PL412848A1 - Method for producing polymer with the promoter sequence TATAAA in artificial cassette TATA and application of this polymer for recognizing and/or selectively determining hexanucleotide sequence of DNA. - Google Patents
Method for producing polymer with the promoter sequence TATAAA in artificial cassette TATA and application of this polymer for recognizing and/or selectively determining hexanucleotide sequence of DNA.Info
- Publication number
- PL412848A1 PL412848A1 PL412848A PL41284815A PL412848A1 PL 412848 A1 PL412848 A1 PL 412848A1 PL 412848 A PL412848 A PL 412848A PL 41284815 A PL41284815 A PL 41284815A PL 412848 A1 PL412848 A1 PL 412848A1
- Authority
- PL
- Poland
- Prior art keywords
- polymer
- dna
- tata
- tataaa
- cassette
- Prior art date
Links
- 229920000642 polymer Polymers 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title 1
- 108020004414 DNA Proteins 0.000 abstract 3
- 229920000344 molecularly imprinted polymer Polymers 0.000 abstract 3
- 102000053602 DNA Human genes 0.000 abstract 2
- 108020004682 Single-Stranded DNA Proteins 0.000 abstract 2
- 150000001413 amino acids Chemical class 0.000 abstract 2
- 239000012491 analyte Substances 0.000 abstract 2
- 238000001514 detection method Methods 0.000 abstract 2
- 238000000157 electrochemical-induced impedance spectroscopy Methods 0.000 abstract 2
- 238000004401 flow injection analysis Methods 0.000 abstract 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 2
- 238000012360 testing method Methods 0.000 abstract 2
- 238000004566 IR spectroscopy Methods 0.000 abstract 1
- 208000026350 Inborn Genetic disease Diseases 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 102000006467 TATA-Box Binding Protein Human genes 0.000 abstract 1
- 108010044281 TATA-Box Binding Protein Proteins 0.000 abstract 1
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 125000000848 adenin-9-yl group Chemical group [H]N([H])C1=C2N=C([H])N(*)C2=NC([H])=N1 0.000 abstract 1
- 210000001124 body fluid Anatomy 0.000 abstract 1
- 239000010839 body fluid Substances 0.000 abstract 1
- 238000001903 differential pulse voltammetry Methods 0.000 abstract 1
- 125000001301 ethoxy group Chemical group [H]C([H])([H])C([H])([H])O* 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 238000005206 flow analysis Methods 0.000 abstract 1
- 208000016361 genetic disease Diseases 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 238000010353 genetic engineering Methods 0.000 abstract 1
- 230000003993 interaction Effects 0.000 abstract 1
- 238000005259 measurement Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000178 monomer Substances 0.000 abstract 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 abstract 1
- 238000006116 polymerization reaction Methods 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 238000011160 research Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Przedmiotem wynalazku jest sposób otrzymywania warstwy polimeru, rozpoznającego aminokwasy wybrane spośród aminokwasów występujących w białku wiążącym TATA, przyłączone przez kasetę TATA, metodą molekularnego wdrukowania prowadzącego do wytworzenia polimeru wdrukowanego molekularnie (MIP), za pomocą polimeryzacji elektrochemicznej w warunkach potencjodynamicznych. We wdrukowaniu tym jako monomery funkcyjne do elektropolimeryzacji stosuje się 4-[2-(6-amino-9H-puryn-9-ylo)etoksy]fenylo-4-[bis(2,2'-bitien-5-ylo)metan] i 1-tyminooctan 4-(di-2,2'-bitiofen-5-ylometylo)fenylu, a jako monomer sieciujący stosuje się (2,4,5,2',4',5'-heksa(tiofen-2-ylo)-3,3'-bitiofen. Wdrukowanie to charakteryzuje się tym, że jako szablon i analit stosuje się sześcionukleotydową sekwencję jednoniciowego DNA lub peptydowy analog jednoniciowego DNA, tj. PNA. Ponadto wynalazek obejmuje zastosowanie warstwy rozpoznającego MIPu, otrzymanej sposobem według wynalazku, jako elementu czujnika chemicznego do wykrywania i/lub selektywnego oznaczania sześcionukleotydowej sekwencji DNA, w płynach ustrojowych pacjenta, z wykorzystaniem impedimetrycznych pomiarów pojemnościowych (CI) w warunkach analizy przepływowo - wstrzykowej (FIA). Ponadto wynalazek obejmuje zastosowanie warstwy rozpoznającej MIPu do badania efektywności wdrukowania a następnie ekstrakcji szablonu TATAAA oraz przyłączania analitu TATAAA przez warstwę MIPu za pomocą elektrochemicznej spektroskopii impedancyjnej (EIS), odbiciowo - absorpcyjnej spektroskopii w podczerwieni (IRRAS) i różnicowej woltamperometrii pulsowej (DPV) oraz zastosowanie warstwy rozpoznającej MIPu do badania oddziaływań z aminokwasami oddziaływującymi z kasetą TATA za pomocą mikrograwimetrii piezoelektrycznej (PM) w warunkach analizy strzykowo - przepływowej (FIA). W szczególności wynalazek ma zastosowanie w inżynierii genetycznej, zwłaszcza do testów genetycznych przy wczesnym wykrywaniu dziedzicznych chorób genetycznych oraz do badań podstawowych modelu transkrypcji DNA.The subject of the invention is a method of obtaining a polymer layer that recognizes amino acids selected from among those found in the TATA binding protein, attached via a TATA cassette, by molecular imprinting to produce a molecularly imprinted polymer (MIP) by electrochemical polymerization under potentiodynamic conditions. In this printing, 4- [2- (6-amino-9H-purin-9-yl) ethoxy] phenyl-4- [bis (2,2'-bitien-5-yl) methane] is