ATE394410T1 - Hochspezifische primer, amplifizierungsverfahren und kits - Google Patents

Hochspezifische primer, amplifizierungsverfahren und kits

Info

Publication number
ATE394410T1
ATE394410T1 AT00928752T AT00928752T ATE394410T1 AT E394410 T1 ATE394410 T1 AT E394410T1 AT 00928752 T AT00928752 T AT 00928752T AT 00928752 T AT00928752 T AT 00928752T AT E394410 T1 ATE394410 T1 AT E394410T1
Authority
AT
Austria
Prior art keywords
primers
kits
specific primers
highly specific
amplification processes
Prior art date
Application number
AT00928752T
Other languages
English (en)
Inventor
Sanjay Tyagi
Fred Kramer
Robert Vartikian
Original Assignee
Univ New Jersey Med
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ New Jersey Med filed Critical Univ New Jersey Med
Application granted granted Critical
Publication of ATE394410T1 publication Critical patent/ATE394410T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Adhesives Or Adhesive Processes (AREA)
  • Dental Preparations (AREA)
  • Paints Or Removers (AREA)
AT00928752T 1999-05-24 2000-05-03 Hochspezifische primer, amplifizierungsverfahren und kits ATE394410T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/317,350 US6277607B1 (en) 1999-05-24 1999-05-24 High specificity primers, amplification methods and kits

Publications (1)

Publication Number Publication Date
ATE394410T1 true ATE394410T1 (de) 2008-05-15

Family

ID=23233265

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00928752T ATE394410T1 (de) 1999-05-24 2000-05-03 Hochspezifische primer, amplifizierungsverfahren und kits

Country Status (8)

Country Link
US (2) US6277607B1 (de)
EP (1) EP1185546B1 (de)
JP (1) JP3843215B2 (de)
AT (1) ATE394410T1 (de)
AU (1) AU769219B2 (de)
CA (1) CA2375027C (de)
DE (1) DE60038796D1 (de)
WO (1) WO2000071562A1 (de)

