PL404593A1 - A method for obtaining an insoluble lipase preparation and a method for producing fatty acid esters in the presence of this preparation as an enzymatic catalyst - Google Patents
A method for obtaining an insoluble lipase preparation and a method for producing fatty acid esters in the presence of this preparation as an enzymatic catalystInfo
- Publication number
- PL404593A1 PL404593A1 PL404593A PL40459313A PL404593A1 PL 404593 A1 PL404593 A1 PL 404593A1 PL 404593 A PL404593 A PL 404593A PL 40459313 A PL40459313 A PL 40459313A PL 404593 A1 PL404593 A1 PL 404593A1
- Authority
- PL
- Poland
- Prior art keywords
- waste
- mucor circinelloides
- rapeseed oil
- selector
- mold
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 9
- 102000004882 Lipase Human genes 0.000 title abstract 3
- 108090001060 Lipase Proteins 0.000 title abstract 3
- 239000004367 Lipase Substances 0.000 title abstract 3
- 235000019421 lipase Nutrition 0.000 title abstract 3
- 235000014113 dietary fatty acids Nutrition 0.000 title abstract 2
- 239000000194 fatty acid Substances 0.000 title abstract 2
- 229930195729 fatty acid Natural products 0.000 title abstract 2
- -1 fatty acid esters Chemical class 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 239000003054 catalyst Substances 0.000 title 1
- 230000002255 enzymatic effect Effects 0.000 title 1
- 241000306281 Mucor ambiguus Species 0.000 abstract 6
- 235000019484 Rapeseed oil Nutrition 0.000 abstract 5
- 239000003925 fat Substances 0.000 abstract 5
- 238000007670 refining Methods 0.000 abstract 5
- 239000002699 waste material Substances 0.000 abstract 5
- 240000008042 Zea mays Species 0.000 abstract 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 4
- 235000005822 corn Nutrition 0.000 abstract 4
- 229920001817 Agar Polymers 0.000 abstract 3
- 239000008272 agar Substances 0.000 abstract 3
- 239000007788 liquid Substances 0.000 abstract 3
- 239000008399 tap water Substances 0.000 abstract 3
- 235000020679 tap water Nutrition 0.000 abstract 3
- 239000012153 distilled water Substances 0.000 abstract 2
- 239000007787 solid Substances 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- 241000238557 Decapoda Species 0.000 abstract 1
- 230000003213 activating effect Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000008247 solid mixture Substances 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Przedmiotem wynalazku jest sposób otrzymywania nierozpuszczalnego preparatu lipazy Mucor circinelloides w drodze hodowli selektanta pleśni Mucor circinelloides TB80301A, którego zarodniki hoduje się na podłożu stałym zawierającym: agar, namok kukurydziany, zemulgowaną frakcję tłuszczów kanałowych stanowiącą odpad w procesie rafinacji oleju rzepakowego i wodę wodociągową, hodowlę inokulum selektanta prowadzi się w podłożu ciekłym zawierającym namok kukurydziany, wodę wodociągową, frakcję tłuszczów kanałowych stanowiącą odpad w procesie rafinacji oleju rzepakowego, zaś hodowlę produkcyjną selektanta prowadzi się na podłożu ciekłym o składzie jak podłoże inokularne. Selektant pleśni Mucor circinelloides TB80301A otrzymuje się ze szczepu pleśni Mucor circinelloides TB wyizolowanego z pancerzy krewetek, który poddaje się aktywacji na podłożu stałym zawierającym: zemulgowaną frakcję tłuszczów kanałowych stanowiącą odpad w procesie rafinacji oleju rzepakowego, namok kukurydziany, agar, wodę destylowaną, po czym wyrosłe kolonie poddaje się selekcji w drodze hodowli wstrząsanej na podłożu ciekłym zawierającym: frakcję tłuszczów kanałowych stanowiących odpad w procesie rafinacji oleju rzepakowego, namok kukurydziany, wodę wodociągową, przy czym procedurę aktywowania i selekcji pleśni Mucor circinelloides powtarza się kilkakrotnie, a otrzymany selektant przechowuje się na podłożu stałym o składzie: frakcja tłuszczów kanałowych stanowiącej odpad w procesie rafinacji oleju rzepakowego, agar, brzeczka piwowarska w temperaturze 4°C. Wynalazek dotyczy także sposobów wytwarzania estrów kwasów tłuszczowych i alkoholi w obecności nierozpuszczalnego preparatu lipazy Mucor circinelloides.The subject of the invention is a method for obtaining an insoluble Mucor circinelloides lipase preparation by cultivating the Mucor circinelloides TB80301A mold selector, the spores of which are cultured on a solid medium containing: agar, corn soak, emulsified fraction of fats being waste in the process of rapeseed oil refining and tap water the selector is carried out in a liquid medium containing corn soak, tap water, the channel fat fraction being waste in the process of rapeseed oil refining, while the selectorant production culture is carried out on a liquid medium with the composition as an inocular medium. The Mucor circinelloides TB80301A mold selector is obtained from the Mucor circinelloides TB mold strain isolated from shrimp armor, which is activated on a solid substrate containing: the emulsified channel fat fraction being the waste in the refining process of rapeseed oil, corn soak, agar, distilled water, followed by distilled water colonies are selected by shaking culture on a liquid medium containing: a fraction of channel fats being waste in the process of rapeseed oil refining, corn soak, tap water, the procedure of activating and selecting Mucor circinelloides mold is repeated several times and the selected selector is stored on the medium solid composition: channel fat fraction constituting waste in the refining process of rapeseed oil, agar, brewing wort at 4 ° C. The invention also relates to methods for producing fatty acid esters in the presence of an insoluble Mucor circinelloides lipase preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL404593A PL227591B1 (en) | 2013-07-08 | 2013-07-08 | Method for obtaining indissoluble preparation of lipase and method for production of fatty acid esters in the presence of that preparation as an enzyme catalyst |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL404593A PL227591B1 (en) | 2013-07-08 | 2013-07-08 | Method for obtaining indissoluble preparation of lipase and method for production of fatty acid esters in the presence of that preparation as an enzyme catalyst |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL404593A1 true PL404593A1 (en) | 2015-01-19 |
| PL227591B1 PL227591B1 (en) | 2018-01-31 |
Family
ID=52305496
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL404593A PL227591B1 (en) | 2013-07-08 | 2013-07-08 | Method for obtaining indissoluble preparation of lipase and method for production of fatty acid esters in the presence of that preparation as an enzyme catalyst |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL227591B1 (en) |
-
2013
- 2013-07-08 PL PL404593A patent/PL227591B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL227591B1 (en) | 2018-01-31 |
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