PL430863A1 - Test paskowy do wykrywania enzootycznej białaczki bydła i sposób wykrywania enzootycznej białaczki bydła z wykorzystaniem tego testu paskowego - Google Patents
Test paskowy do wykrywania enzootycznej białaczki bydła i sposób wykrywania enzootycznej białaczki bydła z wykorzystaniem tego testu paskowegoInfo
- Publication number
- PL430863A1 PL430863A1 PL430863A PL43086319A PL430863A1 PL 430863 A1 PL430863 A1 PL 430863A1 PL 430863 A PL430863 A PL 430863A PL 43086319 A PL43086319 A PL 43086319A PL 430863 A1 PL430863 A1 PL 430863A1
- Authority
- PL
- Poland
- Prior art keywords
- sample
- detection
- strip
- test
- enzootic bovine
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title abstract 3
- 208000003672 enzootic bovine leukosis Diseases 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 235000013336 milk Nutrition 0.000 abstract 2
- 239000008267 milk Substances 0.000 abstract 2
- 210000004080 milk Anatomy 0.000 abstract 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 abstract 1
- 241000283690 Bos taurus Species 0.000 abstract 1
- 239000000020 Nitrocellulose Substances 0.000 abstract 1
- 229920001213 Polysorbate 20 Polymers 0.000 abstract 1
- 101800001271 Surface protein Proteins 0.000 abstract 1
- 239000000427 antigen Substances 0.000 abstract 1
- 102000036639 antigens Human genes 0.000 abstract 1
- 108091007433 antigens Proteins 0.000 abstract 1
- 239000005018 casein Substances 0.000 abstract 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 abstract 1
- 235000021240 caseins Nutrition 0.000 abstract 1
- 235000020247 cow milk Nutrition 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 239000011521 glass Substances 0.000 abstract 1
- 208000032839 leukemia Diseases 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 239000002105 nanoparticle Substances 0.000 abstract 1
- 229920001220 nitrocellulos Polymers 0.000 abstract 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 abstract 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 241000701447 unidentified baculovirus Species 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/20—Detection of antibodies in sample from host which are directed against antigens from microorganisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Przedmiotem wynalazku jest test paskowy do wykrywania enzootycznej białaczki bydła poprzez oznaczenie przeciwciał anty-BLV w próbce charakteryzujący się tym, że zawiera antygen gp51 otrzymany w systemie bakulowirusowym immobilizowany na membranie nitrocelulozowej oraz białka A, G, A/G znakowane barwnymi nanocząsteczkami, a także bibułę szklaną do filtrowania próbki, przy czym analizowaną próbką jest mleko krowie. Kolejnym przedmiotem wynalazku jest sposób wykrywania enzootycznej białaczki bydła z wykorzystaniem testu paskowego według wynalazku, obejmujący następujące etapy: a. próbkę mleka rozcieńcza się trzykrotnie 20 mm buforem Tris-HCl pH 7,5 zawierającym 0,3% kazeinę i 0,15% Tween 20; b. próbkę mleka wkrapla się do okienka na kasetce testowej i inkubuje przez 10 minut; c. odczytuje się wynik testu w okienku testowym, przy czym jedna kreska lub plamka oznacza wynik negatywny a dwie kreski lub plamki oznaczają wynik pozytywny.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL430863A PL239791B1 (pl) | 2019-08-12 | 2019-08-12 | Test paskowy do wykrywania enzootycznej białaczki bydła i sposób wykrywania enzootycznej białaczki bydła z wykorzystaniem tego testu paskowego |
| PCT/IB2020/057467 WO2021028802A1 (en) | 2019-08-12 | 2020-08-07 | A strip test for detecting enzootic bovine leukosis and a method for detecting enzootic bovine leukosis with the use of the strip test |
| EP20775931.7A EP4014044A1 (en) | 2019-08-12 | 2020-08-07 | A strip test for detecting enzootic bovine leukosis and a method for detecting enzootic bovine leukosis with the use of the strip test |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL430863A PL239791B1 (pl) | 2019-08-12 | 2019-08-12 | Test paskowy do wykrywania enzootycznej białaczki bydła i sposób wykrywania enzootycznej białaczki bydła z wykorzystaniem tego testu paskowego |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL430863A1 true PL430863A1 (pl) | 2021-02-22 |
| PL239791B1 PL239791B1 (pl) | 2022-01-10 |
Family
ID=72613942
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL430863A PL239791B1 (pl) | 2019-08-12 | 2019-08-12 | Test paskowy do wykrywania enzootycznej białaczki bydła i sposób wykrywania enzootycznej białaczki bydła z wykorzystaniem tego testu paskowego |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP4014044A1 (pl) |
| PL (1) | PL239791B1 (pl) |
| WO (1) | WO2021028802A1 (pl) |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DD212389A3 (de) * | 1982-05-14 | 1984-08-08 | Univ Berlin Humboldt | Verfahren der gp51-antigenherstellung zur diagnose der rinderleukose |
| BE896752A (fr) * | 1983-05-17 | 1983-09-16 | Region Wallone Representee Pat | Procede et chaine reactionnelle pour la diagnostic de la leucose bovine enzootique. |
| JPH01123152A (ja) * | 1987-11-07 | 1989-05-16 | Mitsui Toatsu Chem Inc | 牛白血病ウイルス由来の診断薬を用いた診断方法 |
| EP1933146B1 (en) * | 2006-12-11 | 2011-08-24 | AraGen Biotechnology Co. Ltd. | Rapid immunochromatographic detection by amplification of the colloidal gold signal |
| EP1933144B1 (en) * | 2006-12-11 | 2011-09-28 | AraGen Biotechnology Co. Ltd. | Rapid immunochromatographic detection by amplification of the colloidal gold signal |
| PL217257B1 (pl) * | 2011-04-29 | 2014-06-30 | Inst Immunologii I Terapii Doświadczalnej Pan | Sposób i zestaw do wykrywania enzootycznej białaczki bydła |
| CN108896765A (zh) * | 2018-07-04 | 2018-11-27 | 山东农业大学 | 一种检测a亚群禽白血病病毒的胶体金试纸条及其制备方法 |
-
2019
- 2019-08-12 PL PL430863A patent/PL239791B1/pl unknown
-
2020
- 2020-08-07 WO PCT/IB2020/057467 patent/WO2021028802A1/en not_active Ceased
- 2020-08-07 EP EP20775931.7A patent/EP4014044A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2021028802A1 (en) | 2021-02-18 |
| PL239791B1 (pl) | 2022-01-10 |
| EP4014044A1 (en) | 2022-06-22 |
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