en) 2001-01-24 2013-11-20 Genomic Expression APS Assay for analyzing gene expression
GB0102568D0 (en) * 2001-02-01 2001-03-21 Magnetic Biosolutions Sweden A Method
AU2002258997A1 (en) 2001-04-24 2002-11-05 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
CA2453864A1 (en) 2001-06-27 2003-01-09 F. Hoffmann-La Roche Ag A walk-through technique for in vitro recombination of polynucleotide sequences
US6977148B2 (en) 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US7297485B2 (en) 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
EP1451365A4 (en) 2001-11-13 2006-09-13 Rubicon Genomics Inc DNA AMPLIFICATION AND SEQUENCING WITH DNA MOLECULES CREATED BY RANDOM FRAGMENTATION
DE60230918D1 (de) 2001-11-16 2009-03-05 Biogen Idec Inc Polycistronische expression von antikörpern in cho zellen
US7163612B2 (en) 2001-11-26 2007-01-16 Keck Graduate Institute Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like
WO2004034013A2 (en) 2002-05-06 2004-04-22 Colorado State University Research Foundation Genotoxicity analysis
FR2841063B1 (fr) 2002-06-18 2004-09-17 Commissariat Energie Atomique Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
US7413857B2 (en) * 2002-11-21 2008-08-19 Epicentre Technologies Methods for using riboprimers for strand displacement replication of target sequences
US7547380B2 (en) 2003-01-13 2009-06-16 North Carolina State University Droplet transportation devices and methods having a fluid surface
WO2005047696A1 (en) 2003-11-17 2005-05-26 Koninklijke Philips Electronics N.V. System for manipulation of a body of fluid
KR100695123B1 (ko) 2003-12-03 2007-03-14 삼성전자주식회사 Ｔｍ에 따라 기판상에 고정화된 프로브 폴리뉴클레오티드군을 2이상 포함하는 폴리뉴클레오티드 어레이 및 그를이용하는 표적 핵산 검출 방법
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
CN1910440A (zh) 2004-01-14 2007-02-07 卢米尼克斯股份有限公司 用于动态范围扩展的方法和系统
US20060141487A1 (en) 2004-01-26 2006-06-29 Applera Corporation Methods, compositions, and kits for amplifying and sequencing polynucleotides
FR2866493B1 (fr) 2004-02-16 2010-08-20 Commissariat Energie Atomique Dispositif de controle du deplacement d'une goutte entre deux ou plusieurs substrats solides
US7387876B2 (en) 2004-02-27 2008-06-17 President And Fellows Of Harvard College Amplification of trace amounts of nucleic acids
AU2005216549A1 (en) 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
FR2872715B1 (fr) 2004-07-08 2006-11-17 Commissariat Energie Atomique Microreacteur goutte
FR2872809B1 (fr) 2004-07-09 2006-09-15 Commissariat Energie Atomique Methode d'adressage d'electrodes
WO2006073504A2 (en) 2004-08-04 2006-07-13 President And Fellows Of Harvard College Wobble sequencing
JP2006058031A (ja) 2004-08-17 2006-03-02 Hitachi High-Technologies Corp 化学分析装置
CN101650998B (zh) 2005-01-20 2013-02-06 卢米尼克斯股份有限公司 微球、包含其的微球群、以及分析试验样品的方法
US7458661B2 (en) 2005-01-25 2008-12-02 The Regents Of The University Of California Method and apparatus for promoting the complete transfer of liquid drops from a nozzle
CA2594483C (en) 2005-01-28 2014-08-26 Duke University Apparatuses and methods for manipulating droplets on a printed circuit board
US9040237B2 (en) * 2005-03-04 2015-05-26 Intel Corporation Sensor arrays and nucleic acid sequencing applications
US20070023292A1 (en) 2005-07-26 2007-02-01 The Regents Of The University Of California Small object moving on printed circuit board
US7544471B2 (en) 2005-07-30 2009-06-09 Agilent Technologies, Inc. Preparing RNA from a wax-embedded tissue specimen
