PL449273A1 - Fuzyjne białko rekombinowane, sposób otrzymywania fuzyjnego białka rekombinowanego i jego zastosowanie - Google Patents

Fuzyjne białko rekombinowane, sposób otrzymywania fuzyjnego białka rekombinowanego i jego zastosowanie

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Publication number
PL449273A1
PL449273A1 PL449273A PL44927324A PL449273A1 PL 449273 A1 PL449273 A1 PL 449273A1 PL 449273 A PL449273 A PL 449273A PL 44927324 A PL44927324 A PL 44927324A PL 449273 A1 PL449273 A1 PL 449273A1
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PL
Poland
Prior art keywords
application
fusion protein
recombinant fusion
cellulose
derived
Prior art date
Application number
PL449273A
Other languages
English (en)
Other versions
PL249602B1 (pl
Inventor
Joanna Żebrowska
Małgorzata Ponikowska
Piotr Skowron
Piotr Mucha
Original Assignee
Uniwersytet Gdański
Gdański Uniwersytet Medyczny
Politechnika Gdańska
Innovabion Spółka Z Ograniczoną Odpowiedzialnością
Nex.D Spółka Z Ograniczoną Odpowiedzialnością
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Uniwersytet Gdański, Gdański Uniwersytet Medyczny, Politechnika Gdańska, Innovabion Spółka Z Ograniczoną Odpowiedzialnością, Nex.D Spółka Z Ograniczoną Odpowiedzialnością filed Critical Uniwersytet Gdański
Priority to PL449273A priority Critical patent/PL249602B1/pl
Publication of PL449273A1 publication Critical patent/PL449273A1/pl
Publication of PL249602B1 publication Critical patent/PL249602B1/pl

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01092—Peptidoglycan beta-N-acetylmuramidase (3.2.1.92)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Environmental Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Przedmiotem zgłoszenia jest fuzyjne białko rekombinowane oznaczone jako CBD_TP84_28_His o sekwencji obejmującej: a) domenę katalityczną w postaci endolizyny TP84_28_His pochodzącą z bakteriofaga TP-84, b) domenę wiążącą celulozę pochodzącą z celulazy Clostridium cellulovorans o właściwościach bakteriobójczych i zdolności wiązania z celulozą. Zgłoszenie obejmuje także sposób otrzymywania fuzyjnego białka rekombinowanego oznaczonego jako CBD_TP84_28_His na drodze, charakteryzujący się tym, że łączy się endolizynę TP84_28 pochodzącą z bakteriofaga TP-84 z domeną wiążącą celulozę pochodzącą z celulazy Clostridium cellulovorans poprzez fuzję genów kodujących endolizynę TP84_28 i domenę wiążącą celulozę enzymu celulazy z Clostridium cellulovorans. Przedmiotem zgłoszenia jest także zastosowanie fuzyjnego białka rekombinowanego, otrzymanego powyższym sposobem.
PL449273A 2024-07-19 2024-07-19 Fuzyjne białko rekombinowane, sposób otrzymywania fuzyjnego białka rekombinowanego i jego zastosowanie PL249602B1 (pl)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL449273A PL249602B1 (pl) 2024-07-19 2024-07-19 Fuzyjne białko rekombinowane, sposób otrzymywania fuzyjnego białka rekombinowanego i jego zastosowanie

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL449273A PL249602B1 (pl) 2024-07-19 2024-07-19 Fuzyjne białko rekombinowane, sposób otrzymywania fuzyjnego białka rekombinowanego i jego zastosowanie

Publications (2)

Publication Number Publication Date
PL449273A1 true PL449273A1 (pl) 2026-01-26
PL249602B1 PL249602B1 (pl) 2026-05-18

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Family Applications (1)

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PL449273A PL249602B1 (pl) 2024-07-19 2024-07-19 Fuzyjne białko rekombinowane, sposób otrzymywania fuzyjnego białka rekombinowanego i jego zastosowanie

Country Status (1)

Country Link
PL (1) PL249602B1 (pl)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996013524A1 (en) * 1994-10-27 1996-05-09 Yeda Research And Development Co. Ltd. Modified cellulose-binding domain (cbd) proteins and use thereof
PL418712A1 (pl) * 2016-09-15 2018-03-26 Bioventures Institute Spółka Z Ograniczoną Odpowiedzialnością Polinukleotyd kodujący element funkcjonalny pochodzący z genomu bakteriofaga TP-84, wektor ekspresyjny, komórka gospodarza bakteryjnego oraz białko bakteriofaga TP-84 lub jego pochodna

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996013524A1 (en) * 1994-10-27 1996-05-09 Yeda Research And Development Co. Ltd. Modified cellulose-binding domain (cbd) proteins and use thereof
PL418712A1 (pl) * 2016-09-15 2018-03-26 Bioventures Institute Spółka Z Ograniczoną Odpowiedzialnością Polinukleotyd kodujący element funkcjonalny pochodzący z genomu bakteriofaga TP-84, wektor ekspresyjny, komórka gospodarza bakteryjnego oraz białko bakteriofaga TP-84 lub jego pochodna

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
SHOSEYOV, O. ET AL.: "Proc. Natl. Acad. Sci. USA 1992, 89, 3483-3487", PRIMARY SEQUENCE ANALYSIS OF CLOSTRIDIUM CELLULOVORANS CELLULOSE BINDING PROTEIN A. *
ŻEBROWSKA, J. ET AL.: "Int. J. Mol. Sci. 2022, 23, 7612", CLONING AND CHARACTERIZATION OF A THERMOSTABLE ENDOLYSIN OF BACTERIOPHAGE TP-84 AS A POTENTIAL DISINFECTANT AND BIOFILM-REMOVING BIOLOGICAL AGENT. *

Also Published As

Publication number Publication date
PL249602B1 (pl) 2026-05-18

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