Families Citing this family (137)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
WO2000061498A2 (en) 1999-04-13 2000-10-19 Semitool, Inc. System for electrochemically processing a workpiece
US6893868B2 (en) 1997-02-20 2005-05-17 Onco Immunin, Inc. Homo-doubly labeled compositions for the detection of enzyme activity in biological samples
US6037137A (en) 1997-02-20 2000-03-14 Oncoimmunin, Inc. Fluorogenic peptides for the detection of protease activity
US7312302B2 (en) 1997-02-20 2007-12-25 Oncolmmunin, Inc. Compositions for the detection of enzyme activity in biological samples and methods of use thereof
TWI223678B (en) * 1998-03-20 2004-11-11 Semitool Inc Process for applying a metal structure to a workpiece, the treated workpiece and a solution for electroplating copper
US6565729B2 (en) 1998-03-20 2003-05-20 Semitool, Inc. Method for electrochemically depositing metal on a semiconductor workpiece
US6497801B1 (en) * 1998-07-10 2002-12-24 Semitool Inc Electroplating apparatus with segmented anode array
US20040106110A1 (en) * 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
US20100130368A1 (en) * 1998-07-30 2010-05-27 Shankar Balasubramanian Method and system for sequencing polynucleotides
US7264698B2 (en) 1999-04-13 2007-09-04 Semitool, Inc. Apparatus and methods for electrochemical processing of microelectronic workpieces
US7160421B2 (en) * 1999-04-13 2007-01-09 Semitool, Inc. Turning electrodes used in a reactor for electrochemically processing a microelectronic workpiece
US7351314B2 (en) 2003-12-05 2008-04-01 Semitool, Inc. Chambers, systems, and methods for electrochemically processing microfeature workpieces
US6368475B1 (en) * 2000-03-21 2002-04-09 Semitool, Inc. Apparatus for electrochemically processing a microelectronic workpiece
US6916412B2 (en) 1999-04-13 2005-07-12 Semitool, Inc. Adaptable electrochemical processing chamber
US7438788B2 (en) 1999-04-13 2008-10-21 Semitool, Inc. Apparatus and methods for electrochemical processing of microelectronic workpieces
US7020537B2 (en) 1999-04-13 2006-03-28 Semitool, Inc. Tuning electrodes used in a reactor for electrochemically processing a microelectronic workpiece
US7585398B2 (en) 1999-04-13 2009-09-08 Semitool, Inc. Chambers, systems, and methods for electrochemically processing microfeature workpieces
US7351315B2 (en) 2003-12-05 2008-04-01 Semitool, Inc. Chambers, systems, and methods for electrochemically processing microfeature workpieces
US7189318B2 (en) * 1999-04-13 2007-03-13 Semitool, Inc. Tuning electrodes used in a reactor for electrochemically processing a microelectronic workpiece
US7537886B1 (en) 1999-06-22 2009-05-26 Life Technologies Corporation Primers and methods for the detection and discrimination of nucleic acids
US6692915B1 (en) * 1999-07-22 2004-02-17 Girish N. Nallur Sequencing a polynucleotide on a generic chip
JP4721603B2 (ja) * 1999-11-08 2011-07-13 栄研化学株式会社 変異および/または多型の検出方法
WO2001034838A1 (en) * 1999-11-08 2001-05-17 Eiken Kagaku Kabushiki Kaisha Method of detecting variation or polymorphism
US6664081B2 (en) * 1999-12-17 2003-12-16 Gen-Probe Incorporated Nucleic acid amplification and detection of mycobacterium species
US7205129B1 (en) 2000-02-28 2007-04-17 Qiagen Gmbh Method for reducing artifacts in nucleic acid amplification
EP1188841B1 (de) * 2000-03-31 2008-12-24 Sanko Junyaku Co., Ltd. Sonde zur herstellung einer polymersonde, verfahren zur herstellung einer polymersonde sowie verwendung derselben
CN100422323C (zh) * 2000-04-07 2008-10-01 荣研化学株式会社 使用双链核酸为模板扩增核酸的方法
US7659054B1 (en) * 2000-05-23 2010-02-09 Nuvelo, Inc. Methods for genetic analysis of DNA to detect sequence variances
US6531283B1 (en) 2000-06-20 2003-03-11 Molecular Staging, Inc. Protein expression profiling
US6323009B1 (en) * 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
US6596490B2 (en) * 2000-07-14 2003-07-22 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
US6380377B1 (en) 2000-07-14 2002-04-30 Applied Gene Technologies, Inc. Nucleic acid hairpin probes and uses thereof
EP3034627B1 (de) 2000-10-06 2019-01-30 The Trustees of Columbia University in the City of New York Massives paralleles verfahren zum entschlüsseln von dna und rna
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
CN1348096A (zh) * 2000-10-10 2002-05-08 栾国彦 一种均相特异性检测核酸的探针及应用方法
WO2002061133A2 (en) * 2000-11-09 2002-08-08 Yale University Nucleic acid detection using structured probes
EP1366191A2 (de) * 2000-12-11 2003-12-03 Alexion Pharmaceuticals, Inc. 'nested' haarnadelförmige oligonukleotide zur amplifizierung von nukleinsäure
GB0101397D0 (en) * 2001-01-19 2001-03-07 Amersham Pharm Biotech Uk Ltd Suppression of non-specific nucleic acid amplication
US8507662B2 (en) 2001-01-19 2013-08-13 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
US20020102557A1 (en) * 2001-01-31 2002-08-01 Gentile-Davey Maria C. Self -complementary molecular probe
WO2002063049A2 (en) * 2001-02-02 2002-08-15 Genome Therapeutics Corporation Methods for determining a nucleotide at a specific location within a nucleic acid molecule
