SK19892000A3 - Vektor propiónovej baktérie - Google Patents

Vektor propiónovej baktérie Download PDF

Info

Publication number: SK19892000A3
Authority: SK; Slovakia
Prior art keywords: host cell; vector; polypeptide; polynucleotide; plasmid
Prior art date: 1998-06-25

Application number

SK1989-2000A

Other languages

English (en)

Slovak (sk)

Inventor

Pieter Hendrik Pouwels

Luijk Nicole Van

Johannes Petrus Maria Jore

Rudolf Gijsbertus Marie Luiten

Original Assignee

Gist-Brocades B. V.

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1998-06-25

Filing date

1999-06-25

Publication date

2001-07-10

1999-06-25 Application filed by Gist-Brocades B. V. filed Critical Gist-Brocades B. V.

2001-07-10 Publication of SK19892000A3 publication Critical patent/SK19892000A3/sk

Links

241000186429 Propionibacterium Species 0.000 title claims abstract description 31
239000013598 vector Substances 0.000 title claims description 95
239000013612 plasmid Substances 0.000 claims abstract description 104
108090000623 proteins and genes Proteins 0.000 claims abstract description 87
238000004519 manufacturing process Methods 0.000 claims abstract description 29
FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 claims abstract 2
108091033319 polynucleotide Proteins 0.000 claims description 83
239000002157 polynucleotide Substances 0.000 claims description 83
102000040430 polynucleotide Human genes 0.000 claims description 83
108090000765 processed proteins & peptides Proteins 0.000 claims description 60
229920001184 polypeptide Polymers 0.000 claims description 59
102000004196 processed proteins & peptides Human genes 0.000 claims description 59
241000894006 Bacteria Species 0.000 claims description 50
238000000034 method Methods 0.000 claims description 41
239000012634 fragment Substances 0.000 claims description 40
241000588724 Escherichia coli Species 0.000 claims description 28
150000001875 compounds Chemical class 0.000 claims description 26
125000003729 nucleotide group Chemical group 0.000 claims description 22
241000186428 Propionibacterium freudenreichii Species 0.000 claims description 21
239000002773 nucleotide Substances 0.000 claims description 21
230000010076 replication Effects 0.000 claims description 20
235000013351 cheese Nutrition 0.000 claims description 16
230000014509 gene expression Effects 0.000 claims description 16
241001465754 Metazoa Species 0.000 claims description 12
125000003275 alpha amino acid group Chemical group 0.000 claims description 10
238000000855 fermentation Methods 0.000 claims description 9
230000004151 fermentation Effects 0.000 claims description 9
229930003231 vitamin Natural products 0.000 claims description 9
235000013343 vitamin Nutrition 0.000 claims description 9
239000011782 vitamin Substances 0.000 claims description 9
229940088594 vitamin Drugs 0.000 claims description 9
101150087945 cobA gene Proteins 0.000 claims description 8
229930003270 Vitamin B Natural products 0.000 claims description 7
238000012258 culturing Methods 0.000 claims description 7
238000009396 hybridization Methods 0.000 claims description 7
235000019156 vitamin B Nutrition 0.000 claims description 7
239000011720 vitamin B Substances 0.000 claims description 7
239000000427 antigen Substances 0.000 claims description 6
108091007433 antigens Proteins 0.000 claims description 6
102000036639 antigens Human genes 0.000 claims description 6
235000013305 food Nutrition 0.000 claims description 6
239000003550 marker Substances 0.000 claims description 6
230000003362 replicative effect Effects 0.000 claims description 6
150000003722 vitamin derivatives Chemical class 0.000 claims description 6
230000001225 therapeutic effect Effects 0.000 claims description 5
241000282414 Homo sapiens Species 0.000 claims description 4
108090001060 Lipase Proteins 0.000 claims description 4
108091005804 Peptidases Proteins 0.000 claims description 4
239000013604 expression vector Substances 0.000 claims description 4
JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 4
241000282412 Homo Species 0.000 claims description 3
102000004882 Lipase Human genes 0.000 claims description 3
239000004367 Lipase Substances 0.000 claims description 3
102000035195 Peptidases Human genes 0.000 claims description 3
241001237745 Salamis Species 0.000 claims description 3
235000019421 lipase Nutrition 0.000 claims description 3
230000008569 process Effects 0.000 claims description 3
235000015175 salami Nutrition 0.000 claims description 3
239000013589 supplement Substances 0.000 claims description 3
230000001131 transforming effect Effects 0.000 claims description 3
102000013142 Amylases Human genes 0.000 claims description 2
108010065511 Amylases Proteins 0.000 claims description 2
239000004365 Protease Substances 0.000 claims description 2
235000019418 amylase Nutrition 0.000 claims description 2
