PL433600A1 - Method of obtaining a strain of lactic acid bacteria and use of the thus obtained strain of lactic acid bacteria - Google Patents
Method of obtaining a strain of lactic acid bacteria and use of the thus obtained strain of lactic acid bacteriaInfo
- Publication number
- PL433600A1 PL433600A1 PL433600A PL43360020A PL433600A1 PL 433600 A1 PL433600 A1 PL 433600A1 PL 433600 A PL433600 A PL 433600A PL 43360020 A PL43360020 A PL 43360020A PL 433600 A1 PL433600 A1 PL 433600A1
- Authority
- PL
- Poland
- Prior art keywords
- strain
- vitamin
- lactic acid
- acid bacteria
- isolation
- Prior art date
Links
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title abstract 10
- 241000894006 Bacteria Species 0.000 title abstract 6
- 235000014655 lactic acid Nutrition 0.000 title abstract 5
- 239000004310 lactic acid Substances 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 2
- QYSXJUFSXHHAJI-YRZJJWOYSA-N vitamin D3 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C\C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-YRZJJWOYSA-N 0.000 abstract 5
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 abstract 4
- 238000002955 isolation Methods 0.000 abstract 4
- 235000005282 vitamin D3 Nutrition 0.000 abstract 4
- 239000011647 vitamin D3 Substances 0.000 abstract 4
- 229940021056 vitamin d3 Drugs 0.000 abstract 4
- 241000186000 Bifidobacterium Species 0.000 abstract 2
- 235000013305 food Nutrition 0.000 abstract 2
- 229940088594 vitamin Drugs 0.000 abstract 2
- 229930003231 vitamin Natural products 0.000 abstract 2
- 235000013343 vitamin Nutrition 0.000 abstract 2
- 239000011782 vitamin Substances 0.000 abstract 2
- 150000003722 vitamin derivatives Chemical class 0.000 abstract 2
- 102000016938 Catalase Human genes 0.000 abstract 1
- 108010053835 Catalase Proteins 0.000 abstract 1
- UCTLRSWJYQTBFZ-UHFFFAOYSA-N Dehydrocholesterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)CCCC(C)C)CCC33)C)C3=CC=C21 UCTLRSWJYQTBFZ-UHFFFAOYSA-N 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 241000192125 Firmicutes Species 0.000 abstract 1
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 abstract 1
- 108090000913 Nitrate Reductases Proteins 0.000 abstract 1
- 239000011149 active material Substances 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 239000002243 precursor Substances 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Przedmiotem wynalazku jest sposób pozyskiwania szczepu bakterii kwasu mlekowego, a także zastosowanie tak pozyskanego szczepu bakterii kwasu mlekowego. Zastosowaniem szczepu bakterii kwasu mlekowego, w szczególności wyizolowanego szczepu Bifidobakterii, jako aktywnego biologicznie materiału naturalnego pochodzenia, jest mikrobiologiczna synteza witaminy D3. Służą do tego najlepiej wyizolowywane takie bakterie gram-dodatnie szczepu, które nie wykazują całkowitej aktywności katalazy oraz reduktazy azotanowej, korzystnie w teście wykonanym roztworem nadtlenku wodoru, przy czym ich kolonie mają kolor biały, a kształt wypukły i owalny. Wyizolowane szczepy inokuluje się najlepiej nie później niż na 24 h od wyizolowania, przy czym inokulację oraz następującą po niej inkubację dającą wzrost i namnażanie witaminy D3 prowadzi się w warunkach przemysłowych, podczas wytwarzania produktu żywnościowego, a samo wyizolowanie szczepów w warunkach laboratoryjnych. Wyizolowanie oraz namnażanie wspomnianych szczepów bakterii prowadzące do wyizolowania i uzyskania witaminy prowadzi się w obecności 7-dehydrocholesterolu, który to prekursor witaminy D3 jest z sukcesem przekształcany enzymami szczepów wyizolowanej bakterii w ową witaminę. Szczep Bifidobakterii posiada zdolność do uzyskania stężenia witaminy D3 w produkcie żywnościowym o wartości w zakresie od 0,05 µg/l do 50 µg/l.The subject of the invention is a method of obtaining a lactic acid bacteria strain, as well as the use of the lactic acid bacteria strain thus obtained. The use of a lactic acid bacteria strain, in particular an isolated strain of Bifidobacteria, as a biologically active material of natural origin is the microbial synthesis of vitamin D3. For this purpose, gram-positive bacteria of the strain are best isolated, which do not show complete catalase and nitrate reductase activity, preferably in a test performed with a hydrogen peroxide solution, their colonies being white, convex and oval in shape. The isolated strains are preferably inoculated no later than 24 hours after isolation, and the inoculation and subsequent incubation that results in the growth and multiplication of vitamin D3 are carried out under industrial conditions, during the production of the food product, and the isolation of the strains in laboratory conditions. The isolation and multiplication of the aforementioned strains of bacteria leading to the isolation and obtaining of the vitamin is carried out in the presence of 7-dehydrocholesterol, which is a precursor of vitamin D3 which is successfully transformed into this vitamin by the enzymes of the isolated bacterial strains. The Bifidobacterium strain has the ability to obtain the concentration of vitamin D3 in the food product in the range from 0.05 µg / l to 50 µg / l.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL433600A PL241590B1 (en) | 2020-04-19 | 2020-04-19 | Method of obtaining a strain of lactic acid bacteria and use of the thus obtained strain of lactic acid bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL433600A PL241590B1 (en) | 2020-04-19 | 2020-04-19 | Method of obtaining a strain of lactic acid bacteria and use of the thus obtained strain of lactic acid bacteria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL433600A1 true PL433600A1 (en) | 2021-10-25 |
| PL241590B1 PL241590B1 (en) | 2022-10-31 |
Family
ID=78572506
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL433600A PL241590B1 (en) | 2020-04-19 | 2020-04-19 | Method of obtaining a strain of lactic acid bacteria and use of the thus obtained strain of lactic acid bacteria |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL241590B1 (en) |
-
2020
- 2020-04-19 PL PL433600A patent/PL241590B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL241590B1 (en) | 2022-10-31 |
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