SK65799A3 - Antifreeze polypeptides, isolated nucleic sequence being coding antifreeze polypeptide, method to obtain polypeptides and food product containing them - Google Patents

Antifreeze polypeptides, isolated nucleic sequence being coding antifreeze polypeptide, method to obtain polypeptides and food product containing them Download PDF

Info

Publication number: SK65799A3
Authority: SK; Slovakia
Prior art keywords: leu; polypeptides; antifreeze; polypeptide; ser
Prior art date: 1996-11-19

Application number

SK657-99A

Other languages

English (en)

Slovak (sk)

Inventor

Louise J Byass

Charlotte J Doucet

Richard A Fenn

Andrew J Mcarthur

Christopher M Sidebottom

Margaret F Smallwood

Dawn Warrell

Original Assignee

Unilever Nv

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1996-11-19

Filing date

1997-11-06

Publication date

2000-03-13

1997-11-06 Application filed by Unilever Nv filed Critical Unilever Nv

2000-03-13 Publication of SK65799A3 publication Critical patent/SK65799A3/sk

Links

108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 66
102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 63
229920001184 polypeptide Polymers 0.000 title claims abstract description 60
230000002528 anti-freeze Effects 0.000 title claims abstract description 25
235000013305 food Nutrition 0.000 title claims description 19
238000000034 method Methods 0.000 title claims description 19
244000000626 Daucus carota Species 0.000 claims abstract description 44
235000002767 Daucus carota Nutrition 0.000 claims abstract description 44
235000009508 confectionery Nutrition 0.000 claims abstract description 9
230000000694 effects Effects 0.000 claims description 24
241000196324 Embryophyta Species 0.000 claims description 23
108090000623 proteins and genes Proteins 0.000 claims description 21
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims description 11
239000012634 fragment Substances 0.000 claims description 10
150000001413 amino acids Chemical group 0.000 claims description 9
239000002773 nucleotide Substances 0.000 claims description 9
125000003729 nucleotide group Chemical group 0.000 claims description 9
239000000203 mixture Substances 0.000 claims description 7
235000013311 vegetables Nutrition 0.000 claims description 7
238000004519 manufacturing process Methods 0.000 claims description 6
108010029485 Protein Isoforms Proteins 0.000 claims description 4
102000001708 Protein Isoforms Human genes 0.000 claims description 4
238000004113 cell culture Methods 0.000 claims description 4
238000011065 in-situ storage Methods 0.000 claims description 4
244000005700 microbiome Species 0.000 claims description 4
108700028369 Alleles Proteins 0.000 claims description 3
KFKWRHQBZQICHA-STQMWFEESA-N L-leucyl-L-phenylalanine Natural products CC(C)C[C@H](N)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 KFKWRHQBZQICHA-STQMWFEESA-N 0.000 claims description 2
QYSFWUIXDFJUDW-DCAQKATOSA-N Ser-Leu-Arg Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O QYSFWUIXDFJUDW-DCAQKATOSA-N 0.000 claims description 2
GDUZTEQRAOXYJS-SRVKXCTJSA-N Ser-Phe-Asn Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](CO)N GDUZTEQRAOXYJS-SRVKXCTJSA-N 0.000 claims description 2
108010054813 diprotin B Proteins 0.000 claims description 2
230000009260 cross reactivity Effects 0.000 claims 1
235000003869 genetically modified organism Nutrition 0.000 claims 1
230000002349 favourable effect Effects 0.000 abstract description 2
101710123134 Ice-binding protein Proteins 0.000 description 44
102100034593 Tripartite motif-containing protein 26 Human genes 0.000 description 44
235000015243 ice cream Nutrition 0.000 description 21
239000013078 crystal Substances 0.000 description 18
239000000499 gel Substances 0.000 description 17
102000004169 proteins and genes Human genes 0.000 description 17
239000000523 sample Substances 0.000 description 16
239000007983 Tris buffer Substances 0.000 description 15
239000000047 product Substances 0.000 description 15
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 12
244000061176 Nicotiana tabacum Species 0.000 description 11
235000002637 Nicotiana tabacum Nutrition 0.000 description 11
239000002299 complementary DNA Substances 0.000 description 11
WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 9
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 9
238000001953 recrystallisation Methods 0.000 description 9
229930006000 Sucrose Natural products 0.000 description 8
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 8
238000003556 assay Methods 0.000 description 8
239000005720 sucrose Substances 0.000 description 8
230000009261 transgenic effect Effects 0.000 description 8
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 6
108091026890 Coding region Proteins 0.000 description 6
DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 description 6
210000004027 cell Anatomy 0.000 description 6
239000000284 extract Substances 0.000 description 6
230000002401 inhibitory effect Effects 0.000 description 6
239000002609 medium Substances 0.000 description 6
239000011780 sodium chloride Substances 0.000 description 6
239000006228 supernatant Substances 0.000 description 6
238000012360 testing method Methods 0.000 description 6
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
239000012505 Superdex™ Substances 0.000 description 5
239000004570 mortar (masonry) Substances 0.000 description 5
239000013598 vector Substances 0.000 description 5
108010053481 Antifreeze Proteins Proteins 0.000 description 4
206010020649 Hyperkeratosis Diseases 0.000 description 4
229920002684 Sepharose Polymers 0.000 description 4
238000004458 analytical method Methods 0.000 description 4
239000000872 buffer Substances 0.000 description 4
238000006243 chemical reaction Methods 0.000 description 4
238000010276 construction Methods 0.000 description 4
230000014759 maintenance of location Effects 0.000 description 4
108020004999 messenger RNA Proteins 0.000 description 4
230000036961 partial effect Effects 0.000 description 4
239000013615 primer Substances 0.000 description 4
239000000243 solution Substances 0.000 description 4
108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
240000004808 Saccharomyces cerevisiae Species 0.000 description 3
235000010323 ascorbic acid Nutrition 0.000 description 3
239000011668 ascorbic acid Substances 0.000 description 3
239000012141 concentrate Substances 0.000 description 3
238000000502 dialysis Methods 0.000 description 3
239000013604 expression vector Substances 0.000 description 3
230000008014 freezing Effects 0.000 description 3
238000007710 freezing Methods 0.000 description 3
235000011389 fruit/vegetable juice Nutrition 0.000 description 3
239000005457 ice water Substances 0.000 description 3
239000004615 ingredient Substances 0.000 description 3
229930027917 kanamycin Natural products 0.000 description 3
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 3
229960000318 kanamycin Drugs 0.000 description 3
229930182823 kanamycin A Natural products 0.000 description 3
239000000463 material Substances 0.000 description 3
238000005259 measurement Methods 0.000 description 3
239000008188 pellet Substances 0.000 description 3
230000008569 process Effects 0.000 description 3
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
241000251468 Actinopterygii Species 0.000 description 2
101001050984 Apple stem grooving virus (strain Korea) Putative movement protein Proteins 0.000 description 2
101001050983 Apple stem grooving virus (strain P-209) Probable movement protein Proteins 0.000 description 2
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
240000007124 Brassica oleracea Species 0.000 description 2
235000003899 Brassica oleracea var acephala Nutrition 0.000 description 2
235000011301 Brassica oleracea var capitata Nutrition 0.000 description 2
235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 2
CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
108020004414 DNA Proteins 0.000 description 2
240000009088 Fragaria x ananassa Species 0.000 description 2
108091028043 Nucleic acid sequence Proteins 0.000 description 2
239000002202 Polyethylene glycol Substances 0.000 description 2
108091034057 RNA (poly(A)) Proteins 0.000 description 2
238000012300 Sequence Analysis Methods 0.000 description 2
BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 2
244000269362 Trachymene glaucifolia Species 0.000 description 2
235000016244 Trachymene glaucifolia Nutrition 0.000 description 2
229960005070 ascorbic acid Drugs 0.000 description 2
235000011089 carbon dioxide Nutrition 0.000 description 2
229940008396 carrot extract Drugs 0.000 description 2
238000004587 chromatography analysis Methods 0.000 description 2
NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 2
230000037029 cross reaction Effects 0.000 description 2
235000013399 edible fruits Nutrition 0.000 description 2
238000005227 gel permeation chromatography Methods 0.000 description 2
238000009396 hybridization Methods 0.000 description 2
238000004255 ion exchange chromatography Methods 0.000 description 2
235000013336 milk Nutrition 0.000 description 2
239000008267 milk Substances 0.000 description 2
210000004080 milk Anatomy 0.000 description 2
239000013600 plasmid vector Substances 0.000 description 2
229920002401 polyacrylamide Polymers 0.000 description 2
229920001223 polyethylene glycol Polymers 0.000 description 2
238000000746 purification Methods 0.000 description 2
229910052709 silver Inorganic materials 0.000 description 2
239000004332 silver Substances 0.000 description 2
239000002356 single layer Substances 0.000 description 2
