PL439149A1 - Method of producing modified bacterial cellulose with antimicrobial properties - Google Patents
Method of producing modified bacterial cellulose with antimicrobial properties Download PDFInfo
- Publication number
- PL439149A1 PL439149A1 PL439149A PL43914921A PL439149A1 PL 439149 A1 PL439149 A1 PL 439149A1 PL 439149 A PL439149 A PL 439149A PL 43914921 A PL43914921 A PL 43914921A PL 439149 A1 PL439149 A1 PL 439149A1
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- Poland
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- minutes
- inoculum
- days
- producing modified
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- 238000000034 method Methods 0.000 title abstract 3
- 229920002749 Bacterial cellulose Polymers 0.000 title 1
- 230000000845 anti-microbial effect Effects 0.000 title 1
- 239000005016 bacterial cellulose Substances 0.000 title 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 abstract 3
- 230000001580 bacterial effect Effects 0.000 abstract 2
- 239000002054 inoculum Substances 0.000 abstract 2
- 239000007788 liquid Substances 0.000 abstract 2
- 238000002156 mixing Methods 0.000 abstract 2
- 241000894006 Bacteria Species 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- 241001474750 Komagataeibacter Species 0.000 abstract 1
- 241001136169 Komagataeibacter xylinus Species 0.000 abstract 1
- 239000001888 Peptone Substances 0.000 abstract 1
- 108010080698 Peptones Proteins 0.000 abstract 1
- 229940041514 candida albicans extract Drugs 0.000 abstract 1
- 239000012153 distilled water Substances 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 abstract 1
- 230000004151 fermentation Effects 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 239000002609 medium Substances 0.000 abstract 1
- 235000019319 peptone Nutrition 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 239000013587 production medium Substances 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 239000011734 sodium Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
- 239000012138 yeast extract Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Materials For Medical Uses (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Przedmiotem zgłoszenia jest sposób wytwarzania modyfikowanej bionanocelulozy (BNC), według wynalazku, polegający na przygotowaniu inokulum poprzez zaszczepienie na podłożu płynnym Hestrin-Schramm zawierającym glukozę, ekstrakt drożdżowy, pepton bakteryjny, kwas cytrynowy, Na<sub>2</sub>HPO<sub>4</sub>, MgSO<sub>4</sub> • H<sub>2</sub>O, w płynnej wysterylizowanej pożywce hodowlanej bakterii fermentacji octowej z rodzaju Komagataeibacter, korzystnie szczepem bakterii Komagataeibacter xylinus. Następnie wymieszaniu przez 15 minut i inkubowaniu przez 7 dni w temperaturze 25 - 30°C, ponownym wymieszaniu przez 5 minut, i, przeniesieniu tak otrzymanego inokulum w ilości 5 – 20% objętościowych do podłoża produkcyjnego i prowadzeniu hodowli stacjonarnej przez 3 - 20 dni w temperaturze 25 - 30°C. Następnym oczyszczaniu za pomocą 0,1 M roztworu NaOH w 80°C przez 30 min i przepłukiwaniu wodą destylowaną do momentu ustabilizowania pH na poziomie 6,5 - 7,5 i wysuszeniu. Istota wynalazku polega na tym, że materiał BNC poddaje się modyfikacji za pomocą plazmy niskociśnieniowej, czyli zjonizowanych cząsteczek gazu przez czas 1 - 30 minut. Proces prowadzi się w generatorze plazmy przy przepływie gazu w zakresie 5 - 50 sccm.The subject of the application is a method of producing modified bionanocellulose (BNC), according to the invention, consisting in the preparation of an inoculum by inoculation on a Hestrin-Schramm liquid medium containing glucose, yeast extract, bacterial peptone, citric acid, Na<sub>2</sub>HPO<sub >4</sub>, MgSO<sub>4</sub> • H<sub>2</sub>O, in a liquid sterilized culture medium of acetic fermentation bacteria of the Komagataeibacter genus, preferably a Komagataeibacter xylinus bacterial strain. Then, mixing for 15 minutes and incubation for 7 days at 25 - 30°C, mixing again for 5 minutes, and transferring the inoculum thus obtained in the amount of 5 - 20% by volume to the production medium and carrying out stationary culture for 3 - 20 days in at 25 - 30°C. Subsequent purification with 0.1 M NaOH solution at 80°C for 30 min and rinsing with distilled water until the pH stabilized at 6.5 - 7.5 and drying. The essence of the invention is that the BNC material is modified using low-pressure plasma, i.e. ionized gas molecules, for 1 - 30 minutes. The process is carried out in a plasma generator with a gas flow in the range of 5 - 50 sccm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL439149A PL243531B1 (en) | 2021-10-07 | 2021-10-07 | Method for producing modified bacterial cellulose with antimicrobial properties |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PL439149A PL243531B1 (en) | 2021-10-07 | 2021-10-07 | Method for producing modified bacterial cellulose with antimicrobial properties |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL439149A1 true PL439149A1 (en) | 2023-04-11 |
| PL243531B1 PL243531B1 (en) | 2023-09-04 |
Family
ID=85983087
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL439149A PL243531B1 (en) | 2021-10-07 | 2021-10-07 | Method for producing modified bacterial cellulose with antimicrobial properties |
Country Status (1)
| Country | Link |
|---|---|
| PL (1) | PL243531B1 (en) |
-
2021
- 2021-10-07 PL PL439149A patent/PL243531B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL243531B1 (en) | 2023-09-04 |
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