ATE510930T1 (de) 2005-08-02 2011-06-15 Rubicon Genomics Inc Zusammensetzungen und verfahren zur bearbeitung und amplifikation von dna mit verwendung mehrerer enzyme in einer einzigen reaktion
US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
CA2984772C (en) 2005-09-21 2018-04-03 Luminex Corporation Methods and systems for image data processing
WO2007041201A2 (en) * 2005-10-03 2007-04-12 Applera Corporation Compositions, methods, and kits for amplifying nucleic acids
WO2007120208A2 (en) 2005-11-14 2007-10-25 President And Fellows Of Harvard College Nanogrid rolling circle dna sequencing
CA2633524A1 (en) 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
WO2007103859A2 (en) 2006-03-03 2007-09-13 Luminex Corporation Methods, products, and kits for identifying an analyte in a sample
DE602006018794D1 (de) 2006-04-18 2011-01-20 Advanced Liquid Logic Inc Biochemie auf tröpfchenbasis
US7901947B2 (en) 2006-04-18 2011-03-08 Advanced Liquid Logic, Inc. Droplet-based particle sorting
WO2008007511A1 (en) 2006-07-10 2008-01-17 Hitachi High-Technologies Corporation Liquid transfer device
US9266076B2 (en) 2006-11-02 2016-02-23 The Regents Of The University Of California Method and apparatus for real-time feedback control of electrical manipulation of droplets on chip
AU2007328427A1 (en) 2006-11-06 2008-06-12 Source Precision Medicine, Inc. Gene expression profiling for identification, monitoring and treatment of melanoma
US20080242560A1 (en) 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US7897737B2 (en) * 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
US7893227B2 (en) * 2006-12-05 2011-02-22 Lasergen, Inc. 3′-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing
CN102851369B (zh) 2006-12-13 2015-01-21 卢米耐克斯公司 用于实时pcr多重分析的系统和方法
WO2008089493A2 (en) 2007-01-19 2008-07-24 Fluidigm Corporation High precision microfluidic devices and methods
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US20080269068A1 (en) 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
EP2573562A3 (en) 2007-02-09 2013-10-30 Advanced Liquid Logic, Inc. Droplet actuator devices and methods employing magnetic beads
WO2008116221A1 (en) 2007-03-22 2008-09-25 Advanced Liquid Logic, Inc. Bead sorting on a droplet actuator
US20100130369A1 (en) 2007-04-23 2010-05-27 Advanced Liquid Logic, Inc. Bead-Based Multiplexed Analytical Methods and Instrumentation
US8926811B2 (en) 2007-06-27 2015-01-06 Digital Biosystems Digital microfluidics based apparatus for heat-exchanging chemical processes
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US8685642B2 (en) 2007-07-30 2014-04-01 Agilent Technologies, Inc. Allele-specific copy number measurement using single nucleotide polymorphism and DNA arrays
WO2009032185A2 (en) 2007-08-28 2009-03-12 The Johns Hopkins University Functional assay for indentification of loss-of-function mutations in genes
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
EP2235210B1 (en) 2007-12-21 2015-03-25 President and Fellows of Harvard College Methods for nucleic acid sequencing
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
US8420366B2 (en) 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US20110129827A1 (en) 2008-04-04 2011-06-02 Helicos Biosciences Corporation Methods for transcript analysis
US8093064B2 (en) 2008-05-15 2012-01-10 The Regents Of The University Of California Method for using magnetic particles in droplet microfluidics