235000021001 fermented dairy product Nutrition 0.000 claims description 2
230000002163 immunogen Effects 0.000 claims description 2
239000004310 lactic acid Substances 0.000 claims description 2
235000014655 lactic acid Nutrition 0.000 claims description 2
235000019833 protease Nutrition 0.000 claims description 2
JZRWCGZRTZMZEH-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims description 2
239000004382 Amylase Substances 0.000 claims 1
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
235000019419 proteases Nutrition 0.000 claims 1
102000004169 proteins and genes Human genes 0.000 abstract description 28
102000004190 Enzymes Human genes 0.000 abstract description 10
108090000790 Enzymes Proteins 0.000 abstract description 10
102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract description 2
108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract description 2
229930003779 Vitamin B12 Natural products 0.000 abstract 1
239000011715 vitamin B12 Substances 0.000 abstract 1
235000019163 vitamin B12 Nutrition 0.000 abstract 1
210000004027 cell Anatomy 0.000 description 69
108020004414 DNA Proteins 0.000 description 40
ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 30
239000013615 primer Substances 0.000 description 19
108091008146 restriction endonucleases Proteins 0.000 description 17
230000009466 transformation Effects 0.000 description 16
229960003276 erythromycin Drugs 0.000 description 15
108091026890 Coding region Proteins 0.000 description 14
150000001413 amino acids Chemical class 0.000 description 14
238000003752 polymerase chain reaction Methods 0.000 description 10
238000006467 substitution reaction Methods 0.000 description 10
229940088598 enzyme Drugs 0.000 description 9
229960005091 chloramphenicol Drugs 0.000 description 8
WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 8
238000010367 cloning Methods 0.000 description 8
239000002609 medium Substances 0.000 description 8
239000000523 sample Substances 0.000 description 8
241000186361 Actinobacteria <class> Species 0.000 description 7
238000010276 construction Methods 0.000 description 7
239000000203 mixture Substances 0.000 description 7
244000058084 Aegle marmelos Species 0.000 description 6
235000003930 Aegle marmelos Nutrition 0.000 description 6
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
239000005720 sucrose Substances 0.000 description 6
108091028043 Nucleic acid sequence Proteins 0.000 description 5
229930006000 Sucrose Natural products 0.000 description 5
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 5
238000004458 analytical method Methods 0.000 description 5
238000002869 basic local alignment search tool Methods 0.000 description 5
230000000694 effects Effects 0.000 description 5
238000003780 insertion Methods 0.000 description 5
230000037431 insertion Effects 0.000 description 5
239000002987 primer (paints) Substances 0.000 description 5
239000000047 product Substances 0.000 description 5
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
241000186426 Acidipropionibacterium acidipropionici Species 0.000 description 4
241000192125 Firmicutes Species 0.000 description 4
102000003960 Ligases Human genes 0.000 description 4
108090000364 Ligases Proteins 0.000 description 4
230000000295 complement effect Effects 0.000 description 4
238000004520 electroporation Methods 0.000 description 4
150000007523 nucleic acids Chemical group 0.000 description 4
239000013600 plasmid vector Substances 0.000 description 4
229960005486 vaccine Drugs 0.000 description 4
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 3
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
241000186425 Acidipropionibacterium jensenii Species 0.000 description 3
229920001817 Agar Polymers 0.000 description 3
101150001086 COB gene Proteins 0.000 description 3
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
108091034117 Oligonucleotide Proteins 0.000 description 3
101710150114 Protein rep Proteins 0.000 description 3
101710152114 Replication protein Proteins 0.000 description 3
AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 3
241000187559 Saccharopolyspora erythraea Species 0.000 description 3
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
239000008272 agar Substances 0.000 description 3
101150098767 btuR gene Proteins 0.000 description 3
229910052799 carbon Inorganic materials 0.000 description 3
ASARMUCNOOHMLO-WLORSUFZSA-L cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2s)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@H](C)OP([O-])(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O ASARMUCNOOHMLO-WLORSUFZSA-L 0.000 description 3
238000012217 deletion Methods 0.000 description 3
230000037430 deletion Effects 0.000 description 3
230000029087 digestion Effects 0.000 description 3
238000009585 enzyme analysis Methods 0.000 description 3
238000011534 incubation Methods 0.000 description 3
