PL437909A1 - Polimeraza Taq-NeqSSB, sposób jej otrzymywania, plazmid rekombinantowy, startery oraz zastosowanie polimerazy - Google Patents

Polimeraza Taq-NeqSSB, sposób jej otrzymywania, plazmid rekombinantowy, startery oraz zastosowanie polimerazy

Info

Publication number
PL437909A1
PL437909A1 PL437909A PL43790921A PL437909A1 PL 437909 A1 PL437909 A1 PL 437909A1 PL 437909 A PL437909 A PL 437909A PL 43790921 A PL43790921 A PL 43790921A PL 437909 A1 PL437909 A1 PL 437909A1
Authority
PL
Poland
Prior art keywords
polymerase
neqssb
primers
recombinant plasmid
taq
Prior art date
Application number
PL437909A
Other languages
English (en)
Other versions
PL243940B1 (pl
Inventor
Marta SKWARECKA
Grzegorz ZIELIŃSKI
Kasjan SZEMIAKO
Dawid NIDZWORSKI
Sabina ŻOŁĘDOWSKA
Original Assignee
Instytut Biotechnologii I Medycyny Molekularnej
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instytut Biotechnologii I Medycyny Molekularnej filed Critical Instytut Biotechnologii I Medycyny Molekularnej
Priority to PL437909A priority Critical patent/PL243940B1/pl
Priority to PCT/PL2022/000031 priority patent/WO2022245230A1/en
Priority to EP22736399.1A priority patent/EP4341389A1/en
Priority to US18/562,014 priority patent/US20240240163A1/en
Publication of PL437909A1 publication Critical patent/PL437909A1/pl
Publication of PL243940B1 publication Critical patent/PL243940B1/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem wynalazku jest polimeraza TaqPol-NeqSSB oraz sposób jej klonowania. Ponadto przedmiotem wynalazku jest wyizolowany plazmid rekombinantowy, startery oraz zastosowanie polimerazy do powielania specyficznych sekwencji wirusa SARS CoV-2.
PL437909A 2021-05-19 2021-05-19 Polimeraza Taq-NeqSSB, sposób jej otrzymywania, plazmid rekombinantowy, startery oraz zastosowanie polimerazy PL243940B1 (pl)

Priority Applications (4)

Application Number Priority Date Filing Date Title
PL437909A PL243940B1 (pl) 2021-05-19 2021-05-19 Polimeraza Taq-NeqSSB, sposób jej otrzymywania, plazmid rekombinantowy, startery oraz zastosowanie polimerazy
PCT/PL2022/000031 WO2022245230A1 (en) 2021-05-19 2022-05-18 Taq-neqssb polymerase, the method of its obtaining, recombinant plasmid, primers, and application of the polymerase.
EP22736399.1A EP4341389A1 (en) 2021-05-19 2022-05-18 Taq-neqssb polymerase, the method of its obtaining, recombinant plasmid, primers, and application of the polymerase
US18/562,014 US20240240163A1 (en) 2021-05-19 2022-05-18 Taq-neqssb polymerase, the method of its obtaining, recombinant plasmid, primers, and application of the polymerase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL437909A PL243940B1 (pl) 2021-05-19 2021-05-19 Polimeraza Taq-NeqSSB, sposób jej otrzymywania, plazmid rekombinantowy, startery oraz zastosowanie polimerazy

Publications (2)

Publication Number Publication Date
PL437909A1 true PL437909A1 (pl) 2022-11-21
PL243940B1 PL243940B1 (pl) 2023-11-06

Family

ID=82361283

Family Applications (1)

Application Number Title Priority Date Filing Date
PL437909A PL243940B1 (pl) 2021-05-19 2021-05-19 Polimeraza Taq-NeqSSB, sposób jej otrzymywania, plazmid rekombinantowy, startery oraz zastosowanie polimerazy

Country Status (4)

