PL449745A1 - Method of depigmentation of the Danio rerio fish research model using cefazolin and its complexes with cyclodextrins and a new application of cefazolin and its complexes with cyclodextrins - Google Patents

Method of depigmentation of the Danio rerio fish research model using cefazolin and its complexes with cyclodextrins and a new application of cefazolin and its complexes with cyclodextrins

Info

Publication number
PL449745A1
PL449745A1 PL449745A PL44974524A PL449745A1 PL 449745 A1 PL449745 A1 PL 449745A1 PL 449745 A PL449745 A PL 449745A PL 44974524 A PL44974524 A PL 44974524A PL 449745 A1 PL449745 A1 PL 449745A1
Authority
PL
Poland
Prior art keywords
cefazolin
cyclodextrins
complexes
depigmentation
nutrient medium
Prior art date
Application number
PL449745A
Other languages
Polish (pl)
Inventor
Anita Płazińska
Wojciech Płaziński
Original Assignee
Uniwersytet Medyczny W Lublinie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniwersytet Medyczny W Lublinie filed Critical Uniwersytet Medyczny W Lublinie
Priority to PL449745A priority Critical patent/PL449745A1/en
Publication of PL449745A1 publication Critical patent/PL449745A1/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/10—Culture of aquatic animals of fish
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Environmental Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Toxicology (AREA)
  • Medicinal Preparation (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Istotą zgłoszenia jest sposób indukowania depigmentacji larw ryb, zwłaszcza danio pręgowanego polegający na przygotowaniu medium odżywczego dla ryb zawierającego mieszaninę soli w wodzie demineralizowanej z dodatkiem substancji aktywnej, a następnie umieszczeniu w nim ryby danio pręgowanego i utrzymywaniu ich w medium odżywczym do pożądanego stopnia odbarwienia, korzystnie całkowitego odbarwienia, gdzie jako substancję aktywną stosuje się cefazolinę w stężeniu od 0,15 mM do 1,4 mM, w ilości od 0,0682 g do 0,6363 g na 1 L wodnego roztworu medium odżywczego. Sposób depigmentacji w alternatywnym rozwiązaniu polega na tym, że jako substancję aktywną stosuje się kompleks cefazoliny z cyklodekstryną o stosunku molowym na etapie formulacji, korzystnie 1:2, odpowiadający stężeniu cefazoliny w finalnym medium w zakresie od 0,11 mM do 0,25 mM, co odpowiada ilości od 0,3888 g do 0,884 g kompleksu 1:2 na 1 L wodnego roztworu medium odżywczego, przy czym stosuje się warunki jak opisane w pierwszym rozwiązaniu. Zgłoszenie pozwala uzyskiwać każdorazowo do celów badawczych transparentną rybę zwłaszcza w badaniach in vivo prowadzonych nad rozwojem narządów i funkcjonowaniem organizmu, patogenezą chorób, testowaniem leków mających na celu opracowanie nowych skutecznych terapii.The essence of the application is a method of inducing depigmentation of fish larvae, especially zebrafish, consisting in preparing a nutrient medium for fish containing a mixture of salts in demineralized water with the addition of an active substance, and then placing zebrafish therein and keeping them in the nutrient medium until the desired degree of discoloration, preferably complete discoloration, wherein cefazolin is used as the active substance at a concentration of 0.15 mM to 1.4 mM, in an amount of 0.0682 g to 0.6363 g per 1 L of an aqueous solution of the nutrient medium. The alternative depigmentation method involves using a cefazolin-cyclodextrin complex as the active ingredient, preferably in a 1:2 molar ratio at the formulation stage, corresponding to a cefazolin concentration in the final medium ranging from 0.11 mM to 0.25 mM, which corresponds to an amount of 0.3888 g to 0.884 g of the 1:2 complex per 1 L of aqueous nutrient solution, using the same conditions as described in the first embodiment. The application allows for obtaining transparent fish for research purposes, particularly in in vivo studies on organ development and body function, disease pathogenesis, and drug testing aimed at developing new effective therapies.

PL449745A 2024-09-09 2024-09-09 Method of depigmentation of the Danio rerio fish research model using cefazolin and its complexes with cyclodextrins and a new application of cefazolin and its complexes with cyclodextrins PL449745A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL449745A PL449745A1 (en) 2024-09-09 2024-09-09 Method of depigmentation of the Danio rerio fish research model using cefazolin and its complexes with cyclodextrins and a new application of cefazolin and its complexes with cyclodextrins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL449745A PL449745A1 (en) 2024-09-09 2024-09-09 Method of depigmentation of the Danio rerio fish research model using cefazolin and its complexes with cyclodextrins and a new application of cefazolin and its complexes with cyclodextrins

Publications (1)

Publication Number Publication Date
PL449745A1 true PL449745A1 (en) 2026-03-16

Family

ID=99061457

Family Applications (1)

Application Number Title Priority Date Filing Date
PL449745A PL449745A1 (en) 2024-09-09 2024-09-09 Method of depigmentation of the Danio rerio fish research model using cefazolin and its complexes with cyclodextrins and a new application of cefazolin and its complexes with cyclodextrins

Country Status (1)

Country Link
PL (1) PL449745A1 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL241022B1 (en) * 2020-11-16 2022-07-18 Univ Medyczny W Lublinie Method of depigmenting a fish research model, especially of the Danio rerio species with the use of isoetarin, and the use of isoetarin for depigmentation of a fish research model, especially of the Danio rerio species

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL241022B1 (en) * 2020-11-16 2022-07-18 Univ Medyczny W Lublinie Method of depigmenting a fish research model, especially of the Danio rerio species with the use of isoetarin, and the use of isoetarin for depigmentation of a fish research model, especially of the Danio rerio species

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHEN B. ET AL.: "Front Pharmacol. 2017 Jun 26; 8: 403", EMBRYO AND DEVELOPMENTAL TOXICITY OF CEFAZOLIN SODIUM IMPURITIES IN ZEBRAFISH. *
KARLSSON J. ET AL.: "Mar Biotechnol 2001 Nov; 3(6): 522-7", GENERATING TRANSPARENT ZEBRAFISH: A REFINED METHOD TO IMPROVE DETECTION OF GENE EXPRESSION DURING EMBRYONIC DEVELOPMENT. *

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