230000003993 interaction Effects 0.000 description 3
238000002955 isolation Methods 0.000 description 3
238000012986 modification Methods 0.000 description 3
230000004048 modification Effects 0.000 description 3
229910052757 nitrogen Inorganic materials 0.000 description 3
102000039446 nucleic acids Human genes 0.000 description 3
108020004707 nucleic acids Proteins 0.000 description 3
239000011780 sodium chloride Substances 0.000 description 3
239000000126 substance Substances 0.000 description 3
239000000758 substrate Substances 0.000 description 3
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
241000432536 Acidipropionibacterium acidipropionici ATCC 4875 Species 0.000 description 2
241000186335 Acidipropionibacterium thoenii Species 0.000 description 2
CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
108020004705 Codon Proteins 0.000 description 2
241000186226 Corynebacterium glutamicum Species 0.000 description 2
241000186427 Cutibacterium acnes Species 0.000 description 2
238000007399 DNA isolation Methods 0.000 description 2
241000620209 Escherichia coli DH5[alpha] Species 0.000 description 2
108700039691 Genetic Promoter Regions Proteins 0.000 description 2
101100027898 Homo sapiens OCR1 gene Proteins 0.000 description 2
KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
108700026244 Open Reading Frames Proteins 0.000 description 2
102100026725 Ovarian cancer-related protein 1 Human genes 0.000 description 2
101710192394 Putative replication protein Proteins 0.000 description 2
108020005091 Replication Origin Proteins 0.000 description 2
241000634742 Saccharopolyspora erythraea NRRL 2338 Species 0.000 description 2
238000012300 Sequence Analysis Methods 0.000 description 2
238000002105 Southern blotting Methods 0.000 description 2
239000004098 Tetracycline Substances 0.000 description 2
UYXTWWCETRIEDR-UHFFFAOYSA-N Tributyrin Chemical compound CCCC(=O)OCC(OC(=O)CCC)COC(=O)CCC UYXTWWCETRIEDR-UHFFFAOYSA-N 0.000 description 2
DZBUGLKDJFMEHC-UHFFFAOYSA-N acridine Chemical group C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 2
230000003321 amplification Effects 0.000 description 2
238000003556 assay Methods 0.000 description 2
230000001580 bacterial effect Effects 0.000 description 2
229960002685 biotin Drugs 0.000 description 2
235000020958 biotin Nutrition 0.000 description 2
239000011616 biotin Substances 0.000 description 2
210000004556 brain Anatomy 0.000 description 2
229940041514 candida albicans extract Drugs 0.000 description 2
239000006285 cell suspension Substances 0.000 description 2
238000006243 chemical reaction Methods 0.000 description 2
239000003795 chemical substances by application Substances 0.000 description 2
239000013599 cloning vector Substances 0.000 description 2
238000001514 detection method Methods 0.000 description 2
239000003085 diluting agent Substances 0.000 description 2
XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
239000003814 drug Substances 0.000 description 2
239000003937 drug carrier Substances 0.000 description 2
229960005542 ethidium bromide Drugs 0.000 description 2
238000002474 experimental method Methods 0.000 description 2
239000000284 extract Substances 0.000 description 2
238000001802 infusion Methods 0.000 description 2
238000002372 labelling Methods 0.000 description 2
238000013507 mapping Methods 0.000 description 2
235000013336 milk Nutrition 0.000 description 2
239000008267 milk Substances 0.000 description 2
210000004080 milk Anatomy 0.000 description 2
238000003199 nucleic acid amplification method Methods 0.000 description 2
244000052769 pathogen Species 0.000 description 2
230000037361 pathway Effects 0.000 description 2
SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
238000002360 preparation method Methods 0.000 description 2
230000002250 progressing effect Effects 0.000 description 2
230000002285 radioactive effect Effects 0.000 description 2
230000001105 regulatory effect Effects 0.000 description 2
230000000717 retained effect Effects 0.000 description 2
230000002441 reversible effect Effects 0.000 description 2
238000012216 screening Methods 0.000 description 2
239000001509 sodium citrate Substances 0.000 description 2
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
229960002180 tetracycline Drugs 0.000 description 2
229930101283 tetracycline Natural products 0.000 description 2
235000019364 tetracycline Nutrition 0.000 description 2
150000003522 tetracyclines Chemical class 0.000 description 2
238000013519 translation Methods 0.000 description 2
239000012138 yeast extract Substances 0.000 description 2
CJVFIJXEPCVCTK-LWMBYGOMSA-N (2s)-2-[[2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-2-amino-3-methylbutanoyl]amino]propanoyl]amino]-3-methylbutanoyl]amino]-4-methylpentanoyl]amino]-3-methylbutanoyl]amino]-4-methylpentanoyl]amino]acetyl]amino]propanoic acid Chemical compound OC(=O)[C@H](C)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@@H](N)C(C)C)C(C)C CJVFIJXEPCVCTK-LWMBYGOMSA-N 0.000 description 1
