RU2112802C1 - Фрагмент днк, кодирующий промотор, обеспечивающий регулируемую кальцием экспрессию полипептидов, рекомбинантный вектор экспрессии pulad 50, рекомбинантный вектор экспрессии pulad 51, рекомбинантный штамм streptomyces lividans - продуцент аминогликозидфосфотрансферазы, рекомбинантный штамм streptomyces lividans - продуцент катехолдезоксигеназы, способ экспрессии чужеродной днк-последовательности - Google Patents

Фрагмент днк, кодирующий промотор, обеспечивающий регулируемую кальцием экспрессию полипептидов, рекомбинантный вектор экспрессии pulad 50, рекомбинантный вектор экспрессии pulad 51, рекомбинантный штамм streptomyces lividans - продуцент аминогликозидфосфотрансферазы, рекомбинантный штамм streptomyces lividans - продуцент катехолдезоксигеназы, способ экспрессии чужеродной днк-последовательности Download PDF

Info

Publication number
RU2112802C1
RU2112802C1 RU92004585A RU92004585A RU2112802C1 RU 2112802 C1 RU2112802 C1 RU 2112802C1 RU 92004585 A RU92004585 A RU 92004585A RU 92004585 A RU92004585 A RU 92004585A RU 2112802 C1 RU2112802 C1 RU 2112802C1
Authority
RU
Russia
Prior art keywords
promoter
pulad
expression
dna
recombinant
Prior art date
Application number
RU92004585A
Other languages
English (en)
Russian (ru)
Other versions
RU92004585A (ru
Inventor
Даса Ортега Хосе
Антонио Гил Хосе
Вигал Гарсиа Томас
Франсиско Мартин Хуан
Original Assignee
Лабораториос Сероно С.А.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Лабораториос Сероно С.А. filed Critical Лабораториос Сероно С.А.
Publication of RU92004585A publication Critical patent/RU92004585A/ru
Application granted granted Critical
Publication of RU2112802C1 publication Critical patent/RU2112802C1/ru

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/001Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y113/00Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/11Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
    • C12Y113/11001Catechol 1,2-dioxygenase (1.13.11.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y113/00Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/11Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
    • C12Y113/11002Catechol 2,3-dioxygenase (1.13.11.2)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Fodder In General (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Hydrogenated Pyridines (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Dental Preparations (AREA)
RU92004585A 1991-12-12 1992-12-11 Фрагмент днк, кодирующий промотор, обеспечивающий регулируемую кальцием экспрессию полипептидов, рекомбинантный вектор экспрессии pulad 50, рекомбинантный вектор экспрессии pulad 51, рекомбинантный штамм streptomyces lividans - продуцент аминогликозидфосфотрансферазы, рекомбинантный штамм streptomyces lividans - продуцент катехолдезоксигеназы, способ экспрессии чужеродной днк-последовательности RU2112802C1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ESP9102765 1991-12-12
ES9102765 1991-12-12

Publications (2)

Publication Number Publication Date
RU92004585A RU92004585A (ru) 1996-09-20
RU2112802C1 true RU2112802C1 (ru) 1998-06-10

Family

ID=8274462

Family Applications (1)

Application Number Title Priority Date Filing Date
RU92004585A RU2112802C1 (ru) 1991-12-12 1992-12-11 Фрагмент днк, кодирующий промотор, обеспечивающий регулируемую кальцием экспрессию полипептидов, рекомбинантный вектор экспрессии pulad 50, рекомбинантный вектор экспрессии pulad 51, рекомбинантный штамм streptomyces lividans - продуцент аминогликозидфосфотрансферазы, рекомбинантный штамм streptomyces lividans - продуцент катехолдезоксигеназы, способ экспрессии чужеродной днк-последовательности

Country Status (15)

Country Link
US (2) US5385841A (fr)
EP (1) EP0546833B1 (fr)
JP (1) JP3340164B2 (fr)
AT (1) ATE154392T1 (fr)
AU (1) AU661073B2 (fr)
CA (1) CA2084351C (fr)
DE (1) DE69220336T2 (fr)
DK (1) DK0546833T3 (fr)
ES (1) ES2103902T3 (fr)
GR (1) GR3024625T3 (fr)
IL (1) IL103975A (fr)
LT (1) LT3133B (fr)
LV (1) LV10728B (fr)
RU (1) RU2112802C1 (fr)
ZA (1) ZA929284B (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA929284B (en) * 1991-12-12 1993-06-02 Serono Lab P0438, an new calcium-regulated promoter.
US6381353B1 (en) 1996-08-30 2002-04-30 Marvin Weiss System for counting colonies of micro-organisms in petri dishes and other culture media

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8315579D0 (en) * 1983-06-07 1983-07-13 Biogen Nv Streptomyces expression systems
US4766066A (en) * 1984-09-27 1988-08-23 Eli Lilly And Company Method of using bacteriophage lambda p1 promoter to produce a functional polypeptide in streptomyces
US4745056A (en) * 1984-10-23 1988-05-17 Biotechnica International, Inc. Streptomyces secretion vector
DE3536182A1 (de) * 1985-10-10 1987-04-16 Hoechst Ag Promotor-anordnung fuer streptomyceten-vektoren
US5200327A (en) * 1985-11-06 1993-04-06 Cangene Corporation Expression system for the secretion of bioactive human granulocyte macrophage colony stimulating factor (GM-CSF) and other heterologous proteins from streptomyces
AU1299988A (en) 1987-03-09 1988-10-10 Cetus Corporation Expression of heterologous genes in streptomyces species
US5118617A (en) * 1989-05-19 1992-06-02 Applied Research Systems Ars Holding N.V. Saf polypeptide, gene coding therefor, saf promoter and expression methods for expressing foreign dna sequences in streptomyces
ZA929284B (en) * 1991-12-12 1993-06-02 Serono Lab P0438, an new calcium-regulated promoter.

