PL435984A1 - Method of depigmenting a fish research model, especially of the Danio rerio species with the use of isoetarin, and the use of isoetarin for depigmentation of a fish research model, especially of the Danio rerio species - Google Patents
Method of depigmenting a fish research model, especially of the Danio rerio species with the use of isoetarin, and the use of isoetarin for depigmentation of a fish research model, especially of the Danio rerio speciesInfo
- Publication number
- PL435984A1 PL435984A1 PL435984A PL43598420A PL435984A1 PL 435984 A1 PL435984 A1 PL 435984A1 PL 435984 A PL435984 A PL 435984A PL 43598420 A PL43598420 A PL 43598420A PL 435984 A1 PL435984 A1 PL 435984A1
- Authority
- PL
- Poland
- Prior art keywords
- isoetarin
- research model
- fish
- danio rerio
- species
- Prior art date
Links
- 241000251468 Actinopterygii Species 0.000 title abstract 6
- 241000252212 Danio rerio Species 0.000 title abstract 4
- HUYWAWARQUIQLE-UHFFFAOYSA-N Isoetharine Chemical compound CC(C)NC(CC)C(O)C1=CC=C(O)C(O)=C1 HUYWAWARQUIQLE-UHFFFAOYSA-N 0.000 title abstract 4
- 229960001268 isoetarine Drugs 0.000 title abstract 4
- 230000035614 depigmentation Effects 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 239000002609 medium Substances 0.000 abstract 3
- 235000015097 nutrients Nutrition 0.000 abstract 3
- 239000013543 active substance Substances 0.000 abstract 2
- 238000002845 discoloration Methods 0.000 abstract 2
- AFVFQIVMOAPDHO-UHFFFAOYSA-N Methanesulfonic acid Chemical class CS(O)(=O)=O AFVFQIVMOAPDHO-UHFFFAOYSA-N 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
Przedmiotem zgłoszenia jest sposób depigmentacji modelu badawczego ryby zwłaszcza z gatunku danio pręgowanego, polegający na przygotowaniu medium odżywczego dla ryb zawierającego mieszaninę soli w wodzie demineralizowanej z dodatkiem substancji aktywnej, a następnie umieszczeniu w nim modelu badawczego ryby i utrzymywaniu w medium odżywczym do uzyskania pożądanego stopnia odbarwienia, korzystnie całkowitego odbarwienia charakteryzuje się tym, że jako substancję aktywną w medium odżywczym stosuje się izoetarynę w postaci soli kwasu mesylowego, w ilości od 1,7106 g do 5,0313 g na 1L wodnego roztworu medium hodowlanego, odpowiadający stężeniu izoetaryny w zakresie od 5,1 mM do 15 mM. Przedmiotem zgłoszenia jest także izoetaryna do zastosowania w depigmentacji ryby, zwłaszcza z gatunku danio pręgowatego.The subject of the application is a method of depigmenting a fish research model, especially of the zebrafish species, consisting in preparing a nutrient medium for fish containing a mixture of salt in demineralized water with the addition of an active substance, and then placing the fish research model in it and maintaining it in a nutrient medium until the desired degree of discoloration is obtained. , preferably complete discoloration is characterized in that isoetarin in the form of mesylic acid salt is used as the active substance in the nutrient medium in an amount of 1.7106 g to 5.0313 g per 1L of the aqueous solution of the culture medium, corresponding to an isoetarin concentration in the range of 5 , 1 mM to 15 mM. The subject of the application is also isoetharin for use in the depigmentation of fish, especially of the zebrafish species.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL435984A PL241022B1 (en) | 2020-11-16 | 2020-11-16 | Method of depigmenting a fish research model, especially of the Danio rerio species with the use of isoetarin, and the use of isoetarin for depigmentation of a fish research model, especially of the Danio rerio species |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL435984A PL241022B1 (en) | 2020-11-16 | 2020-11-16 | Method of depigmenting a fish research model, especially of the Danio rerio species with the use of isoetarin, and the use of isoetarin for depigmentation of a fish research model, especially of the Danio rerio species |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL435984A1 true PL435984A1 (en) | 2021-11-22 |
| PL241022B1 PL241022B1 (en) | 2022-07-18 |
Family
ID=78719755
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL435984A PL241022B1 (en) | 2020-11-16 | 2020-11-16 | Method of depigmenting a fish research model, especially of the Danio rerio species with the use of isoetarin, and the use of isoetarin for depigmentation of a fish research model, especially of the Danio rerio species |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL241022B1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL449745A1 (en) * | 2024-09-09 | 2026-03-16 | Uniwersytet Medyczny W Lublinie | Method of depigmentation of the Danio rerio fish research model using cefazolin and its complexes with cyclodextrins and a new application of cefazolin and its complexes with cyclodextrins |
-
2020
- 2020-11-16 PL PL435984A patent/PL241022B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL241022B1 (en) | 2022-07-18 |
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