PL396515A1 - Sposób otrzymywania nierozpuszczalnego preparatu lipazy Mucor circinelloides - Google Patents
Sposób otrzymywania nierozpuszczalnego preparatu lipazy Mucor circinelloidesInfo
- Publication number
- PL396515A1 PL396515A1 PL396515A PL39651511A PL396515A1 PL 396515 A1 PL396515 A1 PL 396515A1 PL 396515 A PL396515 A PL 396515A PL 39651511 A PL39651511 A PL 39651511A PL 396515 A1 PL396515 A1 PL 396515A1
- Authority
- PL
- Poland
- Prior art keywords
- selector
- mucor circinelloides
- glycerol tristearate
- oil
- ibpua
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 102000004882 Lipase Human genes 0.000 title abstract 2
- 108090001060 Lipase Proteins 0.000 title abstract 2
- 239000004367 Lipase Substances 0.000 title abstract 2
- 235000019421 lipase Nutrition 0.000 title abstract 2
- 241000235526 Mucor racemosus Species 0.000 title 1
- DCXXMTOCNZCJGO-UHFFFAOYSA-N tristearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCCCCCCCCCC)COC(=O)CCCCCCCCCCCCCCCCC DCXXMTOCNZCJGO-UHFFFAOYSA-N 0.000 abstract 10
- 241000306281 Mucor ambiguus Species 0.000 abstract 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 abstract 4
- 239000007787 solid Substances 0.000 abstract 4
- 229920001817 Agar Polymers 0.000 abstract 3
- 239000008272 agar Substances 0.000 abstract 3
- 235000005687 corn oil Nutrition 0.000 abstract 3
- 239000007788 liquid Substances 0.000 abstract 3
- 235000008390 olive oil Nutrition 0.000 abstract 3
- 239000004006 olive oil Substances 0.000 abstract 3
- 239000008399 tap water Substances 0.000 abstract 3
- 235000020679 tap water Nutrition 0.000 abstract 3
- 239000012153 distilled water Substances 0.000 abstract 2
- 239000006260 foam Substances 0.000 abstract 2
- 239000002054 inoculum Substances 0.000 abstract 2
- 239000002609 medium Substances 0.000 abstract 2
- 239000003208 petroleum Substances 0.000 abstract 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- 239000002028 Biomass Substances 0.000 abstract 1
- 230000003213 activating effect Effects 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 229910052697 platinum Inorganic materials 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
- 150000003626 triacylglycerols Chemical class 0.000 abstract 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Sposób otrzymywania nierozpuszczalnego preparatu lipazy Mucor circinelloides katalizujacego synteze strukturyzowanych triacylogliceroli polega na hodowli selektanta plesni Mucor circinelloides T66/IBPUA 100, którego zarodniki hoduje sie na podlozu stalym zawierajacym agar, brzeczke piwowarska, tristearynian glicerolu. Hodowle inokulum selektanta prowadzi sie w podlozu cieklym zawierajacym oliwe z oliwek lub olej, namok kukurydziany, wode wodociagowa, a nadto tristearynian glicerolu. Hodowle produkcyjna selektanta prowadzi sie na podlozu cieklym zawierajacym oliwe z oliwek lub olej, namok kukurydziany oraz wode wodociagowa, zaszczepionym inokulum, przy mieszaniu zawartosci fermentora za pomoca mieszadla, do którego przymocowuje sie rozlacznie porowaty nosnik wyhodowanego selektanta w postaci prostopadlosciennych platów pianki poliuretanowej, a otrzymane w wyniku hodowli platy pianki z przerosnieta biomasa selektanta Mucor circinelloides T66/IBPUA 100 oddziela sie od cieczy pohodowlanej, przemywa sie woda destylowana, odwadnia schlodzonym acetonem, ekstrahuje eterem naftowym i suszy w temperaturze pokojowej lub przemywa woda destylowana, zamraza do temperatury -45°C, liofilizuje, przemywa acetonem, eterem naftowym i suszy w temperaturze pokojowej. Selektant plesni Mucor circinelloides T66/IBPUA 100 otrzymuje sie ze szczepu plesni Mucor circinelloides T66, który poddaje sie aktywacji na podlozu stalym zawierajacym tristearynian glicerolu, agar, brzeczke piwowarska, a nastepnie kolonie wyrosle na podlozu stalym poddaje sie selekcji w drodze hodowli wstrzasanej na podlozu cieklym zawierajacym tristearynian glicerolu, oliwe z oliwek lub olej, namok kukurydziany, wode wodociagowa, przy czym procedure aktywowania i selekcji plesni Mucor circinelloides powtarza sie 3-krotnie, a otrzymany selektant przechowuje sie na podlozu stalym o skladzie: tristearynian glicerolu, agar, brzeczka piwowarska o stezeniu 8°Be, w temperaturze 4°C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL396515A PL217359B1 (pl) | 2011-10-03 | 2011-10-03 | Sposób otrzymywania nierozpuszczalnego preparatu lipazy Mucor circinelloides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL396515A PL217359B1 (pl) | 2011-10-03 | 2011-10-03 | Sposób otrzymywania nierozpuszczalnego preparatu lipazy Mucor circinelloides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL396515A1 true PL396515A1 (pl) | 2013-04-15 |
| PL217359B1 PL217359B1 (pl) | 2014-07-31 |
Family
ID=48536265
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL396515A PL217359B1 (pl) | 2011-10-03 | 2011-10-03 | Sposób otrzymywania nierozpuszczalnego preparatu lipazy Mucor circinelloides |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL217359B1 (pl) |
-
2011
- 2011-10-03 PL PL396515A patent/PL217359B1/pl unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL217359B1 (pl) | 2014-07-31 |
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