US6573051B2 (en) * 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
WO2002083839A2 (en) * 2001-03-30 2002-10-24 Amersham Biosciences Ab P450 single nucleotide polymorphism biochip analysis
US7067849B2 (en) * 2001-07-17 2006-06-27 Lg Electronics Inc. Diode having high brightness and method thereof
AU2002343330A1 (en) 2001-08-31 2003-03-10 Semitool, Inc. Apparatus and methods for electrochemical processing of microelectronic workpieces
US6977148B2 (en) 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US7297485B2 (en) 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US20030165859A1 (en) * 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US7393636B2 (en) * 2001-11-08 2008-07-01 Sanko Junyaku Co., Ltd. Method for forming self-assembly substance using oligonucleotide synthesized by gene amplification reaction, self-assembly substance and method for detecting gene
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
US7553619B2 (en) * 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
US7291459B2 (en) * 2002-12-10 2007-11-06 University Of Alabama At Huntsville Nucleic acid detector and method of detecting targets within a sample
US20040121338A1 (en) * 2002-12-19 2004-06-24 Alsmadi Osama A. Real-time detection of rolling circle amplification products
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
WO2004061134A1 (en) * 2002-12-27 2004-07-22 University Of Medicine And Dentistry Of New Jersey Method for single nucleotide polymorphism detection
US6977153B2 (en) 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
CA2511874A1 (en) * 2003-01-02 2004-07-22 University Of Rochester Hybridization-based biosensor containing hairpin probes and use thereof
WO2004068112A2 (en) * 2003-01-28 2004-08-12 Gorilla Genomics, Inc. Hairpin primer amplification
CA2516299A1 (en) * 2003-02-18 2004-09-02 Applera Corporation Compositions and methods for multiplex analysis of polynucleotides
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
CN1496413A (zh) * 2003-05-09 2004-05-12 清华大学 用于多重pcr条件优化的方法和组分
US20040248103A1 (en) * 2003-06-04 2004-12-09 Feaver William John Proximity-mediated rolling circle amplification
DE10338308B4 (de) 2003-08-15 2006-10-19 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in DNA
EP1718771A4 (de) * 2004-01-02 2008-02-13 Univ Rochester Verfahren zur identifizierung von hairpin-dna-sonden durch partielle faltungsanalyse
WO2006020933A2 (en) * 2004-08-13 2006-02-23 Stratagene California Dual labeled fluorescent probes
WO2006044994A2 (en) * 2004-10-18 2006-04-27 Brandeis University Primers, probes and methods for nucleic acid amplification
US20060194222A1 (en) * 2004-10-20 2006-08-31 Stratagene California Triplex probe compositions and methods for polynucleotide detection
US20060105348A1 (en) * 2004-11-15 2006-05-18 Lee Jun E Compositions and methods for the detection and discrimination of nucleic acids
US20060275792A1 (en) * 2004-11-15 2006-12-07 Lee Jun E Enhancement of nucleic acid amplification using double-stranded DNA binding proteins
EP1863908B1 (de) 2005-04-01 2010-11-17 Qiagen GmbH Reverse transkription und amplifikation von rna bei simultaner degradierung von dna
US20070213293A1 (en) * 2005-04-08 2007-09-13 Nastech Pharmaceutical Company Inc. Rnai therapeutic for respiratory virus infection
CA2603842A1 (en) 2005-04-08 2006-10-19 Nastech Pharmaceutical Company Inc. Rnai therapeutic for respiratory virus infection
ATE547537T1 (de) 2005-04-15 2012-03-15 Epigenomics Ag Verfahren zur bestimmung der dna-methylierung in blut- oder urinproben
US20070015180A1 (en) * 2005-05-02 2007-01-18 Stratagene California Oligonucleotide probe/primer compositions and methods for polynucleotide detection
EP2405024A1 (de) * 2005-07-15 2012-01-11 Life Technologies Corporation Heissstart reverse Transkription durch primer design
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
CN101292045A (zh) * 2005-10-20 2008-10-22 株式会社日立制作所 核酸分析方法
US8017360B2 (en) * 2005-11-10 2011-09-13 Panomics, Inc. Detection of nucleic acids through amplification of surrogate nucleic acids
US20070243534A1 (en) * 2006-04-12 2007-10-18 Michael Seul Probe density considerations and elongation of self-complementary looped probes where probes are attached to a solid phase
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
EP2345740B1 (de) * 2006-06-06 2015-09-30 Gen-Probe Incorporated Markierte Oligonukleotide und ihre Verwendung in Nukleinsäureverstärkungsverfahren
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
JP5286261B2 (ja) 2006-07-17 2013-09-11 ブランディーズ・ユニバーシティ 特殊化オリゴヌクレオチドならびに核酸の増幅および検出でのその使用
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
WO2008021446A2 (en) * 2006-08-15 2008-02-21 Genetag Technology, Inc. Probe-antiprobe compositions and methods for dna or rna detection
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
EP3095873B1 (de) 2006-12-21 2018-04-18 Gen-Probe Incorporated Verfahren und zusammensetzungen zur amplifizierung von nukleinsäuren
