SE455704B - Bakteriecell innehallande plasmid med stabiliserad nedervning, plasmid samt forfarande for framstellning av bakteriecellen - Google Patents

Bakteriecell innehallande plasmid med stabiliserad nedervning, plasmid samt forfarande for framstellning av bakteriecellen

Info

Publication number: SE455704B
Authority: SE; Sweden
Prior art keywords: gene; plasmid; production; nucleic acid; bacterial cell
Prior art date: 1983-10-24

Application number

SE8305838A

Other languages

English (en)

Swedish (sv)

Other versions

SE8305838D0 (sv

SE8305838L (sv

Inventor

L A J Nilsson

S G Skogman

Original Assignee

Ac Biotechnics Ab

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-10-24

Filing date

1983-10-24

Publication date

1988-08-01

1983-10-24 Application filed by Ac Biotechnics Ab filed Critical Ac Biotechnics Ab

1983-10-24 Priority to SE8305838A priority Critical patent/SE455704B/sv

1983-10-24 Publication of SE8305838D0 publication Critical patent/SE8305838D0/xx

1984-10-19 Priority to GB08426538A priority patent/GB2148308B/en

1984-10-22 Priority to EP84850313A priority patent/EP0141790A3/fr

1984-10-23 Priority to JP59222855A priority patent/JPS60110293A/ja

1984-10-24 Priority to US06/664,435 priority patent/US4788148A/en

1985-04-25 Publication of SE8305838L publication Critical patent/SE8305838L/

1988-08-01 Publication of SE455704B publication Critical patent/SE455704B/sv

Links

239000013612 plasmid Substances 0.000 title claims description 50
238000000034 method Methods 0.000 title claims description 12
230000006378 damage Effects 0.000 title 1
108090000623 proteins and genes Proteins 0.000 claims description 29
238000004519 manufacturing process Methods 0.000 claims description 22
230000000694 effects Effects 0.000 claims description 17
101000787278 Arabidopsis thaliana Valine-tRNA ligase, chloroplastic/mitochondrial 2 Proteins 0.000 claims description 15
101000787296 Arabidopsis thaliana Valine-tRNA ligase, mitochondrial 1 Proteins 0.000 claims description 15
101000787280 Dictyostelium discoideum Probable valine-tRNA ligase, mitochondrial Proteins 0.000 claims description 15
102000013625 Valine-tRNA Ligase Human genes 0.000 claims description 14
150000007523 nucleic acids Chemical group 0.000 claims description 13
230000001580 bacterial effect Effects 0.000 claims description 8
108091008146 restriction endonucleases Proteins 0.000 claims description 8
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims description 4
QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 4
241000588724 Escherichia coli Species 0.000 claims description 3
102000003960 Ligases Human genes 0.000 claims description 3
108090000364 Ligases Proteins 0.000 claims description 3
230000035772 mutation Effects 0.000 claims description 3
230000003362 replicative effect Effects 0.000 claims description 3
150000001413 amino acids Chemical class 0.000 claims description 2
230000001404 mediated effect Effects 0.000 claims description 2
229930010796 primary metabolite Natural products 0.000 claims description 2
108020004707 nucleic acids Proteins 0.000 claims 2
102000039446 nucleic acids Human genes 0.000 claims 2
201000003373 familial cold autoinflammatory syndrome 3 Diseases 0.000 claims 1
101150112181 valS gene Proteins 0.000 description 8
230000003115 biocidal effect Effects 0.000 description 7
239000002609 medium Substances 0.000 description 7
XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
239000000203 mixture Substances 0.000 description 6
108010037870 Anthranilate Synthase Proteins 0.000 description 5
239000012634 fragment Substances 0.000 description 5
ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 4
RWZYAGGXGHYGMB-UHFFFAOYSA-N anthranilic acid Chemical compound NC1=CC=CC=C1C(O)=O RWZYAGGXGHYGMB-UHFFFAOYSA-N 0.000 description 4
238000011534 incubation Methods 0.000 description 4
ACWBQPMHZXGDFX-QFIPXVFZSA-N valsartan Chemical compound C1=CC(CN(C(=O)CCCC)[C@@H](C(C)C)C(O)=O)=CC=C1C1=CC=CC=C1C1=NN=NN1 ACWBQPMHZXGDFX-QFIPXVFZSA-N 0.000 description 4
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
102000004190 Enzymes Human genes 0.000 description 3
108090000790 Enzymes Proteins 0.000 description 3
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
239000003242 anti bacterial agent Substances 0.000 description 3
230000029087 digestion Effects 0.000 description 3
229940088598 enzyme Drugs 0.000 description 3
239000008103 glucose Substances 0.000 description 3
238000002955 isolation Methods 0.000 description 3
239000006166 lysate Substances 0.000 description 3
239000011541 reaction mixture Substances 0.000 description 3