108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 description 1
QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
101100301559 Bacillus anthracis repS gene Proteins 0.000 description 1
108010006654 Bleomycin Proteins 0.000 description 1
241001112696 Clostridia Species 0.000 description 1
101100247969 Clostridium saccharobutylicum regA gene Proteins 0.000 description 1
108091035707 Consensus sequence Proteins 0.000 description 1
241000186216 Corynebacterium Species 0.000 description 1
102000053602 DNA Human genes 0.000 description 1
108020001019 DNA Primers Proteins 0.000 description 1
239000003155 DNA primer Substances 0.000 description 1
QSJXEFYPDANLFS-UHFFFAOYSA-N Diacetyl Chemical group CC(=O)C(C)=O QSJXEFYPDANLFS-UHFFFAOYSA-N 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
101100412434 Escherichia coli (strain K12) repB gene Proteins 0.000 description 1
101100184605 Escherichia coli mobA gene Proteins 0.000 description 1
108050001049 Extracellular proteins Proteins 0.000 description 1
241000233866 Fungi Species 0.000 description 1
230000005526 G1 to G0 transition Effects 0.000 description 1
108700007698 Genetic Terminator Regions Proteins 0.000 description 1
241000447437 Gerreidae Species 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
102000016397 Methyltransferase Human genes 0.000 description 1
108060004795 Methyltransferase Proteins 0.000 description 1
102000016943 Muramidase Human genes 0.000 description 1
108010014251 Muramidase Proteins 0.000 description 1
241000186359 Mycobacterium Species 0.000 description 1
241000186365 Mycobacterium fortuitum Species 0.000 description 1
108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
229930193140 Neomycin Natural products 0.000 description 1
108010053775 Nisin Proteins 0.000 description 1
NVNLLIYOARQCIX-MSHCCFNRSA-N Nisin Chemical compound N1C(=O)[C@@H](CC(C)C)NC(=O)C(=C)NC(=O)[C@@H]([C@H](C)CC)NC(=O)[C@@H](NC(=O)C(=C/C)/NC(=O)[C@H](N)[C@H](C)CC)CSC[C@@H]1C(=O)N[C@@H]1C(=O)N2CCC[C@@H]2C(=O)NCC(=O)N[C@@H](C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(NCC(=O)N[C@H](C)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCSC)C(=O)NCC(=O)N[C@H](CS[C@@H]2C)C(=O)N[C@H](CC(N)=O)C(=O)N[C@H](CCSC)C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(N[C@H](C)C(=O)N[C@@H]3C(=O)N[C@@H](C(N[C@H](CC=4NC=NC=4)C(=O)N[C@H](CS[C@@H]3C)C(=O)N[C@H](CO)C(=O)N[C@H]([C@H](C)CC)C(=O)N[C@H](CC=3NC=NC=3)C(=O)N[C@H](C(C)C)C(=O)NC(=C)C(=O)N[C@H](CCCCN)C(O)=O)=O)CS[C@@H]2C)=O)=O)CS[C@@H]1C NVNLLIYOARQCIX-MSHCCFNRSA-N 0.000 description 1
101100135121 Oryza sativa subsp. japonica RR21 gene Proteins 0.000 description 1
101100135123 Oryza sativa subsp. japonica RR22 gene Proteins 0.000 description 1
102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
108010039918 Polylysine Chemical group 0.000 description 1
102000055027 Protein Methyltransferases Human genes 0.000 description 1
108700040121 Protein Methyltransferases Proteins 0.000 description 1
101100391699 Pseudomonas viridiflava gacA gene Proteins 0.000 description 1
241001417518 Rachycentridae Species 0.000 description 1
241000220317 Rosa Species 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
108050007496 Shikimate kinase 2 Proteins 0.000 description 1
101100114425 Streptococcus agalactiae copG gene Proteins 0.000 description 1
108700005078 Synthetic Genes Proteins 0.000 description 1
RYYWUUFWQRZTIU-UHFFFAOYSA-N Thiophosphoric acid Chemical group OP(O)(S)=O RYYWUUFWQRZTIU-UHFFFAOYSA-N 0.000 description 1
NSFFHOGKXHRQEW-UHFFFAOYSA-N Thiostrepton B Natural products N1C(=O)C(C)NC(=O)C(=C)NC(=O)C(C)NC(=O)C(C(C)CC)NC(C(C2=N3)O)C=CC2=C(C(C)O)C=C3C(=O)OC(C)C(C=2SC=C(N=2)C2N=3)NC(=O)C(N=4)=CSC=4C(C(C)(O)C(C)O)NC(=O)C(N=4)CSC=4C(=CC)NC(=O)C(C(C)O)NC(=O)C(N=4)=CSC=4C21CCC=3C1=NC(C(=O)NC(=C)C(=O)NC(=C)C(N)=O)=CS1 NSFFHOGKXHRQEW-UHFFFAOYSA-N 0.000 description 1
OZKXLOZHHUHGNV-UHFFFAOYSA-N Viomycin Natural products NCCCC(N)CC(=O)NC1CNC(=O)C(=CNC(=O)N)NC(=O)C(CO)NC(=O)C(CO)NC(=O)C(NC1=O)C2CC(O)NC(=N)N2 OZKXLOZHHUHGNV-UHFFFAOYSA-N 0.000 description 1
108010015940 Viomycin Proteins 0.000 description 1
241000700605 Viruses Species 0.000 description 1
229930003451 Vitamin B1 Natural products 0.000 description 1
230000002411 adverse Effects 0.000 description 1
239000011543 agarose gel Substances 0.000 description 1
238000000246 agarose gel electrophoresis Methods 0.000 description 1
125000001931 aliphatic group Chemical group 0.000 description 1
-1 ammonium ions Chemical class 0.000 description 1
229960000723 ampicillin Drugs 0.000 description 1
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
229940025131 amylases Drugs 0.000 description 1
239000003242 anti bacterial agent Substances 0.000 description 1
