PL437660A1 - Zestaw starterów, skład reagentów oraz metoda wykrywania bakterii Staphylococcus aureus opornej na metycylinę (MRSA) - Google Patents
Zestaw starterów, skład reagentów oraz metoda wykrywania bakterii Staphylococcus aureus opornej na metycylinę (MRSA)Info
- Publication number
- PL437660A1 PL437660A1 PL437660A PL43766021A PL437660A1 PL 437660 A1 PL437660 A1 PL 437660A1 PL 437660 A PL437660 A PL 437660A PL 43766021 A PL43766021 A PL 43766021A PL 437660 A1 PL437660 A1 PL 437660A1
- Authority
- PL
- Poland
- Prior art keywords
- sequence
- mrsa
- reverse
- seq
- staphylococcus aureus
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 title abstract 2
- 241000191967 Staphylococcus aureus Species 0.000 title abstract 2
- 229960003085 meticillin Drugs 0.000 title abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- 238000001514 detection method Methods 0.000 title 1
- 230000000295 complement effect Effects 0.000 abstract 5
- 241000894006 Bacteria Species 0.000 abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 208000015181 infectious disease Diseases 0.000 abstract 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 abstract 1
- 101150008979 mecA gene Proteins 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/119—Strand displacement amplification [SDA]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Przedmiotem zgłoszenia jest zestaw starterów do powielania sekwencji nukleotydowej genu mecA Staphylococcus aureus metycylino—oporny (MRSA), charakteryzujący się tym, że zawiera zestawy starterów wewnętrznych o następujących sekwencjach nukleotydowych a) i b), a także zestawy starterów zewnętrznych zawierający następujące sekwencje nukleotydowe c) i d): a) 5' GAAGGTGTGCTTACAAGTGCTAATA 3'- (sekwencja nukleinowa SEQ ID NO: 3 lub sekwencja do niej odwrotna i komplementarna) - połączona od końca 3', korzystnie mostkiem TTTT, z sekwencją 5' CAACATGAAAAATGATTATGGCTC 3'- (sekwencja nukleinowa SEQ ID NO: 4 lub sekwencja do niej odwrotna i komplementarna) b) 5' TGACGTCTATCCATTTATGTATGGC 3' (sekwencja nukleinowa SEQ ID NO: 5 lub sekwencja do niej odwrotna i komplementarna) - połączona od końca 3' korzystnie mostkiem TTTT, z sekwencją 5' AGGTTCTTTTTTATCTTCGGTTA 3'-(sekwencja nukleinowa SEQ ID NO: 6 lub sekwencja do niej odwrotna i komplementarna) c) 5' TGATGCTAAAGTTCAAAAGAGT 3' sekwencja nukleinowa SEQ ID NO: 1 lub sekwencja do niej odwrotna komplementarna oraz 5' GTAATCTGGAACTTGTTGAGC 3' sekwencja nukleinowa SEQ ID NO: 2 lub sekwencja do niej odwrotna komplementarna. Przedmiotem zgłoszenia jest ponadto sposób wykrywania bakterii MRSA, sposób wykrywania infekcji wywołanej bakterią MRSA oraz zestaw do wykrywania infekcji wywołanej bakterią MRSA.