Also Published As

Publication number Publication date
IL103975A0 (en) 1993-05-13
CA2084351C (fr) 2007-04-10
DE69220336T2 (de) 1997-09-25
EP0546833A3 (fr) 1994-04-06
JP3340164B2 (ja) 2002-11-05
US5660999A (en) 1997-08-26
ZA929284B (en) 1993-06-02
AU2979192A (en) 1993-06-17
LV10728B (en) 1995-12-20
CA2084351A1 (fr) 1993-06-13
LTIP237A (en) 1994-07-15
AU661073B2 (en) 1995-07-13
EP0546833B1 (fr) 1997-06-11
LV10728A (lv) 1995-06-20
LT3133B (en) 1995-01-31
ES2103902T3 (es) 1997-10-01
US5385841A (en) 1995-01-31
DE69220336D1 (de) 1997-07-17
JPH05336972A (ja) 1993-12-21
DK0546833T3 (da) 1997-09-15
ATE154392T1 (de) 1997-06-15
GR3024625T3 (es) 1997-12-31
IL103975A (en) 1998-08-16
EP0546833A2 (fr) 1993-06-16

Similar Documents

Publication Publication Date Title
Ramón et al. Cloning and characterization of the isopenicillin N synthetase gene mediating the formation of the β-lactam ring in Aspergillus nidulans
Steiert et al. A Gene Coding for a Membrane–Bound Hydrolase is Expressed as a Secreted, Soluble Enzyme in Streptomyces Lividans
Hulett et al. Evidence for two structural genes for alkaline phosphatase in Bacillus subtilis
JPH04507346A (ja) アルカリ性タンパク質分解酵素およびその製造方法
Li et al. Cloning, purification, and properties of a phosphotyrosine protein phosphatase from Streptomyces coelicolor A3 (2)
Fernández-Cañón et al. Overexpression of two penicillin structural genes in Aspergillus nidulans
CN111117942B (zh) 一种产林可霉素的基因工程菌及其构建方法和应用
RU2112802C1 (ru) Фрагмент днк, кодирующий промотор, обеспечивающий регулируемую кальцием экспрессию полипептидов, рекомбинантный вектор экспрессии pulad 50, рекомбинантный вектор экспрессии pulad 51, рекомбинантный штамм streptomyces lividans - продуцент аминогликозидфосфотрансферазы, рекомбинантный штамм streptomyces lividans - продуцент катехолдезоксигеназы, способ экспрессии чужеродной днк-последовательности
Schlüter et al. The Rhizobium leguminosarum FnrN protein is functionally similar to Escherichia coli Fnr and promotes heterologous oxygen‐dependent activation of transcription
EP0429600B1 (fr) Polypeptide saf, gene, promoteur et procedes d'expression dans le streptomyces
EP0498827B1 (fr) Vecteurs de plasmide ameliores pour myxomycetes cellulaires du gene dictyostelium
Hanau et al. Nucleotide sequence of the control regions for the glnA and glnL genes of Salmonella typhimurium
MX2013010872A (es) Sistemas de expresion genica regulada y construcciones de ellos.
KR19990014759A (ko) 플라스미드
US7718396B2 (en) Inducible high expression system
RU2114910C1 (ru) Фрагмент днк, полученный путем молекулярного клонирования из streptomyces griseus атсс 10137, кодирующий saf-полипептид, saf-полипептид, рекомбинантная плазмидная днк, определяющая экспрессию saf-полипептида (варианты), рекомбинантная плазмидная днк pulad3, рекомбинантная плазмидная днк pulad 300, штамм грибов streptomyces lividans, содержащий рекомбинантную плазмидную днк pulad3, и способ экспрессии saf-полипептида
JPH07102140B2 (ja) 組換えdna
Tsao et al. Cloning and characterization of an α-amylase gene from Streptomyces lividans
RU2221868C2 (ru) Ген l-аспарагиназы erwinia carotovora и штамм escherichia coli вкпм № в-8174 - продуцент l-аспарагиназы erwinia carotovora
US4865982A (en) Cloned streptomycete gene
CN101407822A (zh) 一种双精氨酸途径蛋白质分泌载体的构建方法及其应用
AU649976B2 (en) Improved plasmid vectors for cellular slime moulds of the genus dictyostelium
EP3676370B1 (fr) Compositions et procédés utilisant des protéines de couche s méthanotrophe pour l'expression de protéines hétérologues
JP2537764B2 (ja) 高発現ベクタ―、バチルス属細菌、およびペプチド又は蛋白質の製造方法
DeSanti The molecular biology of the Streptomyces snp locus

Legal Events

Date Code Title Description
MM4A The patent is invalid due to non-payment of fees

Effective date: 20081212