RU2012122025A - Плюрипотентные стволовые клетки - Google Patents
Плюрипотентные стволовые клетки Download PDFInfo
- Publication number
- RU2012122025A RU2012122025A RU2012122025/10A RU2012122025A RU2012122025A RU 2012122025 A RU2012122025 A RU 2012122025A RU 2012122025/10 A RU2012122025/10 A RU 2012122025/10A RU 2012122025 A RU2012122025 A RU 2012122025A RU 2012122025 A RU2012122025 A RU 2012122025A
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- Russia
- Prior art keywords
- cells
- pluripotent stem
- stem cells
- feeder
- layer
- Prior art date
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- 210000001778 pluripotent stem cell Anatomy 0.000 title claims abstract 5
- 210000004027 cell Anatomy 0.000 claims abstract 6
- 239000000126 substance Substances 0.000 claims abstract 4
- 238000001890 transfection Methods 0.000 claims abstract 4
- 210000004381 amniotic fluid Anatomy 0.000 claims abstract 2
- 230000000798 anti-retroviral effect Effects 0.000 claims abstract 2
- 238000004519 manufacturing process Methods 0.000 claims abstract 2
- 238000000034 method Methods 0.000 claims abstract 2
- 230000001177 retroviral effect Effects 0.000 claims abstract 2
- 210000001082 somatic cell Anatomy 0.000 claims abstract 2
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- C—CHEMISTRY; METALLURGY
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/602—Sox-2
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/603—Oct-3/4
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/605—Nanog
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/608—Lin28
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases [EC 2.]
- C12N2501/727—Kinases (EC 2.7.)
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- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
- C12N2740/15041—Use of virus, viral particle or viral elements as a vector
- C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C12N2799/00—Uses of viruses
- C12N2799/02—Uses of viruses as vector
- C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
- C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Transplantation (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
1. Способ получения плюрипотентных стволовых клеток из соматических клеток без использования слоя фидерных клеток или вещества, повышающего эффективность антиретровирусной трансфекции.2. Популяция плюрипотентных стволовых клеток, полученных из клеток из амниотической жидкости с помощью метода, не требующего применения фидерного слоя клеток или вещества, повышающего эффективность ретровирусной трансфекции.
Claims (2)
1. Способ получения плюрипотентных стволовых клеток из соматических клеток без использования слоя фидерных клеток или вещества, повышающего эффективность антиретровирусной трансфекции.
2. Популяция плюрипотентных стволовых клеток, полученных из клеток из амниотической жидкости с помощью метода, не требующего применения фидерного слоя клеток или вещества, повышающего эффективность ретровирусной трансфекции.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25614909P | 2009-10-29 | 2009-10-29 | |
| US61/256,149 | 2009-10-29 | ||
| PCT/US2010/054408 WO2011059725A2 (en) | 2009-10-29 | 2010-10-28 | Pluripotent stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2012122025A true RU2012122025A (ru) | 2013-12-10 |
Family
ID=43925858
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2012122025/10A RU2012122025A (ru) | 2009-10-29 | 2010-10-28 | Плюрипотентные стволовые клетки |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US20110104805A1 (ru) |