239000007787 solid Substances 0.000 description 2
239000002904 solvent Substances 0.000 description 2
238000003860 storage Methods 0.000 description 2
235000021012 strawberries Nutrition 0.000 description 2
239000000126 substance Substances 0.000 description 2
238000004114 suspension culture Methods 0.000 description 2
230000009466 transformation Effects 0.000 description 2
238000005406 washing Methods 0.000 description 2
239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 1
241000589158 Agrobacterium Species 0.000 description 1
108010039627 Aprotinin Proteins 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
235000016068 Berberis vulgaris Nutrition 0.000 description 1
241000335053 Beta vulgaris Species 0.000 description 1
241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
240000002791 Brassica napus Species 0.000 description 1
235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
244000221633 Brassica rapa subsp chinensis Species 0.000 description 1
235000004977 Brassica sinapistrum Nutrition 0.000 description 1
229920000298 Cellophane Polymers 0.000 description 1
240000008886 Ceratonia siliqua Species 0.000 description 1
235000013912 Ceratonia siliqua Nutrition 0.000 description 1
108020004635 Complementary DNA Proteins 0.000 description 1
102000018832 Cytochromes Human genes 0.000 description 1
108010052832 Cytochromes Proteins 0.000 description 1
108010017826 DNA Polymerase I Proteins 0.000 description 1
102000004594 DNA Polymerase I Human genes 0.000 description 1
239000003155 DNA primer Substances 0.000 description 1
241000208175 Daucus Species 0.000 description 1
229920002307 Dextran Polymers 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
241000238631 Hexapoda Species 0.000 description 1
101001126084 Homo sapiens Piwi-like protein 2 Proteins 0.000 description 1
229930010555 Inosine Natural products 0.000 description 1
UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
102100034343 Integrase Human genes 0.000 description 1
108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
241001424413 Lucia Species 0.000 description 1
235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
239000012901 Milli-Q water Substances 0.000 description 1
108091034117 Oligonucleotide Proteins 0.000 description 1
240000004370 Pastinaca sativa Species 0.000 description 1
235000017769 Pastinaca sativa subsp sativa Nutrition 0.000 description 1
102000035195 Peptidases Human genes 0.000 description 1
108091005804 Peptidases Proteins 0.000 description 1
102100029365 Piwi-like protein 2 Human genes 0.000 description 1
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
108020004511 Recombinant DNA Proteins 0.000 description 1
240000003768 Solanum lycopersicum Species 0.000 description 1
235000021536 Sugar beet Nutrition 0.000 description 1
108010006785 Taq Polymerase Proteins 0.000 description 1
108091036066 Three prime untranslated region Proteins 0.000 description 1
240000008042 Zea mays Species 0.000 description 1
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
235000002017 Zea mays subsp mays Nutrition 0.000 description 1
GYMWQLRSSDFGEQ-ADRAWKNSSA-N [(3e,8r,9s,10r,13s,14s,17r)-13-ethyl-17-ethynyl-3-hydroxyimino-1,2,6,7,8,9,10,11,12,14,15,16-dodecahydrocyclopenta[a]phenanthren-17-yl] acetate;(8r,9s,13s,14s,17r)-17-ethynyl-13-methyl-7,8,9,11,12,14,15,16-octahydro-6h-cyclopenta[a]phenanthrene-3,17-diol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1.O/N=C/1CC[C@@H]2[C@H]3CC[C@](CC)([C@](CC4)(OC(C)=O)C#C)[C@@H]4[C@@H]3CCC2=C\1 GYMWQLRSSDFGEQ-ADRAWKNSSA-N 0.000 description 1
101150055123 afp gene Proteins 0.000 description 1
229960004405 aprotinin Drugs 0.000 description 1
229940072107 ascorbate Drugs 0.000 description 1
230000008901 benefit Effects 0.000 description 1
235000014121 butter Nutrition 0.000 description 1
238000010804 cDNA synthesis Methods 0.000 description 1
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
239000006285 cell suspension Substances 0.000 description 1
230000008859 change Effects 0.000 description 1
230000006835 compression Effects 0.000 description 1
238000007906 compression Methods 0.000 description 1
238000004590 computer program Methods 0.000 description 1
230000001143 conditioned effect Effects 0.000 description 1
235000005822 corn Nutrition 0.000 description 1
239000006059 cover glass Substances 0.000 description 1
235000013365 dairy product Nutrition 0.000 description 1
235000011850 desserts Nutrition 0.000 description 1
230000007613 environmental effect Effects 0.000 description 1
239000006167 equilibration buffer Substances 0.000 description 1
238000002474 experimental method Methods 0.000 description 1
238000000605 extraction Methods 0.000 description 1
235000019197 fats Nutrition 0.000 description 1
239000000706 filtrate Substances 0.000 description 1
