PL404594A1 - A method for obtaining an insoluble lipase preparation and a method for producing fatty acid esters in the presence of this preparation as an enzymatic catalyst - Google Patents
A method for obtaining an insoluble lipase preparation and a method for producing fatty acid esters in the presence of this preparation as an enzymatic catalystInfo
- Publication number
- PL404594A1 PL404594A1 PL404594A PL40459413A PL404594A1 PL 404594 A1 PL404594 A1 PL 404594A1 PL 404594 A PL404594 A PL 404594A PL 40459413 A PL40459413 A PL 40459413A PL 404594 A1 PL404594 A1 PL 404594A1
- Authority
- PL
- Poland
- Prior art keywords
- fatty acid
- mucor circinelloides
- rapeseed oil
- waste
- selector
- Prior art date
Links
- 235000014113 dietary fatty acids Nutrition 0.000 title abstract 7
- 239000000194 fatty acid Substances 0.000 title abstract 7
- 229930195729 fatty acid Natural products 0.000 title abstract 7
- 238000000034 method Methods 0.000 title abstract 5
- 102000004882 Lipase Human genes 0.000 title abstract 3
- 108090001060 Lipase Proteins 0.000 title abstract 3
- 239000004367 Lipase Substances 0.000 title abstract 3
- 235000019421 lipase Nutrition 0.000 title abstract 3
- 239000003054 catalyst Substances 0.000 title abstract 2
- -1 fatty acid esters Chemical class 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 230000002255 enzymatic effect Effects 0.000 title 1
- 241000306281 Mucor ambiguus Species 0.000 abstract 6
- 235000019484 Rapeseed oil Nutrition 0.000 abstract 5
- 150000004665 fatty acids Chemical class 0.000 abstract 5
- 238000007670 refining Methods 0.000 abstract 5
- 239000002699 waste material Substances 0.000 abstract 5
- 240000008042 Zea mays Species 0.000 abstract 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 4
- 235000005822 corn Nutrition 0.000 abstract 4
- 229920001817 Agar Polymers 0.000 abstract 3
- 239000008272 agar Substances 0.000 abstract 3
- 239000007788 liquid Substances 0.000 abstract 3
- 239000007787 solid Substances 0.000 abstract 3
- 239000008399 tap water Substances 0.000 abstract 3
- 235000020679 tap water Nutrition 0.000 abstract 3
- 241000238557 Decapoda Species 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 230000003213 activating effect Effects 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000012153 distilled water Substances 0.000 abstract 1
- 239000002054 inoculum Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Wynalazek dotyczy sposobu otrzymywania nierozpuszczalnego preparatu lipazy Mucor circinelloides w drodze hodowli selektanta pleśni Mucor circinelloides TB80300A, którego zarodniki hoduje się na podłożu stałym zawierającym: agar, namok kukurydziany, zemulgowaną frakcję technicznych kwasów tłuszczowych stanowiącą odpad w procesie rafinacji oleju rzepakowego i wodę wodociągową. Hodowlę inokulum selektanta prowadzi się w podłożu ciekłym zawierającym: namok kukurydziany, wodę wodociągową, a nadto frakcję technicznych kwasów tłuszczowych stanowiącą odpad przy rafinacji oleju rzepakowego, zaś hodowlę produkcyjną selektanta prowadzi się na podłożu ciekłym o składzie jak podłoże inokularne. Selektant pleśni Mucor circinelloides TB80300A otrzymuje się ze szczepu pleśni Mucor circinelloides TB wyizolowanego z pancerzy krewetek, który poddaje się aktywacji na podłożu stałym o składzie: zemulgowaną frakcja technicznych kwasów tłuszczowych stanowiąca odpad przy rafinacji oleju rzepakowego, namok kukurydziany, agar, woda destylowana, po czym wyrosłe kolonie poddaje się selekcji w drodze hodowli wstrząsanej na podłożu ciekłym o składzie: frakcja technicznych kwasów tłuszczowych stanowiąca odpad przy rafinacji oleju rzepakowego, namok kukurydziany, woda wodociągowa, przy czym procedurę aktywowania i selekcji pleśni Mucor circinelloides powtarza się kilkakrotnie, a otrzymany selektant przechowuje się na podłożu stałym o składzie: frakcja technicznych kwasów tłuszczowych stanowiąca odpad przy rafinacji oleju rzepakowego, agar, brzeczka piwowarska, w temperaturze 4°C. Wynalazek dotyczy także wytwarzania estrów kwasów tłuszczowych i alkoholi w obecności nierozpuszczalnego preparatu lipazy Mucor circinelloides, otrzymanego opisanym wyżej sposobem, jako katalizatora enzymatycznego.The invention relates to a method for obtaining an insoluble Mucor circinelloides lipase preparation by culturing the Mucor circinelloides TB80300A mold selector, the spores of which are grown on a solid medium containing: agar, corn soak, an emulsified fraction of technical fatty acids constituting waste in the rapeseed oil refining process and tap water. Cultivation of the selector inoculum is carried out in a liquid medium containing: corn soak, tap water, and also a fraction of technical fatty acids constituting waste during rapeseed oil refining, while the cultivation of the selectorant is carried out on a liquid medium with the composition as an inocular medium. The Mucor circinelloides TB80300A mold selector is obtained from the Mucor circinelloides TB mold strain isolated from shrimp armor, which is activated on a solid substrate composed of: the emulsified technical fatty acid fraction that is waste when refining rapeseed oil, corn soak, agar, and distilled water, the grown colonies are selected by shaking culture on a liquid medium composed of: technical fatty acid fraction being the waste when refining rapeseed oil, corn soak, tap water, the procedure of activating and selecting the Mucor circinelloides mold is repeated several times, and the obtained selector is stored on a solid medium composed of: technical fatty acid fraction being waste from rapeseed oil refining, agar, brewing wort, at 4 ° C. The invention also relates to the production of fatty acid esters in the presence of an insoluble Mucor circinelloides lipase preparation obtained by the method described above as an enzyme catalyst.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL404594A PL227592B1 (en) | 2013-07-08 | 2013-07-08 | Method for obtaining indissoluble preparation of lipase and method for production of fatty acid esters in the presence of that preparation as an enzyme catalyst |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL404594A PL227592B1 (en) | 2013-07-08 | 2013-07-08 | Method for obtaining indissoluble preparation of lipase and method for production of fatty acid esters in the presence of that preparation as an enzyme catalyst |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL404594A1 true PL404594A1 (en) | 2015-01-19 |
| PL227592B1 PL227592B1 (en) | 2018-01-31 |
Family
ID=52305497
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL404594A PL227592B1 (en) | 2013-07-08 | 2013-07-08 | Method for obtaining indissoluble preparation of lipase and method for production of fatty acid esters in the presence of that preparation as an enzyme catalyst |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL227592B1 (en) |
-
2013
- 2013-07-08 PL PL404594A patent/PL227592B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL227592B1 (en) | 2018-01-31 |
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