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL437660A PL437660A1 (pl) | 2021-04-22 | 2021-04-22 | Zestaw starterów, skład reagentów oraz metoda wykrywania bakterii Staphylococcus aureus opornej na metycylinę (MRSA) |
| PCT/PL2022/050025 WO2022225410A1 (en) | 2021-04-22 | 2022-04-21 | Primer set, reagent composition and method for the detection of methicillin-resistant staphylococcus aureus (mrsa) |
| US18/556,185 US20240200152A1 (en) | 2021-04-22 | 2022-04-21 | Primer set, reagent composition and method for the detection of methicillin-resistant staphylococcus aureus (mrsa) |
| JP2023564624A JP2024518750A (ja) | 2021-04-22 | 2022-04-21 | メチシリン耐性黄色ブドウ球菌（ｍｒｓａ）の検出のためのプライマーセット、試薬組成物および方法 |
| EP22792091.5A EP4326908A4 (en) | 2021-04-22 | 2022-04-21 | Primer set, reagent composition and method for the detection of methicillin-resistant Staphylococcus aureus (MRSA) |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL437660A PL437660A1 (pl) | 2021-04-22 | 2021-04-22 | Zestaw starterów, skład reagentów oraz metoda wykrywania bakterii Staphylococcus aureus opornej na metycylinę (MRSA) |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PL437660A1 true PL437660A1 (pl) | 2022-10-24 |
Family
ID=83722422
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL437660A PL437660A1 (pl) | 2021-04-22 | 2021-04-22 | Zestaw starterów, skład reagentów oraz metoda wykrywania bakterii Staphylococcus aureus opornej na metycylinę (MRSA) |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20240200152A1 (pl) |
| EP (1) | EP4326908A4 (pl) |
| JP (1) | JP2024518750A (pl) |
| PL (1) | PL437660A1 (pl) |
| WO (1) | WO2022225410A1 (pl) |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1020534T1 (de) | 1998-11-09 | 2001-03-01 | Eiken Kagaku K.K., Tokio/Tokyo | Verfahren zur synthetisieren von nukleinsäure |
| ATE437225T1 (de) | 2000-09-19 | 2009-08-15 | Eiken Chemical | Verfahren zur polynukleotidsynthese |
| JP2006271370A (ja) | 2005-03-02 | 2006-10-12 | Kitasato Inst:The | 黄色ブドウ球菌とメチシリン耐性菌の検出方法及びキット |
| EP1921156A1 (en) | 2006-11-10 | 2008-05-14 | bioMerieux B.V. | Improved multiplex nucleic acid amplification using blocked primers |
| US9828622B2 (en) | 2008-05-05 | 2017-11-28 | The General Hospital Corporation | Photoactivatable antimicrobial agents and therapeutic and diagnostic methods of using same |
| US9447461B2 (en) | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
| CN105671146A (zh) | 2009-09-25 | 2016-06-15 | 艾利尔圣迭戈公司 | 粗基质中的核酸的检测 |
| US20130266942A1 (en) | 2012-04-06 | 2013-10-10 | Geneohm Sciences Cananda, Inc. | Sequences for detection and identification of methicillin-resistant staphylococcus aureus (mrsa) of mrej type xxi |
| EP2850205B1 (en) | 2012-05-14 | 2019-09-18 | W. Health L.P. | Technique combining pcr and loop-mediated isothermal amplification for the detection of nucleic acids |
| KR101289310B1 (ko) | 2012-11-09 | 2013-07-24 | 주식회사 현일바이오 | Ｍｒｓａ 검출 방법 및 이를 이용한 키트 |
| US20170114393A1 (en) | 2015-06-26 | 2017-04-27 | Great Basin Scientific, Inc. | Specific detection of organisms derived from a sample |
| WO2019032809A1 (en) | 2017-08-11 | 2019-02-14 | Gen-Probe Incorporated | COMPOSITIONS AND METHODS FOR DETECTING STAPHYLOCOCCUS AUREUS |
| KR101908869B1 (ko) | 2017-12-26 | 2018-10-16 | (주)바이오액츠 | 마그네슘 이온의 검출을 통하여 메티실린 내성 황색포도상구균(mrsa)의 감염을 진단하기 위한 키트 |
| KR102126429B1 (ko) | 2019-04-11 | 2020-07-08 | 충북대학교 산학협력단 | 메티실린 내성의 황색포도알균 검출을 위한 프라이머 세트 및 이를 이용한 메티실린 내성 황색포도알균 검출 방법 |
| CN110106267A (zh) * | 2019-05-28 | 2019-08-09 | 上海健康医学院 | 耐甲氧西林金黄色葡萄球菌mecA基因检测用引物组 |
-
2021
- 2021-04-22 PL PL437660A patent/PL437660A1/pl unknown
-
2022
- 2022-04-21 US US18/556,185 patent/US20240200152A1/en active Pending
- 2022-04-21 WO PCT/PL2022/050025 patent/WO2022225410A1/en not_active Ceased
- 2022-04-21 EP EP22792091.5A patent/EP4326908A4/en active Pending
- 2022-04-21 JP JP2023564624A patent/JP2024518750A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2024518750A (ja) | 2024-05-02 |
| EP4326908A1 (en) | 2024-02-28 |
| EP4326908A4 (en) | 2025-04-30 |
| WO2022225410A1 (en) | 2022-10-27 |
| US20240200152A1 (en) | 2024-06-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Liu et al. | Frequency of antiseptic resistance genes and reduced susceptibility to biocides in carbapenem-resistant Acinetobacter baumannii | |
| Ubukata et al. | Identification of penicillin and other beta-lactam resistance in Streptococcus pneumoniae by polymerase chain reaction | |
| Hirotaki et al. | Rapid and accurate identification of human-associated staphylococci by use of multiplex PCR | |
| ES2536887T5 (es) | Métodos y secuencias para la detección e identificación de cepas de Staphylococcus aureus MREJ tipo v resistentes a meticilina | |
| Lim et al. | Loop‐mediated isothermal amplification assay for the rapid detection of Staphylococcus aureus | |
| Sun et al. | Broad-spectrum adaptive antibiotic resistance associated with Pseudomonas aeruginosa mucin-dependent surfing motility | |
| Serray et al. | Genes encoding adhesion factors and biofilm formation in methicillin-resistant Staphylococcus aureus in Morocco | |
| Carvalhais et al. | Controlled RNA contamination and degradation and its impact on qPCR gene expression in S. epidermidis biofilms | |
| Eftekhar et al. | Biofilm formation and detection of icaAB genes in clinical isolates of methicillin resistant Staphylococcus aureus | |
| Zmantar et al. | Multiplex PCR detection of the antibiotic resistance genes in Staphylococcus aureus strains isolated from auricular infections | |
| Behrooz et al. | Study of MazEF, sam, and phd-doc putative toxin–antitoxin systems in Staphylococcus epidermidis | |
| ES2426289T3 (es) | Detección de Staphylococcus aureus e identificación de Staphylococcus aureus resistente a meticilina | |
| Adesoji et al. | Genotypic characterization of aminoglycoside resistance genes from bacteria isolates in selected municipal drinking water distribution sources in southwestern Nigeria | |
| Moukhaiber et al. | Prevalence of Clostridium difficile toxinotypes in infected patients at a tertiary care center in Lebanon | |
| PL437660A1 (pl) | Zestaw starterów, skład reagentów oraz metoda wykrywania bakterii Staphylococcus aureus opornej na metycylinę (MRSA) | |
| Martín et al. | Screening of virulence genes in Staphylococcus aureus isolates from rabbits | |
| Seong et al. | Comparison of complete rpoB gene sequence typing and multi-locus sequence typing for phylogenetic analysis of Staphylococcus aureus | |
| FI20022064A0 (fi) | Nukleiinihappokoettimia, universaaleja alukkeita ja menetelmiä, joissa niitä käytetään | |
| AlSaadi et al. | Study the prevalence of mecA gene in methicillin resistance staphylococcus aureus (MRSA) isolated from different clinical specimens and their antibiotic resistance profile | |
| KR101951846B1 (ko) | 콜리스틴(Colistin) 내성세균을 검출하기 위한 프라이머 및 이를 이용한 콜리스틴 내성세균의 검출방법 | |
| PL435634A1 (pl) | Zestaw starterów, skład reagentów oraz metoda wykrywania bakterii atypowych | |
| PL436263A1 (pl) | Zestaw starterów, skład reagentów oraz metoda wykrywania bakterii atypowych | |
| Ibrahim et al. | Expression Levels of Efflux Pump mexR and norA Genes in Multi-Drug Resistant in Some Bacteria by Using Quantitative RT-PCR Under Stress of Effect Efflux Pump Inhibitors | |
| Amer Hatem et al. | Virulence gene expression of Pseudomonas aeruginosa isolated from children with nephrotic syndrome | |
| Raheema | Rapid methods for detection of methicillin-resistant Staphylococcus aureus in Wasit Province, Iraq. |