239000012467 final product Substances 0.000 description 1
210000003811 finger Anatomy 0.000 description 1
239000000796 flavoring agent Substances 0.000 description 1
235000019634 flavors Nutrition 0.000 description 1
230000007914 freezing tolerance Effects 0.000 description 1
239000012737 fresh medium Substances 0.000 description 1
235000013611 frozen food Nutrition 0.000 description 1
238000001502 gel electrophoresis Methods 0.000 description 1
238000010353 genetic engineering Methods 0.000 description 1
239000011521 glass Substances 0.000 description 1
239000010438 granite Substances 0.000 description 1
239000001963 growth medium Substances 0.000 description 1
238000007542 hardness measurement Methods 0.000 description 1
238000003306 harvesting Methods 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
238000010191 image analysis Methods 0.000 description 1
238000011534 incubation Methods 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
ZPNFWUPYTFPOJU-LPYSRVMUSA-N iniprol Chemical compound C([C@H]1C(=O)NCC(=O)NCC(=O)N[C@H]2CSSC[C@H]3C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC=4C=CC=CC=4)C(=O)N[C@@H](CC=4C=CC(O)=CC=4)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CCCNC(N)=N)NC2=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](N)CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N2[C@@H](CCC2)C(=O)N2[C@@H](CCC2)C(=O)N[C@@H](CC=2C=CC(O)=CC=2)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N2[C@@H](CCC2)C(=O)N3)C(=O)NCC(=O)NCC(=O)N[C@@H](C)C(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N1)C(C)C)[C@@H](C)O)[C@@H](C)CC)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZPNFWUPYTFPOJU-LPYSRVMUSA-N 0.000 description 1
229960003786 inosine Drugs 0.000 description 1
210000003093 intracellular space Anatomy 0.000 description 1
238000005342 ion exchange Methods 0.000 description 1
238000002955 isolation Methods 0.000 description 1
239000010410 layer Substances 0.000 description 1
239000007788 liquid Substances 0.000 description 1
239000007791 liquid phase Substances 0.000 description 1
239000003550 marker Substances 0.000 description 1
230000001404 mediated effect Effects 0.000 description 1
230000003228 microsomal effect Effects 0.000 description 1
239000006870 ms-medium Substances 0.000 description 1
239000005445 natural material Substances 0.000 description 1
229910052757 nitrogen Inorganic materials 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
150000007523 nucleic acids Chemical class 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
239000002245 particle Substances 0.000 description 1
230000037361 pathway Effects 0.000 description 1
230000035699 permeability Effects 0.000 description 1
239000012071 phase Substances 0.000 description 1
125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
230000008488 polyadenylation Effects 0.000 description 1
239000000843 powder Substances 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
235000019833 protease Nutrition 0.000 description 1
238000000734 protein sequencing Methods 0.000 description 1
235000011962 puddings Nutrition 0.000 description 1
230000002829 reductive effect Effects 0.000 description 1
239000011347 resin Substances 0.000 description 1
229920005989 resin Polymers 0.000 description 1
108091008146 restriction endonucleases Proteins 0.000 description 1
238000004007 reversed phase HPLC Methods 0.000 description 1
239000012723 sample buffer Substances 0.000 description 1
235000015067 sauces Nutrition 0.000 description 1
238000012216 screening Methods 0.000 description 1
238000005201 scrubbing Methods 0.000 description 1
238000012163 sequencing technique Methods 0.000 description 1
235000020183 skimmed milk Nutrition 0.000 description 1
235000014347 soups Nutrition 0.000 description 1
230000007480 spreading Effects 0.000 description 1
238000003892 spreading Methods 0.000 description 1
238000010186 staining Methods 0.000 description 1
229910001220 stainless steel Inorganic materials 0.000 description 1
239000010935 stainless steel Substances 0.000 description 1
235000000346 sugar Nutrition 0.000 description 1
239000008399 tap water Substances 0.000 description 1
235000020679 tap water Nutrition 0.000 description 1
239000012085 test solution Substances 0.000 description 1
210000003813 thumb Anatomy 0.000 description 1
238000013518 transcription Methods 0.000 description 1
230000035897 transcription Effects 0.000 description 1
238000012546 transfer Methods 0.000 description 1
238000013519 translation Methods 0.000 description 1
MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 description 1
FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 description 1
235000012141 vanillin Nutrition 0.000 description 1
238000001262 western blot Methods 0.000 description 1
235000013618 yogurt Nutrition 0.000 description 1