used as electropolymerization functional monomers. and 4- (di-2,2'-bitiophen-5-ylmethyl) phenyl 1-thyminoacetate, and (2,4,5,2 ', 4', 5'-hex (thiophen-2- yyl) -3,3'-bitiophene. This print is characterized in that the template and analyte is a six-nucleotide sequence of single-stranded DNA or a single-stranded DNA peptide analog, i.e. as part of a chemical sensor for the detection and / or selective determination of a six-nucleotide DNA sequence in patient's body fluids using impedimetric capacitive measurements (CI) under flow-injection analysis (FIA). In addition, the invention includes the use of a MIPu recognition layer for testing the printing efficiency then template extraction TATAAA and the attachment of the TATAAA analyte through the MIP layer by means of electrochemical impedance spectroscopy (EIS), reflection and absorption infrared spectroscopy (IRRAS) and differential pulse voltammetry (DPV) and the use of the MIPu recognition layer to study interactions with amino acids interacting with the Timetra cassette using micrometry piezoelectric (PM) under conditions of teat-flow analysis (FIA). In particular, the invention is applicable in genetic engineering, in particular for genetic testing for early detection of hereditary genetic diseases and for basic research of the DNA transcription model.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL412848A PL230553B1 (en) | 2014-08-29 | 2015-06-25 | Method for producing polymer with the promoter sequence TATAAA in artificial cassette TATA and application of this polymer for recognizing and/or selectively determining hexanucleotide sequence of DNA. |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PLPL409329 | 2014-08-29 | ||
| PL409329A PL229588B1 (en) | 2014-08-29 | 2014-08-29 | New artificial oligomer with the sequence of nucleobases ATATTT, complementary to the promoter sequence TATAAA, artificial DNA promoter string containing this oligomer and its application for selective detection and determination of TATAAA oligonucleotide |
| PL412848A PL230553B1 (en) | 2014-08-29 | 2015-06-25 | Method for producing polymer with the promoter sequence TATAAA in artificial cassette TATA and application of this polymer for recognizing and/or selectively determining hexanucleotide sequence of DNA. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL412848A1 true PL412848A1 (en) | 2016-03-14 |
| PL230553B1 PL230553B1 (en) | 2018-11-30 |
Family
ID=55450755
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL409329A PL229588B1 (en) | 2014-08-29 | 2014-08-29 | New artificial oligomer with the sequence of nucleobases ATATTT, complementary to the promoter sequence TATAAA, artificial DNA promoter string containing this oligomer and its application for selective detection and determination of TATAAA oligonucleotide |
| PL412848A PL230553B1 (en) | 2014-08-29 | 2015-06-25 | Method for producing polymer with the promoter sequence TATAAA in artificial cassette TATA and application of this polymer for recognizing and/or selectively determining hexanucleotide sequence of DNA. |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL409329A PL229588B1 (en) | 2014-08-29 | 2014-08-29 | New artificial oligomer with the sequence of nucleobases ATATTT, complementary to the promoter sequence TATAAA, artificial DNA promoter string containing this oligomer and its application for selective detection and determination of TATAAA oligonucleotide |
Country Status (1)
| Country | Link |
|---|---|
| PL (2) | PL229588B1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL422867A1 (en) * | 2017-09-18 | 2019-03-25 | Instytut Chemii Fizycznej Polskiej Akademii Nauk | New bis-bithiophene octanucleotide analog with the nucleotide sequence CGCCGCCG, method for obtaining it, an electrochemical sensor that contains this analog, method for producing this sensor, measurement carried out by using it and its application |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL238942B1 (en) * | 2017-11-24 | 2021-10-25 | Inst Chemii Fizycznej Polskiej Akademii Nauk | A synthetic nucleotide polymer with a recognition layer, its preparation and application for the selective determination of a carcinogenic heterocyclic aromatic amine using a chemical sensor |
-
2014
- 2014-08-29 PL PL409329A patent/PL229588B1/en unknown
-
2015
- 2015-06-25 PL PL412848A patent/PL230553B1/en unknown
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL422867A1 (en) * | 2017-09-18 | 2019-03-25 | Instytut Chemii Fizycznej Polskiej Akademii Nauk | New bis-bithiophene octanucleotide analog with the nucleotide sequence CGCCGCCG, method for obtaining it, an electrochemical sensor that contains this analog, method for producing this sensor, measurement carried out by using it and its application |
| PL239699B1 (en) * | 2017-09-18 | 2021-12-27 | Inst Chemii Fizycznej Polskiej Akademii Nauk | A new bisbitiophene analog of an octane nucleotide with the nucleotide sequence CGCCGCCG, a method of its preparation, an electrochemical sensor containing this analog, a method of producing this sensor and the use of an electrochemical sensor |
Also Published As
| Publication number | Publication date |
|---|---|
| PL229588B1 (en) | 2018-08-31 |
| PL230553B1 (en) | 2018-11-30 |
| PL409329A1 (en) | 2016-03-14 |
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