238000012546 transfer Methods 0.000 description 3
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
238000000246 agarose gel electrophoresis Methods 0.000 description 2
229960000723 ampicillin Drugs 0.000 description 2
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
238000004458 analytical method Methods 0.000 description 2
229940088710 antibiotic agent Drugs 0.000 description 2
238000003556 assay Methods 0.000 description 2
AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 2
238000005119 centrifugation Methods 0.000 description 2
239000000499 gel Substances 0.000 description 2
230000009466 transformation Effects 0.000 description 2
229940035893 uracil Drugs 0.000 description 2
WTFXTQVDAKGDEY-UHFFFAOYSA-N (-)-chorismic acid Natural products OC1C=CC(C(O)=O)=CC1OC(=C)C(O)=O WTFXTQVDAKGDEY-UHFFFAOYSA-N 0.000 description 1
QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
229920001817 Agar Polymers 0.000 description 1
229920000936 Agarose Polymers 0.000 description 1
235000010585 Ammi visnaga Nutrition 0.000 description 1
244000153158 Ammi visnaga Species 0.000 description 1
USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 1
239000005695 Ammonium acetate Substances 0.000 description 1
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
102000012410 DNA Ligases Human genes 0.000 description 1
108010061982 DNA Ligases Proteins 0.000 description 1
ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
229930182816 L-glutamine Natural products 0.000 description 1
102000016943 Muramidase Human genes 0.000 description 1
108010014251 Muramidase Proteins 0.000 description 1
108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
101100354953 Treponema denticola (strain ATCC 35405 / DSM 14222 / CIP 103919 / JCM 8153 / KCTC 15104) pyrBI gene Proteins 0.000 description 1
239000007983 Tris buffer Substances 0.000 description 1
229920004890 Triton X-100 Polymers 0.000 description 1
239000013504 Triton X-100 Substances 0.000 description 1
239000008272 agar Substances 0.000 description 1
235000019257 ammonium acetate Nutrition 0.000 description 1
229940043376 ammonium acetate Drugs 0.000 description 1
229910052788 barium Inorganic materials 0.000 description 1
DSAJWYNOEDNPEQ-UHFFFAOYSA-N barium atom Chemical compound [Ba] DSAJWYNOEDNPEQ-UHFFFAOYSA-N 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
239000000872 buffer Substances 0.000 description 1
229910052799 carbon Inorganic materials 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
WTFXTQVDAKGDEY-HTQZYQBOSA-N chorismic acid Chemical compound O[C@@H]1C=CC(C(O)=O)=C[C@H]1OC(=C)C(O)=O WTFXTQVDAKGDEY-HTQZYQBOSA-N 0.000 description 1
230000002759 chromosomal effect Effects 0.000 description 1
238000010276 construction Methods 0.000 description 1
238000011109 contamination Methods 0.000 description 1
238000001816 cooling Methods 0.000 description 1
230000007423 decrease Effects 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
238000007599 discharging Methods 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
230000005284 excitation Effects 0.000 description 1
230000001747 exhibiting effect Effects 0.000 description 1
238000000855 fermentation Methods 0.000 description 1
230000004151 fermentation Effects 0.000 description 1
239000012467 final product Substances 0.000 description 1
238000012252 genetic analysis Methods 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
230000001320 lysogenic effect Effects 0.000 description 1
229960000274 lysozyme Drugs 0.000 description 1
239000004325 lysozyme Substances 0.000 description 1
235000010335 lysozyme Nutrition 0.000 description 1
UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 1
239000000463 material Substances 0.000 description 1
230000013011 mating Effects 0.000 description 1
238000002156 mixing Methods 0.000 description 1
239000008188 pellet Substances 0.000 description 1
239000000047 product Substances 0.000 description 1
238000000746 purification Methods 0.000 description 1
101150098691 pyrB gene Proteins 0.000 description 1
239000006152 selective media Substances 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
239000000243 solution Substances 0.000 description 1
238000013112 stability test Methods 0.000 description 1
230000000087 stabilizing effect Effects 0.000 description 1
239000000126 substance Substances 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
238000003786 synthesis reaction Methods 0.000 description 1
238000010361 transduction Methods 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
239000002351 wastewater Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression

Landscapes

Genetics & Genomics (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Wood Science & Technology (AREA)
Organic Chemistry (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Saccharide Compounds (AREA)
Enzymes And Modification Thereof (AREA)

SE8305838A 1983-10-24 1983-10-24 Bakteriecell innehallande plasmid med stabiliserad nedervning, plasmid samt forfarande for framstellning av bakteriecellen SE455704B (sv)

Priority Applications (5)

Application Number	Priority Date	Filing Date	Title
SE8305838A SE455704B (sv)	1983-10-24	1983-10-24	Bakteriecell innehallande plasmid med stabiliserad nedervning, plasmid samt forfarande for framstellning av bakteriecellen
GB08426538A GB2148308B (en)	1983-10-24	1984-10-19	A plasmid with stabilized inheritance
EP84850313A EP0141790A3 (fr)	1983-10-24	1984-10-22	Plasmide à hérédité stabilisée
JP59222855A JPS60110293A (ja)	1983-10-24	1984-10-23	安定化された遺伝質を有するプラスミド
US06/664,435 US4788148A (en)	1983-10-24	1984-10-24	Plasmid with stabilized inheritance

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
SE8305838A SE455704B (sv)	1983-10-24	1983-10-24	Bakteriecell innehallande plasmid med stabiliserad nedervning, plasmid samt forfarande for framstellning av bakteriecellen

Publications (3)

Publication Number	Publication Date
SE8305838D0 SE8305838D0 (sv)	1983-10-24
SE8305838L SE8305838L (sv)	1985-04-25
SE455704B true SE455704B (sv)	1988-08-01

Family

ID=20353025

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
SE8305838A SE455704B (sv)	1983-10-24	1983-10-24	Bakteriecell innehallande plasmid med stabiliserad nedervning, plasmid samt forfarande for framstellning av bakteriecellen

Country Status (5)

Country	Link
US (1)	US4788148A (fr)
EP (1)	EP0141790A3 (fr)
JP (1)	JPS60110293A (fr)
GB (1)	GB2148308B (fr)
SE (1)	SE455704B (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU618640B2 (en)	1988-11-11	1992-01-02	Boehringer Mannheim Gmbh	Process for the expression of a recombinant gene
US5798240A (en) *	1994-09-13	1998-08-25	Cubist Pharmaceuticals, Inc.	Recombinant mycobacterial methionyl-tRNA synthetase genes and methods of use therefore
US5912140A (en) *	1995-04-03	1999-06-15	Cubist Pharmaceuticals, Inc.	Recombinant pneumocystis carinii aminoacyl tRNA synthetase genes, tester strains and assays
US5801013A (en) *	1995-05-26	1998-09-01	Cubist Pharmaceuticals, Inc.	Helicobacter aminoacyl-tRNA synthetase proteins, nucleic acids and strains comprising same
US5885815A (en) *	1996-11-01	1999-03-23	Cubist Pharmaceuticals, Inc.	Candida isoleucyl-tRNA synthetase proteins, nucleic acids and strains comprising same
US5871987A (en) *	1996-11-01	1999-02-16	Cubist Pharmaceuticals, Inc.	Candida tyrosyl-tRNA synthetase proteins, nucleic acids and strains comprising same
US6221640B1 (en)	1997-05-14	2001-04-24	Cubist Pharmaceuticals, Inc.	Enterococcal aminoacyl-trna synthetase proteins, nucleic acids and strains comprising same
US6174713B1 (en)	1997-06-16	2001-01-16	Cubist Pharmaceuticals, Inc.	Candida cytoplasmic tryptophanyl-tRNA synthetase proteins, nucleic acids and strains comprising same