Classifications

- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G9/00—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor
- A23G9/32—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds
- A23G9/38—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds containing peptides or proteins
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G9/00—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor
- A23G9/32—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds
- A23G9/42—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds containing plants or parts thereof, e.g. fruits, seeds, extracts
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance

Landscapes

Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Molecular Biology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biochemistry (AREA)
Polymers & Plastics (AREA)
Food Science & Technology (AREA)
Botany (AREA)
General Health & Medical Sciences (AREA)
Biomedical Technology (AREA)
Biophysics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Inorganic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Cell Biology (AREA)
Gastroenterology & Hepatology (AREA)
Medicinal Chemistry (AREA)
Peptides Or Proteins (AREA)
Freezing, Cooling And Drying Of Foods (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Confectionery (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Investigating Or Analysing Biological Materials (AREA)
Physical Water Treatments (AREA)
Hydrogen, Water And Hydrids (AREA)

SK657-99A 1996-11-19 1997-11-06 Antifreeze polypeptides, isolated nucleic sequence being coding antifreeze polypeptide, method to obtain polypeptides and food product containing them SK65799A3 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
EP96308362A EP0843010A1 (de)	1996-11-19	1996-11-19	Frostschutzpolypeptide aus Karotte
PCT/EP1997/006181 WO1998022591A2 (en)	1996-11-19	1997-11-06	Carrot antifreeze polypeptides

Publications (1)

Publication Number	Publication Date
SK65799A3 true SK65799A3 (en)	2000-03-13

Family

ID=8225154

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
SK657-99A SK65799A3 (en)	1996-11-19	1997-11-06	Antifreeze polypeptides, isolated nucleic sequence being coding antifreeze polypeptide, method to obtain polypeptides and food product containing them

Country Status (24)