229940088710 antibiotic agent Drugs 0.000 description 1
230000000890 antigenic effect Effects 0.000 description 1
239000004599 antimicrobial Substances 0.000 description 1
229950006334 apramycin Drugs 0.000 description 1
XZNUGFQTQHRASN-XQENGBIVSA-N apramycin Chemical compound O([C@H]1O[C@@H]2[C@H](O)[C@@H]([C@H](O[C@H]2C[C@H]1N)O[C@@H]1[C@@H]([C@@H](O)[C@H](N)[C@@H](CO)O1)O)NC)[C@@H]1[C@@H](N)C[C@@H](N)[C@H](O)[C@H]1O XZNUGFQTQHRASN-XQENGBIVSA-N 0.000 description 1
239000012736 aqueous medium Substances 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
230000001588 bifunctional effect Effects 0.000 description 1
230000003115 biocidal effect Effects 0.000 description 1
230000001851 biosynthetic effect Effects 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
229960001561 bleomycin Drugs 0.000 description 1
OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
210000004899 c-terminal region Anatomy 0.000 description 1
238000004422 calculation algorithm Methods 0.000 description 1
238000004364 calculation method Methods 0.000 description 1
244000309466 calf Species 0.000 description 1
239000001569 carbon dioxide Substances 0.000 description 1
229910002092 carbon dioxide Inorganic materials 0.000 description 1
239000000969 carrier Substances 0.000 description 1
238000012512 characterization method Methods 0.000 description 1
239000013611 chromosomal DNA Substances 0.000 description 1
230000002759 chromosomal effect Effects 0.000 description 1
210000000349 chromosome Anatomy 0.000 description 1
238000012411 cloning technique Methods 0.000 description 1
101150100393 cml gene Proteins 0.000 description 1
FDJOLVPMNUYSCM-UVKKECPRSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2,7, Chemical compound [Co+3].N#[C-].C1([C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)[N-]\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O FDJOLVPMNUYSCM-UVKKECPRSA-L 0.000 description 1
230000009089 cytolysis Effects 0.000 description 1
235000013365 dairy product Nutrition 0.000 description 1
230000003412 degenerative effect Effects 0.000 description 1
239000012895 dilution Substances 0.000 description 1
238000010790 dilution Methods 0.000 description 1
229940079593 drug Drugs 0.000 description 1
239000003623 enhancer Substances 0.000 description 1
238000001976 enzyme digestion Methods 0.000 description 1
101150076810 erm gene Proteins 0.000 description 1
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
238000000605 extraction Methods 0.000 description 1
239000000796 flavoring agent Substances 0.000 description 1
239000007850 fluorescent dye Substances 0.000 description 1
235000012041 food component Nutrition 0.000 description 1
235000013355 food flavoring agent Nutrition 0.000 description 1
239000005417 food ingredient Substances 0.000 description 1
238000010230 functional analysis Methods 0.000 description 1
238000001502 gel electrophoresis Methods 0.000 description 1
108091008053 gene clusters Proteins 0.000 description 1
238000010353 genetic engineering Methods 0.000 description 1
239000008103 glucose Substances 0.000 description 1
125000001475 halogen functional group Chemical group 0.000 description 1
230000035876 healing Effects 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
238000004128 high performance liquid chromatography Methods 0.000 description 1
229940088597 hormone Drugs 0.000 description 1
239000005556 hormone Substances 0.000 description 1
229930195733 hydrocarbon Natural products 0.000 description 1
150000002430 hydrocarbons Chemical class 0.000 description 1
230000028993 immune response Effects 0.000 description 1
239000002955 immunomodulating agent Substances 0.000 description 1
229940121354 immunomodulator Drugs 0.000 description 1
238000001727 in vivo Methods 0.000 description 1
239000003999 initiator Substances 0.000 description 1
239000007924 injection Substances 0.000 description 1
238000002347 injection Methods 0.000 description 1
230000010354 integration Effects 0.000 description 1
230000002452 interceptive effect Effects 0.000 description 1
230000000968 intestinal effect Effects 0.000 description 1
238000007918 intramuscular administration Methods 0.000 description 1
238000001990 intravenous administration Methods 0.000 description 1
239000000644 isotonic solution Substances 0.000 description 1
229960000318 kanamycin Drugs 0.000 description 1
229930027917 kanamycin Natural products 0.000 description 1
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
229930182823 kanamycin A Natural products 0.000 description 1
229940001447 lactate Drugs 0.000 description 1
230000000670 limiting effect Effects 0.000 description 1
239000007788 liquid Substances 0.000 description 1
239000004325 lysozyme Substances 0.000 description 1
235000010335 lysozyme Nutrition 0.000 description 1
229960000274 lysozyme Drugs 0.000 description 1
239000000463 material Substances 0.000 description 1
