PL426093A1 - Fuzyjna polimeraza kwasu jednołańcuchowego DNA Bst, cząsteczka kwasu nukleinowego kodująca fuzyjną polimerazę DNA NeqSSB-Bst, sposób jej otrzymywania oraz zastosowanie - Google Patents

Fuzyjna polimeraza kwasu jednołańcuchowego DNA Bst, cząsteczka kwasu nukleinowego kodująca fuzyjną polimerazę DNA NeqSSB-Bst, sposób jej otrzymywania oraz zastosowanie

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PL426093A1
PL426093A1 PL426093A PL42609318A PL426093A1 PL 426093 A1 PL426093 A1 PL 426093A1 PL 426093 A PL426093 A PL 426093A PL 42609318 A PL42609318 A PL 42609318A PL 426093 A1 PL426093 A1 PL 426093A1
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Prior art keywords
bst
polymerase
dna
phusion polymerase
neqssb
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PL426093A
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English (en)
Inventor
Marta ŚPIBIDA
Kasjan Szemianko
Marcin Olszewski
Dawid Midzworski
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Instytut Biotechnologii I Medycyny Molekularnej
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Priority to PL426093A priority Critical patent/PL426093A1/pl
Priority to EP19743078.8A priority patent/EP3814368A1/en
Priority to JP2020571711A priority patent/JP7579557B2/ja
Priority to US17/253,445 priority patent/US20210254034A1/en
Priority to CN201980017798.1A priority patent/CN111819188A/zh
Priority to PCT/PL2019/000046 priority patent/WO2020005084A1/en
Publication of PL426093A1 publication Critical patent/PL426093A1/pl
Priority to IL279262A priority patent/IL279262A/en

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    • C12N9/1241—Nucleotidyltransferases (2.7.7)
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase

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  • Enzymes And Modification Thereof (AREA)
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Abstract

Przedmiotem zgłoszenia jest fuzyjna polimeraza kwasu jednołańcuchowego DNA Bst połączona z białkiem NeqSSB na N-końcu polimerazy za pomocą 6-aminokwasowego linkera o sekwencji aminokwasowej Gly-Ser-Gly-Gly-Val-Asp, gdzie wspomniana polimeraza występuje w trzech różnych odmianach oraz sposób jej otrzymywania. Ponadto przedmiotem zgłoszenia jest cząsteczka kwasu nukleinowego kodująca fuzyjną polimerazę DNA NeqSSB-Bst Full Length, Large Fragment, Short Fragment oraz zastosowanie in vitro fuzyjnej polimerazy kwasu jednołańcuchowego DNA Bst.
PL426093A 2018-06-27 2018-06-27 Fuzyjna polimeraza kwasu jednołańcuchowego DNA Bst, cząsteczka kwasu nukleinowego kodująca fuzyjną polimerazę DNA NeqSSB-Bst, sposób jej otrzymywania oraz zastosowanie PL426093A1 (pl)

Priority Applications (7)

Application Number Priority Date Filing Date Title
PL426093A PL426093A1 (pl) 2018-06-27 2018-06-27 Fuzyjna polimeraza kwasu jednołańcuchowego DNA Bst, cząsteczka kwasu nukleinowego kodująca fuzyjną polimerazę DNA NeqSSB-Bst, sposób jej otrzymywania oraz zastosowanie
EP19743078.8A EP3814368A1 (en) 2018-06-27 2019-06-26 Fusion single-stranded dna polymerase bst, nucleic acid molecule encoding fusion dna polymerase neqssb-bst, method of preparation and utilisation thereof
JP2020571711A JP7579557B2 (ja) 2018-06-27 2019-06-26 融合一本鎖ＤＮＡポリメラーゼＢｓｔ、融合ＤＮＡポリメラーゼＮｅｑＳＳＢ－Ｂｓｔをコードする核酸分子、その調製方法およびその利用
US17/253,445 US20210254034A1 (en) 2018-06-27 2019-06-26 Fusion single-stranded dna polymerase bst, nucleic acid molecule encoding fusion dna polymerase neqssb-bst, method of preparation and utilisation thereof
CN201980017798.1A CN111819188A (zh) 2018-06-27 2019-06-26 融合单链DNA聚合酶Bst、编码融合DNA聚合酶NeqSSB-Bst的核酸分子、其制备方法和用途
PCT/PL2019/000046 WO2020005084A1 (en) 2018-06-27 2019-06-26 Fusion single-stranded dna polymerase bst, nucleic acid molecule encoding fusion dna polymerase neqssb-bst, method of preparation and utilisation thereof
IL279262A IL279262A (en) 2018-06-27 2020-12-07 Fusion of single-stranded DNA polymerase BST, a molecule of nucleic acid that codes for the fusion of DNA polymerase NEQSSB-BST, a method for their preparation and use