EP3002489B1 (en) 2008-05-16 2017-09-20 President and Fellows of Harvard College Valves and other flow control in fluidic systems including microfluidic systems
US20100086501A1 (en) 2008-08-28 2010-04-08 Dermtech International Determining Age Ranges of Skin Samples
EP2210935A1 (en) 2009-01-19 2010-07-28 Deinove Methods for isolating bacteria
JP5909095B2 (ja) 2009-03-13 2016-04-26 プレジデント アンド フェローズ オブ ハーバード カレッジ マイクロ流体デバイスのスケールアップ
US20110319298A1 (en) * 2009-04-21 2011-12-29 Benner Steven A Differential detection of single nucleotide polymorphisms
EP4019977B1 (en) 2009-06-26 2024-11-20 President and Fellows of Harvard College Fluid injection
US20100330556A1 (en) 2009-06-30 2010-12-30 Brian Jon Peter Genome analysis using a nicking endonuclease
US20130053252A1 (en) * 2009-09-25 2013-02-28 President & Fellows Of Harvard College Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides
EP2491123B1 (en) * 2009-10-20 2018-04-18 The Regents of The University of California Single molecule nucleic acid nanoparticles
EP3842150A1 (en) 2009-10-27 2021-06-30 President and Fellows of Harvard College Droplet creation techniques
WO2011055232A2 (en) * 2009-11-04 2011-05-12 Population Genetics Technologies Ltd. Base-by-base mutation screening
CN102639716A (zh) 2009-12-04 2012-08-15 株式会社日立制作所 使用二维cDNA文库的基因表达解析方法
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9493827B2 (en) 2010-01-14 2016-11-15 Deutsches Krebsforschungszentrum Determination of in vivo DNA double-strand break localization and application thereof
EP2539449B9 (en) 2010-02-26 2018-09-05 Qiagen GmbH Process for parallel isolation and/or purification of rna and dna
DK2539472T3 (da) 2010-02-26 2015-08-24 Hennessy Lori Hurtig pcr til str genotypebestemmelse
WO2011150316A1 (en) 2010-05-28 2011-12-01 Sridharan Rajagopalan Obtaining analytes from a tissue specimen
EP2608878A4 (en) 2010-08-23 2017-11-15 President and Fellows of Harvard College Acoustic waves in microfluidics
WO2012071096A2 (en) 2010-09-03 2012-05-31 The Johns Hopkins University Arid1a and ppp2r1a mutations in cancer
EP2625320B1 (en) 2010-10-08 2019-03-27 President and Fellows of Harvard College High-throughput single cell barcoding
US20120214160A1 (en) 2011-01-14 2012-08-23 Life Technologies Corporation Methods, compositions, and kits for detecting rare cells
AU2012236713B2 (en) 2011-03-30 2016-11-24 Bio-Rad Laboratories, Inc. Injection of multiple volumes into or out of droplets
EP2691540B1 (en) 2011-03-31 2016-01-20 GnuBIO, Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
GB201106254D0 (en) * 2011-04-13 2011-05-25 Frisen Jonas Method and product
AU2012242847B2 (en) 2011-04-15 2017-01-19 The Johns Hopkins University Safe sequencing system
CN103917661A (zh) 2011-05-12 2014-07-09 网络百奥有限公司 用于快速多重扩增str基因座的方法和组合物
JP6122843B2 (ja) 2011-05-23 2017-04-26 プレジデント アンド フェローズ オブ ハーバード カレッジ 多重エマルジョンを含むエマルジョンの制御
CN103890191B (zh) 2011-05-27 2018-12-04 哈佛大学校长及研究员协会 单细胞全基因组扩增方法
US9464331B2 (en) 2011-07-06 2016-10-11 Quest Diagnostics Investments Incorporated Direct amplification and detection of viral and bacterial pathogens
US10704164B2 (en) 2011-08-31 2020-07-07 Life Technologies Corporation Methods, systems, computer readable media, and kits for sample identification
CN106423314B (zh) 2011-09-28 2021-03-02 哈佛学院院长等 用于液滴产生和/或流体操纵的系统和方法
SG10201510189WA (en) * 2011-10-19 2016-01-28 Nugen Technologies Inc Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing
JP6103778B2 (ja) 2011-11-03 2017-03-29 キアジェン ゲイサーズバーグ インコーポレイテッド カルボキシル化表面を使用して細胞を固定、単離、および濃縮するための材料および方法
CA2854023A1 (en) 2011-11-07 2013-05-16 Illumina, Inc. Integrated sequencing apparatuses and methods of use
WO2013081864A1 (en) 2011-11-29 2013-06-06 Life Technologies Corporation Methods and compositions for multiplex pcr
WO2013119753A1 (en) 2012-02-08 2013-08-15 President And Fellows Of Harvard College Droplet formation using fluid breakup
US20130309676A1 (en) 2012-05-18 2013-11-21 Alfred E. Mann Foundation For Scientific Research Biased n-mers identification methods, probes and systems for target amplification and detection
US9968901B2 (en) * 2012-05-21 2018-05-15 The Scripps Research Institute Methods of sample preparation
US8895249B2 (en) * 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US10058306B2 (en) 2012-06-22 2018-08-28 Preprogen, LLC Method for obtaining fetal cells and fetal cellular components
GB2539836B (en) 2012-08-13 2017-03-29 Univ California Methods for detecting target nucleic acids in sample lysate droplets
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9422535B2 (en) 2013-04-25 2016-08-23 Thermo Fisher Scientific Baltics Uab phi29 DNA polymerase mutants having increased thermostability and processivity
DK3013983T3 (da) 2013-06-25 2023-03-06 Prognosys Biosciences Inc Spatialt kodede biologiske assays ved brug af en mikrofluidisk anordning
US20150051088A1 (en) * 2013-08-19 2015-02-19 Abbott Molecular Inc. Next-generation sequencing libraries
WO2015031528A1 (en) 2013-08-27 2015-03-05 Gnubio, Inc. Microfluidic devices and methods of their use
US20150099947A1 (en) 2013-10-04 2015-04-09 Access Business Group International Llc Skin youthfulness index, methods and applications thereof
US11339435B2 (en) 2013-10-18 2022-05-24 Molecular Loop Biosciences, Inc. Methods for copy number determination
WO2015131110A1 (en) 2014-02-27 2015-09-03 Igenomx International Genomics Corporation Methods for analysis of somatic mobile elements, and uses thereof
US10975371B2 (en) 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
CN106536709A (zh) 2014-06-16 2017-03-22 基纽拜奥股份有限公司 尺寸交替的注入到液滴中以促进分选
US10480021B2 (en) 2014-06-23 2019-11-19 Yale University Methods for closed chromatin mapping and DNA methylation analysis for single cells
WO2015200717A2 (en) 2014-06-27 2015-12-30 The Regents Of The University Of California Pcr-activated sorting (pas)
CN112941065A (zh) 2014-07-21 2021-06-11 亿明达股份有限公司 使用crispr-cas系统的多核苷酸富集
AU2015315103B2 (en) 2014-09-09 2022-01-27 Igenomx International Genomics Corporation Methods and compositions for rapid nucleic acid library preparation
WO2016065056A1 (en) 2014-10-22 2016-04-28 The Regents Of The University Of California High definition microdroplet printer
CA2967447C (en) 2014-12-05 2025-07-08 Foundation Medicine, Inc. MULTIGEN ANALYSIS OF TUMOR SAMPLES
US11186865B2 (en) 2015-01-21 2021-11-30 Agency For Science, Technology And Research Single cell RNA and mutational analysis PCR (SCRM-PCR): a method for simultaneous analysis of DNA and RNA at the single-cell level
AU2016215304B2 (en) 2015-02-04 2022-01-27 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
EP3792378B1 (en) 2015-02-04 2023-08-23 The University of British Columbia Methods and devices for analyzing particles
US20180080070A1 (en) * 2015-04-08 2018-03-22 Saint Louis University Method for template-dependent multiple displacement amplification
US9771575B2 (en) 2015-06-19 2017-09-26 Agilent Technologies, Inc. Methods for on-array fragmentation and barcoding of DNA samples