230000002503 metabolic effect Effects 0.000 description 1
YACKEPLHDIMKIO-UHFFFAOYSA-N methylphosphonic acid Chemical compound CP(O)(O)=O YACKEPLHDIMKIO-UHFFFAOYSA-N 0.000 description 1
244000005700 microbiome Species 0.000 description 1
238000002156 mixing Methods 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
101150026150 mt gene Proteins 0.000 description 1
229960004927 neomycin Drugs 0.000 description 1
239000004309 nisin Substances 0.000 description 1
235000010297 nisin Nutrition 0.000 description 1
150000002823 nitrates Chemical class 0.000 description 1
235000016709 nutrition Nutrition 0.000 description 1
230000035764 nutrition Effects 0.000 description 1
235000003170 nutritional factors Nutrition 0.000 description 1
150000002894 organic compounds Chemical class 0.000 description 1
230000020477 pH reduction Effects 0.000 description 1
230000001717 pathogenic effect Effects 0.000 description 1
239000002504 physiological saline solution Substances 0.000 description 1
229920000656 polylysine Chemical group 0.000 description 1
235000011056 potassium acetate Nutrition 0.000 description 1
238000012545 processing Methods 0.000 description 1
239000013587 production medium Substances 0.000 description 1
235000019260 propionic acid Nutrition 0.000 description 1
IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
238000000163 radioactive labelling Methods 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
101150044854 repA gene Proteins 0.000 description 1
101150055347 repA2 gene Proteins 0.000 description 1
101150107738 repB gene Proteins 0.000 description 1
108020004418 ribosomal RNA Proteins 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
235000013580 sausages Nutrition 0.000 description 1
238000002864 sequence alignment Methods 0.000 description 1
238000002741 site-directed mutagenesis Methods 0.000 description 1
239000001540 sodium lactate Substances 0.000 description 1
229940005581 sodium lactate Drugs 0.000 description 1
235000011088 sodium lactate Nutrition 0.000 description 1
NGSFWBMYFKHRBD-DKWTVANSSA-M sodium;(2s)-2-hydroxypropanoate Chemical compound [Na+].C[C@H](O)C([O-])=O NGSFWBMYFKHRBD-DKWTVANSSA-M 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
239000008174 sterile solution Substances 0.000 description 1
229960005322 streptomycin Drugs 0.000 description 1
238000007920 subcutaneous administration Methods 0.000 description 1
238000003786 synthesis reaction Methods 0.000 description 1
238000010189 synthetic method Methods 0.000 description 1
229940124597 therapeutic agent Drugs 0.000 description 1
229960003495 thiamine Drugs 0.000 description 1
DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
229940063214 thiostrepton Drugs 0.000 description 1
229930188070 thiostrepton Natural products 0.000 description 1
NSFFHOGKXHRQEW-AIHSUZKVSA-N thiostrepton Chemical compound C([C@]12C=3SC=C(N=3)C(=O)N[C@H](C(=O)NC(/C=3SC[C@@H](N=3)C(=O)N[C@H](C=3SC=C(N=3)C(=O)N[C@H](C=3SC=C(N=3)[C@H]1N=1)[C@@H](C)OC(=O)C3=CC(=C4C=C[C@H]([C@@H](C4=N3)O)N[C@H](C(N[C@@H](C)C(=O)NC(=C)C(=O)N[C@@H](C)C(=O)N2)=O)[C@@H](C)CC)[C@H](C)O)[C@](C)(O)[C@@H](C)O)=C\C)[C@@H](C)O)CC=1C1=NC(C(=O)NC(=C)C(=O)NC(=C)C(N)=O)=CS1 NSFFHOGKXHRQEW-AIHSUZKVSA-N 0.000 description 1
NSFFHOGKXHRQEW-OFMUQYBVSA-N thiostrepton A Natural products CC[C@H](C)[C@@H]1N[C@@H]2C=Cc3c(cc(nc3[C@H]2O)C(=O)O[C@H](C)[C@@H]4NC(=O)c5csc(n5)[C@@H](NC(=O)[C@H]6CSC(=N6)C(=CC)NC(=O)[C@@H](NC(=O)c7csc(n7)[C@]8(CCC(=N[C@@H]8c9csc4n9)c%10nc(cs%10)C(=O)NC(=C)C(=O)NC(=C)C(=O)N)NC(=O)[C@H](C)NC(=O)C(=C)NC(=O)[C@H](C)NC1=O)[C@@H](C)O)[C@](C)(O)[C@@H](C)O)[C@H](C)O NSFFHOGKXHRQEW-OFMUQYBVSA-N 0.000 description 1
230000000699 topical effect Effects 0.000 description 1
238000013518 transcription Methods 0.000 description 1
230000005026 transcription initiation Effects 0.000 description 1
230000014621 translational initiation Effects 0.000 description 1
238000005199 ultracentrifugation Methods 0.000 description 1
238000011144 upstream manufacturing Methods 0.000 description 1
HUHWZXWWOFSFKF-UHFFFAOYSA-N uroporphyrinogen-III Chemical compound C1C(=C(C=2CCC(O)=O)CC(O)=O)NC=2CC(=C(C=2CCC(O)=O)CC(O)=O)NC=2CC(N2)=C(CC(O)=O)C(CCC(=O)O)=C2CC2=C(CCC(O)=O)C(CC(O)=O)=C1N2 HUHWZXWWOFSFKF-UHFFFAOYSA-N 0.000 description 1
239000003981 vehicle Substances 0.000 description 1
230000035899 viability Effects 0.000 description 1
229950001272 viomycin Drugs 0.000 description 1
GXFAIFRPOKBQRV-GHXCTMGLSA-N viomycin Chemical compound N1C(=O)\C(=C\NC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)C[C@@H](N)CCCN)CNC(=O)[C@@H]1[C@@H]1NC(=N)N[C@@H](O)C1 GXFAIFRPOKBQRV-GHXCTMGLSA-N 0.000 description 1
235000010374 vitamin B1 Nutrition 0.000 description 1
239000011691 vitamin B1 Substances 0.000 description 1
239000008215 water for injection Substances 0.000 description 1
235000013618 yogurt Nutrition 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)