| EP (1) | EP2494035B1 (ru) |
| JP (2) | JP5957382B2 (ru) |
| KR (1) | KR101861584B1 (ru) |
| CN (2) | CN102695790A (ru) |
| AR (1) | AR078805A1 (ru) |
| AU (1) | AU2010319921A1 (ru) |
| BR (1) | BR112012010136A2 (ru) |
| CA (1) | CA2778817A1 (ru) |
| ES (1) | ES2665006T3 (ru) |
| MX (1) | MX350966B (ru) |
| PH (1) | PH12012500818A1 (ru) |
| PL (1) | PL2494035T3 (ru) |
| RU (1) | RU2012122025A (ru) |
| SG (1) | SG10201500674YA (ru) |
| WO (1) | WO2011059725A2 (ru) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US9080145B2 (en) | 2007-07-01 | 2015-07-14 | Lifescan Corporation | Single pluripotent stem cell culture |
| US9096832B2 (en) | 2007-07-31 | 2015-08-04 | Lifescan, Inc. | Differentiation of human embryonic stem cells |
| CA2959401C (en) | 2008-02-21 | 2021-12-07 | Centocor Ortho Biotech Inc. | Methods, surface modified plates and compositions for cell attachment, cultivation and detachment |
| KR101651661B1 (ko) | 2008-06-30 | 2016-08-26 | 얀센 바이오테크 인코포레이티드 | 만능 줄기 세포의 분화 |
| PL2366022T3 (pl) | 2008-11-20 | 2016-11-30 | Sposoby i kompozycje do przyłączania i hodowli komórek na podłożach planarnych | |
| PL2366021T3 (pl) | 2008-11-20 | 2017-12-29 | Janssen Biotech, Inc. | Hodowla pluripotencjalnych komórek macierzystych na mikronośnikach |
| KR20170118969A (ko) | 2009-07-20 | 2017-10-25 | 얀센 바이오테크 인코포레이티드 | 인간 배아 줄기 세포의 분화 |
| AU2010333839C1 (en) | 2009-12-23 | 2016-07-21 | Janssen Biotech Inc. | Differentiation of human embryonic stem cells |
| CN107189979B (zh) | 2010-03-01 | 2021-05-04 | 詹森生物科技公司 | 纯化衍生自多能干细胞的细胞的方法 |
| AU2011296381B2 (en) | 2010-08-31 | 2016-03-31 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells |
| US9388386B2 (en) | 2011-12-22 | 2016-07-12 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells into single hormonal insulin positive cells |
| US20150064141A1 (en) | 2012-04-05 | 2015-03-05 | The Regents Of The University Of California | Regenerative sera cells and mesenchymal stem cells |
| WO2013184888A1 (en) | 2012-06-08 | 2013-12-12 | Janssen Biotech, Inc. | Differentiation of human embryonic stem cells into pancreatic endocrine cells |
| US10370644B2 (en) | 2012-12-31 | 2019-08-06 | Janssen Biotech, Inc. | Method for making human pluripotent suspension cultures and cells derived therefrom |
| RU2687379C2 (ru) | 2012-12-31 | 2019-05-13 | Янссен Байотек, Инк. | Суспендирование и кластеризация плюрипотентных клеток человека с целью их дифференцировки в панкреатические эндокринные клетки |
| RU2684215C2 (ru) | 2012-12-31 | 2019-04-04 | Янссен Байотек, Инк. | Способ получения панкреатических эндокринных клеток (варианты) и способ увеличения выхода бета-клеток |
| WO2014105543A1 (en) | 2012-12-31 | 2014-07-03 | Janssen Biotech, Inc. | Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells |
| KR102580225B1 (ko) | 2013-06-11 | 2023-09-20 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | SC-β 세포 및 조성물 그리고 그 생성 방법 |
| JP6588969B2 (ja) | 2014-05-16 | 2019-10-09 | ヤンセン バイオテツク,インコーポレーテツド | 膵内分泌細胞内のmafa発現を強化するための小分子の使用 |
| ES2985135T3 (es) | 2014-12-18 | 2024-11-04 | Harvard College | Procedimientos para generar células ß derivadas de células madre y usos de las mismas |
| WO2016100930A1 (en) | 2014-12-18 | 2016-06-23 | President And Fellows Of Harvard College | Methods for generating stem cell-derived b cells and methods of use thereof |
| WO2016100898A1 (en) | 2014-12-18 | 2016-06-23 | President And Fellows Of Harvard College | Serum-free in vitro directed differentiation protocol for generating stem cell-derived b cells and uses thereof |