DK3101141T3 (da) 2007-06-08 2020-02-03 Epigenomics Ag Fremgangsmåde til methyleringsanalyse
US7811764B2 (en) * 2007-08-14 2010-10-12 University Of Rochester Hybridization-based biosensor containing hairpin probes and use thereof
EP2209911B1 (de) 2007-10-19 2013-10-16 The Trustees of Columbia University in the City of New York Dna-sequenzierung mit reversiblen nicht-fluoreszenz-nukleotid-terminatoren und mit einer spaltbaren markierung modifizierten nukleotidterminatoren und eine deoxyinosineverbindung mit einer reversiblen terminatorgruppe
EP2207900B1 (de) 2007-10-19 2015-04-29 The Trustees of Columbia University in the City of New York Konstruktion und synthese spaltbarer fluoreszenznukleotide als reversible terminatoren für die dna-sequenzierung mittels synthese
US8957002B2 (en) 2007-11-05 2015-02-17 University Of Rochester DNA microarray having hairpin probes tethered to nanostructured metal surface
US20090197254A1 (en) * 2007-12-14 2009-08-06 Ming-Chou Lee Variant scorpion primers for nucleic acid amplification and detection
KR100987352B1 (ko) * 2008-04-15 2010-10-12 주식회사 인트론바이오테크놀로지 비특이 증폭을 감소시킬 수 있는 pcr용 프라이머 및이를 이용한 pcr 방법
US9034605B2 (en) 2009-03-12 2015-05-19 Brandeis University Reagents and methods for PCR
PT2412718T (pt) * 2009-03-26 2016-12-30 Xiamen Amoy Diagnostics Co Ltd Iniciador em forma de anel utilizado na amplificação de ácido nucleico e a sua utilização
CA2766391C (en) 2009-07-01 2021-04-20 Gen-Probe Incorporated Methods and compositions for multiplex nucleic acid amplification
EP2272978B1 (de) * 2009-07-10 2012-04-18 Biotrin International Limited Hochsensibles schnelles isothermisches Verfahren zur Detektion von Punktmutationen und SNPs
US8815515B1 (en) 2009-09-24 2014-08-26 University Of Utah Research Foundation Methods, compositions, and kits for rare allele detection
WO2011059686A2 (en) 2009-10-29 2011-05-19 Wisconsin Alumni Research Foundation Detection of b-cell activating factor as a biomaker for antibody mediated rejection in transplant recipients
US9845492B2 (en) * 2009-12-21 2017-12-19 Seegene, Inc. TSG primer target detection
EP2534263B1 (de) 2010-02-09 2020-08-05 Unitaq Bio Verfahren und zusammensetzungen zur universellen erkennung von nukleinsäuren
EP3135775B1 (de) * 2010-11-10 2018-06-27 Brandeis University Kit zur detektion und identifizierung von mykobakterien
US9169514B2 (en) 2010-12-03 2015-10-27 Brandeis University Detecting nucleic acid variations within populations of genomes
CN106190806B (zh) 2011-04-15 2018-11-06 贝克顿·迪金森公司 扫描实时微流体热循环仪和用于同步的热循环和扫描光学检测的方法
WO2013067202A1 (en) 2011-11-04 2013-05-10 Handylab, Inc. Polynucleotide sample preparation device
US10472683B2 (en) * 2012-06-01 2019-11-12 Akron Biotechnology, LLC. Detection of Mycoplasma in cell cultures and cell culture derived biologicals
JP6544861B2 (ja) 2013-02-07 2019-07-17 ラトガース,ザ ステート ユニバーシティ オブ ニュー ジャージー 高い選択性の核酸増幅プライマー
JP2016512041A (ja) * 2013-03-15 2016-04-25 アボツト・モレキユラー・インコーポレイテツド 多重対立遺伝子の検出
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US20170044525A1 (en) * 2014-04-29 2017-02-16 Illumina, Inc. Multiplexed single cell gene expression analysis using template switch and tagmentation
US10975371B2 (en) * 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
GB201416459D0 (en) 2014-09-17 2014-10-29 Atlas Genetics Ltd Detection method
ES2896198T3 (es) 2014-10-10 2022-02-24 Univ Rutgers Cebadores y sondas de reacción en cadena de polimerasa para Mycobacterium tuberculosis
KR101742681B1 (ko) 2015-01-30 2017-06-01 에스디 바이오센서 주식회사 상보적 염기서열 내지는 미스-매치된 염기를 포함하는 상보적인 염기서열과 연결된 pcr 프라이머 및 이를 이용한 핵산 증폭 방법
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
JP6681804B2 (ja) * 2016-03-30 2020-04-15 大研医器株式会社 変異プライマーの設計方法
CN106282353B (zh) * 2016-08-26 2019-12-10 上海翼和应用生物技术有限公司 一种利用发夹引物进行多重pcr的方法
WO2018102162A1 (en) * 2016-11-30 2018-06-07 Exosome Diagnostics, Inc. Methods and compositions to detect mutations in plasma using exosomal rna and cell free dna from non-small cell lung cancer patients
CN108517354A (zh) * 2017-02-28 2018-09-11 上海茂槿生物技术有限公司 用于多重pcr的引物组合物
EP3937780A4 (de) 2019-03-14 2022-12-07 InSilixa, Inc. Verfahren und systeme zur zeitgesteuerten fluoreszenzbasierten detektion
CN109988758A (zh) * 2019-04-16 2019-07-09 上海快灵生物科技有限公司 一种参与聚合延伸的寡核苷酸链探针及其核酸扩增试剂盒
JP7744025B2 (ja) 2019-10-02 2025-09-25 ラトガース,ザ ステート ユニバーシティ オブ ニュー ジャージー 希少配列変異体を検出するためのアッセイ方法およびキット
CN111826421B (zh) * 2020-07-09 2021-09-21 广州迈景基因医学科技有限公司 一种pcr随机引物和使用其构建靶向测序文库的方法
WO2022072731A1 (en) * 2020-09-30 2022-04-07 Ascella Biosystems, Inc. Rapid and highly sensitive luminescent biomolecule detection
CN116179655A (zh) * 2021-11-29 2023-05-30 北京迅识科技有限公司 一种实时荧光定量检测探针
CN113862339B (zh) * 2021-12-01 2022-03-22 广州滴纳生物科技有限公司 核酸组合产品、检测试剂盒和扩增靶核酸的方法
EP4665870A1 (de) 2023-02-16 2025-12-24 Asecud SA Unterscheidung von nukleinsäuresequenzen mit hohem nukleinsäurengehalt mittels eines vielfachfacettierten nukleinsäuremoleküls
EP4488384A1 (de) 2023-07-05 2025-01-08 Infiniplex Ltd. Primer zur selektiven amplifikation seltener zielsequenzen