Landscapes

Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Wood Science & Technology (AREA)
Organic Chemistry (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Physics & Mathematics (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)
Peptides Or Proteins (AREA)
Fodder In General (AREA)
Dairy Products (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Detergent Compositions (AREA)

SK1989-2000A 1998-06-25 1999-06-25 Vektor propiónovej baktérie SK19892000A3 (sk)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
EP98305033		1998-06-25
PCT/EP1999/004416 WO1999067356A2 (en)	1998-06-25	1999-06-25	Propionibacterium vector

Publications (1)

Publication Number	Publication Date
SK19892000A3 true SK19892000A3 (sk)	2001-07-10

Family

ID=8234893

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
SK1989-2000A SK19892000A3 (sk)	1998-06-25	1999-06-25	Vektor propiónovej baktérie

Country Status (24)

Country	Link
US (1)	US6830901B1 (cs)
EP (1)	EP1090120A2 (cs)
JP (1)	JP2002518039A (cs)
KR (1)	KR20010053141A (cs)
CN (1)	CN1314941A (cs)
AU (1)	AU4615899A (cs)
BG (1)	BG105078A (cs)
BR (1)	BR9911511A (cs)
CA (1)	CA2331924A1 (cs)
CZ (1)	CZ20004842A3 (cs)
FR (1)	FR2780733A1 (cs)
HR (1)	HRP20010017A2 (cs)
HU (1)	HUP0102820A3 (cs)
ID (1)	ID28046A (cs)
IL (1)	IL140461A0 (cs)
IS (1)	IS5783A (cs)
NL (1)	NL1012434C2 (cs)
NO (1)	NO20006616L (cs)
PL (1)	PL345519A1 (cs)
SK (1)	SK19892000A3 (cs)
TR (1)	TR200100351T2 (cs)
WO (1)	WO1999067356A2 (cs)
YU (1)	YU82600A (cs)
ZA (1)	ZA200007786B (cs)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2002518039A (ja)	1998-06-25	2002-06-25	デーエスエム・ナムローゼ・フェンノートシャップ	プロピオニバクテリウムベクター
US20040235120A1 (en) *	2001-08-22	2004-11-25	Andreas Kunkel	Method for producing vitamin b12
TWI328612B (en)	2002-07-25	2010-08-11	Dsm Ip Assets Bv	Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
EP1543026B1 (en) *	2002-09-27	2006-08-02	DSM IP Assets B.V.	Transcriptional activator gene for genes involved in cobalamin biosynthesis
KR101489723B1 (ko) *	2008-03-14	2015-02-06	인하대학교 산학협력단	전기천공법을 이용한 프로피오니박테리움 아크네스의형질전환 조건의 최적화
WO2011029166A1 (en) *	2009-09-09	2011-03-17	Braskem S.A.	Microorganisms and process for producing n-propanol
CN118979001A (zh)	2014-06-17	2024-11-19	皇冠实验室有限公司	经遗传修饰的细菌和用于细菌的遗传修饰的方法
JP2019503688A (ja) *	2016-01-11	2019-02-14	３ピーエルダブリューリミテッド	多糖分解酵素を分泌するように遺伝子改変された乳酸利用細菌
US11504404B2 (en)	2016-02-24	2022-11-22	Crown Laboratories, Inc.	Skin probiotic formulation
AU2021297215B2 (en)	2020-06-23	2026-08-13	Crown Laboratories, Inc.	Probiotic skin formulations

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPH02312590A (ja)	1989-05-29	1990-12-27	Nippon Oil Co Ltd	プラスミドｐＴＹ１
EP0647717A1 (en) *	1993-10-06	1995-04-12	Takeda Chemical Industries, Ltd.	Method of producing vitamin B12
JPH0856673A (ja)	1994-08-29	1996-03-05	Nippon Oil Co Ltd	ウロポルフィリノーゲンｉｉｉメチルトランスフェラーゼをコードするｄｎａ及びこれを用いるビタミンｂ１２の生産方法
JP2002518039A (ja)	1998-06-25	2002-06-25	デーエスエム・ナムローゼ・フェンノートシャップ	プロピオニバクテリウムベクター

1999
- 1999-06-25 JP JP2000556001A patent/JP2002518039A/ja not_active Withdrawn
- 1999-06-25 WO PCT/EP1999/004416 patent/WO1999067356A2/en not_active Ceased
- 1999-06-25 HU HU0102820A patent/HUP0102820A3/hu unknown
- 1999-06-25 US US09/720,583 patent/US6830901B1/en not_active Expired - Fee Related
- 1999-06-25 KR KR1020007014688A patent/KR20010053141A/ko not_active Withdrawn
- 1999-06-25 SK SK1989-2000A patent/SK19892000A3/sk unknown
- 1999-06-25 FR FR9908141A patent/FR2780733A1/fr not_active Withdrawn
- 1999-06-25 PL PL99345519A patent/PL345519A1/xx not_active Application Discontinuation
- 1999-06-25 BR BR9911511-5A patent/BR9911511A/pt not_active Application Discontinuation
- 1999-06-25 NL NL1012434A patent/NL1012434C2/nl not_active IP Right Cessation
- 1999-06-25 HR HR20010017A patent/HRP20010017A2/hr not_active Application Discontinuation
- 1999-06-25 EP EP99929316A patent/EP1090120A2/en not_active Withdrawn
- 1999-06-25 CA CA002331924A patent/CA2331924A1/en not_active Abandoned
- 1999-06-25 TR TR2001/00351T patent/TR200100351T2/xx unknown
- 1999-06-25 ID IDW20002742A patent/ID28046A/id unknown
- 1999-06-25 YU YU82600A patent/YU82600A/sh unknown
- 1999-06-25 IL IL14046199A patent/IL140461A0/xx unknown
- 1999-06-25 CZ CZ20004842A patent/CZ20004842A3/cs unknown
- 1999-06-25 AU AU46158/99A patent/AU4615899A/en not_active Abandoned
- 1999-06-25 CN CN99809489A patent/CN1314941A/zh active Pending
2000
- 2000-12-21 IS IS5783A patent/IS5783A/is unknown
- 2000-12-21 ZA ZA200007786A patent/ZA200007786B/en unknown
- 2000-12-21 BG BG105078A patent/BG105078A/xx unknown
- 2000-12-22 NO NO20006616A patent/NO20006616L/no not_active Application Discontinuation