| EP3234109A4 (en) | 2014-12-19 | 2018-06-27 | Janssen Biotech, Inc. | Suspension culturing of pluripotent stem cells |
| WO2016141460A1 (en) | 2015-03-11 | 2016-09-15 | The University Of British Columbia | Pancreatic endocrine progenitor cell therapies for the treatment of obesity and type 2 diabetes (t2d) |
| CN104988115A (zh) * | 2015-07-14 | 2015-10-21 | 中国科学院过程工程研究所 | 喹啉或异喹啉类小分子化合物在人多能干细胞培养及冷冻保存中的应用 |
| JP6705911B2 (ja) | 2016-04-14 | 2020-06-03 | ヤンセン バイオテツク,インコーポレーテツド | 多能性幹細胞の中腸内胚葉細胞への分化 |
| EP4691479A3 (en) | 2016-06-21 | 2026-04-15 | Janssen Biotech, Inc. | Generation of human pluripotent stem cell derived functional beta cells showing a glucose-dependent mitochondrial respiration and two-phase insulin secretion response |
| US10767164B2 (en) | 2017-03-30 | 2020-09-08 | The Research Foundation For The State University Of New York | Microenvironments for self-assembly of islet organoids from stem cells differentiation |
| CN118497104A (zh) | 2017-11-15 | 2024-08-16 | 沃泰克斯药物股份有限公司 | 胰岛细胞制备性组合物和使用方法 |
| EP3833365A4 (en) | 2018-08-10 | 2022-05-11 | Vertex Pharmaceuticals Incorporated | Stem cell derived islet differentiation |
| CN110564674B (zh) * | 2019-09-29 | 2021-05-11 | 广东工业大学 | 一种干细胞铺底工作液及其应用 |
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-
2010
- 2010-10-28 CN CN2010800497211A patent/CN102695790A/zh active Pending
- 2010-10-28 KR KR1020127013705A patent/KR101861584B1/ko not_active Expired - Fee Related
- 2010-10-28 JP JP2012537026A patent/JP5957382B2/ja not_active Expired - Fee Related
- 2010-10-28 RU RU2012122025/10A patent/RU2012122025A/ru not_active Application Discontinuation
- 2010-10-28 AR ARP100103962A patent/AR078805A1/es unknown
- 2010-10-28 SG SG10201500674YA patent/SG10201500674YA/en unknown
- 2010-10-28 CA CA2778817A patent/CA2778817A1/en not_active Abandoned
- 2010-10-28 PH PH1/2012/500818A patent/PH12012500818A1/en unknown
- 2010-10-28 CN CN201611048756.9A patent/CN107058389A/zh active Pending
- 2010-10-28 WO PCT/US2010/054408 patent/WO2011059725A2/en not_active Ceased
- 2010-10-28 US US12/913,834 patent/US20110104805A1/en not_active Abandoned
- 2010-10-28 EP EP10830437.9A patent/EP2494035B1/en not_active Not-in-force
- 2010-10-28 PL PL10830437T patent/PL2494035T3/pl unknown
- 2010-10-28 BR BR112012010136A patent/BR112012010136A2/pt not_active Application Discontinuation
- 2010-10-28 ES ES10830437.9T patent/ES2665006T3/es active Active
- 2010-10-28 MX MX2012004999A patent/MX350966B/es active IP Right Grant
- 2010-10-28 AU AU2010319921A patent/AU2010319921A1/en not_active Abandoned
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Also Published As
| Publication number | Publication date |
|---|---|
| CN102695790A (zh) | 2012-09-26 |
| MX350966B (es) | 2017-09-26 |
| MX2012004999A (es) | 2012-09-28 |
| KR101861584B1 (ko) | 2018-05-28 |
| US20110104805A1 (en) | 2011-05-05 |
| SG10201500674YA (en) | 2015-04-29 |
| EP2494035A4 (en) | 2013-09-25 |
| KR20120099241A (ko) | 2012-09-07 |
| ES2665006T3 (es) | 2018-04-24 |
| BR112012010136A2 (pt) | 2015-09-15 |
| EP2494035A2 (en) | 2012-09-05 |
| JP5957382B2 (ja) | 2016-07-27 |
| WO2011059725A2 (en) | 2011-05-19 |
| AR078805A1 (es) | 2011-12-07 |
| PH12012500818A1 (en) | 2019-11-29 |
| JP2016127828A (ja) | 2016-07-14 |
| PL2494035T3 (pl) | 2018-07-31 |
| JP2013509184A (ja) | 2013-03-14 |
| CA2778817A1 (en) | 2011-05-19 |
| JP6227622B2 (ja) | 2017-11-08 |
| EP2494035B1 (en) | 2018-02-28 |
| AU2010319921A1 (en) | 2012-05-17 |
| CN107058389A (zh) | 2017-08-18 |
| WO2011059725A3 (en) | 2011-07-21 |
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