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL426093A PL426093A1 (pl) 2018-06-27 2018-06-27 Fuzyjna polimeraza kwasu jednołańcuchowego DNA Bst, cząsteczka kwasu nukleinowego kodująca fuzyjną polimerazę DNA NeqSSB-Bst, sposób jej otrzymywania oraz zastosowanie

Publications (1)

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PL426093A1 true PL426093A1 (pl) 2020-01-02

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PL426093A PL426093A1 (pl) 2018-06-27 2018-06-27 Fuzyjna polimeraza kwasu jednołańcuchowego DNA Bst, cząsteczka kwasu nukleinowego kodująca fuzyjną polimerazę DNA NeqSSB-Bst, sposób jej otrzymywania oraz zastosowanie

Country Status (7)

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US (1) US20210254034A1 (pl)
EP (1) EP3814368A1 (pl)
JP (1) JP7579557B2 (pl)
CN (1) CN111819188A (pl)
IL (1) IL279262A (pl)
PL (1) PL426093A1 (pl)
WO (1) WO2020005084A1 (pl)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102009005289B4 (de) 2009-01-20 2023-06-22 Merck Patent Gmbh Materialien für organische Elektrolumineszenzvorrichtungen, Verfahren zu deren Herstellung und elektronische Vorrichtungen, enthaltend diese
PL241065B1 (pl) * 2020-06-26 2022-08-01 Geneme Spolka Z Ograniczona Odpowiedzialnoscia Zastosowanie fuzyjnej polimerazy DNA Bst-Nec do izotermalnego powielania specyficznych sekwencji wirusa SARS CoV-2
PL241698B1 (pl) * 2021-01-27 2022-11-21 Inst Biotechnologii I Medycyny Molekularnej Polimeraza Pwo-NeqSSB, sposób jej otrzymywania, plazmid rekombinantowy, startery oraz zastosowanie polimerazy
CN112899255B (zh) * 2021-03-06 2022-02-25 苏州瀚源新酶生物科技有限公司 Dna聚合酶及其应用、重组载体及其制备方法和应用、重组工程菌及其应用
PL243940B1 (pl) * 2021-05-19 2023-11-06 Inst Biotechnologii I Medycyny Molekularnej Polimeraza Taq-NeqSSB, sposób jej otrzymywania, plazmid rekombinantowy, startery oraz zastosowanie polimerazy
CN115094047B (zh) * 2022-06-24 2023-06-20 华南理工大学 一种直扩型Bst DNA聚合酶及其制备方法与应用
CN115058404A (zh) * 2022-07-25 2022-09-16 通用生物(南京)有限公司 一种新型的dna合成连接酶
CN115701839A (zh) * 2023-01-05 2023-02-14 深圳无微华斯生物科技有限公司 一种恒温扩增试剂盒及常温保存方法
CN119320460B (zh) * 2024-12-17 2025-03-14 中国农业科学院生物技术研究所 Bst DNA聚合酶和铁蛋白的融合蛋白及其突变体和应用

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SG86311A1 (en) * 1997-04-10 2002-02-19 Univ Singapore Bacillus stearothermophilus dna polymerase i (klenow) clones with reduced 3'-to-5' exonuclease activity
WO2007050125A2 (en) * 2005-05-27 2007-05-03 William Marsh Rice University High processivity polymerases
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
WO2010091203A2 (en) * 2009-02-04 2010-08-12 Lucigen Corporation Rna-and dna-copying enzymes
US20130260422A1 (en) * 2011-09-01 2013-10-03 New England Biolabs, Inc. Compositions and Methods Relating to Variant DNA Polymerases and Synthetic DNA Polymerases
US9963687B2 (en) * 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same

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US20210254034A1 (en) 2021-08-19
JP2021528968A (ja) 2021-10-28
WO2020005084A1 (en) 2020-01-02
CN111819188A (zh) 2020-10-23
IL279262A (en) 2021-01-31
JP7579557B2 (ja) 2024-11-08
EP3814368A1 (en) 2021-05-05

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