PL422747A1 - Optimized culture medium for neuron cultures, particularly for the primary cortical and thalamic neuron cultures - Google Patents
Optimized culture medium for neuron cultures, particularly for the primary cortical and thalamic neuron culturesInfo
- Publication number
- PL422747A1 PL422747A1 PL422747A PL42274717A PL422747A1 PL 422747 A1 PL422747 A1 PL 422747A1 PL 422747 A PL422747 A PL 422747A PL 42274717 A PL42274717 A PL 42274717A PL 422747 A1 PL422747 A1 PL 422747A1
- Authority
- PL
- Poland
- Prior art keywords
- neuron cultures
- culture medium
- thalamic
- primary cortical
- optimized culture
- Prior art date
Links
- 210000002569 neuron Anatomy 0.000 title abstract 7
- 239000001963 growth medium Substances 0.000 title abstract 3
- 230000001054 cortical effect Effects 0.000 title abstract 2
- 230000000542 thalamic effect Effects 0.000 title abstract 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 239000002609 medium Substances 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Przedmiotem zgłoszenia jest zoptymalizowana pożywka do hodowli neuronów, korzystnie pierwotnych neuronów korowych i wzgórzowych, która zawiera mleczan w stężeniu od około 125 uM do około 6 mM, a glukozę w stężeniu od około 3,0 do około 12,5 mM. Zgłoszenie obejmuje też zastosowanie pożywki do prowadzenia hodowli neuronów i sposób hodowli neuronów wykorzystujący zoptymalizowaną pożywkę do hodowli neuronów.The subject of the application is an optimized medium for growing neurons, preferably primary cortical and thalamic neurons, which contains lactate at a concentration of about 125 µM to about 6 mM and glucose at a concentration of about 3.0 to about 12.5 mM. The application also includes the use of a neuron culture medium and a method for growing neurons using an optimized neuron culture medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL422747A PL235678B1 (en) | 2017-09-05 | 2017-09-05 | Optimized culture medium for neuron cultures, particularly for the primary cortical and thalamic neuron cultures |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL422747A PL235678B1 (en) | 2017-09-05 | 2017-09-05 | Optimized culture medium for neuron cultures, particularly for the primary cortical and thalamic neuron cultures |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL422747A1 true PL422747A1 (en) | 2019-03-11 |
| PL235678B1 PL235678B1 (en) | 2020-10-05 |
Family
ID=65629581
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL422747A PL235678B1 (en) | 2017-09-05 | 2017-09-05 | Optimized culture medium for neuron cultures, particularly for the primary cortical and thalamic neuron cultures |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL235678B1 (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008102118A1 (en) * | 2007-02-19 | 2008-08-28 | Stem Cell Sciences (Uk) Ltd | Large scale production of stem cells |
| CN103484432A (en) * | 2013-09-30 | 2014-01-01 | 栾佐 | Induction medium for inducing neural stem/progenitor cells to be differentiated into oligodendrocyte precursor cells and induction method and application thereof |
| WO2016210313A1 (en) * | 2015-06-24 | 2016-12-29 | Whitehead Institute For Biomedical Research | Culture medium for generating microglia from pluripotent stem cells and related methods |
-
2017
- 2017-09-05 PL PL422747A patent/PL235678B1/en unknown
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008102118A1 (en) * | 2007-02-19 | 2008-08-28 | Stem Cell Sciences (Uk) Ltd | Large scale production of stem cells |
| CN103484432A (en) * | 2013-09-30 | 2014-01-01 | 栾佐 | Induction medium for inducing neural stem/progenitor cells to be differentiated into oligodendrocyte precursor cells and induction method and application thereof |
| WO2016210313A1 (en) * | 2015-06-24 | 2016-12-29 | Whitehead Institute For Biomedical Research | Culture medium for generating microglia from pluripotent stem cells and related methods |
Non-Patent Citations (3)
| Title |
|---|
| A-K. BOUZIER-SORE ET AL, LACTATE IS A PREFERENTIAL OXIDATIVE ENERGY SUBSTRATE OVER GLUCOSE FOR NEURONS IN CULTURE, 2003 * |
| S. WOHNSLAND ET AL, NEURONS AND NEURONAL STEM CELLS SURVIVE IN GLUCOSE-FREE LACTATE AND IN HIGH GLUCOSE CELI CULTURE MEDIUM DURING NORMOXIA AND ANOXIA, 2010 * |
| Z. ALVAREZ ET AL, NEURONAL PROGENITOR MAINTENANCE REQUIRES LACTATE METABOLISM AND PEPCK-M-DIRECTED CATAPLEROSIS, 2016 * |
Also Published As
| Publication number | Publication date |
|---|---|
| PL235678B1 (en) | 2020-10-05 |
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