PT101590B - PROCESS OF EXTRACTION OF AROMAS OF WINE MUSTS USING SUPERCRITICAL FLUIDS - Google Patents

PROCESS OF EXTRACTION OF AROMAS OF WINE MUSTS USING SUPERCRITICAL FLUIDS Download PDF

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PT101590B
PT101590B PT10159094A PT10159094A PT101590B PT 101590 B PT101590 B PT 101590B PT 10159094 A PT10159094 A PT 10159094A PT 10159094 A PT10159094 A PT 10159094A PT 101590 B PT101590 B PT 101590B
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extraction
musts
aromas
supercritical
solvent
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PT10159094A
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Portuguese (pt)
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PT101590A (en
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Manuel Luis De Magalhaes Ponte
Jose Carlos Cavaco Reves
Edmundo Jose Simoes Go Azevedo
Ana Staack Reis Machado
Susana Maria Melo Fernandes
Jose Antonio Da Silva Lopes
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Jose Maria Da Fonseca Sucessor
Inst De Biolog Ex E Tecnologic
Sociedade Portuguesa De Ar Liq
Tecnologia Quimica E Biollogic
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Priority to PT10159094A priority Critical patent/PT101590B/en
Publication of PT101590A publication Critical patent/PT101590A/en
Publication of PT101590B publication Critical patent/PT101590B/en

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Abstract

The invention relates to a process for the extraction of wine must flavours using supercritical fluids, in particular carbon dioxide. In this process a pressurized gas slightly above its critical temperature and pressure is used as the extraction solvent. Carbon dioxide is specially suitable for this application given that it is harmless and is a good solvent of the aromatic substances which have to be separated from the musts. The extraction can be followed by fractionation of the mixture of flavours, obtained by a method of precipitation by successive decompression of the solvent. This process makes it possible to obtain extracts which are highly enriched in aromatic substances present in small quantities in the musts, extracts which are suitable for flavouring wines.

Description

Processo de extracçao de aromas de mostos de vinho utilizando fluidos super-críticos.Extraction process of wine musts using super-critical fluids.

Figura (para interpretação do resumo)Figure (for summary interpretation)

Resumo: (máx. 150 palavras) A presente invenção de mostos de vinhos dioxido de carbono, acima das suas de extracçao.Abstract: (max. 150 words) The present invention of carbon dioxide wine musts, above their extraction.

refere-se a um processo de extracçao de aromas utilizando fluidos super-críticos, em particular Neste processo um gás sob pressão, ligeiramente temperaturas e pressão criticas, e usado como solvente 0 dioxido de carbono e especialmente adequado para a presente aplicaçao, dado ser inócuo e um bom solvente das substâncias aromáticas a separar dos mostos. A extracçao pode ser seguida por um fraccionamento da mistura de aromas, obtido por um método de precipitação por descompressões sucessivas do solvente. Este processo permite a obtenção de extractos fortemente enriquecidos em substâncias aromaticas presentes em pequenas quantidades nos mostos, extractos estes adequados para a aromatizaçao de vinhos.refers to a process of extracting aromas using super-critical fluids, in particular In this process a gas under pressure, slightly critical temperatures and pressure, and used as a solvent 0 carbon dioxide is especially suitable for the present application, since it is harmless and a good solvent for the aromatic substances to be separated from the musts. The extraction can be followed by a fractionation of the flavor mixture, obtained by a precipitation method by successive decompression of the solvent. This process makes it possible to obtain extracts strongly enriched with aromatic substances present in small quantities in the musts, which are suitable for flavoring wines.

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Memória descritiva deDescriptive memory of

Processo de extracção de aromas de mostos de vinhos por extracção com fluidos supercríticosExtraction process of wine mus aromas by extraction with supercritical fluids

A presente invenção refere-se a umprocesso de extracção d£ aromas; a partir de mostos de vinhos, que utiliza como solventes de extracção, gases sob pressão ligeiramente acima das suas temperatura e pressão críticas, designados por fluidos supercríticos.The present invention relates to a flavor extraction process; from wine musts, which use as extraction solvents, gases under pressure slightly above their critical temperature and pressure, called supercritical fluids.