Country	Link
US (1)	US6797690B1 (de)
EP (2)	EP0843010A1 (de)
JP (1)	JP3474200B2 (de)
CN (1)	CN1191361C (de)
AR (1)	AR013874A1 (de)
AT (1)	ATE319829T1 (de)
AU (1)	AU732169B2 (de)
BR (1)	BR9713984A (de)
CA (1)	CA2272534A1 (de)
CZ (1)	CZ295597B6 (de)
DE (1)	DE69735441T2 (de)
DK (1)	DK0941332T3 (de)
ES (1)	ES2259807T3 (de)
HU (1)	HUP0000258A3 (de)
IL (1)	IL129970A0 (de)
PA (1)	PA8441801A1 (de)
PL (1)	PL333504A1 (de)
PT (1)	PT941332E (de)
SK (1)	SK65799A3 (de)
TR (1)	TR199901695T2 (de)
TW (1)	TW349953B (de)
UY (1)	UY24786A1 (de)
WO (1)	WO1998022591A2 (de)
ZA (1)	ZA9710338B (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB9801410D0 (en)	1998-01-22	1998-03-18	Unilever Plc	Frozen food product
TR200102600T2 (tr)	1999-03-10	2002-03-21	Unilever N.V.	Dondurma.
AU2002352231A1 (en) *	2002-01-04	2003-07-15	Unilever Plc	Antifreeze proteins in vegetables
DE60322106D1 (de) *	2002-12-20	2008-08-21	Unilever Nv	Herstellung von frostschutzprotein
US7615245B2 (en)	2003-04-11	2009-11-10	Cargill, Incorporated	Pellet systems for preparing beverages
AU2006294331A1 (en) *	2005-09-22	2007-03-29	Transfert Plus Societe En Commandite	Plant extract and use thereof as a cryoprotective agent
US20100203215A1 (en) *	2007-06-25	2010-08-12	Nestec S.A.	Producing frozen desserts on the basis of a premixed batch of ingredients
CN101918437B (zh)	2007-11-21	2013-08-14	罗斯基勒大学	具有冰结合活性的多肽
RU2380947C1 (ru) *	2008-12-24	2010-02-10	Государственное образовательное учреждение высшего профессионального образования "Московский государственный университет прикладной биотехнологии" МГУПБ	Способ получения экстракта белка, структурирующего лед из клубней топинамбура
EP2397483A1 (de) *	2009-02-13	2011-12-21	Kaneka Corporation	Pflanzenextrakt mit gefrierschutzstoff und herstellungsverfahren dafür
JP2010285359A (ja) *	2009-06-09	2010-12-24	Kaneka Corp	植物由来氷結晶化阻害剤
WO2013151701A1 (en) *	2012-04-05	2013-10-10	Arborgen Inc.	Improvement of freeze and drought tolerance in plants
CN107163132A (zh) *	2017-06-23	2017-09-15	福州大学	一种基于葡聚糖修饰的抗冻糖肽的制备方法和应用
CN107840874A (zh) *	2017-12-11	2018-03-27	江南大学	一种重组胡萝卜抗冻蛋白基因CaAFP及其应用
CN109295250B (zh) *	2018-10-31	2022-03-22	四川华汉三创生物科技有限公司	一种食源性植物过敏原成分的检测试剂盒和方法
MX2023015332A (es) *	2021-06-15	2024-03-22	Globachem Nv	Agente de proteccion de planta a base de proteinas anticongelantes.
FR3155238A1 (fr)	2023-11-09	2025-05-16	Renault S.A.S	Fluide de refroidissement comprenant au moins une protéine antigel

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU648028B2 (en)	1989-05-10	1994-04-14	Dna Plant Technology Corporation	Antifreeze polypeptides
US5118792A (en)	1989-05-10	1992-06-02	Dna Plant Technology Corporation	Ice crystal growth suppression polypeptides and method of making
US5849537A (en) *	1989-09-19	1998-12-15	Miller Brewing Company	Method of expressing antifreeze proteins in yeast
FI85286C (fi) *	1989-11-23	1992-03-25	Kemira Oy	Koeldbestaendighet foerbaettrande rekombinant- dna-molekyler.
ATE268815T1 (de) *	1991-06-13	2004-06-15	Microstar Biotech Inc	Kältetoleranz bei pflanzen
WO1994003617A1 (en)	1992-07-29	1994-02-17	Unilever N.V.	Process for producing anti-freeze peptides
US5837545A (en) *	1993-01-21	1998-11-17	Research Corporation Technologies, Inc.	Genes, polypeptides, and compositions for cold tolerance in plants
US5676985A (en)	1994-10-12	1997-10-14	Hsc Research And Development Limited Partnership	Antifreeze polypeptide-expressing microorganisms useful in fermentation and freezing of foods
CA2261994C (en) *	1996-07-26	2009-02-03	Unilever Plc	Frozen food product containing heat stable antifreeze protein
EP0959689B1 (de) *	1996-07-26	2007-01-17	Unilever N.V.	Gefrorene süsswaren

