SE500453C2 - Ett in vitro förfarande för utvärdering av en substans effekter - Google Patents
Ett in vitro förfarande för utvärdering av en substans effekterInfo
- Publication number
- SE500453C2 SE500453C2 SE9102901A SE9102901A SE500453C2 SE 500453 C2 SE500453 C2 SE 500453C2 SE 9102901 A SE9102901 A SE 9102901A SE 9102901 A SE9102901 A SE 9102901A SE 500453 C2 SE500453 C2 SE 500453C2
- Authority
- SE
- Sweden
- Prior art keywords
- cells
- solvent
- hormone
- receptors
- containers
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 27
- 239000000126 substance Substances 0.000 title claims abstract description 21
- 238000000338 in vitro Methods 0.000 title claims abstract description 4
- 230000000694 effects Effects 0.000 title description 14
- 238000011156 evaluation Methods 0.000 title description 3
- 229940088597 hormone Drugs 0.000 claims abstract description 36
- 239000005556 hormone Substances 0.000 claims abstract description 36
- 238000012360 testing method Methods 0.000 claims abstract description 22
- 239000000556 agonist Substances 0.000 claims abstract description 16
- 239000005557 antagonist Substances 0.000 claims abstract description 16
- 230000036755 cellular response Effects 0.000 claims abstract description 15
- 102000005962 receptors Human genes 0.000 claims abstract description 15
- 108020003175 receptors Proteins 0.000 claims abstract description 15
- 108091008039 hormone receptors Proteins 0.000 claims abstract description 11
- 239000013558 reference substance Substances 0.000 claims abstract description 11
- 230000001270 agonistic effect Effects 0.000 claims abstract description 10
- 230000003042 antagnostic effect Effects 0.000 claims abstract description 10
- 230000003834 intracellular effect Effects 0.000 claims abstract description 9
- 230000010799 Receptor Interactions Effects 0.000 claims abstract description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 27
- 239000002904 solvent Substances 0.000 claims description 27
- 102000004169 proteins and genes Human genes 0.000 claims description 13
- 230000001105 regulatory effect Effects 0.000 claims description 13
- 230000014509 gene expression Effects 0.000 claims description 11
- 238000004458 analytical method Methods 0.000 claims description 8
- 210000001519 tissue Anatomy 0.000 claims description 8
- 210000000481 breast Anatomy 0.000 claims description 7
- 210000004185 liver Anatomy 0.000 claims description 7
- 210000000988 bone and bone Anatomy 0.000 claims description 6
- 102000015694 estrogen receptors Human genes 0.000 claims description 6
- 108010038795 estrogen receptors Proteins 0.000 claims description 6
- 210000004696 endometrium Anatomy 0.000 claims description 5
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- 108020003113 steroid hormone receptors Proteins 0.000 claims description 5
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- 108090000721 thyroid hormone receptors Proteins 0.000 claims description 4
- 230000001419 dependent effect Effects 0.000 claims description 3
- 239000003862 glucocorticoid Substances 0.000 claims description 3
- 108050001049 Extracellular proteins Proteins 0.000 claims description 2
- 230000010261 cell growth Effects 0.000 claims description 2
- 108050000156 vitamin D receptors Proteins 0.000 claims description 2
- 102000009310 vitamin D receptors Human genes 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 63
- NKANXQFJJICGDU-QPLCGJKRSA-N Tamoxifen Chemical compound C=1C=CC=CC=1C(/CC)=C(C=1C=CC(OCCN(C)C)=CC=1)/C1=CC=CC=C1 NKANXQFJJICGDU-QPLCGJKRSA-N 0.000 description 58
- 229960001603 tamoxifen Drugs 0.000 description 29
- 239000002609 medium Substances 0.000 description 27
- 238000007792 addition Methods 0.000 description 14
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 13
- 102100039175 Trefoil factor 1 Human genes 0.000 description 12
- 229930182833 estradiol Natural products 0.000 description 12
- 229960005309 estradiol Drugs 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 10
- 206010006187 Breast cancer Diseases 0.000 description 9
- 208000026310 Breast neoplasm Diseases 0.000 description 9
- 102000040945 Transcription factor Human genes 0.000 description 7
- 108091023040 Transcription factor Proteins 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 6
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 6
- 229940011871 estrogen Drugs 0.000 description 5
- 239000000262 estrogen Substances 0.000 description 5
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 4
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical class IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 239000012894 fetal calf serum Substances 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 210000004962 mammalian cell Anatomy 0.000 description 3
- 150000003431 steroids Chemical class 0.000 description 3
- 239000005495 thyroid hormone Substances 0.000 description 3
- 229940036555 thyroid hormone Drugs 0.000 description 3
- FCEHBMOGCRZNNI-UHFFFAOYSA-N 1-benzothiophene Chemical compound C1=CC=C2SC=CC2=C1 FCEHBMOGCRZNNI-UHFFFAOYSA-N 0.000 description 2
- 102000003908 Cathepsin D Human genes 0.000 description 2
- 108090000258 Cathepsin D Proteins 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 108090000079 Glucocorticoid Receptors Proteins 0.000 description 2
- 108010088412 Trefoil Factor-1 Proteins 0.000 description 2
- 102000008817 Trefoil Factor-1 Human genes 0.000 description 2
- 229940046836 anti-estrogen Drugs 0.000 description 2
- 230000001833 anti-estrogenic effect Effects 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- DIOQZVSQGTUSAI-UHFFFAOYSA-N decane Chemical compound CCCCCCCCCC DIOQZVSQGTUSAI-UHFFFAOYSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000000328 estrogen antagonist Substances 0.000 description 2
- 230000001076 estrogenic effect Effects 0.000 description 2
- 238000010324 immunological assay Methods 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 230000010534 mechanism of action Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000003270 steroid hormone Substances 0.000 description 2
- 229940037128 systemic glucocorticoids Drugs 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- 102000001301 EGF receptor Human genes 0.000 description 1
- 108060006698 EGF receptor Proteins 0.000 description 1
- 102000003676 Glucocorticoid Receptors Human genes 0.000 description 1
- 102100033417 Glucocorticoid receptor Human genes 0.000 description 1
- 102100020948 Growth hormone receptor Human genes 0.000 description 1
- 102000048143 Insulin-Like Growth Factor II Human genes 0.000 description 1
- 108090001117 Insulin-Like Growth Factor II Proteins 0.000 description 1
- 108010001831 LDL receptors Proteins 0.000 description 1
- 102100024640 Low-density lipoprotein receptor Human genes 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241001036794 Microsorum maximum Species 0.000 description 1
- 102000005604 Myosin Heavy Chains Human genes 0.000 description 1
- 102000007999 Nuclear Proteins Human genes 0.000 description 1
- 108010089610 Nuclear Proteins Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 102000004067 Osteocalcin Human genes 0.000 description 1
- 108090000573 Osteocalcin Proteins 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 101710104378 Putative malate oxidoreductase [NAD] Proteins 0.000 description 1
- 108091027981 Response element Proteins 0.000 description 1
- 108010068542 Somatotropin Receptors Proteins 0.000 description 1
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 description 1
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 description 1
- 108010051583 Ventricular Myosins Proteins 0.000 description 1
- XYIPYISRNJUPBA-UHFFFAOYSA-N [3-(3'-methoxyspiro[adamantane-2,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate Chemical compound O1OC2(C3CC4CC(C3)CC2C4)C1(OC)C1=CC=CC(OP(O)(O)=O)=C1 XYIPYISRNJUPBA-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000008484 agonism Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000007824 enzymatic assay Methods 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000002657 hormone replacement therapy Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 102000027411 intracellular receptors Human genes 0.000 description 1
- 108091008582 intracellular receptors Proteins 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 230000009245 menopause Effects 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 239000004031 partial agonist Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 102000003998 progesterone receptors Human genes 0.000 description 1
- 108090000468 progesterone receptors Proteins 0.000 description 1
- 230000001373 regressive effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000003153 stable transfection Methods 0.000 description 1
- 230000003637 steroidlike Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 229960005356 urokinase Drugs 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Toxicology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Endocrinology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Priority Applications (14)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9102901A SE500453C2 (sv) | 1991-10-07 | 1991-10-07 | Ett in vitro förfarande för utvärdering av en substans effekter |
| DE69221629T DE69221629T2 (de) | 1991-10-07 | 1992-10-06 | In vitro verfahren zur bewertung von effekten einer substanz |
| PCT/SE1992/000698 WO1993007290A1 (en) | 1991-10-07 | 1992-10-06 | An in vitro method of evaluating the effects of a substance |
| CA002120604A CA2120604C (en) | 1991-10-07 | 1992-10-06 | An in vitro method of evaluating the effects of a substance |
| ES92921468T ES2104945T3 (es) | 1991-10-07 | 1992-10-06 | Un metodo in vitro para evaluar los efectos de una sustancia. |
| EP92921468A EP0607268B1 (de) | 1991-10-07 | 1992-10-06 | In vitro verfahren zur bewertung von effekten einer substanz |
| AT92921468T ATE156862T1 (de) | 1991-10-07 | 1992-10-06 | In vitro verfahren zur bewertung von effekten einer substanz |
| AU27735/92A AU657171B2 (en) | 1991-10-07 | 1992-10-06 | An (in vitro) method of evaluating the effects of a substance |
| JP5506843A JP2698476B2 (ja) | 1991-10-07 | 1992-10-06 | 物質の効果を評価するインビトロ法 |
| DK92921468.2T DK0607268T3 (da) | 1991-10-07 | 1992-10-06 | In vitro fremgangsmåde til evaluering af virkningerne af et stof |
| US08/211,487 US5578445A (en) | 1991-10-07 | 1992-10-06 | In vitro method of evaluating the effects of a substance |
| NO19941246A NO310439B1 (no) | 1991-10-07 | 1994-04-06 | In vitro fremgangsmåte for evaluering av et stoffs virkninger |
| FI941611A FI103672B (fi) | 1991-10-07 | 1994-04-07 | Menetelmä aineen vaikutusten arvioimiseksi in vitro |
| GR970402995T GR3025354T3 (en) | 1991-10-07 | 1997-11-12 | AN -i(IN VITRO) METHOD OF EVALUATING THE EFFECTS OF A SUBSTANCE. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9102901A SE500453C2 (sv) | 1991-10-07 | 1991-10-07 | Ett in vitro förfarande för utvärdering av en substans effekter |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| SE9102901D0 SE9102901D0 (sv) | 1991-10-07 |
| SE9102901L SE9102901L (sv) | 1993-04-08 |
| SE500453C2 true SE500453C2 (sv) | 1994-06-27 |
Family
ID=20383927
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE9102901A SE500453C2 (sv) | 1991-10-07 | 1991-10-07 | Ett in vitro förfarande för utvärdering av en substans effekter |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US5578445A (de) |
| EP (1) | EP0607268B1 (de) |
| JP (1) | JP2698476B2 (de) |
| AT (1) | ATE156862T1 (de) |
| AU (1) | AU657171B2 (de) |
| CA (1) | CA2120604C (de) |
| DE (1) | DE69221629T2 (de) |
| DK (1) | DK0607268T3 (de) |
| ES (1) | ES2104945T3 (de) |
| FI (1) | FI103672B (de) |
| GR (1) | GR3025354T3 (de) |
| NO (1) | NO310439B1 (de) |
| SE (1) | SE500453C2 (de) |
| WO (1) | WO1993007290A1 (de) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5461065A (en) * | 1993-10-15 | 1995-10-24 | Eli Lilly And Company | Methods for inhibiting endometriosis |
| US5461064A (en) * | 1993-12-21 | 1995-10-24 | Eli Lilly And Company | Methods of inhibiting atrophy of the skin and vagina |
| US6417198B1 (en) * | 1993-12-21 | 2002-07-09 | Eli Lilly And Company | Methods of inhibiting CNS problems in post-menopausal women |
| US6516294B1 (en) | 1999-07-01 | 2003-02-04 | The Regents Of The University Of California | Nuclear receptor for 1α,25-dihydroxyvitamin D3 useful for selection of vitamin D3 ligands and a method therefor |
| US20020001797A1 (en) * | 2000-06-30 | 2002-01-03 | Mitsuko Ishihara | Method for detecting endocrine disrupting action of a test substance |
| US20080085912A1 (en) * | 2006-10-06 | 2008-04-10 | Unitech Pharmaceuticals, Inc. | Isoxazole derivatives and methods of treating diseases |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4859585A (en) * | 1986-04-17 | 1989-08-22 | Trustees Of Tufts College | In-vitro methods for identifying compositions which are agonists and antagonists of estrogens |
| US5071773A (en) * | 1986-10-24 | 1991-12-10 | The Salk Institute For Biological Studies | Hormone receptor-related bioassays |
| US5266464A (en) * | 1988-02-10 | 1993-11-30 | Ict Pharmaceuticals, Inc. | Method of screening for protein inhibitors and activators |
| US5091518A (en) * | 1989-11-16 | 1992-02-25 | The Salk Institute For Biological Studies | Beta retinoic acid response elements compositions and assays |
-
1991
- 1991-10-07 SE SE9102901A patent/SE500453C2/sv not_active IP Right Cessation
-
1992
- 1992-10-06 EP EP92921468A patent/EP0607268B1/de not_active Revoked
- 1992-10-06 AT AT92921468T patent/ATE156862T1/de not_active IP Right Cessation
- 1992-10-06 DE DE69221629T patent/DE69221629T2/de not_active Revoked
- 1992-10-06 JP JP5506843A patent/JP2698476B2/ja not_active Expired - Fee Related
- 1992-10-06 ES ES92921468T patent/ES2104945T3/es not_active Expired - Lifetime
- 1992-10-06 DK DK92921468.2T patent/DK0607268T3/da active
- 1992-10-06 US US08/211,487 patent/US5578445A/en not_active Expired - Fee Related
- 1992-10-06 AU AU27735/92A patent/AU657171B2/en not_active Ceased
- 1992-10-06 WO PCT/SE1992/000698 patent/WO1993007290A1/en not_active Ceased
- 1992-10-06 CA CA002120604A patent/CA2120604C/en not_active Expired - Fee Related
-
1994
- 1994-04-06 NO NO19941246A patent/NO310439B1/no not_active IP Right Cessation
- 1994-04-07 FI FI941611A patent/FI103672B/fi active
-
1997
- 1997-11-12 GR GR970402995T patent/GR3025354T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU657171B2 (en) | 1995-03-02 |
| CA2120604A1 (en) | 1993-04-15 |
| DK0607268T3 (da) | 1997-09-08 |
| EP0607268B1 (de) | 1997-08-13 |
| AU2773592A (en) | 1993-05-03 |
| ATE156862T1 (de) | 1997-08-15 |
| SE9102901L (sv) | 1993-04-08 |
| FI103672B1 (fi) | 1999-08-13 |
| FI941611L (fi) | 1994-04-07 |
| FI103672B (fi) | 1999-08-13 |
| EP0607268A1 (de) | 1994-07-27 |
| DE69221629D1 (de) | 1997-09-18 |
| NO941246D0 (no) | 1994-04-06 |
| ES2104945T3 (es) | 1997-10-16 |
| JP2698476B2 (ja) | 1998-01-19 |
| JPH07500184A (ja) | 1995-01-05 |
| DE69221629T2 (de) | 1998-01-22 |
| WO1993007290A1 (en) | 1993-04-15 |
| US5578445A (en) | 1996-11-26 |
| FI941611A0 (fi) | 1994-04-07 |
| NO310439B1 (no) | 2001-07-02 |
| GR3025354T3 (en) | 1998-02-27 |
| SE9102901D0 (sv) | 1991-10-07 |
| NO941246L (no) | 1994-04-06 |
| CA2120604C (en) | 1999-08-17 |
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