Also Published As

Publication number	Publication date
WO1999067356A3 (en)	2000-02-10
FR2780733A1 (fr)	2000-01-07
TR200100351T2 (tr)	2001-07-23
CA2331924A1 (en)	1999-12-29
US6830901B1 (en)	2004-12-14
CN1314941A (zh)	2001-09-26
AU4615899A (en)	2000-01-10
NO20006616D0 (no)	2000-12-22
JP2002518039A (ja)	2002-06-25
EP1090120A2 (en)	2001-04-11
NO20006616L (no)	2001-02-26
CZ20004842A3 (cs)	2001-11-14
YU82600A (sh)	2004-03-12
BG105078A (en)	2001-09-28
HRP20010017A2 (en)	2001-12-31
WO1999067356A2 (en)	1999-12-29
HUP0102820A2 (hu)	2001-11-28
NL1012434C2 (nl)	2000-01-04
KR20010053141A (ko)	2001-06-25
PL345519A1 (en)	2001-12-17
IS5783A (is)	2000-12-21
ID28046A (id)	2001-05-03
IL140461A0 (en)	2002-02-10
HUP0102820A3 (en)	2003-09-29
ZA200007786B (en)	2001-12-21
BR9911511A (pt)	2001-12-11

Publication	Publication Date	Title
Rajakumar et al.	1997	Use of a novel approach, termed island probing, identifies the Shigella flexneri she pathogenicity island which encodes a homolog of the immunoglobulin A protease-like family of proteins
Leong-Morgenthaler et al.	1991	Lactose metabolism in Lactobacillus bulgaricus: analysis of the primary structure and expression of the genes involved
Lim et al.	1995	Identification of Mycobacterium tuberculosis DNA sequences encoding exported proteins by using phoA gene fusions
Aono et al.	1994	Cloning of organic solvent tolerance gene ostA that determines n-hexane tolerance level in Escherichia coli
KR20130117753A (ko)	2013-10-28	포스포케톨라아제를 포함하는 재조합 숙주 세포
KR20120136349A (ko)	2012-12-18	고가의 화학적 생성물의 미생물 생산, 및 관련 조성물, 방법 및 시스템
KR20140015136A (ko)	2014-02-06	3-히드록시프로피온산 및 다른 생성물의 제조 방법
JP2002511752A (ja)	2002-04-16	生弱毒ワクチン
CN113227364A (zh)	2021-08-06	用于产生熊去氧胆酸及其前体的细胞和方法
Hansen et al.	1992	Use of electroporation to construct isogenic mutants of Haemophilus ducreyi
JP3294288B2 (ja)	2002-06-24	乳酸桿菌由来の新規なプラスミドｐＢＵＬ１及びその誘導体
JP3553065B2 (ja)	2004-08-11	挿入プロモーター含有の組換え乳酸菌とその構築方法
CN114008205A (zh)	2022-02-01	前导序列
US6830901B1 (en)	2004-12-14	Propionibacteruim vector
Dubail et al.	2000	Listeriolysin O as a reporter to identify constitutive and in vivo-inducible promoters in the pathogen Listeria monocytogenes
Mallavia et al.	1991	The genetics of Coxiella burnetii: etiologic agent of Q fever and chronic endocarditis
TWI221854B (en)	2004-10-11	Lac shuttle vectors, kit for expression of a heterologous gene and DNA vaccine carrier containing the same
KR100457879B1 (ko)	2004-11-18	비피도박테리움 유래 신규 플라스미드, 이를 이용한 재조합 발현 벡터 및 형질전환 방법
EP0972835A1 (en)	2000-01-19	Propionibacterium vector
MXPA01000184A (en)	2002-07-25	Propionibacterium vector
Morgan et al.	1997	bctA: a novel pBF4 gene necessary for conjugal transfer in Bacteroides spp.
CN101008014B (zh)	2010-09-08	乳酸乳球菌食品级载体
US20060134135A1 (en)	2006-06-22	Bacterial virulence genes
WO2001077319A2 (en)	2001-10-18	Genetic manipulation of clostridium difficile
KR20040072545A (ko)	2004-08-18	비피도박테리움 롱검 ｍｇ1 유래 신규 플라스미드를함유하는 셔틀벡터