WO2017019456A2 (en) 2015-07-27 2017-02-02 Illumina, Inc. Spatial mapping of nucleic acid sequence information
EP3332254A1 (en) 2015-08-07 2018-06-13 POC Medical Systems Inc. Microfluidic devices and methods of use thereof
WO2017088169A1 (en) 2015-11-27 2017-06-01 Coyote Bioscience Co., Ltd. Methods and systems for nucleic acid amplification
WO2017136520A1 (en) 2016-02-04 2017-08-10 President And Fellows Of Harvard College Mitochondrial genome editing and regulation
WO2017176834A2 (en) 2016-04-06 2017-10-12 University Of Florida Research Foundation, Incorporated Measurement of genomic age for predicting the risk of cancer
CN109072234B (zh) 2016-05-13 2022-11-29 豪夫迈·罗氏有限公司 基于蛋白质的样品采集基质和装置
CN109074430B (zh) 2016-05-26 2022-03-29 贝克顿迪金森公司 分子标记计数调整方法
CN109997192A (zh) 2016-06-15 2019-07-09 哈佛学院董事及会员团体 用于基于规则的基因组设计的方法
NL2017177B1 (en) 2016-07-15 2018-01-18 Seatechenergy Ip B V Installation and process for anaerobic digestion of organic material
WO2018027048A1 (en) 2016-08-05 2018-02-08 Bio-Rad Laboratories, Inc. Second strand direct
US11142791B2 (en) 2016-08-10 2021-10-12 The Regents Of The University Of California Combined multiple-displacement amplification and PCR in an emulsion microdroplet
AU2017331459B2 (en) 2016-09-26 2023-04-13 Becton, Dickinson And Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
WO2018075436A1 (en) 2016-10-17 2018-04-26 West Jason A A High resolution spatial genomic analysis of tissues and cell aggregates
WO2018085835A1 (en) 2016-11-07 2018-05-11 Battelle Memorial Institute Nanoscale biochemical sample preparation and analysis
WO2018118997A2 (en) 2016-12-19 2018-06-28 Quantum-Si Incorporated Polymerizing enzymes for sequencing reactions
CN110462053A (zh) 2016-12-21 2019-11-15 加利福尼亚大学董事会 使用基于水凝胶的液滴进行单细胞基因组测序
EP3565907B1 (en) 2017-01-06 2022-05-04 Editas Medicine, Inc. Methods of assessing nuclease cleavage
WO2018165459A1 (en) 2017-03-08 2018-09-13 The University Of Chicago Method for highly sensitive dna methylation analysis
EP3601595A4 (en) 2017-03-21 2020-12-16 Muwells, Inc. SEALED MICROWELL DOSAGE
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN111093501A (zh) 2017-06-02 2020-05-01 西北大学 用于表皮采样和感测的微流体系统
KR102628035B1 (ko) 2017-06-07 2024-01-22 오레곤 헬스 앤드 사이언스 유니버시티 메틸화 서열결정을 위한 단일 세포 전체 게놈 라이브러리
FI3662083T3 (fi) 2017-08-01 2024-11-20 Illumina Inc Geneettisen materiaalin ja kirjastovalmisteen spatiaalinen indeksointi hydrogeelihelmiä ja virtauskennoja käyttämällä
JP6998404B2 (ja) 2017-08-01 2022-02-04 深▲セン▼恒特基因有限公司 標的ヌクレオチド配列の富化及び決定方法
WO2019046644A1 (en) 2017-08-31 2019-03-07 Coopergenomics, Inc. SYSTEMS AND METHODS FOR DETECTION OF NOVO MUTATIONS IN HUMAN EMBRYOS
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US11732257B2 (en) 2017-10-23 2023-08-22 Massachusetts Institute Of Technology Single cell sequencing libraries of genomic transcript regions of interest in proximity to barcodes, and genotyping of said libraries
AU2019205809A1 (en) 2018-01-08 2020-08-13 Cradle Genomics, Inc. Methods and kits for nucleic acid isolation
IL276343B2 (en) 2018-01-29 2024-10-01 St Jude Childrens Res Hospital Inc Method for nucleic acid amplification
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
EP4253562A3 (en) 2018-02-13 2023-10-25 Illumina, Inc. Dna sequencing using hydrogel beads
US12103004B2 (en) 2018-03-12 2024-10-01 Silicon Valley Scientific, Inc. Method and apparatus for processing tissue and other samples encoding cellular spatial position information