A Extracção com Fluidos Supercríticos (ESC) é uma técnica de separação em que são usados gases perto do ponto crítico, em condições em que a sua densidade depende fortemente da pressão e temperatura. Estes fluidos supercríticos dispõem assim dum poder solvente facilmente ajustável. Esta propriedade pode ser utilizada para, por exemplo, fraccionar misturas complexas ou separar um produto duma reacção do solvente, por passos sucessivos de descompressão. Combinando algumas das vantagens da destilação e da extracção líquido-líquido com a possibilidade de ser utilizada a temperaturas muito perto da ambiente, a extracção com Fluidos Supercríticos pode substituir processos de separação clássicos, especialmente quando em operações com substâncias que venham a ser incluídas no ciclo biológico humano ou que sejam termicamente instáveis.Extraction with Supercritical Fluids (ESC) is a separation technique in which gases are used near the critical point, under conditions where their density depends heavily on pressure and temperature. These supercritical fluids therefore have an easily adjustable solvent power. This property can be used to, for example, fractionate complex mixtures or separate a product from a solvent reaction by successive decompression steps. Combining some of the advantages of distillation and liquid-liquid extraction with the possibility of being used at temperatures very close to the environment, extraction with Supercritical Fluids can replace classic separation processes, especially when in operations with substances that will be included in the cycle human biological or thermally unstable.

A aplicação destes processos na separação de aromas e sabores com dióxido de carbono supercrítico combina a vantagem do solvente utilizado ser uma substância inócua e largamente utilizada na indústria alimentar com a das baixas temperaturas de trabalho, que asseguram uma degradação química mínima dos produtos.The application of these processes in the separation of aromas and flavors with supercritical carbon dioxide combines the advantage of the solvent used being a harmless substance and widely used in the food industry with that of the low working temperatures, which ensure a minimum chemical degradation of the products.

Por outro lado, o mosto de vinho é um caldo de fermentação cujo conteúdo em aromas e sabores vai variando ao longo do processo fermentativo. Alguns dos componentes aromáticos libertam-se em quantidades apreciáveis e perdem-se na atmosfera, quer devido à sua volatilidade, quer por arrastamento pelo dióxido de carbono produzido durante a fermentação. É possível que a reinserção dalgumas destas substâncias no vinho produzido viesse melhorar as suas qualidades, tomando-o mais aceitável em alguns mercados.On the other hand, the wine must is a fermentation broth whose content in aromas and flavors varies throughout the fermentation process. Some of the aromatic components are released in appreciable quantities and are lost in the atmosphere, either due to their volatility or by entrainment by the carbon dioxide produced during fermentation. It is possible that the reinsertion of some of these substances in the wine produced would improve its qualities, making it more acceptable in some markets.

Foi sugerido por Muller et al.1 que a captura destes componentes fosse realizada por adsorção em carvão activado e posterior desorpção com freon-11.It was suggested by Muller et al. 1 that the capture of these components was carried out by adsorption on activated carbon and subsequent desorption with freon-11.

No entanto, os métodos baseados em adsorção correm sempre o risco do adsorvente libertar componentes próprios ou previamente adsorvidos, sendo os aromas obtidos normalmente pouco fiéis aos originais. A invenção aqui descrita refere-se à extracção destas substâncias aromáticas de mostos de vinhos, utilizando como solventes 'MULLER, C.J., WAHLSTROM, V.L.. FUGELSANG, K.C. Capture and Use of Volatile Flavor Constituents Emitted During Wine Fermentation in Beer and Winc Production, B.H. Gump and D.J. Pruett eds., ACS Symposium Series 536, American Chemical Society, Washington DC (1993) fluidos supercríticos e, em particular, dióxido de carbono supercrítico. Este método evita os riscos inerentes à utilização de adsorventes.However, the adsorption-based methods always run the risk of the adsorbent releasing its own or previously adsorbed components, and the obtained aromas are usually not very faithful to the originals. The invention described here relates to the extraction of these aromatic substances from wine musts, using as solvents' MULLER, C.J., WAHLSTROM, V.L .. FUGELSANG, K.C. Capture and Use of Volatile Flavor Constituents Emitted During Wine Fermentation in Beer and Winc Production, BH Gump and DJ Pruett eds., ACS Symposium Series 536, American Chemical Society, Washington DC (1993) supercritical fluids and, in particular, supercritical carbon dioxide . This method avoids the risks inherent in the use of adsorbents.

Até agora só uma referência pôde ser encontrada na literatura técnica sobre extracção de aromas de vinhos com dióxido de carbono. Trata-se do trabalho de Jolly2, que extraiu aromas de vinhos australianos com dióxido de carbono líquido.So far only one reference has been found in the technical literature on extracting wine aromas with carbon dioxide. This is the work of Jolly 2 , who extracted aromas from Australian wines with liquid carbon dioxide.