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4237224A (en) *	1974-11-04	1980-12-02	Board Of Trustees Of The Leland Stanford Jr. University	Process for producing biologically functional molecular chimeras
JPS55156591A (en) *	1979-05-23	1980-12-05	Ajinomoto Co Inc	Method for stabilizing property of bacteria containing plasmid
US4371614A (en) *	1980-08-22	1983-02-01	Ajinomoto Co., Inc.	E.Coli bacteria carrying recombinant plasmids and their use in the fermentative production of L-tryptophan
US4436815A (en) *	1981-11-27	1984-03-13	Eli Lilly And Company	Method for stabilizing and selecting recombinant DNA containing host cells
US4654307A (en) *	1983-02-17	1987-03-31	The Research Foundation Of State University Of New York	Novel bacteria containing a plasmid having a tRNA code

1983
- 1983-10-24 SE SE8305838A patent/SE455704B/sv not_active Application Discontinuation
1984
- 1984-10-19 GB GB08426538A patent/GB2148308B/en not_active Expired
- 1984-10-22 EP EP84850313A patent/EP0141790A3/fr not_active Withdrawn
- 1984-10-23 JP JP59222855A patent/JPS60110293A/ja active Pending
- 1984-10-24 US US06/664,435 patent/US4788148A/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
US4788148A (en)	1988-11-29
GB8426538D0 (en)	1984-11-28
EP0141790A2 (fr)	1985-05-15
SE8305838D0 (sv)	1983-10-24
GB2148308A (en)	1985-05-30
JPS60110293A (ja)	1985-06-15
GB2148308B (en)	1987-02-04
EP0141790A3 (fr)	1987-05-13
SE8305838L (sv)	1985-04-25

Legal Events

Date

Code

Title

Description

1995-02-28

NAV

Patent application has lapsed

Ref document number: 8305838-8

Effective date: 19900719

Publication	Publication Date	Title
Molin et al.	1980	Control of plasmid R1 replication: functions involved in replication, copy number control, incompatibility, and switch-off of replication
DiNardo et al.	1982	Escherichia coli DNA topoisomerase I mutants have compensatory mutations in DNA gyrase genes
US5981281A (en)	1999-11-09	Method for knockout mutagenesis in Streptococcus pneumoniae
AU624353B2 (en)	1992-06-11	Process for integration of a chosen gene on the chromosome of a bacterium and bacterium obtained by the said process
US20180127759A1 (en)	2018-05-10	Dynamic genome engineering
US4626504A (en)	1986-12-02	DNA transfer vector for gram-negative bacteria
Boman et al.	1968	Resistance of Escherichia coli to penicillins: IV. Genetic study of mutants resistant to D, L-ampicillin concentrations of 100 μg/ml.
US4806471A (en)	1989-02-21	Plasmids with conditional uncontrolled replication behavior
EP0057976A2 (fr)	1982-08-18	Procédé pour cloner le gène codant pour une alpha-amylase thermostable dans escherichia coli et bacillus subtilis
SE455704B (sv)	1988-08-01	Bakteriecell innehallande plasmid med stabiliserad nedervning, plasmid samt forfarande for framstellning av bakteriecellen
Skogman et al.	1984	Temperature-dependent retention of a tryptophan-operon-bearing plasmid in Escherichia coli
Sako et al.	1980	Coordinate expression of Escherichia coli dnaA and dnaN genes
Davey et al.	1984	The use of mini-Gal plasmids for rapid incompatibility grouping of conjugative R plasmids
CN111548980B (zh)	2022-09-20	一种重组红霉素工程菌及其构建方法和筛选方法和应用
JPS62155081A (ja)	1987-07-10	新規微生物およびそれを用いる醗酵法によるビオチンの製造法
US20120015426A1 (en)	2012-01-19	Bacteria for high efficiency cloning
Hilliker et al.	1978	The activity of Salmonella phage P22 gene 24 product in Escherichia coli
Inoue et al.	1983	pMS76, a plasmid capable of amplification by treatment with chloramphenicol
CN120683099A (zh)	2025-09-23	一种用于链霉菌的高效基因编辑系统及其构建方法和应用
Beher et al.	1981	Coliphage which requires either the LamB protein or the OmpC protein for adsorption to Escherichia coli K-12
Young et al.	1980	Transposition of the kanamycin-resistance transposon Tn903
Mukai et al.	1976	Cloning bacterial genes with plasmid λdv
CN116004689A (zh)	2023-04-25	一种清除双歧杆菌抗性质粒的基因编辑工具及其应用方法
US4845031A (en)	1989-07-04	Recombinant DNA process for producing useful polypeptides
CN1057567C (zh)	2000-10-18	克隆和/或表达的新载体其制备方法及应用