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
JP2021526791A (ja) 2018-06-04 2021-10-11 ガーダント ヘルス， インコーポレイテッド セルフリー核酸の細胞起源を決定するための方法およびシステム
CN113166807B (zh) 2018-08-20 2024-06-04 生物辐射实验室股份有限公司 通过分区中条码珠共定位生成核苷酸序列
US20200111573A1 (en) 2018-10-05 2020-04-09 Coopergenomics, Inc. Systems and methods for autonomous machine interpretation of high throughput biological assays for embryo selection
CA3113271A1 (en) 2018-10-26 2020-04-30 Illumina, Inc. Modulating polymer beads for dna processing
EP3973074A4 (en) 2019-05-22 2023-09-06 Mission Bio, Inc. METHOD AND DEVICE FOR SIMULTANEOUS TARGETED SEQUENCING OF DNA, RNA AND PROTEIN
US20220298489A1 (en) 2019-06-17 2022-09-22 Georgia Tech Research Corporation Filtration-based systems and methods for isolation of clustered particles
GB201909325D0 (en) 2019-06-28 2019-08-14 Cs Genetics Ltd Reagents and methods for analysis for microparticles
CA3149610A1 (en) 2019-07-31 2021-02-04 BioSkryb Genomics, Inc. Single cell analysis
EP4004230A4 (en) 2019-07-31 2023-08-09 Bioskryb Genomics, Inc. GENETIC MUTATION ANALYSIS
EP4058597B1 (en) 2019-11-14 2025-10-01 The Trustees of Columbia University in the City of New York Systems, methods, and compositions for generating multi-omic information from single cells
EP4103745A4 (en) 2020-02-10 2024-03-13 Bioskryb Genomics, Inc. Phi29 mutants and use thereof
CN116113714A (zh) 2020-02-18 2023-05-12 生命技术公司 用于检测病毒序列的组合物、试剂盒和方法
EP4118233A1 (en) 2020-03-12 2023-01-18 New England Biolabs, Inc. A rapid diagnostic test for lamp
JP2023518416A (ja) 2020-03-20 2023-05-01 ジェネンテック， インコーポレイテッド 単一細胞の進化を追跡するためのシステム及び方法
CA3178445A1 (en) 2020-03-31 2021-10-07 BioSkryb Genomics, Inc. Detection of low abundance viruses
US20230129100A1 (en) 2020-05-07 2023-04-27 BioSkryb Genomics, Inc. Detection of low abundance nucleic acids
WO2022235764A1 (en) 2021-05-05 2022-11-10 Singular Genomics Systems, Inc. Multiomic analysis device and methods of use thereof
EP4334033A1 (en) 2021-05-07 2024-03-13 BioSkryb Genomics, Inc. High-throughput analysis of biomolecules
EP4367235A1 (en) 2021-07-06 2024-05-15 Singular Genomics Systems, Inc. Compositions and methods for detecting genetic features
US20240271210A1 (en) 2021-07-23 2024-08-15 BioSkryb Genomics, Inc. Spatial nucleic acid analysis
WO2023022975A1 (en) 2021-08-16 2023-02-23 BioSkryb Genomics, Inc. Embryonic nucleic acid analysis
WO2023172923A2 (en) 2022-03-08 2023-09-14 BioSkryb Genomics, Inc. Systems and methods relating to bioinformatics
EP4514961A4 (en) 2022-04-28 2026-05-06 Bioskryb Genomics Inc SINGLE-CELL MULTIOMICS
WO2023212222A1 (en) 2022-04-29 2023-11-02 Qualcomm Incorporated Enhanced measurement object configurations and procedures
CN119585443A (zh) 2022-05-05 2025-03-07 铂赛基因组学公司 原代模板定向扩增及其方法
WO2024026376A2 (en) 2022-07-27 2024-02-01 BioSkryb Genomics, Inc. Methods and systems for multiomic analysis
EP4594728A2 (en) 2022-09-28 2025-08-06 Bioskryb Genomics, Inc. Methods and compositions for fixed sample analysis
WO2024158720A2 (en) 2023-01-23 2024-08-02 BioSkryb Genomics, Inc. Fine needle aspiration methods
WO2025072326A1 (en) 2023-09-26 2025-04-03 BioSkryb Genomics, Inc. Methods, systems, and compositions for cfdna analysis
WO2025085821A1 (en) 2023-10-19 2025-04-24 BioSkryb Genomics, Inc. Methods, systems, and compositions for cell storage and analysis

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