1996
- 1996-11-19 EP EP96308362A patent/EP0843010A1/de not_active Withdrawn
1997
- 1997-11-06 AU AU55509/98A patent/AU732169B2/en not_active Ceased
- 1997-11-06 TR TR1999/01695T patent/TR199901695T2/xx unknown
- 1997-11-06 PL PL97333504A patent/PL333504A1/xx unknown
- 1997-11-06 CA CA002272534A patent/CA2272534A1/en not_active Abandoned
- 1997-11-06 DE DE69735441T patent/DE69735441T2/de not_active Expired - Lifetime
- 1997-11-06 HU HU0000258A patent/HUP0000258A3/hu unknown
- 1997-11-06 CN CNB97181371XA patent/CN1191361C/zh not_active Expired - Fee Related
- 1997-11-06 DK DK97951868T patent/DK0941332T3/da active
- 1997-11-06 IL IL12997097A patent/IL129970A0/xx unknown
- 1997-11-06 CZ CZ19991777A patent/CZ295597B6/cs not_active IP Right Cessation
- 1997-11-06 ES ES97951868T patent/ES2259807T3/es not_active Expired - Lifetime
- 1997-11-06 BR BR9713984-0A patent/BR9713984A/pt not_active Application Discontinuation
- 1997-11-06 EP EP97951868A patent/EP0941332B8/de not_active Expired - Lifetime
- 1997-11-06 SK SK657-99A patent/SK65799A3/sk unknown
- 1997-11-06 AT AT97951868T patent/ATE319829T1/de not_active IP Right Cessation
- 1997-11-06 PT PT97951868T patent/PT941332E/pt unknown
- 1997-11-06 JP JP52315098A patent/JP3474200B2/ja not_active Expired - Fee Related
- 1997-11-06 WO PCT/EP1997/006181 patent/WO1998022591A2/en not_active Ceased
- 1997-11-06 US US09/308,140 patent/US6797690B1/en not_active Expired - Fee Related
- 1997-11-17 ZA ZA9710338A patent/ZA9710338B/xx unknown
- 1997-11-18 AR ARP970105377A patent/AR013874A1/es unknown
- 1997-11-19 PA PA19978441801A patent/PA8441801A1/es unknown
- 1997-11-19 UY UY24786A patent/UY24786A1/es unknown
- 1997-12-15 TW TW086118913A patent/TW349953B/zh active

Also Published As

Publication number	Publication date
DE69735441T2 (de)	2006-08-24
CZ295597B6 (cs)	2005-08-17
ZA9710338B (en)	1999-05-17
ES2259807T3 (es)	2006-10-16
JP3474200B2 (ja)	2003-12-08
EP0941332A2 (de)	1999-09-15
PA8441801A1 (es)	2000-05-24
EP0843010A1 (de)	1998-05-20
CN1244895A (zh)	2000-02-16
IL129970A0 (en)	2000-02-29
EP0941332B8 (de)	2006-05-17
AU732169B2 (en)	2001-04-12
EP0941332B1 (de)	2006-03-08
WO1998022591A2 (en)	1998-05-28
US6797690B1 (en)	2004-09-28
TR199901695T2 (xx)	1999-10-21
DE69735441D1 (de)	2006-05-04
BR9713984A (pt)	2000-02-08
JP2000513578A (ja)	2000-10-17
CN1191361C (zh)	2005-03-02
PL333504A1 (en)	1999-12-20
CZ177799A3 (cs)	1999-11-17
HUP0000258A3 (en)	2002-02-28
TW349953B (en)	1999-01-11
AU5550998A (en)	1998-06-10
HUP0000258A2 (en)	2000-07-28
CA2272534A1 (en)	1998-05-28
WO1998022591A3 (en)	1998-07-30
PT941332E (pt)	2006-07-31
ATE319829T1 (de)	2006-03-15
DK0941332T3 (da)	2006-05-15
AR013874A1 (es)	2001-01-31
UY24786A1 (es)	1997-12-16

Publication	Publication Date	Title
JP4175520B2 (ja)	2008-11-05	冷凍菓子製品
US6090917A (en)	2000-07-18	Frozen food product
EP0941332B1 (de)	2006-03-08	Frostschutzpolypeptide aus karotte
CA2257115C (en)	2007-10-09	Spruce budworm antifreeze proteins, genes and methods of using same
US6852841B1 (en)	2005-02-08	Frozen food product
CA2296005C (en)	2011-11-15	Tenebrio antifreeze proteins
CA2261314C (en)	2006-01-03	Frozen food with antifreeze peptides
MXPA99000952A (en)	2000-01-01	Frozen food product containing heat stable antifreeze protein