Country Link
US (1) US20240240163A1 (pl)
EP (1) EP4341389A1 (pl)
PL (1) PL243940B1 (pl)
WO (1) WO2022245230A1 (pl)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL447278A1 (pl) * 2023-12-23 2025-06-30 Instytut Biotechnologii I Medycyny Molekularnej Zastosowanie polimerazy TaqNeqSSB do normalizacji temperatury przyłączania sond i starterów w reakcji wykrywania jednonukleotydowych mutacji SNP

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070059713A1 (en) * 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
PL426093A1 (pl) * 2018-06-27 2020-01-02 Instytut Biotechnologii I Medycyny Molekularnej Fuzyjna polimeraza kwasu jednołańcuchowego DNA Bst, cząsteczka kwasu nukleinowego kodująca fuzyjną polimerazę DNA NeqSSB-Bst, sposób jej otrzymywania oraz zastosowanie
CN113597468B (zh) * 2019-03-13 2024-09-13 武汉爱博泰克生物科技有限公司 用于快速扩增的突变型Taq聚合酶

Also Published As

Publication number Publication date
US20240240163A1 (en) 2024-07-18
WO2022245230A9 (en) 2024-02-15
PL243940B1 (pl) 2023-11-06
EP4341389A1 (en) 2024-03-27
WO2022245230A1 (en) 2022-11-24

Similar Documents

Publication Publication Date Title
AR130699A2 (es) Inhibidores de la transcriptasa inversa de nucleósidos sustituidos en 4’
EA202092808A1 (ru) Вакцины на основе наночастиц с новыми структурными компонентами
PE20221111A1 (es) Vacunas para vhb y metodos de tratamiento de vhb
MX2018004916A (es) Vacuna contra el virus de la influenza de amplio espectro.
EA202092521A1 (ru) Способы и композиции для индукции защитного иммунитета против инфекции вирусом иммунодефицита человека
BR112019024813A2 (pt) adenovírus recombinantes portando transgenes
CO2017007105A2 (es) Nuevos virus de tilapia
PL431073A1 (pl) Zestaw starterów do wykrywania wirusa brodawczaka ludzkiego typ 16 (HPV16 Human papillomavirus type 16) oraz wirusa brodawczaka ludzkiego typ 18 (HPV18 Human papillomavirus type 18), sposób wykrywania infekcji HPV16 oraz HPV18, zastosowanie zestawu starterów do wykrywania infekcji HPV16 oraz HPV18
MX2020008125A (es) Composiciones que comprenden berberina.
JP2019509717A5 (pl)
PH12015502666B1 (en) Dengue virus vaccine compositions and methods of use thereof
CU24454B1 (es) Composición farmacéutica que comprende los antígenos de la superficie y de la nucleocápsida del virus de la hepatitis b
PL436784A1 (pl) Polimeraza Pwo-NeqSSB, sposób jej otrzymywania, plazmid rekombinantowy, startery oraz zastosowanie polimerazy
MY192543A (en) Dengue virus vaccine compositions and methods of use thereof
EP4282960A3 (en) Modified lipase and use thereof
WO2022245230A9 (en) Taq-neqssb polymerase, the method of its obtaining, recombinant plasmid, primers, and application of the polymerase.
ATE420658T1 (de) Imidazoquinolinamine als adjuvantien für hiv dna vakzine
WO2021019235A3 (en) Hantavirus antigenic composition
MX2021011303A (es) Nueva linea celular que comprende marcador de seleccion y usos de la misma para la produccion de proteinas.
EP4282986A3 (en) Compositions and methods for detecting or quantifying parainfluenza virus
WO2019133666A8 (en) Triazolophthalazine compounds, use as anti-human immunodeficiency virus inhibitors of hiv vif-dependent degradation of apobec3
WO2023064280A3 (en) Compositions comprising hiv envelopes to induce hiv-1 antibodies
EA202190306A1 (ru) Комбинации и способы, включающие ингибитор сборки капсида
TR201900841U5 (tr) Ci̇nsel pozi̇syonlar ve ci̇nsel persormansi arttirmaya yöneli̇k oluşturulmuş tantra koltuğu
IN2013DE01960A (pl)