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5503979A (en) 1984-05-25 1996-04-02 The Trustees Of Columbia University In The City Of New York Method of using replicatable hybridzable recombinant RNA probes
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
EP0601889A2 (de) * 1992-12-10 1994-06-15 Maine Medical Center Research Institute Nukleinsäure-Sonden
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6037130A (en) 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits

Also Published As

Publication number Publication date
AU769219B2 (en) 2004-01-22
DE60038796D1 (de) 2008-06-19
JP2003500038A (ja) 2003-01-07
AU4693900A (en) 2000-12-12
WO2000071562A1 (en) 2000-11-30
US6277607B1 (en) 2001-08-21
CA2375027C (en) 2010-07-27
CA2375027A1 (en) 2000-11-30
EP1185546A4 (de) 2002-08-21
JP3843215B2 (ja) 2006-11-08
US6365729B1 (en) 2002-04-02
EP1185546A1 (de) 2002-03-13
EP1185546B1 (de) 2008-05-07

Similar Documents

Publication Publication Date Title
DE60038796D1 (de) Hochspezifische primer, amplifizierungsverfahren und kits
BR0014182A (pt) Processos e composições para amplificação isotérmica linear de sequências polinucleotìdicas
WO1997018334A3 (en) Nucleic acid probes complementary to human papillomavirus nucleic acid and related methods and kits
WO2002052031A3 (en) Nucleic acid amplification
WO2002018641A3 (en) Detection of cyp3a4 and cyp2c9 polymorphisms
JP2003524416A5 (de)
WO2004074503A3 (en) Nucleic acid sequencing methods, kits and reagents
NO981239L (no) Modifiserte primere
EP1845160A4 (de) Nukleotidsequenz-amplifikationsverfahren
WO2006026388A3 (en) Single-primer nucleic acid amplification methods
ES2108748T3 (es) Amplificacion de acidos nucleicos con actividad de rna polimerasa dependiente de dna, de rna replicasas.
EP2366803A3 (de) Zusammensetzungen und Verfahren zum Nacheis des West Nil Viruses.
WO2004022770A3 (en) Compositions and methods for synthesizing nucleic acids
EP1212449A4 (de) Verfahren zur amplifikation von signal-flankierenden sequenzen aus unbekannten, genomischen daten
RU98100921A (ru) Праймеры для обнаружения вич-1
ATE469243T1 (de) Dopplezweck -primer und -proben für verbesserte hybridisierungsassays durch zerstörung von sekundärstrukturen
WO1996006949A3 (en) Nucleic acid hybridization assay probes targeted to mycoplasma pneumoniae nucleic acid
DK1026261T3 (da) Forögelse af specificiteten af nukleinsyreamplifikation med bærernukleinsyre
PT1171634E (pt) Pares de iniciadores de amplificacao e de sequenciacao e sua utilizacao
EP3040423A3 (de) Mikro-rna und verwendung davon
DE69616102D1 (de) Amplikationsprimern und Nukleinsäuresonden zum Nachweis von Coccidioides immites
BR0316224A (pt) Sequência de dna da região genÈmica tcd de photorhabdus luminescens
ES2530716T3 (es) Especies de pestivirus
CY1109285T1 (el) Ανακτηση toy dna
EP1193313A4 (de) Verfahren zur subtypenbestimmung von hiv-1

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties