SG49863A1 - In vitro processing of fusion proteins - Google Patents

In vitro processing of fusion proteins

Info

Publication number: SG49863A1
Authority: SG; Singapore
Prior art keywords: protein; biologically active; amino acid; terminal amino; active protein
Prior art date: 1990-07-18

Application number

SG9607721A

Other languages

English (en)

Inventor

Jutta Dr Heim

Peter Dr Seeboth

Kenji Dr Takabayashi

Jutta Heim

Peter Seeboth

Kenji Takabayashi

Original Assignee

Novartis Ag

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-07-18

Filing date

1991-07-09

Publication date

2002-03-19

1991-07-09 Application filed by Novartis Ag filed Critical Novartis Ag

2002-03-19 Publication of SG49863A1 publication Critical patent/SG49863A1/en

Links

102000037865 fusion proteins Human genes 0.000 title abstract 2
108020001507 fusion proteins Proteins 0.000 title abstract 2
238000000338 in vitro Methods 0.000 title 1
108090000623 proteins and genes Proteins 0.000 abstract 7
102000004169 proteins and genes Human genes 0.000 abstract 7
229920001184 polypeptide Polymers 0.000 abstract 4
102000004196 processed proteins & peptides Human genes 0.000 abstract 4
108090000765 processed proteins & peptides Proteins 0.000 abstract 4
125000001433 C-terminal amino-acid group Chemical group 0.000 abstract 2
240000004808 Saccharomyces cerevisiae Species 0.000 abstract 2
125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 abstract 1
101710118538 Protease Proteins 0.000 abstract 1
101100053619 Yersinia enterocolitica yscF gene Proteins 0.000 abstract 1
108010067541 carboxypeptidase ysc-alpha Proteins 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/585—Calcitonins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Molecular Biology (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Medicinal Chemistry (AREA)
Biophysics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Gastroenterology & Hepatology (AREA)
Microbiology (AREA)
Toxicology (AREA)
Mycology (AREA)
Endocrinology (AREA)
Tropical Medicine & Parasitology (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Diabetes (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)

SG9607721A 1990-07-18 1991-07-09 In vitro processing of fusion proteins SG49863A1 (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
GB909015825A GB9015825D0 (en)	1990-07-18	1990-07-18	In vitro processing of fusion proteins

Publications (1)

Publication Number	Publication Date
SG49863A1 true SG49863A1 (en)	2002-03-19

Family

ID=10679295

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
SG9607721A SG49863A1 (en)	1990-07-18	1991-07-09	In vitro processing of fusion proteins

Country Status (19)

Country	Link
EP (1)	EP0467839B1 (de)
JP (1)	JP3140488B2 (de)
AT (1)	ATE141645T1 (de)
AU (1)	AU651830B2 (de)
CA (1)	CA2047119C (de)
DE (1)	DE69121489T2 (de)
DK (1)	DK0467839T3 (de)
ES (1)	ES2091897T3 (de)
FI (1)	FI106720B (de)
GB (1)	GB9015825D0 (de)
GR (1)	GR3020840T3 (de)
HU (1)	HU216104B (de)
IE (1)	IE73209B1 (de)
NO (1)	NO303132B1 (de)
NZ (1)	NZ238989A (de)
PT (1)	PT98332B (de)
SG (1)	SG49863A1 (de)
TW (1)	TW209247B (de)
ZA (1)	ZA915579B (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5525491A (en) *	1991-02-27	1996-06-11	Creative Biomolecules, Inc.	Serine-rich peptide linkers
WO1997023639A1 (en) *	1995-12-22	1997-07-03	Toray Industries, Inc.	Process for producing biologically active fused proteins
US6800726B1 (en)	1996-11-01	2004-10-05	Pioneer Hi-Bred International, Inc.	Proteins with increased levels of essential amino acids
US6265204B1 (en)	1997-01-17	2001-07-24	Genencor International, Inc.	DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
GB9716790D0 (en)	1997-08-07	1997-10-15	Creative Peptides Sweden Ab	Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof
CA2903739A1 (en)	2013-03-06	2014-09-12	Glaxosmithkline Llc	Host cells and methods of use
CN118515782B (zh) *	2024-06-03	2025-03-18	浙江毓昌生物技术有限公司	一种融合蛋白及基于该融合蛋白制备重组人生长激素的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0252854A1 (de) *	1986-07-10	1988-01-13	Transgene S.A.	Funktioneller DNS-Block und für Hirudin kodierendes Plasmid, transformierte Hefe aud Verfahren zur Herstellung von Hirudin.
EP0301669A1 (de) *	1987-07-28	1989-02-01	Gist-Brocades N.V.	DNS-Konstruktionen, enthaltend eine Kluyveromyces-alpha-Factor-Leadersequenz zur Leitung der Sekretion von heterologen Polypeptiden

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DK105489D0 (da) *	1989-03-03	1989-03-03	Novo Nordisk As	Polypeptid

1990
- 1990-07-18 GB GB909015825A patent/GB9015825D0/en active Pending
1991
- 1991-07-09 DE DE69121489T patent/DE69121489T2/de not_active Expired - Fee Related
- 1991-07-09 SG SG9607721A patent/SG49863A1/en unknown
- 1991-07-09 AT AT91810543T patent/ATE141645T1/de not_active IP Right Cessation
- 1991-07-09 ES ES91810543T patent/ES2091897T3/es not_active Expired - Lifetime
- 1991-07-09 DK DK91810543.8T patent/DK0467839T3/da active
- 1991-07-09 EP EP91810543A patent/EP0467839B1/de not_active Expired - Lifetime
- 1991-07-15 FI FI913405A patent/FI106720B/fi active
- 1991-07-16 AU AU80463/91A patent/AU651830B2/en not_active Ceased
- 1991-07-16 PT PT98332A patent/PT98332B/pt not_active IP Right Cessation
- 1991-07-16 CA CA002047119A patent/CA2047119C/en not_active Expired - Lifetime
- 1991-07-16 NZ NZ238989A patent/NZ238989A/en not_active IP Right Cessation
- 1991-07-17 HU HU2398/91A patent/HU216104B/hu not_active IP Right Cessation
- 1991-07-17 TW TW080105554A patent/TW209247B/zh active
- 1991-07-17 NO NO912803A patent/NO303132B1/no not_active IP Right Cessation
- 1991-07-17 ZA ZA915579A patent/ZA915579B/xx unknown
- 1991-07-17 JP JP03176552A patent/JP3140488B2/ja not_active Expired - Fee Related
- 1991-07-17 IE IE250191A patent/IE73209B1/en not_active IP Right Cessation
1996
- 1996-08-22 GR GR960402054T patent/GR3020840T3/el unknown

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0252854A1 (de) *	1986-07-10	1988-01-13	Transgene S.A.	Funktioneller DNS-Block und für Hirudin kodierendes Plasmid, transformierte Hefe aud Verfahren zur Herstellung von Hirudin.
EP0301669A1 (de) *	1987-07-28	1989-02-01	Gist-Brocades N.V.	DNS-Konstruktionen, enthaltend eine Kluyveromyces-alpha-Factor-Leadersequenz zur Leitung der Sekretion von heterologen Polypeptiden

Also Published As

Publication number	Publication date
GR3020840T3 (en)	1996-11-30
ZA915579B (en)	1992-03-25
NO303132B1 (no)	1998-06-02
EP0467839B1 (de)	1996-08-21
ATE141645T1 (de)	1996-09-15
PT98332B (pt)	1999-01-29
HU216104B (hu)	1999-04-28
FI913405A0 (fi)	1991-07-15
NO912803D0 (no)	1991-07-17
IE73209B1 (en)	1997-05-07
FI913405A7 (fi)	1992-01-19
JP3140488B2 (ja)	2001-03-05
DK0467839T3 (da)	1996-09-16
ES2091897T3 (es)	1996-11-16
TW209247B (de)	1993-07-11
CA2047119A1 (en)	1992-01-19
EP0467839A1 (de)	1992-01-22
NO912803L (no)	1992-01-20
AU8046391A (en)	1992-01-23
HUT58369A (en)	1992-02-28
GB9015825D0 (en)	1990-09-05
IE912501A1 (en)	1992-01-29
HU912398D0 (en)	1991-12-30
PT98332A (pt)	1992-05-29
DE69121489T2 (de)	1997-02-27
CA2047119C (en)	2002-10-08
DE69121489D1 (de)	1996-09-26
JPH04229188A (ja)	1992-08-18
NZ238989A (en)	1992-09-25
FI106720B (fi)	2001-03-30
AU651830B2 (en)	1994-08-04

Publication	Publication Date	Title
CA2050336A1 (en)	1990-09-04	Yeast processing system comprising a negatively charged amino acid adjacent to the processing site
ES2124685T3 (es)	1999-02-16	Variantes del inhibidor de la tripsina pancreatica bovina, su produccion y uso.
EP1225225A3 (de)	2003-01-08	Osteogene Vorrichtungen
GR3021106T3 (en)	1996-12-31	Production of bacteria and yeast of hemoglobin and analogues thereof
GR3033094T3 (en)	2000-08-31	Methods for trans-destabilization of specific proteins in vivo.
ZA8860B (en)	1988-10-26	Pancreatic secretory trypsin inhibitor and variants thereof produced by a recombinant host,process,expression vector and recombinant host therefor and pharmaceutical use thereof
DE3852706D1 (de)	1995-02-23	Verfahren zur Herstellung eines physiologisch aktiven Peptids mit einem Cysteinrest.
AU651830B2 (en)	1994-08-04	In vitro processing of fusion proteins
DE69233245D1 (de)	2003-12-11	Verfahren zur Herstellung von Peptiden
WO1999002708A8 (en)	1999-04-01	Fusion proteins comprising sequences derived from bovine if1 atpase inhibitor protein
IE830529L (en)	1983-09-13	Insulins from preproinsulins
DE59108261D1 (de)	1996-11-14	Peptide des HIV-gag Proteins, ihre Herstellung und Verwendung
AU4664489A (en)	1990-06-26	Ancrod proteins, their production and use
ATE110775T1 (de)	1994-09-15	Verfahren zur herstellung von hirudin.
DK53290A (da)	1990-03-01	Syntetiske gaer-leader-peptider