PL396517A1 - Method for preparing an insoluble product of Mucor racemosus lipase - Google Patents
Method for preparing an insoluble product of Mucor racemosus lipaseInfo
- Publication number
- PL396517A1 PL396517A1 PL396517A PL39651711A PL396517A1 PL 396517 A1 PL396517 A1 PL 396517A1 PL 396517 A PL396517 A PL 396517A PL 39651711 A PL39651711 A PL 39651711A PL 396517 A1 PL396517 A1 PL 396517A1
- Authority
- PL
- Poland
- Prior art keywords
- mucor racemosus
- polyethylene glycol
- glycol distearate
- selector
- oil
- Prior art date
Links
- 241000235526 Mucor racemosus Species 0.000 title abstract 7
- 238000000034 method Methods 0.000 title abstract 3
- 102000004882 Lipase Human genes 0.000 title abstract 2
- 108090001060 Lipase Proteins 0.000 title abstract 2
- 239000004367 Lipase Substances 0.000 title abstract 2
- 235000019421 lipase Nutrition 0.000 title abstract 2
- FPVVYTCTZKCSOJ-UHFFFAOYSA-N Ethylene glycol distearate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCCOC(=O)CCCCCCCCCCCCCCCCC FPVVYTCTZKCSOJ-UHFFFAOYSA-N 0.000 abstract 5
- 239000002202 Polyethylene glycol Substances 0.000 abstract 5
- 229940100608 glycol distearate Drugs 0.000 abstract 5
- 229920001223 polyethylene glycol Polymers 0.000 abstract 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 abstract 4
- 239000007788 liquid Substances 0.000 abstract 4
- 239000007787 solid Substances 0.000 abstract 4
- 229920001817 Agar Polymers 0.000 abstract 3
- 239000008272 agar Substances 0.000 abstract 3
- 235000005687 corn oil Nutrition 0.000 abstract 3
- 235000008390 olive oil Nutrition 0.000 abstract 3
- 239000004006 olive oil Substances 0.000 abstract 3
- 239000008399 tap water Substances 0.000 abstract 3
- 235000020679 tap water Nutrition 0.000 abstract 3
- 239000012153 distilled water Substances 0.000 abstract 2
- 239000002054 inoculum Substances 0.000 abstract 2
- 239000003208 petroleum Substances 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- 239000002028 Biomass Substances 0.000 abstract 1
- 229920005830 Polyurethane Foam Polymers 0.000 abstract 1
- 230000003213 activating effect Effects 0.000 abstract 1
- 239000006260 foam Substances 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000011496 polyurethane foam Substances 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Sposób otrzymywania nierozpuszczalnego preparatu lipazy Mucor racemosus katalizujacego hydrolize oligolipidów polega na hodowli selektanta plesni Mucor racemosus A33/PPDGP, którego zarodniki hoduje sie na podlozu stalym zawierajacym agar, brzeczke piwowarska a nadto distearynian glikolu polietylenowego. Hodowle inokulum selektanta prowadzi sie w podlozu cieklym zawierajacym oliwe z oliwek lub olej, namok kukurydziany, wode wodociagowa oraz distearynian glikolu polietylenowego. Hodowle produkcyjna selektanta prowadzi sie na podlozu cieklym zawierajacym oliwe z oliwek lub olej, namok kukurydziany oraz wode wodociagowa, zaszczepionym inokulum przy mieszaniu zawartosci fermentora za pomoca mieszadla, do którego przymocowuje sie rozlacznie porowaty nosnik wyhodowanego selektanta w postaci prostopadlosciennych platów pianki poliuretanowej, a otrzymane w wyniku hodowli platy pianki z przerosnieta biomasa selektanta Mucor racemosus A33/PPDGP oddziela sie od cieczy pohodowlanej, przemywa sie woda destylowana, odwadnia schlodzonym acetonem, ekstrahuje eterem naftowym i suszy w temperaturze pokojowej lub przemywa woda destylowana, zamraza, liofilizuje, przemywa acetonem, eterem naftowym i suszy w temperaturze pokojowej. Selektant plesni Mucor racemosus A33/PPDGP otrzymuje sie ze szczepu plesni Mucor racemosus A33, który poddaje sie aktywacji na podlozu stalym o skladzie: distearynian glikolu polietylenowego, agar, brzeczka piwowarska. Nastepnie kolonie wyrosle na podlozu stalym poddaje sie selekcji w drodze hodowli wstrzasanej na podlozu cieklym o skladzie: distearynian glikolu polietylenowego, oliwa z oliwek lub olej, namok kukurydziany, woda wodociagowa, przy czym procedure aktywowania i selekcji plesni Mucor racemosus powtarza sie 3-krotnie. Otrzymany selektant przechowuje sie na podlozu stalym o skladzie: distearynian glikolu polietylenowego, agar, brzeczka piwowarska, w temperaturze 4°C.The method of obtaining an insoluble Mucor racemosus lipase preparation catalyzing the hydrolysis of oligolipids is based on cultivating the Mucor racemosus A33 / PPDGP mold selector, the spores of which are grown on a solid base containing agar, brewing broth and, moreover, polyethylene glycol distearate. The cultures of the selectant inoculum are run in a liquid medium containing olive oil or oil, corn soak, tap water and polyethylene glycol distearate. The production of the selector is carried out on a liquid base containing olive oil or oil, corn soak and tap water, inoculated with the inoculum while mixing the fermenter with a stirrer, to which the porous carrier of the grown selector in the form of rectangular rectangular plates is attached, and polyurethane foams obtained as a result of growing foam flakes with overexposed biomass of Mucor racemosus A33 / PPDGP, they are separated from the culture liquid, washed with distilled water, dehydrated with cooled acetone, extracted with petroleum ether and dried at room temperature or washed with distilled water, freezing, lyophilizing, washing with acetone, petroleum ether and dried at room temperature. The Mucor racemosus A33 / PPDGP mildew selectant is obtained from the Mucor racemosus A33 mildew strain, which is activated on a solid medium composed of: polyethylene glycol distearate, agar, brewing wort. Then the colonies grew on a solid support are selected by shaking on a liquid support composed of: polyethylene glycol distearate, olive oil or oil, corn soak, tap water, and the procedure for activating and selecting Mucor racemosus is repeated three times. The resulting selector is stored on a solid base composed of: polyethylene glycol distearate, agar, brewing wort, at 4 ° C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL396517A PL217361B1 (en) | 2011-10-03 | 2011-10-03 | Method for preparing an insoluble product of Mucor racemosus lipase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL396517A PL217361B1 (en) | 2011-10-03 | 2011-10-03 | Method for preparing an insoluble product of Mucor racemosus lipase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL396517A1 true PL396517A1 (en) | 2013-04-15 |
| PL217361B1 PL217361B1 (en) | 2014-07-31 |
Family
ID=48536267
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL396517A PL217361B1 (en) | 2011-10-03 | 2011-10-03 | Method for preparing an insoluble product of Mucor racemosus lipase |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL217361B1 (en) |
-
2011
- 2011-10-03 PL PL396517A patent/PL217361B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL217361B1 (en) | 2014-07-31 |
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