O processo aqui apresentado diferencia-se deste porque utiliza dióxido de carbono supercrítico e porque os aromas são retirados do mosto, e portanto ainda no decorrer do processo de fermentação. Trabalhos recentes3, em que se comparam osextractos de aromas de plantas com dióxido de carbono líquido e supercrítico, comprovam a existência de diferenças significativas na capacidade de dissolução dessa substância nos dois estados, sendo o extracto obtido por dióxido de carbono supercrítico muito mais fiel ao aroma original da planta. Os extractos obtidos podem ser posteriormente injectados em vinhos para melhorar as suas propriedades aromáticas.The process presented here differs from this because it uses supercritical carbon dioxide and because the aromas are removed from the wort, and therefore still during the fermentation process. Recent studies 3 , in which plant aroma extracts are compared with liquid and supercritical carbon dioxide, prove the existence of significant differences in the dissolution capacity of this substance in the two states, with the extract obtained by supercritical carbon dioxide being much more faithful to the original aroma of the plant. The extracts obtained can then be injected into wines to improve their aromatic properties.

Os mostos desaromatizados podem continuar o processo de fermentação, dando origem a vinhos menos aromáticos. O processo normalmente utilizado para obter a; J χ/χ?/ desaromatização é a adsorção em carvão activado, que provoca a inutilização dos k, L aromas extraídos, dada a dificuldade de desadsorção acima apontada. Os resíduos de í cuuxL’ '.nL j carvão representam por outro lado um problema ambiental significativo. O processo 1 aqui descrito de extracção supercrítica tem assim a vantagem de desaromatizar os mostos com aproveitamento das substâncias aromáticas e sem problemas de resíduos.Disarmatized musts can continue the fermentation process, giving rise to less aromatic wines. The process normally used to obtain the; J χ / χ? / Desaromatização is the adsorption on activated carbon, which causes the k, L aromas extracted to become unusable, given the difficulty of desorption mentioned above. Waste from coal, on the other hand, represents a significant environmental problem. The process 1 described here of supercritical extraction thus has the advantage of disarming the musts with the use of aromatic substances and without waste problems.

O exemplo seguintes ilustra o processo de extracção inventado:The following example illustrates the extraction process invented:

ExemploExample

Utilizou-se um aparelho constituído essencialmente por um extractor de alta pressão de capacidade 100 cm3, um compressor manual de líquidos e um dispositivo de precipitação do extracto. A tampa do extractor é perfurada por dois tubos, soldados a essa mesma tampa. Um destes é destinado à alimentação de fluido supercrítico, prolongando-se até ao fundo do recipiente, enquanto o outro se destina à saída de solvente e extracto.An apparatus consisting essentially of a 100 cm 3 high-pressure extractor, a manual liquid compressor and an extract precipitation device was used. The extractor cover is pierced by two tubes, welded to the same cover. One of these is intended for the supply of supercritical fluid, extending to the bottom of the container, while the other is intended for the outlet of solvent and extract.

No extractor de alta pressão colocaram-se 40 cm3 de mosto. A tampa do reactor foi ajustada e o conjunto colocado num banho termostático, à temperatura de compressor foi cheio com dióxido de carbono líquido, que foi comprimido feito passar pelo mosto no extractor, a um caudal de cerca de 1 g min*1,40 cm 3 of wort were placed in the high-pressure extractor. The reactor lid was adjusted and the set placed in a thermostatic bath, at the compressor temperature, was filled with liquid carbon dioxide, which was compressed through the wort in the extractor, at a flow rate of about 1 g min * 1 ,

-/ 2JOLLY, D.R. P. Process Biochemistry 1981, 16, 36 3A.S. REIS MACHADO, E. GOMES DE AZEVEDO, R.M. SARDINHA, M. NUNES DA PONTE High Pressure Carbon Dioxide Extraction from Coriander Plants J. Essent. Oil Res., 1993, 5, 645- / 2 JOLLY, DRP Process Biochemistry 1981, 16, 36 3 THE KINGS MACHADO, E. GOMES DE AZEVEDO, RM SARDINHA, M. NUNES DA PONTE High Pressure Carbon Dioxide Extraction from Coriander Plants J. Essent. Oil Res., 1993, 5, 645

A.S. REIS MACHADO, E. GOMES DE AZEVEDO, R.M. SARDINHA, M.NUNES DA PONTE High Pressure Carbon Dioxide Extraction from Geranium Plants J. Essent. Oil Res. 1993, 5, 185.A.S. REIS MACHADO, E. GOMES DE AZEVEDO, R.M.SARDINHA, M.NUNES DA PONTE High Pressure Carbon Dioxide Extraction from Geranium Plants J. Essent. Oil Res. 1993, 5, 185.

previamente aquecido a 40 °C (nestas condições, o dioxidc de carbono é um fluido supercrítico).previously heated to 40 ° C (under these conditions, carbon dioxidc is a supercritical fluid).

Obtiveram-se cromatogramas por técnica dc head-spuce do exíracto e do mosto inicial. Estes cromatogramas evidenciam um grande enriquecimento do extracto em relação ao mosto numa série de substâncias aromáticas, tendo sido identificadas, entre outras: acetato de etilo, isopropamol.,-isobutahQJL.3-metil-2-butaRQL álcool amflico,Chromatograms were obtained by the head-spuce technique of the exirate and the initial must. These chromatograms show a great enrichment of the extract in relation to the wort in a series of aromatic substances, having been identified, among others: ethyl acetate, isopropamol., - isobutahQJL.3-methyl-2-butaRQL alcohol,

1-hexanol, 'Ί-decanol, feniletánol, ' Iinalol, geraniol,' heptanal, octanal, acetato feniletílico, succinato dietílico.1-hexanol, 'Ί-decanol, phenylethanol,' Iinalol, geraniol, 'heptanal, octanal, phenylethyl acetate, diethyl succinate.

Claims (2)

ReivindicaçõesClaims -1Processo novo de extracção de aromas caracterizado por utilizar fluidos supercriticos, em particular dióxido de carbono supercritico, a pressões entre 14 e 20 MPa e temperaturas entre 35 °C e 50 °C, para a extracção de substâncias aromáticas a partir de mostos de vinho entre o segundo e o oitavo dias de fermentação e posterior ffaccionamento-das misturas de aromas obtidas por este processo de extracção supercrítica, utilizando um método de precipitação por descompressões sucessivas do solvente. Deste processo resulta uma mistura rica em ésteres e álcoois superiores, como o feniletanol, de elevado poder aromatizante.-1New flavor extraction process characterized by using supercritical fluids, in particular supercritical carbon dioxide, at pressures between 14 and 20 MPa and temperatures between 35 ° C and 50 ° C, for the extraction of aromatic substances from wine musts between the second and eighth days of fermentation and subsequent fractionation of the flavor mixtures obtained by this supercritical extraction process, using a precipitation method by successive decompression of the solvent. This process results in a mixture rich in esters and higher alcohols, such as phenylethanol, with high flavoring power. -2 Processo novo caracterizado pela obtenção de mostos desaromatizados, de acordo com o processo novo referido na reinvidicação 1, cuja posterior fermentação conduza a vinhos com novas qualidades.-2 New process characterized by obtaining de-aromatised musts, according to the new process referred to in claim 1, whose subsequent fermentation leads to wines with new qualities. -3Utilização das misturas aromáticas obtidas pelo processo descrito na reinvidicação 1, caracterizada por se obterem vinhos aromatizados quer através do contacto directo com a mistura solvente supercrítico-í-aromas após descompressão, quer por adição separada dos aromas precipitados.-3Use of the aromatic mixtures obtained by the process described in claim 1, characterized by obtaining aromatized wines either through direct contact with the supercritical solvent-β-flavor mixture after decompression, or by separately adding the precipitated aromas. -4Utilização dos aromas obtidos de acordo com o processo da reinvidicação 1, caracterizada por se obter a aromatização de outros produtos que não os referidos na reinvidicação 3.-4Use of the aromas obtained according to the claim 1 process, characterized by the aromatization of products other than those mentioned in claim 3.
PT10159094A 1994-10-14 1994-10-14 PROCESS OF EXTRACTION OF AROMAS OF WINE MUSTS USING SUPERCRITICAL FLUIDS PT101590B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004043168A1 (en) * 2002-11-09 2004-05-27 Degussa Ag Method for the selective separation of volatile flavorings from monophase, (semi)liquid starting materials having a fat content and/or oil content of = 20 percent by weight

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* Cited by examiner, † Cited by third party
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ES2168955B1 (en) * 2000-04-06 2003-11-01 Univ Madrid Autonoma CONTINUOUS PROCESS FOR OBTAINING AROMATIC EXTRACTS OF ALCOHOLIC BEVERAGES DISTILLED BY SUPERCRITICAL FLUIDS.

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004043168A1 (en) * 2002-11-09 2004-05-27 Degussa Ag Method for the selective separation of volatile flavorings from monophase, (semi)liquid starting materials having a fat content and/or oil content of = 20 percent by weight

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